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1. 发明专利

JPH1123534A METHOD AND DEVICE FOR MEASURING HYPOCHLOROUS ACID 失效
公开(公告)号：JPH1123534A
公开(公告)日：1999-01-29
申请号：JP19046197
申请日：1997-06-30
申请人： SHIMADZU CORP
发明人： JIKUYA HIROYUKI , ETANI HAJIME
IPC分类号： G01N27/416 , G01N27/48
摘要： PROBLEM TO BE SOLVED: To quantitatively determine hypochlorous acid concentration by a simple device. SOLUTION: A sample water 8 is put in a vessel 10, and a working electrode 2, a counter pole 4, and a reference electrode 6 are immersed into the sample water 8. A constant voltage between -0.2 V and -0.6 V is applied to the working electrode 2 by a measuring part 12 making a reference of the potential of the reference electrode 6, and the current flowing between the counter pole 4 and the working electrode 2 is detected. An arithmetic unit 14 obtains and outputs the concentration of hypochlorite by applying calibration-curve data to the current detected by the measuring part 12.

2. 发明专利

JPH10108669A MICROORGANISM CAPABLE OF DECOMPOSING POLYLACTATE RESIN AND MICROBIAL DECOMPOSITION OF POLYLACTATE RESIN 失效
公开(公告)号：JPH10108669A
公开(公告)日：1998-04-28
申请号：JP26207396
申请日：1996-10-02
申请人： AGENCY IND SCIENCE TECHN , SHIMADZU CORP
发明人： TOKIWA YUTAKA , JIKUYA HIROYUKI , NAGAI NAOKO
IPC分类号： C12N1/20 , B09B3/00 , C08J11/06 , C12P1/06 , C12R1/465 , C12S99/00 , C12S13/00
摘要： PROBLEM TO BE SOLVED: To obtain a microorganism belonging to the genus Streptomyces (FERM P-1.5869), capable of decomposing polylactate resin without exhausting gases in contrast to the incineration treatment, saving the time compared with land disposal and having extremely high value from the viewpoint of waste processing. SOLUTION: A microorganism belonging to the genus Streptomyces and capable of decomposing polylactate resin (FERM P-15869), preferably Streptomyces violaceusniger or Streptomyces cyaneus is separated from the soil, river water or lake water collected in the Tsukuba City, Ibaraki Prefecture, Japan.

3. 发明专利

JPH05103658A CAPSULE SCREENING SYSTEM 失效
公开(公告)号：JPH05103658A
公开(公告)日：1993-04-27
申请号：JP28050591
申请日：1991-09-30
申请人： SHIMADZU CORP
发明人： ABE HIROHISA , TAKANO JUN , JIKUYA HIROYUKI , AOYAMA YOSHIHIRO , OKADA MANAMI
IPC分类号： C12M1/34 , C12N5/10 , G01N33/577
摘要： PURPOSE:To perform the title screening automatically by measuring the fluores cence intensity, etc., of planarly arranged capsules and by fitting the tip of a freely movable manipulator with a pipette followed by moving it to the aimed capsule. CONSTITUTION:The absorbance or fluorescence intensity of each of planarly arranged capsules is determined to search such capsules with the characteristic value at or higher than a specified level. The tip of a manipulator movable in the x, y and z directions is then fitted with a pipette, and its tip is made to move to the aimed capsule position. A water or air jet from the pipette is allowed to impinge on the capsules to separate them from the other ones through the meshes in a capsule-holding vessel; alternately, the aimed capsules are put to suction with the pipette and separated from the others. Thereby, only the capsules with fluorescence intensity at or higher than a specified value can be collected.

4. 发明专利

JPH0576392A CAPSULE SCREENING SYSTEM 失效
公开(公告)号：JPH0576392A
公开(公告)日：1993-03-30
申请号：JP27017691
申请日：1991-09-21
申请人： SHIMADZU CORP
发明人： ABE HIROHISA , TAKANO JUN , JIKUYA HIROYUKI , AOYAMA YOSHIHIRO , OKADA MANAMI
IPC分类号： C12M1/34 , C12N5/10 , C12Q1/00
摘要： PURPOSE:To provide the title system of specific constitution, capable of quantitatively detecting whether aimed cells such as monoclonal antibody-productive cells are contained in capsules or not and also of making the screening operation accurately in high speed. CONSTITUTION:Firstly, the color intensity of capsules dyed with a dyeing agent is detected by a detecting means followed by sending the capsules to a droplet- forming means to form droplets each containing a single capsule. Thence, according to the detection result mentioned above, voltage is applied on only droplets containing such capsules with color intensity either out of the range of a specified value or within such range to electrically charge them, and such droplets charged are electrically put out of the flow channel to perform the objective screening. The dyeing is carried out in such a way, for example, that a substance (e.g. protein A) labeled with a dyeing agent is conjugated with the aimed substance in the capsules.

