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11. 发明授权

US08541209B2 L-proline cis-4-hydroxylase and use thereof to produce cis-4-hydroxy-L-proline 有权
标题翻译： L-脯氨酸顺式-4-羟化酶及其用于产生顺式-4-羟基-L-脯氨酸的用途
公开(公告)号：US08541209B2
公开(公告)日：2013-09-24
申请号：US12992417
申请日：2009-05-12
申请人： Kuniki Kino , Ryotaro Hara
发明人： Kuniki Kino , Ryotaro Hara
IPC分类号： C12P13/24
CPC分类号： C12P13/24 , C12N9/0071 , C12Y114/11002
摘要： Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.
摘要翻译：开发经济有效地生产顺式-4-羟基-L-脯氨酸的方法。本发明提供L-脯氨酸顺式-4-羟化酶。这种酶可以衍生自莲花根瘤菌，中华根瘤菌或苜蓿根瘤菌，中华根瘤菌（Sinorhizobium meliloti）。本发明提供了使用该酶从L-脯氨酸生产顺式-4-羟基-L-脯氨酸的方法。本发明提供了含有编码该酶的多核苷酸和含有载体的转化体的重组载体。

12. 发明申请

US20110091942A1 PROCESS FOR PRODUCTION OF CIS-4-HYDROXY-L-PROLINE 有权
标题翻译：生产CIS-4-羟基-L-丙氨酸的方法
公开(公告)号：US20110091942A1
公开(公告)日：2011-04-21
申请号：US12992417
申请日：2009-05-12
申请人： Kuniki Kino , Ryotaro Hara
发明人： Kuniki Kino , Ryotaro Hara
IPC分类号： C12P13/24 , C12N9/02
CPC分类号： C12P13/24 , C12N9/0071 , C12Y114/11002
摘要： Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.
摘要翻译：开发经济有效地生产顺式-4-羟基-L-脯氨酸的方法。本发明提供L-脯氨酸顺式-4-羟化酶。这种酶可以衍生自莲花根瘤菌，中华根瘤菌或苜蓿根瘤菌，中华根瘤菌（Sinorhizobium meliloti）。本发明提供了使用该酶从L-脯氨酸生产顺式-4-羟基-L-脯氨酸的方法。本发明提供了含有编码该酶的多核苷酸和含有载体的转化体的重组载体。

13. 发明授权

US07223559B2 Plasmids for chromosomal recombination of Escherichia coli 失效
标题翻译：用于大肠杆菌染色体重组的质粒
公开(公告)号：US07223559B2
公开(公告)日：2007-05-29
申请号：US10240712
申请日：2001-04-06
申请人： Junicho Takano , Kuniki Kino , Satoru Furukawa
发明人： Junicho Takano , Kuniki Kino , Satoru Furukawa
IPC分类号： C12P21/02
CPC分类号： C12N15/70
摘要： A temperature-sensitive plasmid which is capable of autonomous replication in Escherichia coli K-12 at 10–30° C., but, at a temperature of 33° C. or more, is incapable of autonomous replication in Escherichia coli K-12 or is distributed unhomogeneously upon the cell division of Escherichia coli K-12, thereby not to be stably carried within cells of Escherichia coli K-12 under said temperature, and which is incapable of autonomous replication in a microorganism belonging to the genus Escherichia other than Escherichia coli K-12 or is distributed unhomogeneously upon cell division of said microorganism at any temperature, thereby not to be stably carried within cells of said microorganism.
摘要翻译：能够在10-30℃，但在33℃或更高温度下能够在大肠杆菌K-12中自主复制的温度敏感性质粒不能在大肠杆菌K-12或在大肠杆菌K-12的细胞分裂时不均匀地分布，因此在所述温度下不能稳定地携带在大肠杆菌K-12的细胞内，并且不能在属于埃希氏杆菌属的大肠杆菌属的微生物中自主复制大肠杆菌K-12或在任何温度下细胞分裂细胞时分布不均匀，从而不能在所述微生物的细胞内稳定携带。

