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    • 1. 发明授权
    • Plasmids for chromosomal recombination of Escherichia coli
    • 用于大肠杆菌染色体重组的质粒
    • US07223559B2
    • 2007-05-29
    • US10240712
    • 2001-04-06
    • Junicho TakanoKuniki KinoSatoru Furukawa
    • Junicho TakanoKuniki KinoSatoru Furukawa
    • C12P21/02
    • C12N15/70
    • A temperature-sensitive plasmid which is capable of autonomous replication in Escherichia coli K-12 at 10–30° C., but, at a temperature of 33° C. or more, is incapable of autonomous replication in Escherichia coli K-12 or is distributed unhomogeneously upon the cell division of Escherichia coli K-12, thereby not to be stably carried within cells of Escherichia coli K-12 under said temperature, and which is incapable of autonomous replication in a microorganism belonging to the genus Escherichia other than Escherichia coli K-12 or is distributed unhomogeneously upon cell division of said microorganism at any temperature, thereby not to be stably carried within cells of said microorganism.
    • 能够在10-30℃,但在33℃或更高温度下能够在大肠杆菌K-12中自主复制的温度敏感性质粒不能在大肠杆菌K-12或 在大肠杆菌K-12的细胞分裂时不均匀地分布,因此在所述温度下不能稳定地携带在大肠杆菌K-12的细胞内,并且不能在属于埃希氏杆菌属的大肠杆菌属的微生物中自主复制 大肠杆菌K-12或在任何温度下细胞分裂细胞时分布不均匀,从而不能在所述微生物的细胞内稳定携带。