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    • 2. 发明公开
    • 올리고뉴클레오티드를 이용한 차량 식별 표지 및 차량감식 방법
    • 使用寡核苷酸和寡核苷酸标记识别汽车的方法
    • KR1020040033799A
    • 2004-04-28
    • KR1020020063057
    • 2002-10-16
    • (주)바이오니아
    • 박한오길준모이상주이재돈김영희
    • C12Q1/68
    • C07H21/00C09D7/00C12Q1/68C12Q2563/185Y02P20/55
    • PURPOSE: A method for identifying a car using oligonucleotides and an oligonucleotide marker used therein are provided, thereby easily identifying a car by sequencing oligonucleotides contained in paint pieces of the car. CONSTITUTION: An oligonucleotide marker for identifying a car consists of an oligonucleotide coupled with a phase transfer agent(PTA), wherein the oligonucleotide contains the coding sequence and the primer sequence for polymerase chain reaction(PCR) positioned in its both ends; and the coding sequence consists of 10 to 50 base pairs. A method for labeling a car with the oligonucleotide comprises the steps of: (1) coupling the phase transfer agent(PTA) with the oligonucleotide having the coding sequence in an organic solvent; (2) binding a reaction-blocking protecting group with the PTA-coupled oligonucleotide; adding the oligonucleotide into a coating material for car; and (4) coating the car with the car coating material. a method for identifying a car using the oligonucleotide comprises the steps of: (1) extracting the oligonucleotide marker from samples of a car; (2) removing a reaction-blocking protecting group from the oligonucleotide marker; (3) sequencing the oligonucleotide; and (4) identifying the car using the oligonucleotide sequence.
    • 目的:提供使用寡核苷酸鉴定车辆的方法及其中使用的寡核苷酸标记,从而通过对包含在轿厢的油漆块中的寡核苷酸进行测序来容易地识别轿厢。 构成:用于鉴定汽车的寡核苷酸标记物由与相转移剂(PTA)偶联的寡核苷酸组成,其中寡核苷酸包含位于其两端的编码序列和聚合酶链式反应(PCR)引物序列; 编码序列由10到50个碱基对组成。 用寡核苷酸标记汽车的方法包括以下步骤:(1)将相转移剂(PTA)与编码序列的寡核苷酸偶联在有机溶剂中; (2)与PTA偶联寡核苷酸结合反应阻断保护基; 将寡核苷酸加入到汽车涂料中; 和(4)用汽车涂料涂漆汽车。 使用寡核苷酸鉴定汽车的方法包括以下步骤:(1)从汽车的样品中提取寡核苷酸标记; (2)从寡核苷酸标记中除去反应阻断保护基; (3)测序寡核苷酸; 和(4)使用寡核苷酸序列识别汽车。