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1. 发明专利

JP2008259453A Method for detecting nucleic acid 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：JP2008259453A
公开(公告)日：2008-10-30
申请号：JP2007104938
申请日：2007-04-12
申请人： Institute Of Physical & Chemical Research , Toyobo Co Ltd , 東洋紡績株式会社 , 独立行政法人理化学研究所
发明人： ABE HIROSHI , ITO YOSHIHIRO , FURUKAWA KAZUHIRO , SHIUCHI KEIICHI , SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78 , G01N33/53 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a nucleic acid quickly and accurately, and a kit for the same.
SOLUTION: This method for detecting the nucleic acid is provided by mixing a nucleic acid probe set containing a first nucleic acid probe and second nucleic acid probe each having a sequence complementary to that of the objective nucleic acid, with a sample containing the objective nucleic acid to develop a non-enzymatic bonding reaction, and the kit for the same is also provided.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供快速准确地检测核酸的方法及其用途。解决方案：用于检测核酸的方法通过将含有第一核酸探针的核酸探针组和各自具有与目的核酸的序列互补的序列的第二核酸探针与含有还提供了用于开发非酶结合反应的客观核酸，以及用于其的试剂盒。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2006020516A Method for identifying base polymorphism 审中-公开
标题翻译：识别基因多态性的方法
公开(公告)号：JP2006020516A
公开(公告)日：2006-01-26
申请号：JP2004198913
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting base polymorphism clearly and simply, capable of obtaining a result rapidly and easily in a good reproducibility, since without requiring complex operations.
SOLUTION: This method for detecting the base polymorphism by performing an amplifying reaction by using an oligonucleotide in a specimen solution containing a specific nucleic acid sequence and at the same time with or after the amplification reaction, bringing the oligonucleotide bonded with a first ligand specifically bonding with the nucleic acid sequence in contact with an oligonucleotide specifically recognizing the base polymorphic zone bonded with a second ligand is provided by using a solid carrier bonded with a capturing agent bonding with the first ligand and a physiologically active substance bonding with the second ligand.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供一种清楚简单地检测碱基多态性的方法，能够以良好的再现性快速且容易地获得结果，因为不需要复杂的操作。解决方案：该方法通过在含有特定核酸序列的样品溶液中进行扩增反应并与扩增反应同时或之后进行扩增反应来检测碱基多态性，使寡核苷酸与第一通过使用与与第一配体结合的捕获剂键合的固体载体和与第二配体键合的生理活性物质来提供与与特异性识别与第二配体结合的碱基多态性区域的寡核苷酸接触的核酸序列特异性结合的配体配体。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2006020509A Method for detecting nucleic acid 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：JP2006020509A
公开(公告)日：2006-01-26
申请号：JP2004198906
申请日：2004-07-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/50
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a nucleic acid sequence in a specimen quickly, easily and in a good reproducibility. SOLUTION: This method for detecting the nucleic acid sequence by performing an amplifying reaction by using an amplifying oligonucleotide bonded with a first ligand in a specimen solution containing the specific nucleic acid sequence and bringing a bonded material bonded with a second ligand specifically capable of bonding with the nucleic acid sequence in contact with the above at the same time with the amplification reaction or after the reaction and detecting a complex bonded with a bonded second ligand comprises a process of bonding the second ligand part of the complex on the surface of a liquid-passing filter bonded with a second ligand-capturing agent, a process of dripping a solution of a labeled physiologically active substance capable of bonding with the first ligand for bonding the labeled physiologically active substance with the first ligand and a process for titrating the label bonded with the liquid-passing filter. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种快速，容易且以良好再现性检测样品中核酸序列的方法。解决方案：该方法通过在含有特定核酸序列的试样溶液中使用与第一配体结合的扩增寡核苷酸进行扩增反应并使与第二配位体特异性结合的键合材料进行扩增反应来检测核酸序列与与上述接触的核酸序列在与扩增反应同时或在反应之后或检测与键合的第二配体结合的复合物的键合包括将复合物的第二配体部分键合到表面上的方法与第二配体捕获剂结合的液体通过过滤器，将能够与第一配体结合的标记的生理活性物质的溶液与第一配位体结合的方法和滴定标记的生理活性物质的方法标签与液体通过过滤器粘合。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2005287499A Method for identifying base polymorphism 有权
标题翻译：识别基因多态性的方法
公开(公告)号：JP2005287499A
公开(公告)日：2005-10-20
申请号：JP2004293565
申请日：2004-10-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method by which the polymorphism in a nucleic acid sequence can be detected clearly and in good repeatability; and to obtain a reagent therefor.
SOLUTION: The base polymorphism is identified by simultaneously or separately reacting a nucleic acid sequence containing a specific base polymorphism site contained in a sample, with an oligonucleotide for detecting a wild type in which at least one base in bases from the 3' terminus to the fifth base is substituted with a substituent not having interaction with a DNA-type base or an RNA-type base, and one or more kinds of oligonucleotides for detecting a variant. The substituent not having interaction with the DNA-type base or the RNA-type base is preferably inosine.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供可以清楚地且重复性良好地检测核酸序列中的多态性的方法; 并获得试剂。解决方案：通过同时或单独地将含有样品中含有的特定碱基多态性位点的核酸序列与用于检测野生型的寡核苷酸同时或分别反应来鉴定碱基多态性，其中至少一个碱基从3' 末端至第五碱基被不与DNA型碱基或RNA型碱基相互作用的取代基取代，以及一种或多种用于检测变体的寡核苷酸。不与DNA型碱基或RNA型碱基相互作用的取代基优选为肌苷。版权所有（C）2006，JPO＆NCIPI

