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1. 发明专利

JP2007228819A Offensive/musty odor-low-productive koji mold 审中-公开
标题翻译：有风味/必须气味低的KOJI模具
公开(公告)号：JP2007228819A
公开(公告)日：2007-09-13
申请号：JP2006051520
申请日：2006-02-28
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： TAKECHI JUNYA , HARA SEIICHI , HANYA YOSHISATO , KOYAMA TAIJI
IPC分类号： C12N1/15 , A23L5/20 , A23L27/50 , C12N15/09 , C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide well-flavored high-quality food/drink slight in offensive and stuffy odor. SOLUTION: A koji mold is provided, which is produced by a gene manipulation such as gene disruption and in which a branched-chain amino acid aminotransferase gene or branched-chain α-keto-acid dehydrogenase gene does not function. A method for screening the above koji mold is also provided, being based on the phenotype of a koji mold in which the above gene is deleted, or comprising assaying the activity of the above-mentioned enzyme. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供味道好的高品质食品/饮料，有点冒险和闷热的气味。提供了通过诸如基因破坏等基因操作产生的曲线霉菌，其中支链氨基酸氨基转移酶基因或支链α-酮酸脱氢酶基因不起作用。还提供了用于筛选上述曲霉的方法，其基于上述基因缺失的曲霉的表型，或包括测定上述酶的活性。版权所有（C）2007，JPO＆INPIT

2. 发明专利

JP2009095279A Koji mold, method for breeding the same, and method for producing soy sauce 审中-公开
标题翻译： KOJI模具，其育种方法以及生产大豆的方法
公开(公告)号：JP2009095279A
公开(公告)日：2009-05-07
申请号：JP2007269954
申请日：2007-10-17
申请人： Japan Atomic Energy Agency , Kikkoman Corp , キッコーマン株式会社 , 独立行政法人日本原子力研究開発機構
发明人： HANYA YOSHISATO , KOYAMA TAIJI , ITO KOTARO , TAKECHI JUNYA , OTSUKA TERUO , NARUMI KAZUNARI , SATO KATSUYA , HASE SUMIHIRO , KOBAYASHI YASUHIKO , SAKASHITA TETSUYA
IPC分类号： C12N1/14 , A23L27/50
摘要： PROBLEM TO BE SOLVED: To breed a Koji mold having the protease productivity of ≥1,100 U/g Koji and the glutaminase productivity of ≥2.0 U/g Koji, and to provide soy sauce having a high nitrogen utilization ratio, total nitrogen and glutamic acid content using the Koji mold. SOLUTION: The Koji mold is obtained by irradiating a strain belonging to the genus Aspergillus with a heavy ion beam and separating the Koji mold having protease productivity of ≥1,100 U/g Koji and glutaminase productivity of ≥2.0 U/g Koji from the irradiated strain or a cultured product thereof. The resultant Koji mold is inoculated into raw materials for soy sauce Koji and cultured to prepare the Koji, and the resultant Koji is used to carry out preparation for brewing. As a result, the soy sauce is obtained. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：为了培育蛋白酶生产率≥1,100U/ g Koji和谷氨酰胺酶生产率≥2.0U/ g Koji的Koji模具，并提供具有高氮利用率的酱油，总氮和谷氨酸含量使用Koji模具。解决方案：通过用重离子束照射属于曲霉属的菌株并将蛋白酶生产率≥1,100U/ g Koji的Koji模具和≥2.0U/ g Koji的谷氨酰胺酶生产率从照射的菌株或其培养产物。将所得的Koji模具接种到酱油Koji的原料中并培养以制备Koji，并且所得的Koji用于进行酿造的准备。结果，得到酱油。版权所有（C）2009，JPO＆INPIT

