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1. 发明申请

US20140234957A1 Engineered Cellular Pathways for Programmed Autoregulation of Differentiation 有权
标题翻译：用于区分程序自动调节的工程细胞途径
公开(公告)号：US20140234957A1
公开(公告)日：2014-08-21
申请号：US14174475
申请日：2014-02-06
申请人： The Trustees of Princeton University
发明人： Ron Weiss , Ihor Lemischka , Priscilla Purnick
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , C12N2800/40 , C12N2830/002 , C12N2830/003 , C12N2830/005 , C12N2830/006 , C12N2830/008 , C12N2830/15 , C12N2830/55 , C12N2830/85
摘要： The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.
摘要翻译：本发明提供用于编程哺乳动物细胞以实现所需功能的组合物和方法。特别地，本发明提供了用于编程干细胞以分化成所需细胞类型的组合物和方法。调节细胞命运调节因子表达的群体感应系统被引入哺乳动物宿主细胞，例如干细胞。群体感测系统通常包括表达细菌群体感知途径的组分的载体，包括催化自身诱导剂的合成的蛋白质和编码自身诱导剂的调节配偶体的基因以及编码细胞命运调节物的基因可操作地连接的载体由自动诱导剂/调节配体复合物诱导的启动子。该系统还可以包含载体，其中编码另外的细胞命运调节子的基因可操作地连接到由响应于第一分化阶段合成的因子诱导的启动子，从而触发第二阶段的分化。

2. 发明授权

US09458471B2 Engineered cellular pathways for programmed autoregulation of differentiation 有权
标题翻译：用于程序自动调节分化的工程化细胞途径
公开(公告)号：US09458471B2
公开(公告)日：2016-10-04
申请号：US14174475
申请日：2014-02-06
申请人： The Trustees of Princeton University
发明人： Ron Weiss , Ihor Lemischka , Priscilla Purnick , Christoph Schaniel , Miles Miller , Patrick Guye
IPC分类号： C12N15/00 , C12N15/11 , C12N15/09 , C12N15/85
CPC分类号： C12N15/85 , C12N2800/40 , C12N2830/002 , C12N2830/003 , C12N2830/005 , C12N2830/006 , C12N2830/008 , C12N2830/15 , C12N2830/55 , C12N2830/85
摘要： The present invention provides compositions and methods for programming mammalian cells to perform desired functions. In particular, the present invention provides compositions and methods for programming stem cells to differentiate into a desired cell type. A quorum sensing systems that regulates the expression of cell fate regulators is introduced into mammalian host cells, such as stem cells. The quorum sensing systems generally comprises vectors that express the components of a bacterial quorum sensing pathway, including proteins which catalyze the synthesis of an autoinducer and a gene encoding a regulatory partner of the autoinducer, and vectors in which genes encoding cell fate regulators are operably linked to a promoter induced by the autoinducer/regulatory partner complex. The system can also comprise vectors in which genes encoding additional cell fate regulators are operably linked to a promoter that is induced by a factor synthesized in response to a first stage of differentiation, so that a second stage of differentiation is triggered.
摘要翻译：本发明提供用于编程哺乳动物细胞以实现所需功能的组合物和方法。特别地，本发明提供了用于编程干细胞以分化成所需细胞类型的组合物和方法。调节细胞命运调节因子表达的群体感应系统被引入哺乳动物宿主细胞，例如干细胞。群体感测系统通常包括表达细菌群体感知途径的组分的载体，包括催化自身诱导剂的合成的蛋白质和编码自身诱导剂的调节配偶体的基因以及编码细胞命运调节物的基因可操作地连接的载体由自动诱导剂/调节配体复合物诱导的启动子。该系统还可以包含载体，其中编码另外的细胞命运调节子的基因可操作地连接到由响应于第一分化阶段合成的因子诱导的启动子，从而触发第二阶段的分化。

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