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1. 发明授权

US07820402B2 Immunoassay element 失效
标题翻译：免疫测定元件
公开(公告)号：US07820402B2
公开(公告)日：2010-10-26
申请号：US11230127
申请日：2005-09-19
申请人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
发明人： Yoshiki Sakaino , Hitomi Ito , Toshihiro Mori , Osamu Seshimoto , Toshihisa Ito , Yoshikazu Amano
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/54386 , G01N2333/924 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/807 , Y10S436/81
摘要： The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.
摘要翻译：用于通过确定由免疫反应引起的酶标记的抗原或抗体的酶活性的变化来定量分析抗原的免疫测定元件。免疫测定元件包括含有在标记酶存在下形成可扩散材料的不扩散基底的底物层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。作为非扩散性底物，使用能够仅与标记酶反应并且不能使片段化酶反应的底物。当使用内源活性葡糖苷酶作为标记酶，并且使用外源活性葡糖苷酶时，试剂层中的断裂酶，底物层的非扩散性基质优选为内型选择性反应性底物，其中是指具有对内源性葡糖苷酶特异性的反应性的底物。高精度，高重现性和良好的储存稳定性实现了高灵敏度测定。

2. 发明授权

US06979543B2 Method for detecting biological agents 失效
标题翻译：检测生物制剂的方法
公开(公告)号：US06979543B2
公开(公告)日：2005-12-27
申请号：US10412568
申请日：2003-04-10
申请人： Liaohai Chen , Duncan W. McBranch , Hsing-Lin Wang , David G. Whitten
发明人： Liaohai Chen , Duncan W. McBranch , Hsing-Lin Wang , David G. Whitten
IPC分类号： C07C319/14 , C07C323/12 , G01N33/543 , C12Q1/68
CPC分类号： G01N33/54373 , C07B2200/05 , C07C319/14 , G01N33/5438 , G01N33/54386 , Y10S435/808 , Y10S435/966 , Y10S436/805 , Y10S436/806 , Y10S436/808 , Y10S436/823 , Y10S530/811 , Y10S530/812 , C07C323/12
摘要： A sensor is provided including a polymer capable of having an alterable measurable property from the group of luminescence and electrical conductivity, the polymer having an intermediate combination of a recognition element, a tethering element and a property-altering element bound thereto and capable of altering the measurable property, the intermediate combination adapted for subsequent separation from the polymer upon exposure to an agent having an affinity for binding to the recognition element whereupon the separation of the intermediate combination from the polymer results in a detectable change in the alterable measurable property, and, detecting said detectable change in the alterable measurable property.
摘要翻译：提供了一种传感器，其包括能够从发光和导电性组中具有可改变的可测量性质的聚合物，所述聚合物具有识别元件，束缚元件和结合到其上的改变属性的元件的中间组合，并且能够改变可测量的性质，适于在暴露于具有与识别元件结合的亲和力的试剂之后随后与聚合物分离的中间体组合，因此中间体组合与聚合物的分离导致可改变的可测量性质的可检测变化，检测可变的可测量性质中的所述可检测变化。

3. 发明授权

US06589731B1 Method for detecting biological agents 失效
标题翻译：检测生物制剂的方法
公开(公告)号：US06589731B1
公开(公告)日：2003-07-08
申请号：US09565589
申请日：2000-05-04
申请人： Liaohai Chen , Duncan W. McBranch , Hsing-Lin Wang , David G. Whitten
发明人： Liaohai Chen , Duncan W. McBranch , Hsing-Lin Wang , David G. Whitten
IPC分类号： G01N3353
CPC分类号： G01N33/5438 , G01N33/542 , G01N33/54373 , G01N33/54386 , Y10S435/808 , Y10S435/966 , Y10S436/805 , Y10S436/806 , Y10S436/808 , Y10S436/823
摘要： A sensor element is provided including a polymer exhibiting a measurable property from the group of luminescence and electrical conductivity, the polymer being complexed with a unit including a recognition element, a tethering element and a property-altering element bound thereto so as to alter the measurable property, the unit being susceptible of subsequent separation from the polymer upon exposure to an agent having an affinity for binding to the recognition element whereupon the separation of the unit from the polymer results in a detectable change in the measurable property.
摘要翻译：提供了一种传感器元件，其包括从发光和导电性组中显示可测量性质的聚合物，所述聚合物与包括识别元件，系链元件和结合到其上的改变属性的单元复合，以便改变可测量的性质，该单元易于暴露于具有与识别元件结合的亲和性的试剂后随后与聚合物分离，因此单元与聚合物的分离导致可测量性质的可检测变化。

4. 发明申请

US20030124622A1 Procedure for the determination of the activity of the protease which activates factor VII from protein solutions 审中-公开
公开(公告)号：US20030124622A1
公开(公告)日：2003-07-03
申请号：US10287047
申请日：2002-11-04
发明人： Juergen Roemisch , Annette Feussner , Hans-Arnold Stoehr
IPC分类号： G01N033/573
CPC分类号： C12Q1/56 , G01N33/573 , G01N33/86 , G01N2333/96447 , G01N2800/324 , G01N2800/368 , Y10S435/966 , Y10S436/811
摘要： A procedure for the determination of the activity of the protease which activates blood clotting factor VII from protein solutions is describes, in which the protein solution comprising the protease and/or its proenzyme is incubated with a solid phase to which an antibody which binds the protease and/or its proenzyme has been coupled beforehand, and after washing the solid phase, the protease and/or its proenzyme fixed thereto, are incubated with reagents which allow for the determination of their activity.

