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11. 发明授权

US6146865A Nucleic acids encoding polypeptides having pyranose oxidase activity 失效
标题翻译：编码具有吡喃糖氧化酶活性的多肽的核酸
公开(公告)号：US6146865A
公开(公告)日：2000-11-14
申请号：US305381
申请日：1999-05-05
申请人： Soren Christensen , Soren Flensted Lassen , Palle Schneider
发明人： Soren Christensen , Soren Flensted Lassen , Palle Schneider
IPC分类号： C12N9/04 , C07H21/04 , C12N1/20 , C12N5/00 , C12N15/00
CPC分类号： C12N9/0006
摘要： The present invention relates to isolated nucleic acid sequences encoding polypeptides having pyranose oxidase activity. The invention also relates to nucleic acid constructs, vectors and host cells containing the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
摘要翻译：本发明涉及编码具有吡喃糖氧化酶活性的多肽的分离的核酸序列。本发明还涉及含有核酸序列的核酸构建体，载体和宿主细胞以及用于产生多肽的重组方法。

12. 发明授权

US08614079B2 Polypeptides having endoglucanase activity and polynucleotides encoding same 有权
公开(公告)号：US08614079B2
公开(公告)日：2013-12-24
申请号：US13597121
申请日：2012-08-28
申请人： Soren Flensted Lassen , Paul Harris , Elena Vlasenko , Kristian Krogh
发明人： Soren Flensted Lassen , Paul Harris , Elena Vlasenko , Kristian Krogh
IPC分类号： C12N15/52 , C12N15/82 , C12N15/00 , C12N9/00
CPC分类号： C12N9/2434 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

13. 发明申请

US20090092707A1 POLYPEPTIDES OF ALICYCLOBACILLUS SP. 失效
标题翻译：藻类多糖
公开(公告)号：US20090092707A1
公开(公告)日：2009-04-09
申请号：US12185413
申请日：2008-08-04
申请人： Reinhard Wilting , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Reinhard Wilting , Soren Flensted Lassen , Peter Rahbek Ostergaard
IPC分类号： A23L1/48 , C12N9/50 , C11D3/386
CPC分类号： C12N9/0004 , C07K14/195 , C07K14/32 , C12N9/18 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/52 , C12N9/86 , C12N9/90 , C12R1/01 , C12Y301/01058 , C12Y302/01096 , C12Y305/02006
摘要： Isolated polypeptides are disclosed selected from the group consisting of: (a) polypeptides comprising an amino acid sequence which has at least 90% identity with a sequence of a mature polypeptide comprised in the group of SEQ ID NO: 26 to SEQ ID NO:50; (b) polypeptides which are encoded a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide. (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide wherein the polypeptides have a function of the corresponding mature polypeptides comprised in SEQ ID NO:26 to SEQ ID NO:50
摘要翻译：公开了分离的多肽，其选自：（a）包含与SEQ ID NO：26至SEQ ID NO：50组中包含的成熟多肽的序列具有至少90％同一性的氨基酸序列的多肽 ; （b）编码在高严格条件下与选自以下的多核苷酸探针杂交的核苷酸序列的多肽：（i）与选自SEQ ID NO：1至SEQ ID NO：1至SEQ ID NO：1的区域的核苷酸序列的互补链编号NO：25编码成熟多肽。（ii）选自编码成熟多肽的SEQ ID NO：1至SEQ ID NO：25的区域的核苷酸序列中包含的cDNA序列的互补链，其中所述多肽具有包含在其中的相应成熟多肽的功能 SEQ ID NO：26至SEQ ID NO：50

14. 发明申请

US20080286415A1 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US20080286415A1
公开(公告)日：2008-11-20
申请号：US11570913
申请日：2005-06-17
申请人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Oestergaard , Morten Fischer
发明人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Oestergaard , Morten Fischer
IPC分类号： A23K1/165 , C12N9/50 , C12N15/11 , C12N15/00 , C12N5/06 , C12N5/04 , A01H5/00 , A01K67/027 , C12P21/04 , C12N1/20
CPC分类号： C12N9/52 , A23J3/346 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C11D3/38609 , C11D3/38618
摘要： Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
摘要翻译：衍生自Nocardiopsis dassonvillei subsp。 dassonvillei DSM 43235，Nocardiopsis prasina DSM 15649，Nocardiopsis prasina（以前的alba）DSM 14010 Nocardiopsis sp。 DSM 16424，Nocardiopsis alkaliphila DSM 44657和Nocardiopsis lucentensis DSM 44048以及同源蛋白酶; 其在各种宿主中的重组生产，包括转基因植物和非人类动物，以及它们在动物饲料和洗涤剂中的应用。蛋白酶是酸稳定的，碱稳定的和/或热稳定的。

