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91. 发明公开

US20230218690A1 ONCOLYTIC ADENOVIRUS WITH REPLICATION SELECTIVITY BASED ON STATUS OF P53 TRANSCRIPTIONAL ACTIVITY 审中-公开
公开(公告)号：US20230218690A1
公开(公告)日：2023-07-13
申请号：US18151081
申请日：2023-01-06
申请人： Salk Institute for Biological Studies
发明人： William Partlo , Clodagh O'Shea
IPC分类号： A61K35/761 , C12N7/00
CPC分类号： A61K35/761 , C12N7/00 , C12N2710/10321 , C12N2710/10332 , C12N2830/005 , C12N2830/008 , C12N2830/15
摘要： Oncolytic adenoviruses capable of selectively replicating in tumor cells deficient in p53 transcriptional activity are described. Recombinant adenovirus genomes that encode the p53-selective adenoviruses are also described. The recombinant adenoviruses and adenovirus genomes, or compositions thereof, can be used, for example, to reduce or inhibit tumor progression, or reduce tumor volume in a subject with a tumor having dysregulated p53 activity.

92. 发明申请

US20180066284A1 HIGHLY INDUCIBLE DUAL-PROMOTER LENTIVIRAL TET-ON SYSTEM 审中-公开
公开(公告)号：US20180066284A1
公开(公告)日：2018-03-08
申请号：US15785388
申请日：2017-10-16
申请人： FUNDACIÓN PÚBLICA ANDALUZA PROGRESO Y SALUD , INSTITUTO DE SALUD CARLOS III
发明人： Francisco Martin Molina , Karim Benabdellah El Khlanji , Marien Cobo Pulido , Miguel Garcia Toscano , Pilar Munoz Fernandez
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , A01K2267/03 , A61K48/00 , C12N2740/15043 , C12N2830/005
摘要： The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

93. 发明申请

US20170298387A1 VIRUS-BASED EXPRESSION VECTORS AND USES THEREOF 审中-公开
公开(公告)号：US20170298387A1
公开(公告)日：2017-10-19
申请号：US15514119
申请日：2015-09-25
申请人： The USA, as represented by the Secretary, Department of Health and Human Services
发明人： Bernard MOSS , Linda S. WYATT
IPC分类号： C12N15/86 , A61K35/76 , C12N9/12 , A61K39/145 , A61K48/00 , A61K39/00
CPC分类号： C12N15/86 , A61K35/76 , A61K39/145 , A61K48/0066 , A61K2039/5256 , C12N9/1247 , C12N2710/24122 , C12N2710/24143 , C12N2710/24151 , C12N2710/24171 , C12N2760/16134 , C12N2760/16171 , C12N2799/023 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/005 , C12N2830/55 , C12N2830/60 , C12Y207/07006
摘要： Expression vectors ideal for use in vaccinating individuals against disease based on vaccinia virus and other chordopoxviruses having high expression of recombinant genes and low expression of vector genes in target animals, and low expression of recombinant genes and high expression of vector genes in cells used for propagation.

94. 发明申请

US20170044570A1 Engineered Cellular Pathways for Programmed Autoregulation of Differentiation 审中-公开
标题翻译：用于区分程序自动调节的工程细胞途径
公开(公告)号：US20170044570A1
公开(公告)日：2017-02-16
申请号：US15251894
申请日：2016-08-30
申请人： The Trustees of Princeton University
发明人： Ron Weiss , Ihor Lemischka , Priscilla E. M. Purnick , Christoph Schaniel , Miles Miller , Patrick Guye
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , C12N2800/40 , C12N2830/002 , C12N2830/003 , C12N2830/005 , C12N2830/006 , C12N2830/008 , C12N2830/15 , C12N2830/55 , C12N2830/85
摘要： The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.
摘要翻译：本发明提供用于编程哺乳动物细胞以实现所需功能的组合物和方法。特别地，本发明提供了用于编程干细胞以分化成所需细胞类型的组合物和方法。调节细胞命运调节因子表达的群体感应系统被引入哺乳动物宿主细胞，例如干细胞。群体感测系统通常包括表达细菌群体感知途径的组分的载体，包括催化自身诱导剂的合成的蛋白质和编码自身诱导剂的调节配偶体的基因以及编码细胞命运调节物的基因可操作地连接的载体由自动诱导剂/调节配体复合物诱导的启动子。该系统还可以包含载体，其中编码另外的细胞命运调节子的基因可操作地连接到由响应于第一分化阶段合成的因子诱导的启动子，从而触发第二阶段的分化。