14. 发明申请

US20060199249A1 Method for protein production 失效
标题翻译：蛋白质生产方法
公开(公告)号：US20060199249A1
公开(公告)日：2006-09-07
申请号：US11366285
申请日：2006-03-02
申请人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
发明人： Daisuke Koma , Kuniki Kino , Toshiya Sawai
IPC分类号： C12P21/06 , C12N15/74 , C12N1/21 , C07K14/185
CPC分类号： C12P21/02 , C12N9/1096
摘要： To provide a novel method for obtaining a protein of interest efficiently in a soluble form when the protein is produced by expressing a foreign gene in host cells such as microorganisms etc. In a method for, by culturing host cells into which a foreign gene has been introduced, producing a protein encoded by the foreign gene, after induction of expression, recombinant cells are cultured in the temperature range lower than or equal to the upper temperature limit for growth of the host cells and higher than 5 degrees below the upper temperature limit for growth of the host cells.
摘要翻译：提供了通过在诸如微生物等宿主细胞中表达外源基因而产生蛋白质时，以可溶形式有效获得感兴趣的蛋白质的新方法。在通过培养外源基因已经被培养的宿主细胞的方法中引入，产生由外源基因编码的蛋白质，在诱导表达后，重组细胞在低于或等于宿主细胞生长的上限温度的温度范围内培养，高于上限温度以上5度以上宿主细胞的生长。

15. 发明申请

US20050037199A1 Powdery rubber having unevened surface and rubber compositions and tires using the same 失效
标题翻译：具有不均匀表面和橡胶组合物的粉状橡胶和使用其的轮胎
公开(公告)号：US20050037199A1
公开(公告)日：2005-02-17
申请号：US10497476
申请日：2002-11-28
申请人： Kuniki Kino , Kohtaro Kirimura , Masahiro Hojo , Yuichi Ishino
发明人： Kuniki Kino , Kohtaro Kirimura , Masahiro Hojo , Yuichi Ishino
IPC分类号： B29B17/02 , B29B17/04 , B60C1/00 , C08J11/10 , C08J11/18 , B32B5/16
CPC分类号： B29B17/04 , B29B17/02 , B29B17/0404 , B29K2021/00 , B29L2030/00 , B60C1/00 , B60C1/0025 , C08J11/10 , C08J11/105 , C08J2321/00 , Y02W30/622 , Y02W30/625 , Y02W30/68 , Y02W30/702 , Y10T428/2982 , Y10T428/2991
摘要： A powder rubber obtained by crushing a waste rubber comprising various kinds of rubbers is subjected to treatment of making unevenness on a surface thereof. A method in which a microorganism growing with a rubber being an only carbon source and powder rubber having an average particle diameter of 1 mm or less are added to a culture medium to cultivate the microorganism can be applied as treatment of making the unevenness. The powder rubber is decomposed by this method, and the surface area is increased, whereby a rubber composition which is excellent in abrasion resistance, fracture characteristics and crack growth resistance can be obtained. A WU-YS05 strain (Patent Microorganism Depository Center: Accession number: FERM P-18660) belonging to Pseudonocardiaceae is effective as the microorganism.
摘要翻译：通过对包含各种橡胶的废橡胶进行粉碎而得到的粉末橡胶在其表面上进行不均匀处理。可以将作为唯一碳源的橡胶生长的微生物和平均粒径为1mm以下的粉末橡胶添加到培养基中以培养微生物的方法，作为使不均匀性的处理。通过该方法分解粉末状橡胶，表面积增加，得到耐磨性，断裂特性，耐裂纹扩展性优异的橡胶组合物。作为微生物，属于假心内科的WU-YS05菌株（专利微生物保藏中心：登录号：FERM P-18660）是有效的。

