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1. 发明专利

JP2006129869A Nucleic acid purification apparatus and nucleic acid purification method 有权
标题翻译：核酸纯化装置和核酸纯化方法
公开(公告)号：JP2006129869A
公开(公告)日：2006-05-25
申请号：JP2005319853
申请日：2005-11-02
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： LEE JEONG-GUN , KWON YOUNG-NAM , KIM YOUNG-A
IPC分类号： C12N15/09 , C12M1/00
CPC分类号： B01L3/502761 , B01L3/502738 , B01L7/52 , B01L2200/0668 , B01L2300/0838 , B01L2300/087 , B01L2400/0633 , G01N35/0098 , Y10T436/143333
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid purification apparatus and a nucleic acid purification method to use the nucleic acid purification apparatus. SOLUTION: The nucleic acid purification apparatus for a cell or virus is provided with a cytolytic capillary tube having a specimen introducing port and holding a specimen and magnetic beads introduced through the specimen introducing port, a vibrator attached to the cytolytic capillary tube and mixing the specimen and the magnetic beads in the cytolytic capillary tube, a laser generation part attached to the cytolytic capillary tube and supply laser light to the cytolytic capillary tube, and a magnetic force generation part attached to the cytolytic capillary tube to fix the magnetic beads to the wall of the cytolytic capillary tube. The invention further provides a nucleic acid purification method by using the nucleic acid purification apparatus. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供使用核酸纯化装置的核酸纯化装置和核酸纯化方法。解决方案：用于细胞或病毒的核酸纯化装置设置有细胞溶解毛细管，该细胞溶解毛细管具有试样导入口并保持试样和通过试样导入口引入的磁珠，附着在细胞溶解毛细管上的振动器，将试样和磁珠混合在细胞溶解毛细管中，激光产生部分附着于细胞溶解毛细管并向细胞溶解毛细管提供激光，以及磁力产生部分，附着于细胞溶解毛细管以固定磁珠到细胞溶解毛细管的壁。本发明还提供了使用核酸纯化装置的核酸纯化方法。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2006115841A Method and apparatus for destroying cell or virus 有权
标题翻译：破坏细胞或病毒的方法和装置
公开(公告)号：JP2006115841A
公开(公告)日：2006-05-11
申请号：JP2005304982
申请日：2005-10-19
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： LEE JEONG-GUN , KWON YOUNG-NAM , KIM YOUNG-A , LEE MYO-YONG , YOO SHIN-I , CHO YEON-JA , TEI KOKO , YOO CHANG-EUN , YANG SEUNG-YEON
IPC分类号： C12N15/09 , C12M1/00 , C12Q1/68
CPC分类号： C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
摘要： PROBLEM TO BE SOLVED: To provide a method for rapidly destroying cells or viruses with a laser and micro magnetic beads, and to provide an apparatus therefor. SOLUTION: This method for destructuring the cells or viruses comprises a step for injecting magnetic beads into a solution containing the cells or viruses, a step for vibrating the magnetic beads, and a step for irradiating laser beams on the magnetic beads to destroy the cells or viruses. And the apparatus for destroying the cells or viruses comprises a cell dissolution chamber in which a sample inlet is formed and through whose sample inlet a sample and magnetic beads are received, a vibrator which is mounted on the cell dissolution chamber and used for mixing the sample with the magnetic beads in the cell dissolution chamber, and a laser beam-generating portion which is mounted on the cell dissolution chamber and supplies laser beams. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供用激光和微磁珠快速破坏细胞或病毒的方法，并提供其装置。解决方案：用于破坏细胞或病毒的方法包括将磁珠注入含有细胞或病毒的溶液的步骤，用于使磁珠振动的步骤，以及用于在磁珠上照射激光束以破坏的步骤细胞或病毒。并且用于破坏细胞或病毒的装置包括细胞溶解室，其中形成样品入口，并且通过其接收样品和磁珠的样品入口，安装在细胞溶解室上并用于混合样品的振动器细胞溶解室中的磁珠和安装在细胞溶解室上并提供激光束的激光束产生部分。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2006149386A System and method for refining nucleic acid 有权
标题翻译：用于精制核酸的系统和方法
公开(公告)号：JP2006149386A
公开(公告)日：2006-06-15
申请号：JP2005339501
申请日：2005-11-24
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： LEE JEONG-GUN , KWON YOUNG-NAM , LEE MYO-YONG , YOO SHIN-I , CHO YEON-JA , KIM YOUNG-A
IPC分类号： C12N15/09 , C12M1/00
CPC分类号： B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2300/0681 , B01L2300/0867 , B01L2300/087 , B01L2400/0487
摘要： PROBLEM TO BE SOLVED: To provide a system for refining a nucleic acid using beads differing in laser absorption degree from each other, and to provide a method for refining the nucleic acid using the system.