5. 发明授权

US06448029B1 Method and reagent for quantitative determination of 1,5-anhydroglucitol 有权
标题翻译：用于定量测定1,5-脱水葡萄糖醇的方法和试剂
公开(公告)号：US06448029B1
公开(公告)日：2002-09-10
申请号：US09929038
申请日：2001-08-15
申请人： Sakae Tazoe , Akira Miike
发明人： Sakae Tazoe , Akira Miike
IPC分类号： C12Q132
CPC分类号： C12Q1/32 , C12Q1/54 , Y10S435/966 , Y10S435/975
摘要： The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要翻译：本发明提供了用于确定特定成分的简单方法，例如，含有葡萄糖的样品中的1,5-脱水葡萄糖醇（1,5-AG），以及可用于该方法的试剂和试剂盒。在一个实施方案中，提供了用于测定1,5-AG的方法，其包括将样品与将葡萄糖转化为果糖-1,6-二磷酸并将1,5-AG转化为1,5-AG的酶系统接触 -6-磷酸形成1,5-AG-6-磷酸，通过1,5-AG-6-磷酸脱氢酶的作用在氧化型辅酶存在下使样品中的1,5-AG-6-磷酸脱氢，并测定由脱氢反应形成的还原型辅酶的量。还提供了一种在该方法中有用的试剂和试剂盒。

6. 发明申请

US20020119500A1 Multiplexed enzymatic assays 失效
标题翻译：多重酶检测
公开(公告)号：US20020119500A1
公开(公告)日：2002-08-29
申请号：US09924692
申请日：2001-08-08
发明人： Qifeng Xue , Ian Gibbons
IPC分类号： G01N033/53 , G01N033/542 , C12N009/00 , C12N009/12
CPC分类号： G01N33/561 , C12Q1/00 , C12Q1/485 , G01N2333/9121 , G01N2333/91215 , G01N2500/04 , G01N2500/20 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A mutiplexed enzyme assay method and substrate set for performing the method are disclosed. The method includes performing a plurality of enzyme reactions in the presence of a plurality of enzymes substrates, under conditions effective to convert an enzyme substrate to a corresponding product, where the product of each substrate and the substrate have different separation characteristics from each other and from the other substrates and their corresponding products. After performing the reactions, which may be carried out in separate or combined reactions, the substrates and products in said reactions are separated in a single separation medium. For each separated product and substrate, a separation characteristic effective to identify that product and substrate and a signal related to the amount of the product and substrate is detected. From this, one can determine the amount of substrate converted to the corresponding product in each of the reactions.
摘要翻译：公开了用于执行该方法的复合酶测定方法和底物组。该方法包括在有效将酶底物转化成相应产物的条件下，在多个酶底物的存在下进行多个酶反应，其中每个底物和底物的产物彼此具有不同的分离特性其他基材及其相应的产品。在进行可以在单独或组合反应中进行的反应之后，所述反应中的底物和产物在单一分离介质中分离。对于每个分离的产物和底物，检测到有效地鉴定该产物和底物的分离特性和与产物和底物的量相关的信号。由此，可以确定在每个反应中转化成相应产物的底物的量。

7. 发明授权

US06287767B1 Chemiluminescent energy transfer assays 有权
标题翻译：化学发光能量转移测定
公开(公告)号：US06287767B1
公开(公告)日：2001-09-11
申请号：US09190181
申请日：1998-11-12
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N3353
CPC分类号： G01N33/581 , C07D321/00 , C12Q1/34 , G01N33/535 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assays for the determination of the presence or amount of a biopolymer in bound assays using 1,2-dioxetanes in connection with AttoPhos™ as chemiluminescent substrates for enzyme-labeled targets or probes is provided. Further disclosed is a kit for conducting a bioassay for the presence or concentration of a biopolymer comprising a) an enzyme complex; b) a 1,2-dioxetane; and c) AttoPhos™.
摘要翻译：提供了一种化学发光测定法，用于在结合测定中测定生物聚合物的存在或数量，使用与作为酶标记的靶标或探针的化学发光底物的AttoPhos TM相关的1,2-二氧杂环己烷。还公开了用于进行生物聚合物的存在或浓缩的试剂盒，其包含a）酶复合物; b）1,2-二氧环乙烷; 和c）AttoPhos TM。

