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91. 发明申请

US20120102982A1 METHOD AND SYSTEM FOR NUCLEATION CONTROL IN A CONTROLLED RATE FREEZER (CRF) 有权
标题翻译：控制速度冻结机（CRF）中核心控制的方法与系统
公开(公告)号：US20120102982A1
公开(公告)日：2012-05-03
申请号：US13097724
申请日：2011-04-29
申请人： Ying Zhou , Theodore H. Gasteyer, III , Nigel J. Grinter , Alan T. Cheng , Yeu-Chuan Simon Ho , Robert R. Sever
发明人： Ying Zhou , Theodore H. Gasteyer, III , Nigel J. Grinter , Alan T. Cheng , Yeu-Chuan Simon Ho , Robert R. Sever
IPC分类号： F25D17/04 , F25D31/00
CPC分类号： A01N1/0289 , A01N1/0257 , A01N1/0284 , F25D3/102 , F25D29/001
摘要： A method and system for controlled rate freezing and nucleation of materials is provided. The presently disclosed system and method provides the ability to rapidly cool the materials contained in vials or other containers within a cooling unit via forced convective cooling using a laminar and uniform flow of cryogen in proximity to the plurality of vials disposed within the cooling unit. The rapid cooling of the biological materials is achieved by precisely controlling and adjusting the temperature of the cryogen being introduced to the system as a function of time. The presently disclosed methods to systems also provide nucleation control via temperature quench and depressurized nucleation control.
摘要翻译：提供了控制速率冷冻和成核的方法和系统。本公开的系统和方法提供了通过强制对流冷却来快速冷却包含在冷却单元内的小瓶或其他容器中的材料的能力，其中使用层流和均匀流动的冷冻剂靠近设置在冷却单元内的多个小瓶。生物材料的快速冷却是通过精确地控制和调节作为时间的函数引入到系统中的冷冻剂的温度来实现的。目前公开的系统方法还通过温度骤冷和减压成核控制提供成核控制。

92. 发明申请

US20110217723A1 EPI-ILLUMINATION APPARATUS AND METHODS FOR SORTING PARTICLES 有权
标题翻译： EPI-照明装置和用于分选颗粒的方法
公开(公告)号：US20110217723A1
公开(公告)日：2011-09-08
申请号：US13106705
申请日：2011-05-12
申请人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
发明人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
IPC分类号： C12Q1/02 , C12M1/34
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A method and apparatus for sorting stained particles in a fluid stream, each of the method and apparatus including an epi-illumination optics system with a focusing lens. The optics system being operable to direct a beam of electromagnetic radiation through the focusing lens in a forward direction along a beam axis intersecting particles in the fluid stream at said first location so that said particles pass through the beam, resulting in the fluorescence emissions from the particles being directed along said beam axis in a rearward direction.
摘要翻译：一种用于在流体流中分选染色颗粒的方法和装置，所述方法和装置中的每一个包括具有聚焦透镜的外延照明光学系统。所述光学系统可操作以沿着与所述第一位置处的所述流体流中的颗粒相交的束轴在向前方向上引导电磁辐射束通过所述聚焦透镜，使得所述颗粒通过所述光束，导致来自所述光束的荧光发射颗粒沿着所述光束轴线沿向后方向被引导。

93. 发明申请

US20100311036A1 Methods for Augmentation of Cell Cryopreservation 审中-公开
标题翻译：增强细胞冷冻保存的方法
公开(公告)号：US20100311036A1
公开(公告)日：2010-12-09
申请号：US12796994
申请日：2010-06-09
申请人： Xiaoming He
发明人： Xiaoming He
IPC分类号： C12N5/02
CPC分类号： A01N1/0284
摘要： In accordance with certain embodiments of the present disclosure, a method for cryopreserving a cell is described. The method includes encapsulating a cell in a microcapsule, the microcapsule having a diameter of less than about 100 μM. The method further includes vitrifying the encapsulated cell in a vitrifying solution comprising a cryoprotectant, wherein the cell is cooled at a rate of equal to or greater than 30,000° C./min and the cryoprotectant is present at a concentration of less than or equal to 1.5 M.
摘要翻译：根据本公开的某些实施方案，描述了一种用于冷冻保存细胞的方法。该方法包括将细胞包封在微胶囊中，该微胶囊具有小于约100μM的直径。该方法还包括将包封的细胞玻璃化在包含冷冻保护剂的玻璃化溶液中，其中细胞以等于或大于30,000℃/分钟的速率冷却，并且冷冻保护剂以小于或等于 1.5 M.

