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    • 4. 发明授权
    • 신규 알카리성 리파제, 이를 생산하는 신규 바실러스 균주 및 리파제 대량생산 방법
    • 신규알카리성리파제,이를생산하산신규바실러스균주및리파제대량생산방
    • KR100463350B1
    • 2004-12-29
    • KR1020010061667
    • 2001-10-06
    • 한국생명공학연구원주식회사 바이오리더스
    • 성문희오태광김형권최윤호김광석김영선김철중송재준김광배진우신광순부하령이상철
    • C12N9/14
    • PURPOSE: Provided are a novel alkaline lipase, a novel bacillus sp. strain producing the same, and a method of producing the lipase massively. The non-toxicity bacillus sp. strain is capable of being used in food industry, and the lipase is used for quantitative determination of lipid content. CONSTITUTION: The novel alkaline lipase shows thermostability at 40 deg.C and has an optimum temperature of activity at 35 deg.C. Further, it has no changes with calcium concentrations, and has an optimum activity at pH 8.5. Its amino acid sequence is represented by the SEQ ID NO:1. The novel bacillus sp. strain, bacillus pumilus B26 strain(KTCC 10081BP) produces the lipase. The lipase is mass-produced by transforming bacillus subtilis DB104 with a recombinant vector pMB26 then culturing the transformant. The transformant strain is bacillus subtilis DB104/pMB26(KCTC 10082BP).
    • 目的:提供了一种新型碱性脂肪酶,一种新型的芽孢杆菌。 产生它的菌株,以及大量生产脂肪酶的方法。 无毒芽孢杆菌 菌株能够在食品工业中使用,并且脂肪酶用于定量测定脂质含量。 构成:新型碱性脂肪酶在40摄氏度显示热稳定性,在35摄氏度时具有最佳活性温度。 此外,钙浓度没有变化,并且在pH 8.5时具有最佳活性。 其氨基酸序列由SEQ ID NO:1表示。 新型芽孢杆菌 菌株,短小芽孢杆菌B26菌株(KTCC10081BP)产生脂肪酶。 通过用重组载体pMB26转化枯草芽孢杆菌DB104然后培养转化体来大量生产脂肪酶。 转化株是枯草芽孢杆菌DB104 / pMB26(KCTC10082BP)。
    • 8. 发明公开
    • 고분자량의 폴리-감마-글루탐산을 생산하는 내염성 균주 바실러스 서브틸리스 청국장
    • 生产高分子量聚乳酸的BSA-4能够生产聚氨基酸的高分子量的HALOPHILE MICROORGANISM BACILLUS SUBTILIS BS-4
    • KR1020020079889A
    • 2002-10-19
    • KR1020027010976
    • 2001-08-11
    • 한국생명공학연구원주식회사 바이오리더스
    • 성문희백대헌이승구송재준홍승표최윤호한재숙아시우치마코토소다겐지부하령나유진
    • C12N1/20
    • C12R1/125C07K14/32
    • PURPOSE: Provided are halophile microorganism Bacillus subtilis BS-4 capable of producing high molecular weight of poly-γ-glutamic acid and a method for producing the high molecular weight of poly-γ-glutamic acid using the same, thereby producing the high molecular weight poly-γ-glutamic acid in higher yield. CONSTITUTION: The halophile microorganism Bacillus subtilis BS-4 (KCTC 0697BP) capable of producing high molecular weight of poly-γ-glutamic acid is isolated from 'Chug Kook Jang'(Fermented soybean paste) by the steps of: diluting 'Chug Kook Jang' in distilled water; heating the diluted solution at 60 deg. C for 20 minutes; plating the diluted solution on GS agar medium; culturing it at 37 deg. C for 3 days; isolating high viscosity of colonies; culturing the colonies in a broth at 37 deg. C for 20 hours; and selecting a strain showing the best growth activity. The method for producing the high molecular weight of poly-γ-glutamic acid comprises the steps of: (a) culturing the Bacillus subtilis BS-4(KCTC 0697BP) to obtain poly-γ-glutamic acid; and (b) removing polysaccharides from the poly-γ-glutamic acid; dissolving the precipitate of poly-γ -glutamic acid and adding a protease into the solution to decompose an extracellular protein; and dialyzing the solution to remove free glutamic acid and concentrating the solution.
    • 目的:提供能够生产高分子量聚-γ-谷氨酸的嗜碱性微生物枯草芽孢杆菌BS-4,以及使用其制备高分子量聚-γ-谷氨酸的方法,从而产生高分子量 聚-γ-谷氨酸。 构成:通过以下步骤从“Chug Kook Jang”(发酵大豆酱)分离能够产生高分子量聚-γ-谷氨酸的嗜碱性微生物枯草芽孢杆菌BS-4(KCTC 0697BP),稀释“Chug Kook Jang 在蒸馏水中 将稀释溶液加热至60℃。 C 20分钟; 将稀释的溶液电镀在GS琼脂培养基上; 在37度培养 C 3天; 分离菌落的高粘度; 在37℃的肉汤培养菌落。 C 20小时; 并选择显示最佳生长活性的菌株。 制备高分子量聚-γ-谷氨酸的方法包括以下步骤:(a)培养枯草芽孢杆菌BS-4(KCTC 0697BP)以获得聚-γ-谷氨酸; 和(b)从聚-γ-谷氨酸中除去多糖; 溶解聚-γ-谷氨酸的沉淀物,并向溶液中加入蛋白酶以分解细胞外蛋白质; 并透析溶液以除去游离谷氨酸并浓缩溶液。