16. 发明授权

US06344347B1 Method for producing L-amino acids by fermentation 有权
标题翻译：通过发酵生产L-氨基酸的方法
公开(公告)号：US06344347B1
公开(公告)日：2002-02-05
申请号：US09663795
申请日：2000-09-18
申请人： Kuniki Kino , Tetsuya Abe
发明人： Kuniki Kino , Tetsuya Abe
IPC分类号： C12P1324
CPC分类号： C12P13/24 , C12P13/04 , C12R1/19 , Y10S435/83 , Y10S435/832 , Y10S435/839 , Y10S435/843 , Y10S435/849 , Y10S435/88 , Y10S435/881
摘要： The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising culturing in a medium a microorganism having an ability to produce the L-amino acid and having resistance to a DNA gyrase inhibitor or a microorganism having an ability to produce the L-amino acid and having both resistance to a DNA gyrase inhibitor and resistance to an aminoquinoline derivative, producing and accumulating the L-amino acid therein and recovering the L-amino acid therefrom.
摘要翻译：本发明提供了用于制备可用作药物，化学试剂，食品材料和饲料添加剂的L-氨基酸的工业上有效的方法，所述方法包括在培养基中培养具有产生L-氨基酸的能力的微生物，并具有对DNA促旋酶抑制剂或具有产生L-氨基酸的能力并对DNA促旋酶抑制剂具有抗性和对氨基喹啉衍生物具有抗性的微生物的抗性，其中产生和积累L-氨基酸并回收L-氨基酸酸。

17. 发明授权

US5376538A Process for producing L-threonine with strains of E coli resistant to phenylalanine and leucine 失效
标题翻译：用于生产具有对苯丙氨酸和亮氨酸耐药的大肠杆菌菌株的L-苏氨酸的方法
公开(公告)号：US5376538A
公开(公告)日：1994-12-27
申请号：US160668
申请日：1993-12-02
申请人： Kuniki Kino , Junichi Takano , Kazuyuki Okamoto , Yoshiyuki Kuratsu
发明人： Kuniki Kino , Junichi Takano , Kazuyuki Okamoto , Yoshiyuki Kuratsu
IPC分类号： C12P13/08 , C12R1/19
CPC分类号： C12P13/08
摘要： Disclosed is a process for producing L-threonine, which comprises culturing an L-threonine-producing microorganism belonging to the genus Escherichia and having a resistance to at least one of L-phenylalanine and L-leucine in a medium until L-threonine is produced and accumulated in the culture, and recovering L-threonine therefrom.
摘要翻译：公开了一种L-苏氨酸的制造方法，其包括在培养基中培养属于埃希氏杆菌属的L-苏氨酸生产微生物，并且在培养基中具有对L-苯丙氨酸和L-亮氨酸中的至少一种的抗性，直至产生L-苏氨酸并在培养物中积累，从其中回收L-苏氨酸。

