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91. 发明申请

US20150216961A1 ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
标题翻译：在植物中的旋风样颗粒生产
公开(公告)号：US20150216961A1
公开(公告)日：2015-08-06
申请号：US14398650
申请日：2013-05-10
申请人： MEDICAGO INC. , MITSUBISHI TANABE PHARMA CORPORATION
发明人： Marc-Andre D'Aoust , Nathalie Landry , Pierre-Olivier Lavoie , Masaaki Arai , Naomi Asahara , David Levi Rutendo Mutepfa , Inga Isabel Hitzeroth , Edward Peter Rybicki
IPC分类号： A61K39/15 , C07K14/005 , C12N7/00
CPC分类号： A61K39/15 , A61K39/12 , A61K2039/5258 , C07K14/005 , C12N7/00 , C12N7/02 , C12N15/8203 , C12N15/8214 , C12N15/8216 , C12N15/8218 , C12N15/8221 , C12N15/8258 , C12N2720/12334 , C12N2720/12351
摘要： A method of producing a virus-like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein for example but not limited to rotavirus protein VP2. The nucleotide sequence may further comprise one or more than one amplification element and/or a compartment targeting sequence. A second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP6. Optionally, a third nucleic acid and/or fourth nucleic acid might be introduced into the plant, or portion of the plant. The third nucleic acid comprises a third regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP4. The fourth nucleic acid comprises a fourth regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP7. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.
摘要翻译：提供了一种在植物中生产病毒样颗粒（VLP）的方法。该方法包括将第一核酸引入植物或植物的一部分。所述第一核酸包含在植物中活性的第一调节区，其可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP2）的核苷酸序列。核苷酸序列还可以包含一个或多于一个扩增元件和/或隔室靶向序列。可以将第二种核酸引入植物或植物的一部分。第二核酸包含在植物中有活性的第二调控区，并可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP6）的核苷酸序列。任选地，第三核酸和/或第四核酸可以被引入植物或植物的一部分。第三核酸包含在植物中活性的第三调节区，并且与编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP4）的核苷酸序列可操作地连接。第四核酸包含在植物中活性的第四调节区，并且可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP7）的核苷酸序列。将植物或植物的一部分在允许核酸表达的条件下温育，从而产生VLP。

92. 发明申请

US20150101081A1 PLANT TRANSFORMANT, TRANSFORMATION METHOD FOR PLANT, AND VECTOR USED IN SAID METHOD 审中-公开
标题翻译：植物变异体，植物转化方法和用于确定方法的矢量
公开(公告)号：US20150101081A1
公开(公告)日：2015-04-09
申请号：US14360379
申请日：2012-11-22
申请人： National Institute of Agrobiological Sciences
发明人： Yutaka Tabei , Ayako Okuzaki
IPC分类号： C12N15/82
CPC分类号： C12N15/8238 , C12N15/8214
摘要： The present invention provides a method for transforming organelles having an own genomic DNA, the method enabling efficient production of highly homoplasmic plant cells, in which most of the organelles are transformed, and so forth. The method comprises the steps of: expressing in plant cells a fusion protein containing a function inhibiting factor of the organelles and a transit signal peptide to the organelles; introducing into the genomic DNA of the organelles of the plant cells an expression cassette comprising DNAs encoding a restoring factor of the organelles and a factor desired to be expressed in the organelles; and allowing the function inhibiting factor to destroy the organelles, in which the expression cassette is not introduced, in the plant cells.
摘要翻译：本发明提供了一种用于转化具有自身基因组DNA的细胞器的方法，该方法能够高效地产生大多数细胞器被转化的高度均质的植物细胞，等等。该方法包括以下步骤：在植物细胞中表达含有细胞器的功能抑制因子和转运信号肽至融合细胞器的融合蛋白; 向植物细胞的细胞器的基因组DNA中引入包含编码细胞器的恢复因子和期望在细胞器中表达的因子的DNA的表达盒; 并且允许功能抑制因子在植物细胞中破坏其中未引入表达盒的细胞器。

93. 发明申请

US20150096082A1 Expression of the Human IGF-1 In Transgenic Plastids 审中-公开
标题翻译：人IGF-1在转基因质体中的表达
公开(公告)号：US20150096082A1
公开(公告)日：2015-04-02
申请号：US14553647
申请日：2014-11-25
申请人： UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.
发明人： Henry Daniell
IPC分类号： C07K14/65 , C12N15/82
CPC分类号： C07K14/65 , A61K38/00 , C12N15/8214 , C12N15/8257
摘要： A plastid transformation vector for stably transforming a plastid genome, comprising, as operably-linked components, a first flanking sequence, a DNA sequence coding for synthetic insulin-like growth factor-1 (IGF-1s) (SEQ ID NO. 1) or a substantially homologous DNA sequence of IGF-1s, which is capable of expression in the plastid genome, and a second flanking sequence.
摘要翻译：用于稳定转化质体基因组的质体转化载体，其包含作为可操作连接的组分的第一侧翼序列，编码合成的胰岛素样生长因子-1（IGF-1）（SEQ ID NO.1）的DNA序列或能够在质体基因组中表达的IGF-1的基本上同源的DNA序列和第二侧翼序列。

