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    • 3. 发明专利
    • CHLAMYDOSPORE, ITS PRODUCTION, STARVATION MEDIUM USEFUL FOR PRODUCING CHLAMYDOSPORE AND USE OF CHLAMYDOSPORE
    • JPH11341981A
    • 1999-12-14
    • JP20387698
    • 1998-07-17
    • OBIKEN KKSASAKI YASUHARU
    • HORIUCHI ISAO
    • C12N1/14C12N3/00C12R1/66C12R1/885
    • PROBLEM TO BE SOLVED: To produce a chlamydospore from a mycelial microorganism and to use the chlamydospore. SOLUTION: This chlamydospore 3 is characteristically obtained by subjecting a mycelial microorganism to starvation culture. The mycelial microorganism is cultured in a nutrient medium and a mycelium 2 and conidiospores 1 are separated from the medium. Then, a nutrient component attached to the separated mycelium 2 and conidiospores 1 is removed and the mycelium 2 and the conidiospores 1 from which the nutrient component is removed are subjected to starvation culture. A starvation medium comprises a silica gel as a main material, a small amount of organic substance fiber powder, a very small amount of Mg, Mn, Fe, Cu, Ni, Sn and Zn and a proper amount of water. After the mycelial microorganism is cultured in the nutrient medium, the mycelium 2 and the conidiospores 1 are removed from the nutrient medium, the nutrient component attached to the separated mycelium 2 and conidiospores 1 is removed and the mycelium 2 and the conidiospores 1 from which the nutrient component is removed are subjected to starvation culture in the starvation medium at 5-10 deg.C or 35-45 deg.C while dropping pH 7 to pH 4 or raising pH 7 to pH 9 for 24 to 240 hours to produce the objective chlamydospore 3.