3. 发明专利

JP2010246481A Aspergillus oryzae having large-scale genome duplication 有权
标题翻译：具有大规模基因组重复的ASPERGILLUS ORYZAE
公开(公告)号：JP2010246481A
公开(公告)日：2010-11-04
申请号：JP2009100645
申请日：2009-04-17
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： SATO ATSUSHI , HANYA YOSHISATO
IPC分类号： A23L27/24 , C12N1/14 , A23L27/21 , A23L27/50 , C12N15/09
CPC分类号： A23L1/238 , A23L2/84 , A23L27/50 , C12N9/242 , C12N9/58 , C12N9/62 , C12N15/52 , C12R1/66 , C12R1/69 , C12Y302/01001
摘要： PROBLEM TO BE SOLVED: To breed Aspergillus oryzae having high productivity of various enzymes responding to industries without using a gene recombination technique. SOLUTION: The Aspergillus oryzae is a strain belonging to the genus Aspergillus, has a large-scale genome duplication of ≥900 kb and highly produces a group of various kinds of enzymes such as protease, necessary for production of soy sauce. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：在不使用基因重组技术的情况下培养具有高反应性的各种酶的各种酶的高生产力的米曲霉。解决方案：米曲霉属于曲霉属的菌株，具有≥9900kb的大规模基因组重复，并且高产生生产酱油所必需的各种各样的酶如蛋白酶。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2005080541A Method for producing solid koji 审中-公开
标题翻译：生产固体KOJI的方法
公开(公告)号：JP2005080541A
公开(公告)日：2005-03-31
申请号：JP2003314875
申请日：2003-09-08
申请人： Kikkoman Corp , キッコーマン株式会社
发明人： SHINTSU KENJI , HANYA YOSHISATO , SOMEYA HIDEYUKI , YAMADA MUNEKI
IPC分类号： C12N1/14
摘要： PROBLEM TO BE SOLVED: To provide a method for producing solid koji having such advantages that a koji stock is easily packed in a culture vessel, the opening of the culture vessel is easily sealed and the koji stock is easily inoculated with koji mould, the culture vessel can be used repeatedly, therefore being suitable for the mass production of the solid koji, the koji stock can be prevented from being stuck on the inner circumferential surface of the culture vessel, the inner circumferential surface of the culture vessel can be washed with water and washed off water very easily, and even if stuck, koji can be easily peeled off and recovered from the inner circumferential surface of the culture vessel in unloading the koji, and the objective solid koji contaminated with few sundry germs can be obtained easily without caring for.
SOLUTION: The solid koji is obtained by the following procedure: A heat-resistant and hydrophobic air-permeable nonwoven fabric is laid on a box-shaped vessel with the top open and both air-permeable bottom and circumferential sidewall, the koji stock is packed on the nonwoven fabric, the margin of the nonwoven fabric is folded back toward the center, these box-shaped vessels are stacked in tiers, the top surface and the underside of these vessels are provided with spaces for releasing heat generated by culture respectively, and the koji stock is cultured without caring for.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：为了提供一种生产固体曲的方法，其具有将曲曲原料容易地包装在培养容器中的优点，培养容器的开放容易密封，并且曲曲原料容易地用曲霉菌可以重复使用培养容器，因此适合于大量生产固体曲，可以防止曲柄原料粘在培养容器的内周面上，培养容器的内周面可以是用水洗涤，非常容易地清洗，即使卡住，也可以在将曲子卸载时，从培养容器的内周面容易地剥离和回收曲，并且可以获得被少量各种细菌污染的目标实心曲轻松无忧。解决方案：通过以下步骤获得固体曲：将耐热和疏水的透气性无纺布铺设在具有顶部开口和透气性底部和周向侧壁的盒形容器上，该曲线将原料包装在无纺布上，无纺布的边缘向中心折回，这些箱形容器层叠，这些容器的上表面和下侧设置有用于释放由培养产生的热量的空间分别培养了曲靖股份，无需照顾。版权所有（C）2005，JPO＆NCIPI

