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1. 发明专利

JP2008048736A Method for separating microorganism from sample by using electrodialysis and means for capturing microorganism, and microorganism-separating device therefor 有权
标题翻译：通过电化学分析方法分离微生物的方法和用于捕获微生物的方法及其微生物分离装置
公开(公告)号：JP2008048736A
公开(公告)日：2008-03-06
申请号：JP2007214950
申请日：2007-08-21
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： HAN JUNG-IM , KIM JOON-HO , JEONG SUNG-YOUNG , LEE YOUNG-SUN , HWANG KYU-YOUN , LEE HUN-JOO
IPC分类号： C12Q1/02 , C12M1/00 , C12N15/09 , C12Q1/68
CPC分类号： C12N1/02 , B01D61/48
摘要： PROBLEM TO BE SOLVED: To provide a method for separating microorganisms from samples by using electrodialysis and means for capturing microorganisms, and a microorganism-separating device therefor. SOLUTION: Disclosed is a method for separating microorganisms from samples which are biological samples, wherein the method comprises steps of: introducing samples containing microorganisms into a reaction chamber of a salt concentration-regulating device providing a reaction chamber having end portions separated by a cation-exchange membrane and an anion-exchange membrane, a first electrode chamber comprising an end portion separated by the anion-exchange membrane and a first electrode and filled with an ion exchange medium and a second electrode chamber comprising an end portion separated by the cation-exchange membrane and a second electrode and filled with an ion exchange medium; lowering the salt concentration of the sample containing microorganisms by electrodialyzing the samples containing the microorganisms in the reaction chamber through applying voltage between the first electrode and the second electrode; and bringing the sample having a reduced salt concentration into contact with the means for capturing microorganisms. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供通过使用电渗析和微生物捕获装置从样品中分离微生物的方法及其微生物分离装置。解决方案：公开了一种从作为生物样品的样品中分离微生物的方法，其中所述方法包括以下步骤：将含有微生物的样品引入到盐浓度调节装置的反应室中，所述盐浓度调节装置提供具有由阳离子交换膜和阴离子交换膜，第一电极室，包括由阴离子交换膜分离的端部和第一电极，并填充有离子交换介质，第二电极室包括由阳离子交换膜和第二电极，并填充有离子交换介质; 通过在第一电极和第二电极之间施加电压，通过在反应室中电渗析含有微生物的样品来降低含有微生物的样品的盐浓度; 并使具有降低的盐浓度的样品与用于捕获微生物的装置接触。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2007306914A Method for carrying out the concentration and the amplification of nucleic acid in single microchamber and apparatus for carrying out the same 审中-公开
标题翻译：在单个微波炉中实现核酸浓度和核酸浓度的方法及其实施方法
公开(公告)号：JP2007306914A
公开(公告)日：2007-11-29
申请号：JP2007085582
申请日：2007-03-28
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： KIM YOUNG-ROCK , MIN JUN-HONG , LEE IN-HO , LEE YOUNG-SUN , YOO CHANG-EUN , HAN KI-WOONG
IPC分类号： C12N15/09 , C12M1/00
CPC分类号： C12Q1/6806 , B01L3/5027 , B01L3/5088 , C12Q1/686 , C12Q2565/629 , C12Q2537/149 , C12Q2527/125
摘要： PROBLEM TO BE SOLVED: To provide a method for carrying out the concentration and the amplification of nucleic acids in a single microchamber and an apparatus for carrying out the same. SOLUTION: A nucleic acid-containing specimen and a solution including kosmotropic salt are introduced into a microchamber having the hydrophilic inside surface to bond the nucleic acid on the inner surface, thereby enabling the step for concentration of the nucleic acids and the step for polymerase chain reaction (PCR) be continuously carried out when the mixture for PCR is continuously added in the single microchamber. Further, the apparatus for carrying out the method is also provide. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供在单个微室中进行核酸的浓缩和扩增的方法及其实施的装置。解决方案：将含核酸的样品和包含差异性盐的溶液引入到具有亲水性内表面的微室中以将核酸粘合在内表面上，从而使得能够使核酸浓缩的步骤和步骤当在单个微室中连续添加用于PCR的混合物时，连续进行聚合酶链反应（PCR）。此外，还提供了用于执行该方法的装置。版权所有（C）2008，JPO＆INPIT

