会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 10. 发明公开
    • 글루탐산을 생산하는 코리네형 세균을 이용한 폴리감마 글루탐산의 제조방법
    • 使用生产CORYNE-BACTERIA的谷氨酸生产聚谷氨酸的方法
    • KR1020050025824A
    • 2005-03-14
    • KR1020030062761
    • 2003-09-08
    • 주식회사 바이오리더스
    • 성문희박청김광석박제현최윤호김형범홍승표이일한정창민
    • C12N1/21
    • C12N15/74C12P13/14C12R1/15
    • A method for producing poly-gamma-glutamate using a glutamate producing Coryne-type microorganism is provided, thereby reducing the production costs by adding no glutamate into a medium, producing large molecular weight of poly-gamma-glutamate by using Coryne-type microorganism having no enzyme decomposing poly-gamma-glutamate, and easily recovering poly-gamma-glutamate from the cultured Coryne-type microorganism that is easily recovered from the medium. A transformed Coryne-type microorganism with a vector pEC99-HCE-pgsBCA comprising genes encoding enzymes producing poly-gamma-glutamate is provided, wherein the gene encoding the enzyme producing poly-gamma-glutamate is isolated from Bacillus subtilis chungkookjang(KTCC 0697BP); and the genes encoding the enzymes producing poly-gamma-glutamate are pgsB, pgsC and pgsA; the Coryne-type microorganism is Corynebacterium sp. or Brevibacterium sp.; and the Corynebacterium sp. is Corynebacterium glutamicum(ATCC 13032). The method for producing poly-gamma-glutamate comprises culturing a glutamate producing Coryne-type microorganism in a medium without glutamate.
    • 提供了使用谷氨酸生产棒状微生物生产聚-γ-谷氨酸的方法,由此通过在培养基中不添加谷氨酸来降低生产成本,通过使用具有谷氨酸的棒状微生物产生大分子量的聚-γ-谷氨酸 没有酶分解聚-γ-谷氨酸盐,并且容易地从容易从培养基中回收的培养的棒状细菌中回收聚-γ-谷氨酸。 提供了包含编码产生多聚-γ-谷氨酸的酶的基因的载体pEC99-HCE-pgsBCA的转化棒状型微生物,其中编码产生多聚谷氨酸的酶的基因从枯草芽孢杆菌chungkookjang(KTCC 0697BP)分离; 并且编码产生多聚-γ-谷氨酸的酶的基因是pgsB,pgsC和pgsA; 棒状微生物是棒状杆菌属。 或短杆菌属。 和棒状杆菌属 是谷氨酸棒杆菌(ATCC 13032)。 制备聚-γ-谷氨酸的方法包括在不含谷氨酸的培养基中培养产生谷氨酸的棒状细菌。