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1. 发明授权

US07709230B2 Process for producing isomaltose and uses thereof 有权
标题翻译：异麦芽糖的制备方法及其用途
公开(公告)号：US07709230B2
公开(公告)日：2010-05-04
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/44 , C12P19/18 , C12P19/04 , C12N9/00 , C12N9/10 , C12P21/06 , C07H1/00
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种生产异麦芽糖的新方法及其应用，包括以下步骤：将非还原末端具有至少两个葡萄糖聚合度和至少两个α-1,4糖苷键作为键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基葡糖，其在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原端以外的键，和/或形成环{→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （ 1→6）-α-D-吡喃葡萄糖基 - （1→3）-α-D-吡喃葡萄糖基 - （1→};使与异麦芽糖释放酶形成的糖接触释放异麦芽糖;收集释放的异麦芽糖; 其中。

2. 发明申请

US20080241904A1 PROCESS FOR PRODUCING ISOMALTOSE AND USES THEREOF 有权
标题翻译：生产异构体的方法及其用途
公开(公告)号：US20080241904A1
公开(公告)日：2008-10-02
申请号：US11777044
申请日：2007-07-12
申请人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
发明人： Michio Kubota , Tomoyuki Nishimoto , Takanobu Higashiyama , Hikaru Watanabe , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10
CPC分类号： C12P19/12
摘要： A novel process for producing isomaltose and uses thereof and comprising the steps of contacting saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, with an α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme to form a-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1″3)-α-D-glucopyranosyl-(1→}; contacting the saccharides so formed with isomaltose-releasing enzyme to release isomaltose; and collecting the released isomaltose; and uses thereof.
摘要翻译：一种制备异麦芽糖的新方法及其用途，包括以下步骤：将具有至少两个葡萄糖聚合度的至少两个和非1,4-还原末端的α-1,4糖苷键的糖与α-异麦芽糖基葡萄糖在α-异麦芽糖基转移酶的存在或不存在下形成α-异麦芽糖基糖，其具有在非还原末端具有至少三个α-1,6糖苷键作为键的葡萄糖聚合度和α-1,4糖苷键作为非还原末端之外的键，和/或形成环{ - > 6）-al-D-glucopyranosyl-（1-> 3）-al-D-glucopyranosyl - （1-> 6）-al-D-glucopyranosyl-（1''3）-alpha-D-吡喃葡萄糖基 - （1->};使所形成的糖与异麦芽糖释放酶接触以释放异麦芽糖; 释放的异麦芽糖;及其用途。

3. 发明授权

US07732426B2 Isocyclomaltooligosaccharide (S) , isocyclomaltooligosaccharide-forming enzyme, their preparation and uses 有权
标题翻译：异环寡糖（S），异环寡糖寡糖形成酶，其制备和用途
公开(公告)号：US07732426B2
公开(公告)日：2010-06-08
申请号：US11663919
申请日：2005-09-26
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , A61K31/702 , C07H1/00
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide(s) or a saccharide composition comprising the same, and uses thereof. Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→} General Formula 1 (In General Formula 1, “n” means a number of 4 or 5).
摘要翻译：本发明的目的是提供一种由葡萄糖组成的新型非还原性糖组成的非还原性糖类，提供形成非还原性糖类的新型酶，其制造方法及其制造方法，编码酶的DNA，重组DNA和包含DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有由通式1表示的结构的异环型低聚半乳糖，新型的异环型低聚葡萄糖形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体来解决上述目的包含DNA，包含异环寡脱醇糖的组合物或包含该组合物的糖组合物及其用途。环状{→6） - [α-D-Glcp-（1→4）] n-α-D-Glcp-（1→}通式1（在通式1中，“n”表示4或5 ）。

4. 发明授权

US07309697B2 Cyclic maltosylmatose 有权
标题翻译：循环麦芽糖
公开(公告)号：US07309697B2
公开(公告)日：2007-12-18
申请号：US10569959
申请日：2004-08-26
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K31/715 , C07H1/00
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{ - > 6）-α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6） - 结构的新型环状糖来解决上述目的。 α-D-吡喃葡萄糖基 - （1-> 4）-α-D-吡喃葡萄糖基 - （1-> 6），环状麦芽糖基麦芽糖，新型环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码酶，重组DNA和包含该DNA的转化体，包含环状麦芽糖基麦芽糖的组合物或包含其的糖组合物及其用途。

5. 发明申请

US20100285562A1 ISOCYCLOMALTOOLIGOSACCHARIDE(S), ISOCYCLOMALTOOLIGOSACCHARIDE-FORMING ENZYME, THEIR PREPARATION AND USES 有权
标题翻译：异羟肟酸（SOCYCLOMALTOOLIGOSACCHARIDE）（S），异羟肟酸吡喃葡萄糖苷形成酶，其制备和用途
公开(公告)号：US20100285562A1
公开(公告)日：2010-11-11
申请号：US12764927
申请日：2010-04-21
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/00 , C12N1/20 , C07H21/04 , C12N15/70 , C12N1/21
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}, wherein “n” is 4 or 5.
摘要翻译：通过提供由葡萄糖组成的新型非还原性糖类，形成非还原性糖类的新型酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体，得到非还原性糖类，该DNA是含有非还原性糖的组合物及其用途，通过使用具有式1所示结构的环状环糊精寡糖（Cyclo（→6）） - [α-D-Glcp-（1→4））] n-α-D-Glcp-（1→}，其中“n”为4或5。