94. 发明申请

US20150047074A1 NANOBIONIC ENGINEERING OF ORGANELLES AND PHOTOSYNTHETIC ORGANISMS 审中-公开
标题翻译：有机物和光合作用的纳米工程
公开(公告)号：US20150047074A1
公开(公告)日：2015-02-12
申请号：US14454196
申请日：2014-08-07
申请人： Michael S. Strano , Juan Pablo Giraldo Gomez , Sean Mitchell Faltermeier , Markita P. Landry
发明人： Michael S. Strano , Juan Pablo Giraldo Gomez , Sean Mitchell Faltermeier , Markita P. Landry
IPC分类号： C12N5/04 , A01H5/00
CPC分类号： C12N5/04 , A01H3/00 , A01H5/00 , C12N15/8207 , C12N15/8212 , C12N15/8214 , C12N15/8269 , C12N15/87 , G01N33/52
摘要： In one aspect, a composition can include an organelle, and a nanoparticle having a zeta potential of less than −10 mV or greater than 10 mV contained within the organelle. In a preferred embodiment, the organelle can be a chloroplast and the nanoparticle can be a single-walled carbon nanotube associated with a strongly anionic or strongly cationic polymer.
摘要翻译：一方面，组合物可以包含细胞器和细胞器内含有小于-10mV或大于10mV的ζ电位的纳米颗粒。在优选的实施方案中，细胞器可以是叶绿体，并且纳米颗粒可以是与强阴离子或强阳离子聚合物相关联的单壁碳纳米管。

95. 发明授权

US08932576B2 Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast 有权
标题翻译：生物活性哺乳动物蛋白在衣藻叶绿体中的稳健表达
公开(公告)号：US08932576B2
公开(公告)日：2015-01-13
申请号：US12695101
申请日：2010-01-27
申请人： Stephen P. Mayfield
发明人： Stephen P. Mayfield
IPC分类号： A61K48/00 , C12P21/06 , C12N1/12 , C12N15/82
CPC分类号： C12N15/79 , C12N15/8214 , C12N15/8257
摘要： Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.
摘要翻译：公开的方法和组合物通过直接替代叶绿体光系统II（PSII）反应中心蛋白编码区来构建包含异源哺乳动物基因的叶绿体，以实现高于总蛋白质5％以上的重组蛋白的表达。当使用藻类时，藻类表达的蛋白质主要产生为可溶性蛋白质，其中肽的功能活性是完整的。由于宿主藻类是可食用的，所以公开了在该生物体中生产用于口服递送蛋白质/肽，特别是肠道活性蛋白质而不进行纯化的生物制剂。

96. 发明申请

US20140219971A1 ALGAL PRODUCED MALARIAL TRANSMISSION BLOCKING VACCINES 有权
标题翻译： ALGAL生产的疟疾传播阻塞疫苗
公开(公告)号：US20140219971A1
公开(公告)日：2014-08-07
申请号：US14123519
申请日：2012-05-01
申请人： Stephen P. Mayfield , James A. Gregory , Carla S. Jones , Michael J. Hannon
发明人： Stephen P. Mayfield , James A. Gregory , Carla S. Jones , Michael J. Hannon
IPC分类号： C07K14/445
CPC分类号： C07K14/445 , A61K39/015 , A61K2039/517 , A61K2039/6037 , C12N1/12 , C12N15/8214 , C12N15/8258 , Y02A50/412
摘要： The present invention relates to the production of malaria transmission blocking vaccines in single-celled green algae, particularly algae of the genus Chlamydomonas, e.g., Chlamydomonas reinhardtii; the immunogenic Plasmodium polypeptides produced and compositions comprising them; and methods for preventing, ameliorating, reducing, delaying, treating and blocking the transmission of malaria by administration of immunogenic Plasmodium polypeptides produced in an algal host cell.
摘要翻译：本发明涉及在单细胞绿藻，特别是衣藻属例如莱茵衣藻的藻类中生产疟疾传播阻断疫苗; 产生的免疫原性疟原虫多肽和包含它们的组合物; 以及通过施用在藻类宿主细胞中产生的免疫原性疟原虫多肽来预防，改善，减少，延迟，治疗和阻断疟疾传播的方法。

97. 发明申请

US20140201864A1 Polynucleotides and Polypeptides in Plants 审中-公开
标题翻译：植物中的多核苷酸和多肽
公开(公告)号：US20140201864A1
公开(公告)日：2014-07-17
申请号：US14177551
申请日：2014-02-11
申请人： Mendel Biotechnology, Inc.
发明人： Roderick W. Kumimoto , Luc J. Adam , Roger Canales , Karen S. Century , Robert A. Creelman , Jennifer M. Costa , Neal I. Gutterson , Frederick D. Hempel , Jacqueline E. Heard , Cai-Zhong Jiang , Katherine Krolikowski , Omaira Pineda , Emily L. Queen Kumimoto , Oliver J. Ratcliffe , Peter P. Repetti , T. Lynne Reuber , Jose Luis Riechmann , James Z. Zhang
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8214 , C12N15/8245 , C12N15/8247 , C12N15/8249 , C12N15/825 , C12N15/8251 , C12N15/8261 , C12N15/8262 , C12N15/8266 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8287 , C12N15/8291 , C12N15/8293 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法，并且也被公开。

