专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2013005791A Rrhj1ii nuclease and gene of the same 有权
标题翻译： RRHJ1II核仁及其基因
公开(公告)号：JP2013005791A
公开(公告)日：2013-01-10
申请号：JP2012038067
申请日：2012-02-24
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , WATANABE FUMIAKI , KITA KEIKO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/16 , C12N9/22
摘要： PROBLEM TO BE SOLVED: To provide a new nuclease and a gene of the same useful in the field of a genetic engineering, and to provide a production method of the nuclease using the same.SOLUTION: The isolation of a new nuclease that has a nicking enzyme activity from a Rhodococcusrhodochrous J-1 strain, the determination of an amino acid sequence of the enzyme, and the determinations of the base sequence of a gene that encodes the same are disclosed. The production method of the RrhJ1II nuclease by a recombinant bacterium that expresses the new nuclease gene is disclosed.
摘要翻译：要解决的问题：提供在遗传工程领域中有用的新的核酸酶和基因，并提供使用其的核酸酶的制备方法。解决方案：从Rhodococcusrhodochrous J-1菌株分离具有切口酶活性的新核酸酶，测定酶的氨基酸序列以及编码该基因的碱基序列的测定被披露。公开了通过表达新核酸酶基因的重组细菌生产RrhJ1II核酸酶的方法。版权所有（C）2013，JPO＆INPIT

2. 发明专利

JP2013005792A Rhodococcus bacillus in which nuclease gene made to be deleted or inert 有权
标题翻译：其中核苷酸基因的脱氧核糖核酸
公开(公告)号：JP2013005792A
公开(公告)日：2013-01-10
申请号：JP2012062458
申请日：2012-03-19
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , WATANABE FUMIAKI , KITA KEIKO
IPC分类号： C12N15/09 , C12N1/21
摘要： PROBLEM TO BE SOLVED: To provide Rhodococcus bacillus in which the physical strength is strong, and which has the capacity that accumulates an enzyme or the like in the cell a lot or like, thereby which is known as a useful a microbial catalyst useful for industry, and is used for the enzymic hydration of nitriles or the production of an amide or an acid by the hydrolysis or the like, and in which the transformation efficiency is excellent.SOLUTION: A gene deletion host in which at least one or more of the nuclease genes that the Rhodococcus bacillus has is made to be deleted or inert is disclosed.
摘要翻译：待解决的问题：为了提供物理强度强的具有能够在细胞中积聚酶等的能力的红球菌属菌种，其被称为有用的微生物催化剂对于工业有用，用于通过水解等进行腈水解或酰胺或酸的生成，转化效率优异。解决方案：公开了一种基因缺失宿主，其中已将至少一种或多种具有红球菌属的核酸酶基因制成缺失或惰性的宿主。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2012228221A Improved halohydrin epoxidase 审中-公开
标题翻译：改善的氢化环氧化酶
公开(公告)号：JP2012228221A
公开(公告)日：2012-11-22
申请号：JP2011099619
申请日：2011-04-27
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , WATANABE FUMIAKI , TO FUJIO , KAIODA MASABUMI , OTAKA MASAFUMI , OTAKI AKASHI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/88 , C12P7/00 , C12P13/02
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide an improved halohydrin epoxidase in which stereoselectivity, product inhibition resistance, and the like are improved, and to provide a method, by using the same, for efficiently producing optically active epihalohydrin or 4-halo-3-hydroxybutyronitrile by using them.SOLUTION: In the amino acid sequence of a wild type halohydrin epoxidase shown by a specific sequence, an improved halohydrin epoxidase consisting of the amino acid sequence into which the amino acid mutations substituting Ala at the second position with Lys, and also Ala at the 128th position with Cys, Gln, Ile, Met or Val are introduced is used.
摘要翻译：待解决的问题：提供其中立体选择性，产品抑制性等改善的改进的卤代醇环氧酶，并且提供一种通过使用它们有效地制备光学活性表卤代醇或4-卤代 - 3-羟基丁腈。解决方案：在特定序列所示的野生型卤代醇环氧酶的氨基酸序列中，由氨基酸序列组成的改进的卤代醇环氧酶，其中用Lys代替第二位的Ala的氨基酸突变，以及Ala 使用Cys，Gln，Ile，Met或Val的第128位。版权所有（C）2013，JPO＆INPIT