5. 发明专利

JP2012187102A Novel protease gene, recombinant dna, and method for making protease 审中-公开
标题翻译：新型蛋白酶基因，重组DNA和制备蛋白质的方法
公开(公告)号：JP2012187102A
公开(公告)日：2012-10-04
申请号：JP2012039230
申请日：2012-02-24
申请人： Kikkoman Corp , Noda Institute For Scientific Research , キッコーマン株式会社 , 公益財団法人野田産業科学研究所
发明人： OGAWA SHINKO , SATO ATSUSHI , TAKAHASHI OSAMU , OSHIMA KENSHIRO , HATTORI SHOHEI , KOYAMA TAIJI , OGUMA TETSUYA , HANYA YOSHISATO
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/62
摘要： PROBLEM TO BE SOLVED: To provide: a novel protease capable of decomposing a protein present in a raw material such as a soy sauce and an enzyme decomposing seasoning; a novel gene encoding the protease; a recombinant DNA including the gene; a transformant or a transductant including the recombinant DNA; and a method for making the protease using the transformant and the transductant.SOLUTION: The method for making the protease is characterized by: culturing the proteins having protease activity including a specific amino acid sequence or others, a gene encoding the protein or others, the recombinant vector including the gene, the transformant including the recombinant vector, and the transformant; and recovering the protease from the obtained cultured substance.
摘要翻译：待解决的问题：提供：能够分解存在于原料如酱油和酶分解调味料中的蛋白质的新型蛋白酶; 编码蛋白酶的新基因; 包含该基因的重组DNA; 包含重组DNA的转化体或转导体; 以及使用转化体和转导体制造蛋白酶的方法。解决方案：制备蛋白酶的方法的特征在于：培养具有蛋白酶活性的蛋白酶活性，包括特定氨基酸序列等，编码蛋白质的基因等，包含该基因的重组载体，转化体包括重组体载体和转化体; 从所得培养物中回收蛋白酶。版权所有（C）2013，JPO＆INPIT

6. 发明专利

JP2009095280A Method for breeding mutant strain of yellow koji mold 审中-公开
标题翻译：育种黄曲霉的突变菌的方法
公开(公告)号：JP2009095280A
公开(公告)日：2009-05-07
申请号：JP2007269956
申请日：2007-10-17
申请人： Japan Atomic Energy Agency , Kikkoman Corp , キッコーマン株式会社 , 独立行政法人日本原子力研究開発機構
发明人： HANYA YOSHISATO , KOYAMA TAIJI , ITO KOTARO , TAKECHI JUNYA , OTSUKA TERUO , NARUMI KAZUNARI , SATO KATSUYA , KOBAYASHI YASUHIKO , SAKASHITA TETSUYA , HASE SUMIHIRO
IPC分类号： C12N1/14
摘要： PROBLEM TO BE SOLVED: To provide a mutant strain of yellow Koji mold which has less accompanying mutation and could not be obtained by a conventional mutation method, e.g. a Koji mold having protease productivity of ≥1,100 U/g Koji and glutaminase productivity of ≥2.0 U/g Koji, and to provide a gene-deficient mutant strain (pyrG mutant strain) etc.
SOLUTION: The yellow Koji mold such as Aspergillus soya and Aspergillus oryzae is irradiated with a heavy ion beam and the yellow Koji mold causing a mutation is selected from among the irradiated material and a cultured product thereof to thereby afford the mutant strain of the yellow Koji mold having desired microbiological characteristics.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种具有较少伴随突变且不能通过常规突变方法获得的黄色Koji霉菌的突变菌株，例如，蛋白酶生产率≥1,100U / g Koji，谷氨酰胺酶生产力≥2.0U / g Koji的Koji模具，并提供基因缺陷型突变株（pyrG突变株）等。解决方案：黄色Koji模具作为曲霉大豆和米曲霉用重离子束照射，从被照射材料及其培养物中选择引起突变的黄色Koji模具，由此提供具有所需微生物特征的黄色Koji模具的突变株。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2003088356A VESSEL FOR CULTURING SOLID RICE HALT AND METHOD OF PRODUCING RICE MALT USING THE SAME 审中-公开
公开(公告)号：JP2003088356A
公开(公告)日：2003-03-25
申请号：JP2001282551
申请日：2001-09-18
申请人： KIKKOMAN CORP
发明人： FURUKAWA TOSHIO , SHINTSU KENJI , HANYA YOSHISATO , MATSUNO YOSHIHIKO , SOMEYA HIDEYUKI
IPC分类号： C12M1/16 , C12C1/033 , C12N1/14
摘要： PROBLEM TO BE SOLVED: To provide a simple method of producing solid Koji (rice malt) freed of contamination with various microorganisms. SOLUTION: A culture vessel is porous, resistant to heat and lidded. The raw materials for making Koji are sterilized, steamed and cooled in the culture vessel. Then, the resultant raw materials in the vessel is inoculated in the germ- free chamber, then cultured in the culture chamber.