5. 发明专利

JPH114680A BACTERIA DECOMPOSING POLYLACTIC ACID RESIN AND MICROBIAL DEGRADATION OF POLYLACTIC ACID RESIN 失效
公开(公告)号：JPH114680A
公开(公告)日：1999-01-12
申请号：JP16023097
申请日：1997-06-17
申请人： AGENCY IND SCIENCE TECHN , SHIMADZU CORP
发明人： TOKIWA YUTAKA , NAGAI NAOKO , JIKUYA HIROYUKI
IPC分类号： C12N1/20 , B09B3/00 , C08J11/04 , C08L101/16 , C12R1/07 , C12R1/09 , C12R1/125 , C12S99/00
摘要： PROBLEM TO BE SOLVED: To provide a converting method of polylactic acid resin into useful materials such as organic acids, etc., or composts without producing exhaust gas such as incineration by decomposing the polylactic acid resin with fungus belonging to genus Bacillus. SOLUTION: The polylactic acid resins such as poly-L-lactic acid, etc., are decomposed by the fungus belonging to genus Bacillus such as Bacillus subtilis (FERM P-16181), Bacillus circulans (FERM P-16182), Bacillus stearothermophilus (FERM P-16183), etc., free from pollution of the environment.

6. 发明专利

JPH08271479A SAMPLE INJECTOR FOR ELECTROPHORESIS 失效
公开(公告)号：JPH08271479A
公开(公告)日：1996-10-18
申请号：JP7146095
申请日：1995-03-29
申请人： SHIMADZU CORP
发明人： JIKUYA HIROYUKI
IPC分类号： G01N27/447
摘要： PURPOSE: To provide an injector for injecting a plurality of sampled simultaneously while preventing lateral leakage by sucking a sample into a comb part through capillarity and fitting the comb part to the sample well in an electrophoretic unit thereby causing electrophoresis. CONSTITUTION: A comb part 3 is provided with glass combs at a constant interval where the number and interval of combs correspond to the number and interval of sample wells. The space defined by the comb part 1, a spacer 3 and a front plate 2 serves as a capillary S for sucking a sample. The comb part 1 is inserted into a sample container in order to suck the sample into the capillary S and then the comb part 1 is fitted to the sample well in an electrophoretic unit thus causing electrophoresis. An electrophoretic gel 7 is immersed, at the opposite ends, into electrode tanks 5, 6 containing an electrolyte. An electrophoretic voltage is then applied from an electrophoretic power supply 8 between the electrode tanks 5, 6. The electrophoretic get 7 is provided, at one end with a sample well 10 for injecting the sample and a sample is injected to each specified position for each end base.

7. 发明专利

JPH03147797A DETECTION OF NUCLEIC ACID 失效
公开(公告)号：JPH03147797A
公开(公告)日：1991-06-24
申请号：JP28432289
申请日：1989-10-31
申请人： SHIMADZU CORP
发明人： TADA ATSUSHI , YAMAGATA KOICHI , JIKUYA HIROYUKI
IPC分类号： C12Q1/68 , C12N15/09
摘要： PURPOSE:To detect nucleic acid in a simple manner and accomplish automation of its detection by hybridizing DNA and an oligonucleotide having sequence complementary to part of said DNA in a solution followed by separating the solution taking advantage of molecular weight difference. CONSTITUTION:Firstly, in a solution, the aimed DNA or RNA is hybridized with an oligonucleotide having base sequence complementary to part thereof. Thence, this solution is treated using a method taking advantage of molecular weight or electric charge difference to separate and remove the oligonucleotide probes having failed to form complementary strand, thus detecting the base sequence of a specific nucleic acid. Specifically, it is suggested that using a radiolabeled oligonucleotide, the aimed nucleic acid can be detected with e.g. an RI detector.