18. 发明授权

US07344777B2 Powder rubber subjected to treatment of making unevenness on surface thereof and rubber composition using the same and tire 失效
标题翻译：在其表面上进行不均匀处理的粉末橡胶和使用其的轮胎的橡胶组合物
公开(公告)号：US07344777B2
公开(公告)日：2008-03-18
申请号：US10497476
申请日：2002-11-28
申请人： Kuniki Kino , Kohtaro Kirimura , Masahiro Hojo , Yuichi Ishino
发明人： Kuniki Kino , Kohtaro Kirimura , Masahiro Hojo , Yuichi Ishino
IPC分类号： B32B5/66
CPC分类号： B29B17/04 , B29B17/02 , B29B17/0404 , B29K2021/00 , B29L2030/00 , B60C1/00 , B60C1/0025 , C08J11/10 , C08J11/105 , C08J2321/00 , Y02W30/622 , Y02W30/625 , Y02W30/68 , Y02W30/702 , Y10T428/2982 , Y10T428/2991
摘要： A powder rubber obtained by crushing a waste rubber comprising various kinds of rubbers is subjected to treatment of making unevenness on a surface thereof. A method in which a microorganism growing with a rubber being an only carbon source and powder rubber having an average particle diameter of 1 mm or less are added to a culture medium to cultivate the microorganism can be applied as treatment of making the unevenness. The powder rubber is decomposed by this method, and the surface area is increased, whereby a rubber composition which is excellent in abrasion resistance, fracture characteristics and crack growth resistance can be obtained. A WU-YS05 strain (Patent Microorganism Depository Center: Accession number: FERM P-18660) belonging to Pseudonocardiaceae is effective as the microorganism.
摘要翻译：通过对包含各种橡胶的废橡胶进行粉碎而得到的粉末橡胶在其表面上进行不均匀处理。可以将作为唯一碳源的橡胶生长的微生物和平均粒径为1mm以下的粉末橡胶添加到培养基中以培养微生物的方法，作为使不均匀性的处理。通过该方法分解粉末状橡胶，表面积增加，得到耐磨性，断裂特性，耐裂纹扩展性优异的橡胶组合物。作为微生物，属于假心内科的WU-YS05菌株（专利微生物保藏中心：登录号：FERM P-18660）是有效的。

19. 发明申请

US20060194302A1 Method for producing amino acids by fermentation 有权
标题翻译：通过发酵生产氨基酸的方法
公开(公告)号：US20060194302A1
公开(公告)日：2006-08-31
申请号：US11414175
申请日：2006-05-01
申请人： Kuniki Kino , Tetsuya Abe
发明人： Kuniki Kino , Tetsuya Abe
IPC分类号： C12N1/20 , C12N1/12
CPC分类号： C12P13/04 , C12P13/24 , C12R1/19
摘要： The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.
摘要翻译：本发明提供了选自L-丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-甲硫氨酸，L-苯丙氨酸，L-脯氨酸，甘氨酸，L-丝氨酸的氨基酸的制备方法，L-苏氨酸，L-半胱氨酸，L-酪氨酸，L-天冬酰胺，L-谷氨酰胺，L-赖氨酸，L-组氨酸，L-精氨酸，L-天冬氨酸和L-谷氨酸，可用作药物，食品原料和饲料添加剂，该方法包括在培养基中培养具有产生氨基酸并具有氨基喹啉衍生物的能力的微生物，在培养物中产生和积累本发明中的氨基酸，以及从培养物中回收氨基酸。

20. 发明授权

US06706517B2 Method for producing L-amino acids by fermentation 有权
标题翻译：通过发酵生产L-氨基酸的方法
公开(公告)号：US06706517B2
公开(公告)日：2004-03-16
申请号：US10024190
申请日：2001-12-21
申请人： Kuniki Kino , Tetsuya Abe
发明人： Kuniki Kino , Tetsuya Abe
IPC分类号： C12N120
CPC分类号： C12P13/24 , C12P13/04 , C12R1/19 , Y10S435/83 , Y10S435/832 , Y10S435/839 , Y10S435/843 , Y10S435/849 , Y10S435/88 , Y10S435/881
摘要： The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising culturing in a medium a microorganism having an ability to produce the L-amino acid and having resistance to a DNA gyrase inhibitor or a microorganism having an ability to produce the L-amino acid and having both resistance to a DNA gyrase inhibitor and resistance to an aminoquinoline derivative, producing and accumulating the L-amino acid therein and recovering the L-amino acid therefrom.
摘要翻译：本发明提供了用于制备可用作药物，化学试剂，食品材料和饲料添加剂的L-氨基酸的工业上有效的方法，所述方法包括在培养基中培养具有产生L-氨基酸的能力的微生物，并具有对DNA促旋酶抑制剂或具有产生L-氨基酸的能力并对DNA促旋酶抑制剂具有抗性和对氨基喹啉衍生物具有抗性的微生物的抗性，其中产生和积累L-氨基酸并回收L-氨基酸酸。

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