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    • 4. 发明专利
    • Method for producing meat-processed product
    • 生产肉类加工产品的方法
    • JP2010142169A
    • 2010-07-01
    • JP2008323523
    • 2008-12-19
    • Prima Meat Packers Ltdプリマハム株式会社
    • TAKANO TAKESHINAKAGAWA TOSHIYUKINAMIOKA MAKOTONAKAE SATOE
    • A22C13/00
    • PROBLEM TO BE SOLVED: To provide a method for producing processed meat products such as a sausage by using collagen casings for the casing of the processed meat products in substitution for natural intestine, having texture such as crispness, resilience, crunchy feeling, hardness, or the like similar to those of the natural intestine, having reduced collagen smell, also having gloss on appearance when the collagen casing is cased into the processed meat products, while the casing has a good sliding property, and capable of improving workability, in the working of filling time.
      SOLUTION: The processed meat raw material casings are obtained by immersing the collagen casings having been subjected to drying treatment in an alkaline solution selected from solutions of phosphate, carbonate, and sodium hydroxide, and using the collagen casings after the immersion treatment for casing the processed meat raw material. The phosphate is preferably selected from sodium salts, potassium salts, calcium salts, and ammonium salts, and especially is trisodium phosphate.
      COPYRIGHT: (C)2010,JPO&INPIT
    • 要解决的问题:提供一种通过使用加工肉制品的肠衣的胶原蛋白肠替代天然肠道来制造加工肉制品如香肠的方法,具有脆性,弹性,脆性感等质地, 硬度等与天然肠的类似,具有降低的胶原蛋白气味,当胶原壳体装入加工肉制品时,外观上也具有光泽,同时壳体具有良好的滑动性能,并且能够提高加工性, 在填补时间的工作。 解决方案:加工的肉原料肠衣是通过将经过干燥处理的胶原蛋白壳浸渍在选自磷酸盐,碳酸盐和氢氧化钠的溶液中的碱性溶液中,并且在浸渍处理后使用胶原蛋白壳体获得的 包装加工肉类原料。 磷酸盐优选选自钠盐,钾盐,钙盐和铵盐,特别是磷酸三钠。 版权所有(C)2010,JPO&INPIT
    • 5. 发明专利
    • Detecting method for buckwheat allergen
    • BUCKWHEAT过敏检测方法
    • JP2009271092A
    • 2009-11-19
    • JP2009191202
    • 2009-08-20
    • Prima Meat Packers Ltdプリマハム株式会社
    • AKIMOTO MASANOBUKATO SHIGESHIRONAMIOKA MAKOTO
    • G01N33/53C07K16/16C07K16/18C12N5/10C12N15/02G01N33/68
    • C07K16/16C07K16/18G01N33/5308G01N33/68G01N2333/4731Y10S530/85Y10S530/868
    • PROBLEM TO BE SOLVED: To provide a highly sensitive immunological detection method whereby an allergen (for example, milk allergen, egg albumen allergen, wheat allergen, buckwheat allergen or peanut allergen) contained in a food can be detected in any state (i.e., either denatured or undenatured), and a detection kit to be used therein. SOLUTION: The detection method of an allergen uses two or more monoclonal antibodies capable of recognizing milk allergen in the undenatured and denatured states, egg albumen allergen in the undenatured and denatured states, wheat allergen in the undenatured and denatured states, buckwheat allergen in the undenatured and denatured states, or peanut allergen in the undenatured and denatured states, wherein use is made of, as an indication, αs1αs1 casein which is the major protein in αs1 casein, β-lactoglobulin which is the major protein in whey, ovalbumin and ovomucoid which are the major proteins in egg albumen, gliadin which is the major protein in wheat, proteins of 24 kDa and 76 kDa in molecular weight which are the major proteins in buckwheat, or Ara h1 which is the major protein in peanut. COPYRIGHT: (C)2010,JPO&INPIT
    • 要解决的问题:为了提供高度敏感的免疫检测方法,其可以在任何状态下检测食物中包含的变应原(例如,牛奶变应原,蛋清变应原,小麦变应原,荞麦变应原或花生过敏原) 即变性或未变性)和其中使用的检测试剂盒。 解决方案:变应原的检测方法使用两种或更多种能够识别未变性和变性状态的牛奶过敏原的单克隆抗体,未变性和变性状态的蛋清过敏原,未变性和变性状态的小麦变应原,荞麦变应原 在未变性和变性状态下,或未变性和变性状态的花生过敏原,其中使用作为αs1酪蛋白中主要蛋白质的αs1αs1酪蛋白,作为乳清中主要蛋白质的β-乳球蛋白,卵清蛋白 卵蛋白中的主要蛋白质,卵母细胞蛋白质,小麦中主要蛋白质的麦醇溶蛋白,作为荞麦主要蛋白质的分子量为24kDa和76kDa的蛋白质,或作为花生中主要蛋白质的Ara h1。 版权所有(C)2010,JPO&INPIT
    • 6. 发明专利
    • Detection method for allergen by immunochromatography method
    • 通过免疫印迹法测定过敏的检测方法
    • JP2007278773A
    • 2007-10-25
    • JP2006103678
    • 2006-04-04
    • Prima Meat Packers Ltdプリマハム株式会社
    • AKIMOTO MASANOBUKATO SHIGESHIRONAMIOKA MAKOTO
    • G01N33/543C07K16/16C07K16/18C07K17/12C07K17/14C12P21/08G01N33/53
    • PROBLEM TO BE SOLVED: To extract each allergen, by using SDS and 2-mercaptoethanol from a test sample, such as a food including each allergen, and to detect allergen quickly and accurately, by suppressing a nonspecific reaction resulting from decay of colloidal gold bonded to an antibody by SDS and 2-mercaptoethanol.
