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1. 发明专利

JPH11341981A CHLAMYDOSPORE, ITS PRODUCTION, STARVATION MEDIUM USEFUL FOR PRODUCING CHLAMYDOSPORE AND USE OF CHLAMYDOSPORE 失效
公开(公告)号：JPH11341981A
公开(公告)日：1999-12-14
申请号：JP20387698
申请日：1998-07-17
申请人： OBIKEN KK , SASAKI YASUHARU
发明人： HORIUCHI ISAO
IPC分类号： C12N1/14 , C12N3/00 , C12R1/66 , C12R1/885
摘要： PROBLEM TO BE SOLVED: To produce a chlamydospore from a mycelial microorganism and to use the chlamydospore. SOLUTION: This chlamydospore 3 is characteristically obtained by subjecting a mycelial microorganism to starvation culture. The mycelial microorganism is cultured in a nutrient medium and a mycelium 2 and conidiospores 1 are separated from the medium. Then, a nutrient component attached to the separated mycelium 2 and conidiospores 1 is removed and the mycelium 2 and the conidiospores 1 from which the nutrient component is removed are subjected to starvation culture. A starvation medium comprises a silica gel as a main material, a small amount of organic substance fiber powder, a very small amount of Mg, Mn, Fe, Cu, Ni, Sn and Zn and a proper amount of water. After the mycelial microorganism is cultured in the nutrient medium, the mycelium 2 and the conidiospores 1 are removed from the nutrient medium, the nutrient component attached to the separated mycelium 2 and conidiospores 1 is removed and the mycelium 2 and the conidiospores 1 from which the nutrient component is removed are subjected to starvation culture in the starvation medium at 5-10 deg.C or 35-45 deg.C while dropping pH 7 to pH 4 or raising pH 7 to pH 9 for 24 to 240 hours to produce the objective chlamydospore 3.

2. 发明专利

JP2000093167A CHLAMYDOSPORE OF TRICHODERMA HARZIANUM, ITS PRODUCTION AND MICROBIAL MATERIAL 失效
公开(公告)号：JP2000093167A
公开(公告)日：2000-04-04
申请号：JP27108098
申请日：1998-09-25
申请人： OUBIKEN KK , SASAKI YASUHARU
发明人： HORIUCHI ISAO , KATAGIRI MIKIYUKI , SASAKI YASUHARU
IPC分类号： C12N3/00 , A01N63/02 , C12R1/885
摘要： PROBLEM TO BE SOLVED: To obtain a new chlamydospore of Trichoderma harzianum, usable as a microbial material or the like capable of suppressing pathogenic bacteria by readily germinating only by the impartment of water by removing nutrients from the mycelium of Trichoderma harzianum, culturing the obtained Trichoderma harzianum in a liquid starvation medium containing no vitamins, and separating the cultured material, and. SOLUTION: This new chlamydospore of Trichoderma harzianum obtained by removing nutrients from the mycelium obtained by culturing the Trichoderma harzianum, normally culturing the obtained Trichoderma harzianum in a liquid starvation medium containing no vitamins, and separating the cultured material, is useful as a microbial material or the like having activities capable of suppressing pathogenic bacteria by performing ready and active germination only by the impartment of water. The chlamydospore of the Trichoderma harzianum is obtained by culturing the Trichoderma harzianum in a nutrient medium, separating the mycelium from the nutrient medium, culturing the mycelium after removing the nutrient components in the liquid starvation medium, and separating the mycelium.

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