95. 发明申请

US20160326546A1 GENERATION OF LAYERED TRANSCRIPTIONAL CIRCUITRY USING CRISPR SYSTEMS 审中-公开
标题翻译：使用CRISPR系统生成层状转录电路
公开(公告)号：US20160326546A1
公开(公告)日：2016-11-10
申请号：US15146653
申请日：2016-05-04
申请人： Massachusetts Institute of Technology , Raytheon BBN Technologies, Corp.
发明人： Samira Kiani , Ron Weiss , Jacob S. Beal , Mohammad Reza Ebrahimkhani , Zhen Xie , Yinqing Li
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , C12N15/63 , C12N2830/002 , C12N2830/005
摘要： Provided herein, in some embodiments, are modular transcriptional architectures and methods for regulated expression of guide RNAs in cells, such as human cells, which are based on Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems.
摘要翻译：在本文中，在一些实施方案中，是基于聚簇定期间隔回归重复（CRISPR）系统的细胞（例如人细胞）中调控表达指导RNA的模块转录结构和方法。

96. 发明授权

US09458471B2 Engineered cellular pathways for programmed autoregulation of differentiation 有权
标题翻译：用于程序自动调节分化的工程化细胞途径
公开(公告)号：US09458471B2
公开(公告)日：2016-10-04
申请号：US14174475
申请日：2014-02-06
申请人： The Trustees of Princeton University
发明人： Ron Weiss , Ihor Lemischka , Priscilla Purnick , Christoph Schaniel , Miles Miller , Patrick Guye
IPC分类号： C12N15/00 , C12N15/11 , C12N15/09 , C12N15/85
CPC分类号： C12N15/85 , C12N2800/40 , C12N2830/002 , C12N2830/003 , C12N2830/005 , C12N2830/006 , C12N2830/008 , C12N2830/15 , C12N2830/55 , C12N2830/85
摘要： The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.
摘要翻译：本发明提供用于编程哺乳动物细胞以实现所需功能的组合物和方法。特别地，本发明提供了用于编程干细胞以分化成所需细胞类型的组合物和方法。调节细胞命运调节因子表达的群体感应系统被引入哺乳动物宿主细胞，例如干细胞。群体感测系统通常包括表达细菌群体感知途径的组分的载体，包括催化自身诱导剂的合成的蛋白质和编码自身诱导剂的调节配偶体的基因以及编码细胞命运调节物的基因可操作地连接的载体由自动诱导剂/调节配体复合物诱导的启动子。该系统还可以包含载体，其中编码另外的细胞命运调节子的基因可操作地连接到由响应于第一分化阶段合成的因子诱导的启动子，从而触发第二阶段的分化。

97. 发明申请

US20120301922A1 NOVEL P. PASTORIS PASTORIS PROMOTERS, AND THE USE THEREOF TO DIRECT EXPRESSION OF PROTEINS IN YEAST, PREFERABLY USING A HAPLOID MATING STRATEGY 有权
标题翻译：新颖的PASTORIS PASTORIS促销者，以及它们用来直接表达蛋白质在YEAST中，优先使用HAPLOID MATING策略
公开(公告)号：US20120301922A1
公开(公告)日：2012-11-29
申请号：US13542851
申请日：2012-07-06
申请人： James M. Cregg , Ilya I. Tolstorukov
发明人： James M. Cregg , Ilya I. Tolstorukov
IPC分类号： C12N15/113 , C12P21/00 , C12N1/19 , C12N15/12 , C12N15/81
CPC分类号： C12N9/88 , C07K14/39 , C07K16/2809 , C12N9/0006 , C12N9/1205 , C12N15/81 , C12N15/815 , C12N2830/005 , C12N2830/702 , C12P21/02 , C12Y402/01011 , Y02P20/52
摘要： Novel promoters which are derived from P. pastoris pastoris which are inducible or repressible under specific growth conditions are provided. These promoters are useful for regulating the expression of a desired structural gene, e.g., a mammalian polypeptide. Particularly preferred is the use of these novel promoters to regulate gene expression in polyploidal yeast such as diploidal P. pastoris produced by mating or spheroplast fusion.
摘要翻译：提供了在特定生长条件下可诱导或阻抑的来自巴斯德一种巴斯德毕赤酵母的新型启动子。这些启动子可用于调节所需结构基因例如哺乳动物多肽的表达。特别优选使用这些新型启动子来调节多倍体酵母中的基因表达，例如通过交配或原生质球融合产生的二倍体巴斯德毕赤酵母。