4. 发明专利

JP2012090537A Improved type halohydrin epoxidase 审中-公开
标题翻译：改进的类型HALOHYDRIN环氧化酶
公开(公告)号：JP2012090537A
公开(公告)日：2012-05-17
申请号：JP2010238762
申请日：2010-10-25
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , TO FUJIO , AKIYAMA TAKAMICHI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/88 , C12P13/00 , C12P17/02
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To obtain improved type halohydrin epoxidases in which stereoselectivity, product inhibition resistance, etc., are improved, and provide a method for efficiently producing an optically active epihalohydrin or a 4-halo-3-hydroxybutyronitrile by utilizing them.SOLUTION: In the amino acid sequence of a wild type halohydrin epoxidase consisting of a specific amino acid sequence, the improved type halohydrin epoxidases of which second Ala is substituted with Lys and introducing at least one kind of amino acid mutation selected from the following (i) to (l), are provided. (i) The amino acid mutation in which 29th Glu is substituted with Lys, (j) the amino acid mutation in which 179th Glu is substituted with Gln, (k) the amino acid mutation in which both 183th Asp and 184th Met are substituted with Val, and (l) the amino acid mutation in which 221th Phe is substituted with Leu.
摘要翻译：待解决的问题：为了获得立体选择性，产品抑制阻力等改进型改性型卤代醇环氧化酶，并且提供了一种通过利用光学活性表卤代醇或4-卤代-3-羟基丁腈有效生产光学活性表卤代醇的方法他们。解决方案：在由特定氨基酸序列组成的野生型卤代醇环氧酶的氨基酸序列中，改进型卤代醇环氧化酶，其中第二个Ala被Lys取代，并引入至少一种氨基酸突变，其选自提供以下（i）至（l）。（i）其中第29位Glu被Lys取代的氨基酸突变，（j）第179位Glu被Gln取代的氨基酸突变，（k）第183位Asp和第184位Met取代的氨基酸突变 Val和（l）第221位Phe被Leu取代的氨基酸突变。版权所有（C）2012，JPO＆INPIT

5. 发明专利

JP2013009621A Method for producing optically-active 4-halo-3-hydroxybutyronitrile 审中-公开
标题翻译：用于生产光学活性的4-羟基-3-羟基丁酸的方法
公开(公告)号：JP2013009621A
公开(公告)日：2013-01-17
申请号：JP2011143895
申请日：2011-06-29
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , WATANABE TAKESHI , WATANABE FUMIAKI , SATO EIJI , NINOMIYA YASUHIRO
IPC分类号： C12P13/00 , C12N9/14 , C12N15/09
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide a method for producing 4-halo-3-hydroxybutyronitrile of high concentration and high optical purity.SOLUTION: There is provided the method for producing 4-halo-3-hydroxybutyronitrile from a 1, 3-dihalo-2-propanol and a cyanide donor by enzyme reaction. After the initiation of the enzyme reaction, the 1, 3-dihalo-2-propanol is supplied to a reaction liquid and the concentration of the 1, 3-dihalo-2-propanol is held in the range of not more than 0.43 mol/kg.
摘要翻译：待解决的问题：提供一种高浓度和高光学纯度的4-卤代-3-羟基丁腈的制备方法。解决方案：提供了通过酶反应从1,3-二卤代-2-丙醇和氰化物供体产生4-卤-3-羟基丁腈的方法。酶反应开始后，将1,3-二卤代-2-丙醇供给到反应液中，1,3-二卤代-2-丙醇的浓度保持在不大于0.43mol / 公斤。版权所有（C）2013，JPO＆INPIT

6. 发明专利

JP2011205921A Recombinant rhodococcus bacterium and method for producing optically active (r)-3-quinuclidinol by using the same 审中-公开
标题翻译：重组RHODOCOCCUS细菌及其使用方法生产光学活性（R）-3-喹啉啶醇
公开(公告)号：JP2011205921A
公开(公告)日：2011-10-20
申请号：JP2010074739
申请日：2010-03-29
申请人： Mitsubishi Rayon Co Ltd , 三菱レイヨン株式会社
发明人： KUWAHARA FUMI , MIZUNASHI WATARU
IPC分类号： C12N1/21 , C07D453/02 , C12N15/09 , C12P17/18 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a method for continuously producing optically active (R)-3-quinuclidinol.SOLUTION: There are disclosed a recombinant vector comprising a 3-quinuclidinone asymmetric reductase gene and a coenzyme-regenerative enzyme gene, a gene recombinant in which the vector is introduced, and a method for producing optically active (R)-3-quinuclidinol by using the recombinant.
摘要翻译：要解决的问题：提供连续制造光学活性（R）-3-奎宁环醇的方法。溶液：公开了包含3-奎宁环酮不对称还原酶基因和辅酶再生酶基因的重组载体，其导入载体，以及通过使用重组体制备光学活性（R）-3-奎宁环醇的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式