98. 发明申请

US20140075592A1 Intercellular Transfer of Organelles in Plants for Horizontal Transfer of DNA Expressing Proteins of Interest 审中-公开
标题翻译：细胞器在细胞器中的水平转移表达蛋白质的植物的细胞间的转移
公开(公告)号：US20140075592A1
公开(公告)日：2014-03-13
申请号：US13930378
申请日：2013-06-28
申请人： RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
发明人： Pal Maliga , Zora S. Maliga , Gregory N. Thyssen
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , A01H1/00 , C12N15/8202
摘要： Compositions and methods for effecting horizontal gene transfer in plants are disclosed.
摘要翻译：公开了在植物中进行水平基因转移的组合物和方法。

99. 发明申请

US20140066606A1 PRODUCTION OF HIGH MANNOSE GLYCOSYLATED PROTEINS STORED IN THE PLASTID OF MICROALGAE 审中-公开
标题翻译：生产存在于微藻中的高甘露糖苷化蛋白
公开(公告)号：US20140066606A1
公开(公告)日：2014-03-06
申请号：US13997670
申请日：2011-12-28
申请人： Aude Carlier , Remy Michel , Jean-Paul Cadoret , Alexandre Lejeune , Nathalie Dufourmantel
发明人： Aude Carlier , Remy Michel , Jean-Paul Cadoret , Alexandre Lejeune , Nathalie Dufourmantel
IPC分类号： C12N15/82 , C07K14/505 , C12Q1/34 , C07K14/005 , C12P21/00 , G01N33/68
CPC分类号： C07K14/005 , C07K14/505 , C12N9/2402 , C12N15/8214 , C12N15/8257 , C12N15/8258 , C12N2740/16122 , C12P21/005 , C12Q1/34 , C12Y302/01045 , G01N33/6842
摘要： The present invention concerns a transformed microalga producing a protein harboring a “high mannose” pattern of glycosylation in the plastid of the transformed microalga, wherein 1) the transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) the microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, the nucleic acid sequence encoding an amino acid sequence including (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of the protein, 3) the xylosyltransferases and fucosyltransferases of the microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of the microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of the microalga have not been inactivated.
摘要翻译：本发明涉及转化的微藻，其在转化微藻的质体中产生具有糖基化的“高甘露糖”模式的蛋白质，其中1）转化的微藻具有叶绿体内质网（CER）; 2）微藻已经用可操作地连接到启动子的核酸序列转化，编码氨基酸序列的核酸序列包括（i）由至少一个信号肽组成的氨基末端二分子发射信号（BTS）序列，其依次是通过转运肽; 和（ii）蛋白质的序列，3）微藻的木糖基转移酶和岩藻糖基转移酶尚未失活; 4）微藻的N-乙酰糖基转移酶I未被灭活，优选微藻的N-乙酰基糖基转移酶II，III，IV，V和VI，甘露糖苷酶II和糖基转移酶未被灭活。

100. 发明申请

US20130203132A1 MANIPULATION OF GENES OF THE MEVALONATE AND ISOPRENOID PATHWAYS TO CREATE NOVEL TRAITS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：操纵梅毒基因和异常路径在转基因生物体系中创造新的条件
公开(公告)号：US20130203132A1
公开(公告)日：2013-08-08
申请号：US13602723
申请日：2012-09-04
申请人： Frederick M. Hahn , Adelheid R. Kuehnle
发明人： Frederick M. Hahn , Adelheid R. Kuehnle
IPC分类号： C12P9/00
CPC分类号： C12P9/00 , C12N9/90 , C12N15/52 , C12N15/8209 , C12N15/8214 , C12N15/8243
摘要： Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).
摘要翻译：公开了甲羟戊酸和类异戊二烯生物合成途径的特定基因和无活性基因位点（假基因）的用途，以（1）增强转基因植物和微藻质体中异戊烯基二磷酸，二甲基烯丙基二磷酸和异戊二烯通路衍生产物的生物合成，（2）创建新的抗生素抗性转基因植物和微藻，（3）创建一个新的选择系统和/或靶向位点，用于介导遗传物质插入植物和微藻质体。待使用的单独或以任何组合形式存在的特异性多核苷酸可从GeneBank公开获得并含有具有序列的开放阅读框，其表达将产生具有以下酶活性的活性蛋白：（a）乙酰乙酰辅酶A硫解酶（EC 2.3.1.9 ），（b）3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）合酶（EC 4.1.3.5），（c）HMG-CoA还原酶（EC 1.1.1.34），（d）甲羟戊酸激酶（e）磷酸戊糖酸盐激酶（EC 2.7.4.2），（f）甲羟戊酸二磷酸脱羧酶（EC 4.1.1.33），（g）异戊烯基二磷酸（IPP）异构酶（EC 5.3.3.2）和（h）合成酶（EC 2.5.1.32）。

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