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    • 2. 发明专利
    • Method and analyzer for fluorometric analyzer
    • 荧光分析仪的方法和分析仪
    • JP2004251814A
    • 2004-09-09
    • JP2003043936
    • 2003-02-21
    • Optoquest Co Ltd株式会社 オプトクエスト
    • TSUGITA HIROSHIOKUNO MASAFUMIWATABE AKIRA
    • G01N21/64G01N33/483G01N33/53G01N33/566G01N37/00
    • PROBLEM TO BE SOLVED: To enhance sensitivity and resolution in fluorometric measurement using a DNA chip, or a protein chip, making the best use of a basic technique for an existing femto-second confocal microscope.
      SOLUTION: A measuring system of high detection S/N ratio is realized, using two kinds of fluorescences different in fluorescence spectrum, in which the fluorescence spectra are crossed not in peak positions of the fluorescences but in wavelengths sided to foots thereof, and using as a light source for an excitation beam a femto-second pulse laser beam of which the wavelength is varied periodically around a crossing position as the center, and high-resolution of observation is attained using a two-photon or multiple-photon absorbing process.
      COPYRIGHT: (C)2004,JPO&NCIPI
    • 要解决的问题:为了提高使用DNA芯片或蛋白质芯片的荧光测量中的灵敏度和分辨率,充分利用现有的毫微微秒共聚焦显微镜的基本技术。 解决方案:实现了高检测S / N比的测量系统,使用荧光光谱不同的两种荧光,其中荧光光谱不在荧光物质的峰位置,而是波长偏离其脚部, 并且作为激发光束的光源使用波长以交叉位置为中心周期性变化的毫微微秒脉冲激光束,并且使用双光子或多光子吸收来获得高分辨率的观察 处理。 版权所有(C)2004,JPO&NCIPI