SOLUTION: The system for refining a nucleic acid in cells or virus comprises a cytolytic capillary provided with a sample inlet and functioning to accommodate via the inlet a sample, magnetic beads and a solid substrate therein, a vibrator mounted on the capillary and functioning to mix the sample, the magnetic beads and the solid substrate together, a laser generator mounted on the capillary and functioning to supply laser to the capillary, a magnetic force generator mounted on the capillary and functioning to fix the magnetic beads onto the wall of the capillary, a discharge chamber mounted on the capillary and functioning to discharge a lysate, an elution buffer chamber mounted on the capillary and functioning to elute the nucleic acid from the solid substrate bound with the nucleic acid, and a neutralization buffer chamber mounted on the capillary and functioning to neutralize a nucleic acid solution eluted. The method for refining the nucleic acid using the system is also provided.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供使用激光吸收度不同的珠的精制精制系统，并提供使用该系统精制该核酸的方法。解决方案：用于在细胞或病毒中精制核酸的系统包括提供有样品入口的细胞溶解毛细管，并且用于经由入口容纳样品，磁珠和其中的固体基质，安装在毛细管上的振动器，功能是将样品，磁珠和固体基底混合在一起，安装在毛细管上并用于向毛细管提供激光的激光发生器，安装在毛细管上的磁力发生器，并且用于将磁珠固定在毛细管，安装在毛细管上并用于排出裂解物并用于排出裂解物的排放室，安装在毛细管上并用于从核酸结合的固体底物洗脱核酸的洗脱缓冲室，以及安装在毛细管上的中和缓冲室毛细管并且功能来中和洗脱的核酸溶液。还提供了使用该系统来精制该核酸的方法。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2006068011A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2006068011A
公开(公告)日：2006-03-16
申请号：JP2005246323
申请日：2005-08-26
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: This method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme, and then calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding background fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：用于从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或扩增对应于不包括背景荧光信号的最大荧光信号的强度的一半，对应于最大扩增效率的放大循环次数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2009106298A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2009106298A
公开(公告)日：2009-05-21
申请号：JP2008313681
申请日：2008-12-09
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data comprises: a step of amplifying a nucleic acid (DNA or RNA) extracted from an organism or virus with an enzyme; a step of generating a function representing correlation with strength of fluorescent signal by amplification amount shown in real time by amplification cycle number or amplification time of the nucleic acid; a step of calculating a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals of the nucleic acid by utilizing the function; and a step of obtaining initial concentration of the nucleic acid from the calculated unamplified fluorescent light signal strength. Thereby, the early concentration of the nucleic acid can be determined without using a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括：用酶扩增从生物体或病毒提取的核酸（DNA或RNA）的步骤; 通过扩增循环次数或核酸扩增时间实时显示荧光信号强度的相关性函数的步骤; 通过利用该功能计算除了核酸的背景荧光信号之外的未扩增核酸荧光信号的强度的步骤; 以及从计算的未扩增的荧光信号强度获得核酸的初始浓度的步骤。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2009072206A Method for determining early nucleic acid concentration from real time nucleic acid amplification data 有权
标题翻译：从实时核酸扩增数据确定早期核酸浓度的方法
公开(公告)号：JP2009072206A
公开(公告)日：2009-04-09
申请号：JP2008313682
申请日：2008-12-09
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： NAMKOONG KAK , KIM JIN-TAE , LEE YOUNG-SUN , KIM YOUNG-A
IPC分类号： C12Q1/68 , G01N21/78
CPC分类号： C12Q1/6851
摘要： PROBLEM TO BE SOLVED: To provide a method for determining an early nucleic acid concentration from real time nucleic acid amplification data.