8. 发明专利

JPH0349696A OLIGONUCLEOTIDE FOR DETECTING BACTERIA AND METHOD FOR DETECTION USING THE SAME 失效
公开(公告)号：JPH0349696A
公开(公告)日：1991-03-04
申请号：JP18568189
申请日：1989-07-18
申请人： SHIMADZU CORP
发明人： OHASHI TETSUO , JIKUYA HIROYUKI , TAKANO JUN
IPC分类号： C12Q1/04 , C12Q1/68 , C12R1/085
摘要： PURPOSE:To enable simple and rapid detection with a high sensitivity by carrying out polymerase chain reaction(PCR) treatment using a specific oligonucleotide functioning as a primer for nucleic acid synthetic reaction. CONSTITUTION:Microbial cells of Bacillus cereus, such as Bacillus cereus strain, are extracted to provide a specimen (A) composed of a nucleic acid ingredient. The same sequence as the base sequence of beta-lactamase I type and II type is chemically synthesized to afford a synthetic nucleotide (B) composed of a sequence group expressed by the formula. The ingredients (A) and (B) and a thermostable DNA polymerase are dissolved in a buffer solution to afford a reaction solution (C). The obtained ingredient (C) is then thermally denatured at about 94 deg.C, subsequently annealed and further subjected to polymerization reaction. The PCR is performed in about 42 cycles to afford an amplified nucleotide fragment (D). The resultant ingredient (D) is then subjected to agarose electrophoresis, etc., to calculate the length of the nucleotide fragment and selectively detect the Bacillus cereus in the specimen.

9. 发明专利

JPH02131598A DNA PROBE TO BE USED IN SALMONELLA ASSESSMENT AND ASSESSMENT OF SALMONELLA USING THE SAME 失效
公开(公告)号：JPH02131598A
公开(公告)日：1990-05-21
申请号：JP15605489
申请日：1989-06-19
申请人： SHIMADZU CORP
发明人： JIKUYA HIROYUKI , OHASHI TETSUO , TAKANO JUN
IPC分类号： C12Q1/10 , C12Q1/68
摘要： PURPOSE:To enable simple and inexpensive detection of Salmonella in a shortened time by bringing a specific probe into contact with a sample under such condition as the prove will hybridize with Salmonella DNA. CONSTITUTION:The DNA of Salmonella is fragmented with a restriction enzyme or the like into the DNA fragment or RNA fragment (A) which can hybridize with the base sequence of formula I or II, in which the latter is complementary to formula I. Then, a labeled substance such as a radioactive isotope is directly bonded to the fragment A to obtain DNA probe (B). Then, the bacterium cells are broken with a bacteriolytic enzyme to collect DNA and the DNA is immobilized on a membrane such as nylon, and an excessive amount of DNA prove (B) is added to form hybrid (C). The non-hybrid probes in (C) are removed by washing and the labeled substance in (C) is measured with a scintillation counter to detect Salmonella.

10. 发明专利

JPH11344439A ABSORPTIOMETRIC ANALYTICAL INSTRUMENT 失效
公开(公告)号：JPH11344439A
公开(公告)日：1999-12-14
申请号：JP15255698
申请日：1998-06-02
申请人： SHIMADZU CORP
发明人： KACHI TORU , NAKAMURA KENJI , JIKUYA HIROYUKI , ISHIKAWA KUNIO , IWAI NOBUYUKI , TOMINAGA HIDEKI
IPC分类号： G01N21/27
摘要： PROBLEM TO BE SOLVED: To conduct measurement without pretreatment such as the dilution of a sample and remeasurement even in the case where a detection limit is exceeded in a measurement wavelength for obtaining a high resolution. SOLUTION: This analytical instrument is provided with a light source 1 for measuring absorbance according to a plurality of measurement wavelengths and a photometry part 3 including a light detection means 4. Then, the photometry part 3 is provided with a plurality of calibration curves for obtaining a sample concentration from the absorbance by making different the measurement wavelengths and concentration ranges, and at the same time selects a measurement wavelength from a plurality of measurement wavelengths, and selects a calibration curve corresponding to the selected measurement wavelength from a plurality of calibration curves, and obtains a sample concentration corresponding to the absorbence according to the selected measurement wavelength based on the measurement calibration curve. By measuring absorbence at a plurality of wavelengths and detecting concentration by using calibration curves with different measurement wavelengths, concentration is measured always in the inside of the detection boundary of the measurement part, thus the pretreatment and remeasurement of the dilution or the like of a sample which are generated the concentration of the measurement sample exceeding the measurement boundary at a high resolution wavelength can be avoided.

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