      SOLUTION: In this immunochromatographic method for detecting allergen by existence of accumulation of colloidal gold, after developing a developing solution on a developing support, by using a colloidal gold-labeled antibody formed, by bonding colloidal gold to a monoclonal antibody against denatured and native allergen, the developing support to which the monoclonal antibody recognizing epitope which is different from the colloidal gold-labeled antibody is fixed; a measuring sample of the denatured and native allergen extracted by using SDS and 2-mercaptoethanol, and the developing solution, the developing solution including at least 10 wt.% of fetal bovine serum (FBS) is used.
      COPYRIGHT: (C)2008,JPO&INPIT
    • 要解决的问题:通过使用来自测试样品的SDS和2-巯基乙醇,例如包括每种变应原的食物,并且通过抑制由于腐烂引起的非特异性反应,快速而准确地检测变应原来提取每种变应原 胶体金通过SDS和2-巯基乙醇与抗体结合。 解决方案:在通过存在胶体金的积累来检测变应原的免疫色谱法中,在显影载体上显影显影液后,通过使用形成的胶态金标记抗体,通过将胶体金与抗变性的单克隆抗体结合 和天然过敏原,与胶体金标记的抗体不同的单克隆抗体识别表位的显影载体是固定的; 使用SDS和2-巯基乙醇提取的变性和天然过敏原的测量样品和显影液,包含至少10重量%的胎牛血清(FBS)的显影溶液。 版权所有(C)2008,JPO&INPIT
    • 8. 发明专利
    • Novel stress biomarker and application thereof
    • 新型应力生物标记及其应用
    • JP2007225606A
    • 2007-09-06
    • JP2007018164
    • 2007-01-29
    • Prima Meat Packers LtdShizuoka Prefectureプリマハム株式会社静岡県
    • AKIMOTO MASANOBUNAMIOKA MAKOTONAKAYAMA TSUTOMUOHASHI NORIONAITO HIROYOSHI
    • G01N33/68G01N27/447G01N33/15G01N33/483G01N33/50G01N33/53
    • PROBLEM TO BE SOLVED: To provide a screening method, a stress determination method, and a stress diagnostic kit, for a stress restraint substance or a stress promoting substance, which use a novel stress biomarker. SOLUTION: A serum of a rat loaded with a stress by submerged restraint is subjected to two-dimensional electrophoresis. An electrophoretic image before loaded with the stress is compared with an electrophoretic image after loaded with the stress, and this screening method/stress determination method/kit of the present invention uses any protein selected from a group comprising apolipoprotein E, apolipoprotein A-IV, haptoglobin, and a vitamin-D coupled protein precursor, which are detected as two or more kinds of spots appearing in 5.0-6.2 of isoelectric point range after loaded with the stress, in 30kDa-60kDa of molecular weight range. COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供一种使用新型应力生物标志物的应力抑制物质或应激促进物质的筛选方法,应力测定方法和应力诊断试剂盒。 解决方案:通过浸没式约束装载应力的大鼠血清进行二维电泳。 加载应力后的电泳图像与加压后的电泳图像进行比较,本发明的筛选方法/应激测定方法/试剂盒使用选自载脂蛋白E,载脂蛋白A-IV, 触珠蛋白和维生素-D偶联蛋白前体,其分子量范围为30kDa-60kDa,被检测为在加载胁迫后出现在等电点范围的5.0-6.2中的两种或更多种斑点。 版权所有(C)2007,JPO&INPIT
    • 9. 发明专利
    • Novel stress biomarker and applications thereof
    • 新型应力生物标记及其应用
    • JP2007093597A
    • 2007-04-12
    • JP2006240039
    • 2006-09-05
    • Prima Meat Packers Ltdプリマハム株式会社
    • AKIMOTO MASANOBUNAMIOKA MAKOTOARIHARA KEIZOTOMITA KOHEIISHIKAWA SHINICHIITO MAKOTONAKAYAMA TSUTOMUOHASHI NORIOKATO AYAKO
    • G01N27/447