8. 发明专利

JP2000152776A PRODUCTION OF KOJI AND ROTARY CYLINDRICAL APPARATUS THEREFOR 失效
公开(公告)号：JP2000152776A
公开(公告)日：2000-06-06
申请号：JP32959198
申请日：1998-11-19
申请人： KIKKOMAN CORP
发明人： FURUKAWA TOSHIO , SHINTSU KENJI , HANYA YOSHISATO , OKAMOTO YOSHIHARU
IPC分类号： C12M1/16 , C12N1/14 , C12R1/69
摘要： PROBLEM TO BE SOLVED: To provide a method and apparatus for culturing a solid medium in a sterile condition. SOLUTION: This apparatus, a rotary cylindrical culturing apparatus 2, is made up of a cylindrical culturing vessel 4, a covering member 10 consisting of a microfilter 13, provided with a seed koji charge port 17 and covering the side face of the culturing vessel 4, and a rotary driving unit 5 functioning to rotate the culturing vessel 4. Using this apparatus, a solid medium is subjected to sterile culture.

9. 发明专利

JPH11127812A PRODUCTION OF SEASONING 失效
公开(公告)号：JPH11127812A
公开(公告)日：1999-05-18
申请号：JP31108597
申请日：1997-10-28
申请人： KIKKOMAN CORP
发明人： SUGISHITA MISAO , FUKUSHIMA YAICHI , HANYA YOSHISATO , NAKADAI TADANOBU
IPC分类号： A23L11/20 , A23L27/00 , A23L27/24 , A23L1/22 , A23L1/202 , A23L1/23
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a seasoning having excellent flavor and color and luster stability, by efficiently hydrolyzing a protein raw material. SOLUTION: In this method for producing a seasoning by hydrolyzing a protein raw material such as defatted soybean meal, an enzyme such as a liquid koji and a yeast are added to the raw material and the raw material is fermented while being hydrolyzed. Then the fermented material is pressed and filtered by a route procedure. Preferably the hydrolysis and the fermentation are carried out at 37-45 deg.C.

10. 发明专利

JPH04330273A NEW MUTANT STRAIN AND PREPARATION OF SOY SAUCE HAVING HIGH AMINO ACID CONTENT USING SAID STRAIN 失效
公开(公告)号：JPH04330273A
公开(公告)日：1992-11-18
申请号：JP12441291
申请日：1991-04-30
申请人： KIKKOMAN CORP
发明人： AOKI MITSUTATSU , HANYA YOSHISATO , UCHIDA KINJI
IPC分类号： A23L27/50 , C12N1/16 , C12R1/645
摘要： PURPOSE:To provide a new mutant strain capable of taking and utilizing ammonia as nitrogen source in the growth and proliferation in unrefined soy sauce and scarcely taking amino acids existing in the sauce as taste substances and useful for the fermentation and ripening of soy sauce to obtain a soy sauce having excellent taste. CONSTITUTION:The objective mutant strain is a microorganism belonging to the genus Zygosaccharomyces, resistant to p-fluoro-DL-phenylalanine and having low activity to take amino acid, e.g. Zygosaccharomyces FPA-16 (FERM P-12221). The Zygosaccharomyces FPA-16 can be produced by using Zygosaccharomyces rouxii ATCC 13356 as parent strain and subjecting to conventional mutagenic treatment such as ultraviolet irradiation.

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