8. 发明授权

US06015683A Dry analytical element for acetaminophen assay 失效
标题翻译：对乙酰氨基酚测定用干燥分析元素
公开(公告)号：US06015683A
公开(公告)日：2000-01-18
申请号：US914915
申请日：1992-07-15
申请人： James Robert Schaeffer , John Charles Mauck , Robert Francis Winterkorn , Thomas Charles Arter
发明人： James Robert Schaeffer , John Charles Mauck , Robert Francis Winterkorn , Thomas Charles Arter
IPC分类号： C12Q1/00 , C12Q1/34 , C12Q1/37 , C12Q1/26 , C12Q1/28
CPC分类号： C12Q1/34 , G01N2333/98 , Y10S435/966 , Y10S435/969 , Y10S435/97 , Y10S436/815
摘要： A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
摘要翻译：用于检测水性流体中对乙酰氨基酚的分光光度测定法可以用干燥的分析元件进行。元件包括其上具有一个或多个试剂层的载体，其含有第一种酶，芳基酰胺酶，以切割对乙酰氨基酚的酰胺键以产生对氨基苯酚; 第二种酶，抗坏血酸氧化酶，以氧化对氨基苯酚，使其与水溶性偶联剂偶联形成在670nm读取的染料。该测定法在血清和血浆样品中精确，准确，并且相对没有显着的干扰。该元件还允许在宽动态范围内进行测量。

9. 发明授权

US5998155A Stable composition of immobilized protein having affinity for biotin 失效
标题翻译：具有对生物素亲和力的固定化蛋白质的稳定组成
公开(公告)号：US5998155A
公开(公告)日：1999-12-07
申请号：US904368
申请日：1997-08-01
申请人： Steven James Burton , James C. Pearson , Peter A. D. Edwardson
发明人： Steven James Burton , James C. Pearson , Peter A. D. Edwardson
IPC分类号： C12N11/10 , A61L2/00 , A61L24/10 , C07K1/22 , C07K14/36 , C07K14/465 , C07K14/75 , C07K17/10 , C12N11/16 , C12P21/06 , C07D235/02 , C12N11/00 , G01N33/569
CPC分类号： C07K14/36 , A61L2/0035 , A61L24/106 , C07K1/22 , C07K14/465 , C07K14/75 , C07K17/10 , C12P21/06 , Y10S435/966 , Y10S435/967 , Y10S435/972 , Y10S530/81 , Y10S530/812 , Y10S530/813
摘要： Compositions and methods for avidin or protein having affinity for biotin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels for binding biotin and may include a bulking agent, e.g., maltose, and a protectant to maintain the stability and integrity of the composition during lyophilization and terminal sterilization processes. The compositions have applicability in any instance where avidin agarose and/or the avidin/biotin technology are useful. In particular, the present compositions are useful in an enzyme capture system to prepare fibrin monomers useful for fibrin sealants.
摘要翻译：公开了对固定在惰性载体材料例如琼脂糖上的生物素具有亲和性的抗生物素蛋白或蛋白质的组合物和方法。组合物具有高的结合生物素的活性水平，并且可以包括填充剂，例如麦芽糖，以及在冻干和终末灭菌过程中保持组合物的稳定性和完整性的保护剂。组合物在抗生物素蛋白琼脂糖和/或抗生物素蛋白/生物素技术是有用的任何情况下都具有适用性。特别地，本发明的组合物可用于酶捕获系统中以制备可用于纤维蛋白密封剂的纤维蛋白单体。

10. 发明授权

US5792617A Cell proliferation-based amplified detection of analytes 失效
标题翻译：基于细胞增殖的分析物扩增检测
公开(公告)号：US5792617A
公开(公告)日：1998-08-11
申请号：US476229
申请日：1995-06-07
申请人： M. Boris Rotman
发明人： M. Boris Rotman
IPC分类号： G01N33/532 , C12Q1/02 , C12Q1/18 , C12Q1/68 , G01N33/53 , G01N33/569 , G01N33/574 , G01N33/58
CPC分类号： C12Q1/18 , G01N33/5308 , G01N33/56966 , G01N33/56972 , G01N33/57484 , G01N33/57492 , G01N33/58 , G01N33/581 , Y10S435/834 , Y10S435/839 , Y10S435/848 , Y10S435/878 , Y10S435/881 , Y10S435/883 , Y10S435/966 , Y10S436/807 , Y10S436/808 , Y10S436/813
摘要： A test kit and method for the highly sensitive detection of specific analytes in a sample is provided. The presence of the analyte in the sample results in a decrease in the concentration of a growth inhibiting substance leading to proliferation of cells in the region of the analyte. The presence or absence of the analyte is determined by detecting the presence of increased numbers of cells. Assay sensitivity is accounted for by the exponential amplification of cell number that occurs during cell proliferation in the presence of analyte.
摘要翻译：提供了一种用于样品中特异性分析物的高灵敏度检测的测试试剂盒和方法。样品中分析物的存在导致生长抑制物质的浓度降低，导致分析物区域中的细胞增殖。分析物的存在或不存在通过检测增加数目的细胞的存在来确定。在分析物存在下细胞增殖期间细胞数量的指数扩增可以解释测定灵敏度。

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