15. 发明申请

US20080145912A1 Endoglucanases 审中-公开
公开(公告)号：US20080145912A1
公开(公告)日：2008-06-19
申请号：US11740076
申请日：2007-04-25
申请人： Martin Schulein , Torben Henriksen , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
发明人： Martin Schulein , Torben Henriksen , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号： C12N9/18 , C07H21/04 , C12N15/00 , D06M16/00
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1 2 3 4 5 6 7 8 9 10 11 12 Trp Xaa 13 14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1 2 3 4 5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

16. 发明授权

US09279114B2 Proteases 有权
公开(公告)号：US09279114B2
公开(公告)日：2016-03-08
申请号：US13490559
申请日：2012-06-07
申请人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Øestergaard , Morten Fischer
发明人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Øestergaard , Morten Fischer
IPC分类号： C12N9/52 , A23J3/34 , A23K1/165 , A23K1/18 , C11D3/386
CPC分类号： C12N9/52 , A23J3/346 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C11D3/38609 , C11D3/38618
摘要： Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.

17. 发明授权

US08465959B2 Proteases and methods for producing them 失效
标题翻译：蛋白酶及其制备方法
公开(公告)号：US08465959B2
公开(公告)日：2013-06-18
申请号：US12147683
申请日：2008-06-27
申请人： Soren Flensted Lassen
发明人： Soren Flensted Lassen
IPC分类号： C12N9/12
CPC分类号： C11D3/38618 , A23K20/174 , A23K20/189 , A23K20/30 , C11D3/38609 , C12N9/58
摘要： A secreted mature polypeptide derived from an S2A or S1E protease is described which after maturation has protease activity, which polypeptide when expressed and before maturation comprises a heterologous pro-region. Further, a method for producing polypeptide is described including the step of cultivating a recombinant host cell including a polynucleotide encoding a secreted mature polypeptide which after maturation has protease activity, which polypeptide when expressed and before maturation includes a heterologous pro-region derived from an S2A or S1E protease to produce a supermatant including the polypeptide. The polypeptide may also be recovered.
摘要翻译：描述了衍生自S2A或S1E蛋白酶的分泌的成熟多肽，其在成熟后具有蛋白酶活性，当所述多肽在成熟时表达包含异源原区。此外，描述了生产多肽的方法，其包括培养重组宿主细胞的步骤，所述重组宿主细胞包含编码分泌的成熟多肽的多核苷酸，其在成熟后具有蛋白酶活性，所述多肽在成熟时包括来源于S2A的异源原区或S1E蛋白酶以产生包括该多肽的超表观物质。也可以回收多肽。

18. 发明授权

US08377677B2 Protease variants 有权
公开(公告)号：US08377677B2
公开(公告)日：2013-02-19
申请号：US12984826
申请日：2011-01-05
申请人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hylling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
IPC分类号： C12N9/52 , C11D3/386 , C12N15/57 , C12N15/73
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.

19. 发明申请

US20120321746A1 Proteases 审中-公开
标题翻译：蛋白酶
公开(公告)号：US20120321746A1
公开(公告)日：2012-12-20
申请号：US13490559
申请日：2012-06-07
申请人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Ostergaard , Morten Fischer
发明人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Ostergaard , Morten Fischer
IPC分类号： C12N9/52 , C12N15/57 , C12N15/63 , A23K1/165 , C12N5/10 , C12N1/15 , C12N1/19 , C12S3/14 , C12N1/21
CPC分类号： C12N9/52 , A23J3/346 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C11D3/38609 , C11D3/38618
摘要： Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
摘要翻译：衍生自Nocardiopsis dassonvillei subsp。 dassonvillei DSM 43235，Nocardiopsis prasina DSM 15649，Nocardiopsis prasina（以前的alba）DSM 14010 Nocardiopsis sp。 DSM 16424，Nocardiopsis alkaliphila DSM 44657和Nocardiopsis lucentensis DSM 44048以及同源蛋白酶; 其在各种宿主中的重组生产，包括转基因植物和非人类动物，以及它们在动物饲料和洗涤剂中的应用。蛋白酶是酸稳定的，碱稳定的和/或热稳定的。

20. 发明授权

US07901918B2 Proteases and variants thereof 有权
标题翻译：蛋白酶及其变体
公开(公告)号：US07901918B2
公开(公告)日：2011-03-08
申请号：US12351454
申请日：2009-01-09
申请人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
IPC分类号： C12N9/54 , C11D3/386 , C12N15/57 , C12N15/74 , C12N15/75
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译：本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

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