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1. 发明专利

JPH04211360A NEW LACTIC ACID BACTERIUM, ANTIMICROBIAL SUBSTANCE PRODUCED THEREBY, STARTER FOR FERMENTING MILK CONTAINING THE SAME BACTERIUM AND PRODUCTION OF FERMENTED MILK USING THE SAME STARTER 失效
公开(公告)号：JPH04211360A
公开(公告)日：1992-08-03
申请号：JP4144391
申请日：1991-01-26
申请人： SNOW BRAND MILK PROD CO LTD
发明人： SASAKI MASAHIRO , ISHII SATORU , YAMAUCHI YOSHIHIKO , KITAMURA KATSUSHI , TOYODA SHUJI , AHIKO KENKICHI
IPC分类号： A01N63/00 , A01N63/02 , A23C9/12 , A23C9/123 , C07K4/04 , C07K14/005 , C07K14/195 , C07K14/315 , C07K14/37 , C07K14/41 , C12N1/20 , C12P21/00 , C12R1/46
摘要： PURPOSE:To obtain new lactic acid bacteria producing new antimicrobial substance capable of reducing raise of acidity of a product and belonging to Spreptococcus salivarius subsp. thermophilus. CONSTITUTION:Streptococcus salivarius subsp. thermophilus SBT 1277 (FERM No. 11155, FERM BP-3234) is cultured in SVL culture medium and the cultured mixture is fractionated using 30-70% ammonium sulfate to provide the antimicrobial substance having the following properties as a precipitate: (A) being a peptide, protein or complex containing the protein, (B) being digested by trypsin, (C) being not digested by phospholipase D and (D) exhibiting thermal stability to heating at pH 8.0 and 160 deg.C for 30min. The above-mentioned thermophilus exists in raw cow milk and separated therefrom and immobilized.

2. 发明专利

JPH01171487A RECOMBINANT PLASMID PSI878 失效
公开(公告)号：JPH01171487A
公开(公告)日：1989-07-06
申请号：JP32963987
申请日：1987-12-28
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , HASHIBA EN
IPC分类号： C12N15/09 , C12N15/00 , C12N15/74 , C12R1/125 , C12R1/19 , C12R1/46
摘要： PURPOSE:To construct the title plasmid having broad host range and useful in the field of fermentation industry, by including a gene originated from E.coli, etc., and a gene originated from Streptococcus faecalis DS5, etc., in a plasmid. CONSTITUTION:An ampicillin resistant gene originated from plasmid pBR329 of E.coli, a chloramphenicol resistant gene and a replicating region are linked with an erythromycin resistant gene originated from plasmid pAMbeta1 of Streptococcus faecalis DS5 using T4DNA ligase. Escherichia coli V850 is transformed with the resultant linked DNA, an E.coli having plasmid pS1878 is selected from the obtained transformat and the cell is cultured in a medium to obtain the cell of E.coli V850. The cell is extracted to obtain a recombinant plasmid pS1878 containing about 1,080 base pairs, having only one incision site with restriction enzymes Ball, PstI and NcoI and able to be proliferated in E.coli, Bacillus subtilis and lactobacillus.

3. 发明专利

JPH05124920A METHOD FOR REMOVING BACTERIOPHAGE AND WASHING AGENT AND FILTERING AGENT TO BE USED THEREFOR 失效
公开(公告)号：JPH05124920A
公开(公告)日：1993-05-21
申请号：JP28672191
申请日：1991-10-31
申请人： SNOW BRAND MILK PROD CO LTD
发明人： AKAI YOSHIHITO , SASAKI MASAHIRO , SUZUKI YUTAKA , ISHII SATORU
IPC分类号： A01N25/34 , A01N63/00 , C11D7/46
摘要： PURPOSE:To highly and effectively prevent or remove the contamination of lactic fermentation product with bacteriophage over a wide pH range by washing a production apparatus for lactic fermentation product with a chitin or chitosan solution. CONSTITUTION:The washing agent to be used in the subject treatment is produced by dissolving chitin or chitosan in 1% aqueous solution of acetic acid, mixing 1 pt. of the solution to 100 pts. of a 0.1M phosphoric acid buffering solution and adding 0.1 pts. of xanthan gum as a thickening agent to the mixture. The agent is sprayed to a production apparatus for lactic fermentation product such as yogurt, butter and cheese, the apparatus is washed after 3hr and the washing liquid of the apparatus is filtered with a porous material composed of chitin or chitosan and a fibrous material (e.g. pulp) to effectively and highly remove or prevent the contamination with bacteriophage.

