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    • 6. 发明公开
    • 바실러스의 고농도 배양을 위한 배지 조성물 및 이의 용도
    • 用于高浓度培养基的培养基组合物及其用途
    • KR1020120076873A
    • 2012-07-10
    • KR1020100138621
    • 2010-12-30
    • 대한민국(농촌진흥청장)
    • 김동운김영화조성백정현정이성대김상호조규호황옥화김인철김수기
    • C12N1/20C12N1/38C12R1/07
    • PURPOSE: A medium composition containing corn starch, soybean powder, and yeast extract is provided to cheaply produce Bacillus at high productivity. CONSTITUTION: A medium composition for producing Bacillus of high concentration contains 15-25 g/L of corn starch, 4-6 g/L of soybean powder, and 4-6 g/L of yeast extract. The medium composition additionally contains 0.1-1 g/L of dipotassium phosphate(K_2HPO_4), 0.01-0.1 g/L of magnesium chloride(MgCl_2), 0.01-0.1 g/L of calcium chloride(CaCl_2), and 0.1-1 g/L of sodium chloride(NaCl). The Bacillus includes Bacillus subtilis or Bacillus licheniformis. A method for producing Bacillus of high concentration comprises a step of inoculating Bacillus to the medium composition and culturing.
    • 目的:提供含有玉米淀粉,大豆粉和酵母提取物的培养基组合物以高生产率便宜地生产芽孢杆菌。 构成:用于生产高浓度芽胞杆菌的培养基组合物含有15-25g / L的玉米淀粉,4-6g / L的大豆粉和4-6g / L的酵母提取物。 该培养基组合物另外含有0.1-1g / L的磷酸氢二钾(K_2HPO_4),0.01-0.1g / L的氯化镁(MgCl 2),0.01-0.1g / L的氯化钙(CaCl 2)和0.1-1g / L氯化钠(NaCl)。 芽孢杆菌包括枯草芽孢杆菌或地衣芽孢杆菌。 生产高浓度芽孢杆菌的方法包括将芽孢杆菌接种到培养基组合物并培养的步骤。