94. 发明申请

US20100018862A1 CELLOMICS SYSTEM 审中-公开
标题翻译：细胞系统
公开(公告)号：US20100018862A1
公开(公告)日：2010-01-28
申请号：US12471993
申请日：2009-05-26
申请人： Kazunori OKANO , Kenji Yasuda
发明人： Kazunori OKANO , Kenji Yasuda
IPC分类号： B01D57/02
CPC分类号： B01L7/50 , A01N1/0284 , G01N33/5061 , G01N33/566
摘要： In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.
摘要翻译：在标记细胞并根据使用细胞分离器的标记程度分离和收集细胞时，对细胞的影响被最小化，并且促进使用所收集的细胞，从而当标记细胞时，细胞被标记在保持每个单元的相互作用的状态下。在标记中，存在于靶细胞表面上的特定标记材料经由转运体取出，细胞逐个分散，将其与细胞分离器分离。分离后立即将细胞置于不含特定标记物质的溶液中以除去细胞中所含的特异性标记物质。该系列步骤用细胞分离芯片连续进行。

95. 发明申请

US20100016568A1 CELLOMICS SYSTEM 审中-公开
公开(公告)号：US20100016568A1
公开(公告)日：2010-01-21
申请号：US12471947
申请日：2009-05-26
申请人： Kazunori Okano , Kenji Yasuda
发明人： Kazunori Okano , Kenji Yasuda
IPC分类号： C40B40/08 , C07H21/04
CPC分类号： B01L7/50 , A01N1/0284 , G01N33/5061 , G01N33/566
摘要： In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

96. 发明申请

US20090258337A1 Thawed organ or tissue or thawed cell group to be donated, transplanted, added, or administered to living body, production process thereof, supercooled solution therefor, and production apparatus of the organ or tissue 审中-公开
标题翻译：解冻的器官或组织或解冻的细胞组被给予，移植，添加或给予生物体，其生产方法，过冷溶液和器官或组织的生产装置
公开(公告)号：US20090258337A1
公开(公告)日：2009-10-15
申请号：US12383582
申请日：2009-03-25
申请人： Shunichi Yagi
发明人： Shunichi Yagi
IPC分类号： A01N1/02 , C12N5/06 , C12N5/08 , F25B21/00
CPC分类号： A01N1/0284 , A01N1/0221 , A01N1/0226
摘要： The present invention aims to freeze and store organs or tissues taken from human bodies or animals and after thawing, use the resulting organs or tissues, or cells taken and extracted therefrom for medical treatment. The present invention is characterized in that the organs or tissues taken from human bodies or animals are regenerated through the steps of freezing, storing and thawing; the cells taken from the organs or tissues undergo cell division and colonization.
摘要翻译：本发明的目的在于冷冻和储存从人体或动物取出的器官或组织，并在解冻之后，使用得到的器官或组织，或从其取出和提取的细胞进行治疗。本发明的特征在于，从人体或动物取出的器官或组织通过冷冻，储存和解冻的步骤再生; 从器官或组织取出的细胞经历细胞分裂和定植。

97. 发明申请

US20090176271A1 Systems for Efficient Staining and Sorting of Populations of Cells 有权
标题翻译：高效染色和分选细胞群的系统
公开(公告)号：US20090176271A1
公开(公告)日：2009-07-09
申请号：US12404931
申请日：2009-03-16
申请人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
发明人： Gary Durack , Jeffrey D. Wallace , Gary P. Vandre , Lon A. Westfall , Jeremy T. Hatcher , Niraj V. Nayak
IPC分类号： G01N1/30
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.
摘要翻译：一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。集成平台可以包括电磁辐射的共同来源。本发明的方法包括使用共享整合平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