4. 发明专利

JPH04278079A PHAGE-RESISTANT LACTIC ACID STRAIN AND FERMENTED FOOD USING THE SAME 失效
公开(公告)号：JPH04278079A
公开(公告)日：1992-10-02
申请号：JP7744291
申请日：1991-03-01
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , KASAGAWA TOMOKO , TOYODA SHUJI , AHIKO KENKICHI
IPC分类号： A23C9/12 , A23C13/16 , A23C15/02 , A23C19/032 , C12N1/20 , C12R1/46
摘要： PURPOSE:To provide the title strain and fermented food obtained using this strain. CONSTITUTION:The objective phage-resistant Streptococcus lactis subsp. diacetylactis and fermented foods such as fermented butter, lactic acid beverages and cheese obtained by fermentation using this strain. Use of the present strain will prevent phage contamination in producing these foods and facilitate the production process control.

5. 发明专利

JPH04126093A PRODUCTION OF NISIN AND NISIN-PRODUCING MICROORGANISM 失效
公开(公告)号：JPH04126093A
公开(公告)日：1992-04-27
申请号：JP24678790
申请日：1990-09-17
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , HASHIBA EN
IPC分类号： C12N1/20 , C12P21/02 , C12R1/46
摘要： PURPOSE:To stably enhance production efficiency of nisin by culturing a nisin producing microorganism in a culture medium to which a specific amino acid is added. CONSTITUTION:Nisin producing lactic acid bacterium (A) of SBT 1212 (FERM P 11674) or SBT 1227 (FERM P-11675), etc., belonging to Streptococcus lactis and retaining a nisin producing gene in chromosome is collected by selecting nisin by nisin-sensitive test and nisin producing test, etc. Then one or more kind of amino acid (e.g. serine, phenylalanine or glycine) such as (a) amino acids necessary to rearing of the bacterium 4, (b) amino acids contained in primary structure of nisin, (c) amino acids becoming a precursor of these amino acids in metabolism path is added thereto at an amount of 0.1-10mg/ml to provide a culture medium (B). A cultured mixture obtained by inoculating the culture medium B with the bacterium A and then culturing the bacterium by shake culture is subjected to extraction purification by chloroform extraction method, etc., to produce the nisin.

6. 发明专利

JPH09238614A PRODUCTION OF WHEY 失效
公开(公告)号：JPH09238614A
公开(公告)日：1997-09-16
申请号：JP7817996
申请日：1996-03-06
申请人： SNOW BRAND MILK PROD CO LTD
发明人： TANIGUCHI KIYOTAKA , SERIZAWA ATSUSHI , FUJII TOMOYUKI , ISHII SATORU , HORIKAWA MASAKAZU
IPC分类号： A23C9/15 , A23C9/18 , A23C21/06 , A23J3/08
摘要： PROBLEM TO BE SOLVED: To obtain white whey capable of being widely used as a food raw material, excellent in thermal stability, exhibiting high solubility and free from the smell of whey. SOLUTION: Whey obtained as a by-product on the production of cheese or casein is controlled to a pH of 5.5-6.5, and subsequently heated at a temperature of >=90 deg.C to convert whey proteins contained in the whey into micelles. The time for the heating depends on the concentrations of the proteins contained in the whey of raw material, but is e.g. 2-5sec at 120 deg.C, When the whey is used for the production of cheese. The obtained white whey is subsequently mixed with casein in an amount of >=1/20wt.% based on the whey proteins. The obtained stable suspension is heated at 120 deg.C and subsequently spray-dried to obtain the whey powder.

7. 发明专利

JPH06113899A METHOD AND KID FOR SIMPLY IDENTIFYING BUTYRIC ACID BACTERIUM 失效
公开(公告)号：JPH06113899A
公开(公告)日：1994-04-26
申请号：JP28552592
申请日：1992-09-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , SAKAGUCHI SETSU , KASAGAWA TOMOKO , SUZUKI YUTAKA
IPC分类号： C12N15/09 , C12N15/10 , C12Q1/04 , C12Q1/68 , C12R1/145 , G01N33/50
摘要： PURPOSE:To simply and accurately identify butyric acid bacterium without requiring a complicated device or a complex technique by extracting DNA from the butyric acid bacterium, multiplying a rRNA gene by a PCR method using a specific primer, treating the multiplied rRNA gene with a restriction enzyme, analyzing the treated product by an electrophoresis method, and subsequently comparing the analysis result. CONSTITUTION:Chromosomal DNA is extracted from a butyric acid bacterium such as Clostoridium tyrobutyricum, Clostoridium butyricum, Clostoridium sporogenes or their analogous bacterium. Subsequently, the rRNA gene (rDNA) is multiplied by a PCR method using a specific primer, and cleaved with a restriction enzyme such as Hhal, Rsal or Sau3AI. The length of the obtained rDNA fragment is analyzed and compared by an electrophoresis method to simply and accurately identify the butyric acid bacterium.