SOLUTION: The method for determining the early nucleic acid concentration from the real time nucleic acid amplification data includes amplifying a nucleic acids (DNA or RNA) extracted from an organism or virus with an enzyme, and then, calculating amplification cycle number or amplification time corresponding to one half of the strength of the maximum fluorescent light signal excluding back ground fluorescent signals, amplification cycle number or amplification time corresponding to the maximum amplification efficiency, and a strength of unamplified nucleic acid fluorescent light signal excluding the background fluorescent signals. Thereby, the early concentration of the nucleic acid can be determined without a differentiation/integration method.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供从实时核酸扩增数据确定早期核酸浓度的方法。解决方案：从实时核酸扩增数据确定早期核酸浓度的方法包括用酶扩增从生物或病毒提取的核酸（DNA或RNA），然后计算扩增循环数或对应于不包括背景荧光信号的最大荧光信号的强度的一半的放大时间，对应于最大扩增效率的扩增循环数或扩增时间，以及不包括背景荧光信号的未扩增核酸荧光信号的强度。因此，可以不使用分化/积分法来确定核酸的早期浓度。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2007139762A Biosensor, manufacturing method therefor, and method of detecting biomolecule using biosensor 审中-公开
标题翻译：生物传感器，其制造方法和使用生物传感器检测生物分子的方法
公开(公告)号：JP2007139762A
公开(公告)日：2007-06-07
申请号：JP2006287636
申请日：2006-10-23
申请人： Samsung Electronics Co Ltd , 三星電子株式会社Samsung Electronics Co.,Ltd.
发明人： SHIM JEO-YOUNG , LEE KYU-SANG , YOO CHANG-EUN , HWANG KYU-YOUN , KIM YOUNG-A , YOO KYU-TAE , CHO YEON-JA
IPC分类号： G01N27/414 , C12M1/00 , C12M1/34 , C12Q1/68 , G01N37/00
CPC分类号： G01N33/54373 , B01J2219/00529 , B01J2219/00596 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00659 , B01J2219/00722 , G01N27/4145
摘要： PROBLEM TO BE SOLVED: To provide a biosensor manufactured without executing an additional process other than a semiconductor manufacturing process, and capable of detecting efficiently a micro amount of biomolecule.
SOLUTION: This biosensor includes a field-effect transistor having a substrate, a source and a drain having a polarity opposite to that of the substrate, a gate arranged on the substrate and contacting with the source and the drain, and an inorganic membrane coupled with the biomolecule and arranged on a surface of the gate. The present invention discloses also a manufacturing method for the biosensor including the field-effect transistor, and a method of detecting the biomolecule using the biosensor including the field-effect transistor.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种不需要执行半导体制造工艺以外的附加处理而制造的生物传感器，并能够有效地检测微量的生物分子。解决方案：该生物传感器包括场效应晶体管，其具有基板，源极和漏极，其极性与基板的极性相反，栅极布置在基板上并与源极和漏极接触，膜与生物分子结合并且布置在栅极的表面上。本发明还公开了包括场效应晶体管的生物传感器的制造方法以及使用包括场效应晶体管的生物传感器检测生物分子的方法。版权所有（C）2007，JPO＆INPIT

8. 发明专利

JP2005058234A Pcr primer set for detecting hepatitis b, and method for detecting hepatitis b and using the primer set 审中-公开
标题翻译：用于检测乙型肝炎的PCR引物组和用于检测乙型肝炎和使用PRIMER SET的方法
公开(公告)号：JP2005058234A
公开(公告)日：2005-03-10
申请号：JP2004231611
申请日：2004-08-06
申请人： Samsung Electronics Co Ltd , 三星電子株式会社
发明人： LEE YOUNG-SUN , HAN JUNG-IM , HWANG JUNG-JOO , KIM MI-KYUNG , CHO YOON-KYOUNG , LIM HEE-KYUN , KIM YOUNG-A
IPC分类号： C12N15/09 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/706 , C12Q2565/629 , C12Q2565/543 , C12Q2531/113
摘要： PROBLEM TO BE SOLVED: To provide a PCR primer set for detecting hepatitis B, and to provide a method for detecting hepatitis B and using the primer set. SOLUTION: Provided are a primer set selected from a group consisting of primers having a specific base sequence, a method for detecting hepatitis B and including a step of PCR by using the primer set, and a hepatitis B detection kit including the primer set. Thereby, hepatitis B is quickly and accurately diagnosed. COPYRIGHT: (C)2005,JPO&NCIPI

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