3. 发明专利

JP2006337363A Salt concentration regulator, lab-on-chip provided therewith, and salt concentration regulating method using the same 审中-公开
标题翻译：盐浓度调节剂，其中提供的LAB-芯片和使用其的盐浓度调节方法
公开(公告)号：JP2006337363A
公开(公告)日：2006-12-14
申请号：JP2006145650
申请日：2006-05-25
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： HAN JUNG-IM , LEE YOUNG-SUN , KIM JOON-HO
IPC分类号： G01N35/08 , C12M1/00 , C12N15/09 , G01N27/327 , G01N27/416 , G01N37/00
CPC分类号： B01D61/422 , B01D61/46 , B01L3/5027 , G01N2001/4011
摘要： PROBLEM TO BE SOLVED: To provide a salt concentration regulator capable of regulating a salt concentration in-situ. SOLUTION: This salt concentration regulator is provided with (a) a reaction chamber defined by a cation-exchange membrane and an anion-exchange membrane, and selected from a group comprising a biomolecule extraction chamber, an amplifying chamber, a hybridization chamber and a detection chamber, (b) a primary electrode chamber defined by the anion-exchange membrane and a primary electrode, and containing an ion-exchange medium, and (c) a secondary electrode chamber defined by the cation-exchange membrane and a secondary electrode, and containing the ion-exchange medium. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供能够原位调节盐浓度的盐浓度调节剂。解决方案：该盐浓度调节器具有（a）由阳离子交换膜和阴离子交换膜限定的反应室，并且选自包括生物分子提取室，放大室，杂交室和检测室，（b）由阴离子交换膜和初级电极限定并含有离子交换介质的主电极室，（c）由阳离子交换膜和次级隔膜限定的二次电极室电极，并含有离子交换介质。版权所有（C）2007，JPO＆INPIT

4. 发明专利

JP2006068011A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2006068011A
公开(公告)日：2006-03-16
申请号：JP2005246323
申请日：2005-08-26
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: This method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme, and then calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding background fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：用于从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或扩增对应于不包括背景荧光信号的最大荧光信号的强度的一半，对应于最大扩增效率的放大循环次数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2009106298A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2009106298A
公开(公告)日：2009-05-21
申请号：JP2008313681
申请日：2008-12-09
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises: a step of amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme; a step of generating a function representing correlation with strength of fluorescent signal by amplification amount shown in real time by amplification cycle number or amplification time of the nucleic acid; a step of calculating a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals of the nucleic acid by utilizing the function; and a step of obtaining initial concentration of the nucleic acid from the calculated unamplified fluorescent light signal strength. Thereby, the early concentration of the nucleic acid can be determined without using a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括：用酶扩增从生物体或病毒提取的核酸（DNA或RNA）的步骤; 通过扩增循环次数或核酸扩增时间实时显示荧光信号强度的相关性函数的步骤; 通过利用该功能计算除了核酸的背景荧光信号之外的未扩增核酸荧光信号的强度的步骤; 以及从计算的未扩增的荧光信号强度获得核酸的初始浓度的步骤。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2009072206A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2009072206A
公开(公告)日：2009-04-09
申请号：JP2008313682
申请日：2008-12-09
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data includes amplifying a nucleic acids (DNA or RNA) extracted from an organism or virus with an enzyme, and then, calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding back ground fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或对应于不包括背景荧光信号的最大荧光信号的强度的一半的放大时间，对应于最大扩增效率的扩增循环数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2008197097A Centrifugal force based microflow apparatus for dilution and microflow system comprising it 有权
标题翻译：用于渗透和微流控系统的基于离心力的微流体装置
公开(公告)号：JP2008197097A
公开(公告)日：2008-08-28
申请号：JP2008026810
申请日：2008-02-06
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： CHO YOON-KYOUNG , LEE JEONG-GUN , LEE BEOM SEOK , PARK SHOBEN , LEE YOUNG-SUN
IPC分类号： B01L99/00 , G01N35/00 , B01F11/00 , G01N35/08 , G01N37/00
CPC分类号： B01L3/502738 , B01L3/50273 , B01L3/502792 , B01L2200/0605 , B01L2200/0647 , B01L2200/16 , B01L2300/0806 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/1861 , B01L2400/0409 , B01L2400/0677
摘要： PROBLEM TO BE SOLVED: To provide a centrifugal force based microflow apparatus for dilution and a microflow system comprising it. SOLUTION: This apparatus includes: rotatable disc type platform 10; a mixing chamber 50 arranged on the platform 10; a buffer liquid preservation part which is arranged nearer to the mixing chamber 50 than to the center of the platform 10, is connected with the mixing chamber 50 through a channel, and supplies a buffer liquid of at least constant quantity each time to the mixing chamber 50; and a plurality of dilution chambers 61-67 which are arranged farther from the center of the platform 10 than the mixing chamber 50, each of which is connected to the mixing chamber 50 through a channel extending from a middle outlet corresponding to a fixed water level of the mixing chamber 50, and which sequentially receive the dilution which has been diluted in the mixing chamber 50 at least once. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于稀释的基于离心力的微流装置和包括其的微流系统。解决方案：该装置包括：旋转盘式平台10; 布置在平台10上的混合室50; 配置在比混合室50更靠近平台10的中心的缓冲液保存部通过通道与混合室50连接，并且每次将至少一定量的缓冲液供给混合室 50; 以及多个稀释室61-67，其布置成比混合室50更靠近平台10的中心，每个混合室通过从对应于固定水位的中间出口延伸的通道连接到混合室50 的混合室50，并且其顺序地接收已经在混合室50中稀释的稀释液至少一次。版权所有（C）2008，JPO＆INPIT