98. 发明申请

US20120294838A1 Regulation of endogenous gene expression in cells using zinc finger proteins 审中-公开
标题翻译：使用锌指蛋白调节细胞内源性基因表达
公开(公告)号：US20120294838A1
公开(公告)日：2012-11-22
申请号：US13068878
申请日：2011-05-23
申请人： George Norbert Cox III , Casey Christopher Case , Stephen P. Eisenberg , Eric Edward Jarvis , Sharon Kaye Spratt
发明人： George Norbert Cox III , Casey Christopher Case , Stephen P. Eisenberg , Eric Edward Jarvis , Sharon Kaye Spratt
IPC分类号： C12N15/86 , C12N15/867 , C12N15/861 , C12N15/864 , C12N15/869 , A61K35/12 , A61P35/00 , A61P31/10 , A61P33/02 , A61P31/04 , A61P31/12 , A61P9/10 , A61P9/00 , A61P19/02 , A61P37/00 , A61K48/00 , C12N5/071
CPC分类号： A01K67/0275 , A01K2217/05 , A61K48/00 , A61K48/005 , C07K14/47 , C07K14/52 , C07K2319/00 , C07K2319/71 , C07K2319/81 , C12N15/63 , C12N15/67 , C12N15/8216 , C12N15/85 , C12N2830/002 , C12N2830/005 , C12N2830/008 , C12N2830/85 , C12Q1/66 , C12Q1/6897 , G01N33/5091
摘要： The present invention provides methods for modulating expression of endogenous cellular genes using recombinant zinc finger proteins.
摘要翻译：本发明提供了使用重组锌指蛋白调节内源细胞基因表达的方法。

99. 发明授权

US08129598B2 DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system 有权
标题翻译：通过噬菌体PHIC31重组系统在真核细胞中进行DNA重组
公开(公告)号：US08129598B2
公开(公告)日：2012-03-06
申请号：US10721980
申请日：2003-11-24
申请人： David W. Ow , Richard Calendar , Lynn Thomason
发明人： David W. Ow , Richard Calendar , Lynn Thomason
IPC分类号： C12N5/04
CPC分类号： C12N15/8201 , C12N15/81 , C12N15/85 , C12N15/90 , C12N2800/30 , C12N2830/00 , C12N2830/005 , C12N2830/55 , C12N2830/706 , C12N2840/20
摘要： This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use prokaryotic recombinase polypeptides, such as the ΦC31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombination. Thus, the recombination is irreversible in the absence of additional factors. Eukaryotic cells that contain the recombinase polypeptides, or genes that encode the recombinases, are also provided.
摘要翻译：本发明提供了获得核酸到真核细胞的特异性和稳定整合的方法。本发明利用使用原核重组酶多肽（例如ΦC31整合酶）的位点特异性重组系统，其可以介导重组位点之间的重组，而不是在重组后形成的杂合重组位点之间。因此，在没有其他因素的情况下，重组是不可逆的。还提供了含有重组酶多肽的真核细胞或编码重组酶的基因。

100. 发明申请

US20120029074A1 CONTROLLING TRANSGENE EXPRESSION ACROSS THE SKIN 有权
标题翻译：控制皮肤的转运表达
公开(公告)号：US20120029074A1
公开(公告)日：2012-02-02
申请号：US13263149
申请日：2010-04-01
申请人： Martin Fussenegger , Marc Gitzinger
发明人： Martin Fussenegger , Marc Gitzinger
IPC分类号： A61K31/235 , A61P43/00 , C12N5/10 , A61K31/12 , C12N15/63
CPC分类号： C12N15/8509 , A61K9/0014 , A61K47/10 , C12N2820/55 , C12N2830/002 , C12N2830/005
摘要： The invention relates to the use of a skin permeating compound such as phloretin for controlling transgene expression under control of the Pseudomonas putida DOT-T1E-derived bacterial repressor TtgR, to a vector comprising the genetic code for the repressor TtgR fused to a transactivation or a transrepressor domain, to a vector comprising a TtgR-specific operator sequence (OTtgR), a promoter and a polynucleotide coding for an endogenous or exogenous protein, and to a mammalian cell transiently or constitutively transfected with the mentioned vectors, and to mammals comprising such cells in nano- or microcontainers.
摘要翻译：本发明涉及使用皮肤渗透化合物如根皮素用于控制恶臭假单胞菌DOT-T1E衍生的细菌阻遏物TtgR的控制下的转基因表达，所述载体包含与转录激活融合的阻遏物TtgR的遗传密码包含TtgR特异性操纵子序列（OTtgR），启动子和编码内源或外源蛋白的多核苷酸的载体，以及用所述载体瞬时或组成型转染的哺乳动物细胞，以及包含这些细胞的哺乳动物在纳米或微型容器中。

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