6. 发明申请

US20100015671A1 CYCLIC MALTOSYLMALTOSE, CYCLIC MALTOSYMALTOSE-FORMING ENZYME, THEIR PREPARATION AND USES 有权
标题翻译：环状麦芽糖醇，环状麦芽糖醇形成酶，其制备和用途
公开(公告)号：US20100015671A1
公开(公告)日：2010-01-21
申请号：US11941862
申请日：2007-11-16
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/18 , C12N9/10
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{> 6）-α-D-吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1,6）-alpha-D结构的新型环状糖来解决上述目的 - 吡喃葡萄糖基 - （1,4）-alpha-D-吡喃葡萄糖基 - （1>），环状麦芽糖基麦芽糖，新的环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体包含该DNA，包含环状麦芽糖麦芽糖的组合物或包含其的糖组合物及其用途。

7. 发明授权

US08633005B2 Isocyclomaltooligosaccharide(s), isocyclomaltooligosaccharide-forming enzyme, their preparation and uses 有权
标题翻译：异环寡糖，异环寡糖寡糖形成酶，其制备和应用
公开(公告)号：US08633005B2
公开(公告)日：2014-01-21
申请号：US12764927
申请日：2010-04-21
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12N9/10 , C12N15/54
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： A non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents, a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof are provided by use of an isocyclomaltooligosaccharide(s) having a structure represented by Formula 1, Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}, wherein “n” is 4 or 5.
摘要翻译：通过提供由葡萄糖组成的新型非还原性糖类，形成非还原性糖类的新型酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体，得到非还原性糖类， DNA，包含非还原性糖的组合物及其用途通过使用具有由式1表示的结构的环状小麦寡糖，环 - { - 6） - [α-D-Glcp-（1- > 4）] n-α-D-Glcp-（1->}，其中“n”为4或5。

8. 发明授权

US07951530B2 Cyclic maltosylmaltose, cyclic maltosymaltose-forming enzyme, their preparation and uses 有权
标题翻译：循环麦芽糖苷，环状麦芽糖基酶形成酶，其制备和用途
公开(公告)号：US07951530B2
公开(公告)日：2011-05-31
申请号：US11941862
申请日：2007-11-16
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： G01N33/573 , C12Q1/48 , C12P1/00 , C13K7/00 , C07H1/00
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.
摘要翻译：本发明的目的是通过提供一种由葡萄糖组成的新型非还原性糖组成并提供形成非还原性糖的新型酶，提供一种非还原性糖的方法及其制备方法，编码该酶的DNA，重组DNA和包含该DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有环{（6）-α-D-吡喃葡萄糖基 - （1,4）-α-D-吡喃葡萄糖基 - （1,6）-α-D结构的新型环状糖来解决上述目的 - 吡喃葡萄糖基 - （1> 4）-α-D-吡喃葡萄糖基 - （1>），环状麦芽糖基麦芽糖，新型环状麦芽糖基麦芽糖酶形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体包含该DNA，包含环状麦芽糖麦芽糖的组合物或包含其的糖组合物及其用途。

9. 发明申请

US20080131468A1 Isocyclomaltooligosaccharide (S) , Isocyclomaltooligosaccharide-Forming Enzyme, Their Preparation and Uses 有权
标题翻译：异环寡糖（S），异环寡糖 - 形成酶，其制备及用途
公开(公告)号：US20080131468A1
公开(公告)日：2008-06-05
申请号：US11663919
申请日：2005-09-26
申请人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： A61K8/02 , C13K5/00 , C12N9/00 , C12N1/20 , A61K31/715 , A23L2/38 , A23G3/00 , C07H21/04 , C12N1/00 , C12P19/12
CPC分类号： C07H3/06 , A23L29/35 , A23L33/25 , C12N9/2402 , C12N9/2405 , C12P19/04
摘要： The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide (s) or a saccharide composition comprising the same, and uses thereof. Cyclo{→6)-[α-D-Glcp-(1→4)]n-α-D-Glcp-(1→}General Formula 1 (In General Formula 1, “n” means a number of 4 or 5)
摘要翻译：本发明的目的是提供一种由葡萄糖组成的新型非还原性糖组成的非还原性糖类，提供形成非还原性糖类的新型酶，其制造方法及其制造方法，编码酶的DNA，重组DNA和包含DNA的转化体，包含非还原性糖的组合物及其用途。本发明通过提供具有由通式1表示的结构的异环型低聚半乳糖，新型的异环型低聚葡萄糖形成酶，其制备方法和方法，编码该酶的DNA，重组DNA和转化体来解决上述目的包含DNA，包含异环寡脱醇糖的组合物或包含该组合物的糖组合物及其用途。 <？in-line-formula description =“In-line Formulas”end =“lead”？> Cyclo { - > 6） - [alpha-D-Glcp-（1→4）] }通式1 <？in-line-formula description =“In-line Formulas”end =“tail”？>（在通用公式1中，“n” 4或5）

10. 发明申请

US20070066565A1 Cyclic maltosylmatose 有权
公开(公告)号：US20070066565A1
公开(公告)日：2007-03-22
申请号：US10569959
申请日：2004-08-26
申请人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
发明人： Kazuhisa Mukai , Hikaru Watanabe , Tomoyuki Nishimoto , Michio Kubota , Shigeharu Fukuda , Toshio Miyake
IPC分类号： C12P19/04 , C07H21/04 , A61K31/715 , C12P21/06 , C12N9/10
CPC分类号： A61K9/2018 , A21D2/181 , A23L2/52 , A23L2/60 , A23L13/428 , A23L27/33 , A23L33/10 , A61K8/60 , A61Q11/00 , C07H3/06 , C12N9/1048 , C12P19/18
摘要： An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→4)-α-D-glucopyranosyl-(1→6}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

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