8. 发明专利

JP2007275057A Method for concentrating and breaking cell or virus 审中-公开
标题翻译：用于浓缩和破坏细胞或病毒的方法
公开(公告)号：JP2007275057A
公开(公告)日：2007-10-25
申请号：JP2007065329
申请日：2007-03-14
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： CHO YOON-KYOUNG , LEE JEONG-GUN , PARK SHOBEN , LEE YOUNG-SUN , LEE KOEN
IPC分类号： C12N15/09 , C12M1/33 , C12N1/02 , C12N1/06 , C12N7/02
CPC分类号： C12N1/066 , C12M47/06 , C12N13/00 , G01N33/54326
摘要： PROBLEM TO BE SOLVED: To provide a method for concentrating and breaking a cell or a virus.
SOLUTION: The method for concentrating and breaking the cell or the virus comprises a step for pouring beads into a solution containing the cell or the virus and concentrating the cell or the virus on the beads and a step for breaking the cell or the virus by irradiating laser to the beads.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供用于浓缩和破坏细胞或病毒的方法。解决方案：用于浓缩和断裂细胞或病毒的方法包括将珠子倒入含有细胞或病毒的溶液中并将细胞或病毒浓缩在珠上的步骤以及用于破坏细胞或病毒通过向珠子照射激光。版权所有（C）2008，JPO＆INPIT

9. 发明专利

JP2006198614A Handheld centrifuge 审中-公开
标题翻译：手持中心
公开(公告)号：JP2006198614A
公开(公告)日：2006-08-03
申请号：JP2006001840
申请日：2006-01-06
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , LEE YOUNG-SUN , OH KWANG-WOOK , KO CHRISTOPHER HANSUNG , HAN JUNG-IM
IPC分类号： B04B1/00 , A61M1/02 , B04B9/00
CPC分类号： B01L3/5021 , B01L2300/043 , B01L2300/0681 , B04B5/0414 , B04B9/00 , B04B9/12
摘要： PROBLEM TO BE SOLVED: To provide an easily carried handheld centrifuge generating a centrifugal force without using a motor.
SOLUTION: In the handheld centrifuge, an inertia body has an axle to which one end of a string is connected, a pair of inertia wheels coupled to the axle, and at least one sealed vessel is installed at the inertia body to contain a substance to be centrifuged.
COPYRIGHT: (C)2006,JPO&NCIPI

10. 发明专利

JP2005058234A Pcr primer set for detecting hepatitis b, and method for detecting hepatitis b and using the primer set 审中-公开
标题翻译：用于检测乙型肝炎的PCR引物组和用于检测乙型肝炎和使用PRIMER SET的方法
公开(公告)号：JP2005058234A
公开(公告)日：2005-03-10
申请号：JP2004231611
申请日：2004-08-06
申请人： Samsung Electronics Co Ltd , 三星電子株式会社
发明人： LEE YOUNG-SUN , HAN JUNG-IM , HWANG JUNG-JOO , KIM MI-KYUNG , CHO YOON-KYOUNG , LIM HEE-KYUN , KIM YOUNG-A
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/706 , C12Q2565/629 , C12Q2565/543 , C12Q2531/113
摘要： PROBLEM TO BE SOLVED: To provide a PCR primer set for detecting hepatitis B, and to provide a method for detecting hepatitis B and using the primer set. SOLUTION: Provided are a primer set selected from a group consisting of primers having a specific base sequence, a method for detecting hepatitis B and including a step of PCR by using the primer set, and a hepatitis B detection kit including the primer set. Thereby, hepatitis B is quickly and accurately diagnosed. COPYRIGHT: (C)2005,JPO&NCIPI

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