8. 发明专利

JPH02167070A PREPARATION OF LACTOBACILLUS BIFIDUS PROTOPLAST 失效
公开(公告)号：JPH02167070A
公开(公告)日：1990-06-27
申请号：JP20158088
申请日：1988-08-12
申请人： SNOW BRAND MILK PROD CO LTD
发明人： AOYAMA KENJI , ISHII SATORU , HASHIBA EN
IPC分类号： C12N1/20 , C12N1/21 , C12N15/02 , C12R1/01
摘要： PURPOSE:To obtain the title favorable spherical protoplast useful for protoplast fusion by treating a bacterial suspension of Bifidobacterium longum in the presence of, as cell-wall digesting enzyme, a combination of lysozyme and actinase E. CONSTITUTION:A bacterial suspension of Bifidobacterium longum [e.g. Bifidobacterium longum SBT 2933-R (FERM-8743)] is treated either in the presence of, as cell-wall digesting enzyme, a combination of lysozyme and actinase E or proteinase K, or treated with lysozyme followed by actinase E or proteinase K, thus obtaining the objective protoplast. The latter treatment process is preferable because of being better in respect of protoplast fusion.

9. 发明专利

JPH0276572A MEDIUM FOR REGENERATING BIFFIDOBACTERIUM PROTOPLAST AND REGENERATION THEREOF USING SAME MEDIUM 失效
公开(公告)号：JPH0276572A
公开(公告)日：1990-03-15
申请号：JP22834288
申请日：1988-09-14
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , AOYAMA KENJI , HASHIBA EN
IPC分类号： C12N1/20 , C12R1/01
摘要： PURPOSE:To regenerate the protoplast of biffidobacterium in a high yield by smearing the biffidobacterium protoplast on a specific composition medium mixed an osmotic stabilizer and subsequently culturing the protoplast under an anaerobic condition. CONSTITUTION:MNM-G medium having a composition 2.5g of K2HPO4, 10g of CH3COONa, 10g of tryptone, 5.0g of a yeast extract, 0.4g of cysteine.HCl.H2 O, 5.0g of glucose, 15g of a bact agar, 5mM of CaCl2.2H2O, 5mM of MgCl2.6H2 O and a pH of 6.5 is mixed with an osmotic stabilizer raffinose in a concentra tion of 0.2-0.3M. A biffidobacterium.pseudolongum or biffidobacterium.longum protoplast suspension diluted with a hypertonic buffer solution is smeared on the prepared medium and cultured under an anaerobic condition.

10. 发明专利

JPH08256682A PRODUCTION OF LOW LACTOSE POWDERED MILK 失效
公开(公告)号：JPH08256682A
公开(公告)日：1996-10-08
申请号：JP6624195
申请日：1995-03-24
申请人： SNOW BRAND MILK PROD CO LTD
发明人： ISHII SATORU , SUZUKI YUTAKA
IPC分类号： A23C9/18 , C12N1/16 , C12R1/865
摘要： PURPOSE: To produce a low lactose powdered milk product useful for alimenting a lactose-intolerant patient since it contains no lactose and free from the browning attributable to lactose by carrying out alcohol fermentation on the lactose in a raw-material milk followed by spray-drying. CONSTITUTION: After carrying out alcohol fermentation on the lactose in a raw-material milk (e.g. skim milk), the alcohol is made to evaporate by spray drying and the objective product is obtained. Simultaneous addition of yeast and a lactose-decomposing enzyme to the raw-material milk or the addition of yeast to the milk treated with the lactose-decomposing enzyme allows alcohol fermentation with the yeast on the glucose and galactose which are formed by the decomposition of lactic acid. This process, preferably carrying out alcohol fermentation on lactic acid, prevents the generation of foul smell and attains excellent fermentation efficiency. As the yeast, Saccharomyces cereviciae SBT5211 strain (FERM-P-14819) is preferable. Further, as the lactose- decomposing enzyme, the one free from protein decomposing activity is preferred.

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