98. 发明申请

US20080050717A1 Cryopreservation and recovery system for liquid substances 审中-公开
标题翻译：液体物质的冷冻保存和回收系统
公开(公告)号：US20080050717A1
公开(公告)日：2008-02-28
申请号：US11881561
申请日：2007-07-27
申请人： William Brower , Louis Bigelow , David Schedgick
发明人： William Brower , Louis Bigelow , David Schedgick
IPC分类号： A01N1/02
CPC分类号： A01N1/0284 , A01N1/02
摘要： The present invention provides a method of preserving human red blood cells including the acts of cryogenically freezing a number of human red blood cells suspended in a fluid at a rate of 20° C. to 100° C. per second, maintaining a predetermined thickness of the fluid between a pair of plates during freezing of the plurality of red blood cells, and warming the cryogenically frozen red blood cells to an ambient temperature to recover at least some of the red blood cells.
摘要翻译：本发明提供了一种保存人类红细胞的方法，包括以每秒20℃至100℃的速率将悬浮在流体中的许多人红细胞进行低温冻结的作用，保持预定厚度的在多个红细胞冷冻期间在一对板之间的流体，以及将低温冷冻的红细胞升温至环境温度以回收至少一些红细胞。

99. 发明申请

US20060063141A1 Method of cryopreserving cells 审中-公开
标题翻译：冷冻保存细胞的方法
公开(公告)号：US20060063141A1
公开(公告)日：2006-03-23
申请号：US11228388
申请日：2005-09-19
申请人： Locksley McGann , Janet Elliott , Lisa Ross-Rodriguez
发明人： Locksley McGann , Janet Elliott , Lisa Ross-Rodriguez
IPC分类号： A01N1/02 , G06F19/00
CPC分类号： A01N1/02 , A01N1/0284
摘要： A non-linear cooling cryopreservation method for improving cryopreservation protocols for cells that involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells using simulation of cellular responses and experimental results; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cooling cryopreservation protocol for cryopreserving stem cells is also disclosed that does not require cryoprotectants.
摘要翻译：用于改善细胞冷冻保存方案的非线性冷却冷冻保存方法，其涉及产生对一系列冷却参数的细胞反应的模拟; 确定使用细胞反应和实验结果的模拟来最小化对细胞的冷冻损伤所需的最佳冷却参数; 并将最佳参数并入协议。该模拟基于细胞参数的数学模型。还公开了用于冷冻保存干细胞的非线性冷却冷冻保存方案，其不需要冷冻保护剂。

100. 发明申请

US20050112541A1 Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm 有权
标题翻译：用于分选颗粒和提供性别分类的动物精子的装置，方法和方法
公开(公告)号：US20050112541A1
公开(公告)日：2005-05-26
申请号：US10812351
申请日：2004-03-29
申请人： Gary Durack , Jeffrey Wallace , Gary Vandre , Lon Westfall , Jeremy Hatcher , Niraj Nayak
发明人： Gary Durack , Jeffrey Wallace , Gary Vandre , Lon Westfall , Jeremy Hatcher , Niraj Nayak
IPC分类号： A01N1/02 , C12M1/34 , C12N5/071 , C12Q1/04 , G01N1/30 , G01N15/00 , G01N15/14 , G01N33/48 , G01N33/487 , G01N33/50
CPC分类号： C12N5/0612 , A01N1/0284 , C12N5/06 , C12N5/061 , C12Q1/02 , C12Q3/00 , G01N15/1468 , G01N15/147 , G01N21/63 , G01N21/6428 , G01N33/48 , G01N33/5005 , G01N2015/0065 , G01N2015/1006 , G01N2015/149 , G01N2021/6439 , Y10T436/10 , Y10T436/115831 , Y10T436/12 , Y10T436/25 , Y10T436/2525 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.
摘要翻译：一种用于根据一种或多种颗粒特性分级颗粒的多通道装置。该装置包括多个流式细胞术单元，每个流式细胞术单元可操作以通过用电磁辐射束询问含有颗粒的流体流来分类颗粒混合物中的颗粒。流式细胞术单元共享包括以下至少一个的综合平台：（1）通常的颗粒供应; （2）共同住房; （3）用于控制单元的操作的通用处理器; （4）用于从各单元接收和处理信息的通用处理器; 和（5）普通流体输送系统。集成平台可以包括电磁辐射的共同来源。本发明的方法包括使用共享该集成平台的多个流式细胞仪单元来进行诸如分析或分选颗粒的流量计操作。

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