    • PROBLEM TO BE SOLVED: To provide a screening method of stress-inhibiting substance or stress-enhancing substance, a stress determination method, or a stress diagnostic kit that utilizes a novel stress biomarker. SOLUTION: The serum of a rat loaded with stress by 10-hour submersion constraint is tested by two-dimensional electrophoresis. Electrophoretic images of two-dimensional electrophoresis before and after stress loading are compared, and protein, such as creatine kinase isozyme (CK-MM) detected as four kinds of spots which appear at pH 6.0-7.0 and molecular weight 40-55 kD after stress loading, is used as a marker. The stomach is immediately extracted after blood collection, the area of ulcerated part is measured, and the ratio of the area of the ulcerated part to that of the stomach as a whole is taken as the ulcer index (%). The ulcer index of stress-related gastric ulcer by the submersion constraint test was about 20%. COPYRIGHT: (C)2007,JPO&INPIT
    • 要解决的问题:提供应激抑制物质或应激增强物质的筛选方法,应力测定方法或利用新型应激生物标志物的应激诊断试剂盒。 解决方案:通过二维电泳测试通过10小时浸没约束负载压力的大鼠血清。 比较应激负荷前后二维电泳的电泳图像,肌酸激酶同功酶(CK-MM)等蛋白质检测出4种斑点,出现在pH 6.0-7.0,应激后分子量40〜55 kD 加载,用作标记。 采集后立即抽出胃,测定溃疡部位的面积,将溃疡部位与胃部整体面积的比例作为溃疡指数(%)。 通过淹没约束试验,应激相关胃溃疡溃疡指数约为20%。 版权所有(C)2007,JPO&INPIT
    • 10. 发明专利
    • Method for detecting peanut allergen
    • 检测PEANUT过敏原的方法
    • JP2009271091A
    • 2009-11-19
    • JP2009191186
    • 2009-08-20
    • Prima Meat Packers Ltdプリマハム株式会社
    • AKIMOTO MASANOBUKATO SHIGESHIRONAMIOKA MAKOTO
    • G01N33/53C07K16/16C07K16/18C12N5/10C12N15/02G01N33/543G01N33/553G01N33/577G01N33/68
    • C07K16/16C07K16/18G01N33/5308G01N33/68G01N2333/4731Y10S530/85Y10S530/868
    • PROBLEM TO BE SOLVED: To provide a highly sensitive immunological detection method whereby an allergen (for example, milk allergen, egg albumen allergen, wheat allergen, buckwheat allergen or peanut allergen) contained in a food can be detected in any state (i.e., either denatured or undenatured), and to provide a detection kit to be used therein. SOLUTION: The method for detecting an allergen uses two or more monoclonal antibodies capable of recognizing milk allergen in the undenatured and denatured states, egg albumen allergen in the undenatured and denatured states, wheat allergen in the undenatured and denatured states, buckwheat allergen in the undenatured and denatured states, or peanut allergen in the undenatured and denatured states, wherein use is made of, as an indication, αs1αs1 casein which is the major protein in αs1 casein, β-lactoglobulin which is the major protein in whey, ovalbumin and ovomucoid which are the major proteins in egg albumen, gliadin which is the major protein in wheat, proteins of 24 kDa and 76 kDa in molecular weight which are the major proteins in buckwheat, or Ara h1 which is the major protein in peanut. COPYRIGHT: (C)2010,JPO&INPIT
    • 要解决的问题:为了提供高度敏感的免疫检测方法,其可以在任何状态下检测食物中包含的变应原(例如,牛奶变应原,蛋清变应原,小麦变应原,荞麦变应原或花生过敏原) 即变性或未变性),并提供其中使用的检测试剂盒。 解决方案:检测变应原的方法使用两种或更多种能够识别未变性和变性状态的牛奶过敏原的单克隆抗体,未变性和变性状态的蛋清变应原,未变性和变性状态的小麦变应原,荞麦变应原 在未变性和变性状态下,或未变性和变性状态的花生过敏原,其中使用作为αs1酪蛋白中主要蛋白质的αs1αs1酪蛋白,作为乳清中主要蛋白质的β-乳球蛋白,卵清蛋白 卵蛋白中的主要蛋白质,卵母细胞蛋白质,小麦中主要蛋白质的麦醇溶蛋白,作为荞麦主要蛋白质的分子量为24kDa和76kDa的蛋白质,或作为花生中主要蛋白质的Ara h1。 版权所有(C)2010,JPO&INPIT