5. 发明专利

JP2005245272A Simple method for detecting genetic polymorphism of alcohol dehydrogenase, and reagent for detection 审中-公开
标题翻译：用于检测酒精脱氢酶的遗传多态性的简单方法和用于检测的试剂
公开(公告)号：JP2005245272A
公开(公告)日：2005-09-15
申请号：JP2004058810
申请日：2004-03-03
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： YOSHIGA SATOKO , SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for clearly detecting a genetic polymorphism of an alcohol dehydrogenase in good repeatability; and to provide a reagent therefor. SOLUTION: The method for detecting the genetic polymorphism of the alcohol dehydrogenase involves carrying out the amplification by using at least one primer hybridizable with a sequence of continuous 15 bases or a base sequence complementary to the sequence in specific sequences specifically hybridizable with an alcohol hydrogenase gene in the method for detecting the sequence of a varied position of the alcohol dehydrogenase gene. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种以良好的重复性清楚地检测醇脱氢酶的遗传多态性的方法; 并提供试剂。检测醇脱氢酶的遗传多态性的方法涉及通过使用至少一种能与连续15个碱基序列杂交的引物或与该序列互补的碱基序列进行扩增的特异性序列，其特异性可与醇氢化酶基因检测醇脱氢酶基因不同位置序列的方法。版权所有（C）2005，JPO＆NCIPI

6. 发明专利

JP2011092143A Method for measuring bicarbonate concentration and solution used for the same 有权
标题翻译：用于测量双硫酸浓度的方法和用于其的溶液
公开(公告)号：JP2011092143A
公开(公告)日：2011-05-12
申请号：JP2009251380
申请日：2009-10-30
申请人： Mie Univ , Toyobo Co Ltd , 国立大学法人三重大学 , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , NOBORI TSUTOMU , NAKATANI ATARU , NOMURA SHINSUKE , TERAMURA YOSHIKAZU
IPC分类号： C12Q1/25 , C12Q1/32 , G01N33/84
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring a bicarbonate concentration and a solution (reagent) used for the same in which a measuring performance is not deteriorated even while the reagent for measurements remains exposed to an atmosphere for a long time.
SOLUTION: The method for measuring bicarbonate concentration including the steps of: (A) reacting bicarbonate ions and phosphoenolpyruvic acid in a reagent under phosphoenolpyruvic acid carboxylase presence to produce oxaloacetic acid; (B) reacting the oxaloacetic acid produced in the step (A) and NADH or its analog under maleic acid dehydrogenase presence; (C) and measuring a decrease of NADH or its analog, wherein the step (A) is performed in a solution of pH7 or more and less than pH8, the ratio of a front area of the solution in contact with the atmosphere to a volume of the solution is less than 0.2, and phosphoenolpyruvic acid carboxylase is acetic acid bacteria based phosphoenolpyruvic acid carboxylase.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：为了提供一种用于测量碳酸氢盐浓度的方法和用于测量性能即使在长时间暴露于大气中的测量性能也不劣化的溶液（试剂）的方法。解决方案：测量碳酸氢盐浓度的方法，包括以下步骤：（A）在磷酸烯醇丙酮酸羧化酶存在下使碳酸氢根离子和磷酸烯醇式丙酮酸反应生成草酰乙酸; （B）在马来酸脱氢酶存在下使步骤（A）中产生的草酰乙酸与NADH或其类似物反应; （C）并测量NADH或其类似物的降低，其中步骤（A）在pH7以上且小于pH8的溶液中进行，所述溶液与大气接触的前面积与体积的溶液小于0.2，而磷酸烯醇丙酮酸羧化酶是基于乙酸菌的磷酸烯醇丙酮酸羧化酶。版权所有（C）2011，JPO＆INPIT

7. 发明专利

JP2011027731A Method for sucking reagent 审中-公开
标题翻译：消除试剂的方法
公开(公告)号：JP2011027731A
公开(公告)日：2011-02-10
申请号：JP2010147397
申请日：2010-06-29
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： MISAWA SHUHEI , WAKAHARA TAKASHI , SOYA YOSHIHIRO
IPC分类号： G01N35/10 , G01N1/00
摘要： PROBLEM TO BE SOLVED: To provide a method for sucking a reagent from a reagent cartridge for use in a physiologically active material measurement system, from a point of view of providing such a system in conformity with the idea of POCT. SOLUTION: Before divided injection or discharge of a reagent, a seal is bored through with a syringe or the like in advance so that a hole is made through which air flows into a container in place of a reagent that is sucked in or through which air flows out of a container in place of a reagent that is discharged. Then, by sucking or discharging the reagent by inserting a syringe into a position different from the hole for suction or discharge, stable suction or discharge is carried out, so that stable measured values are obtained. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：从提供符合POCT的想法的观点出发，提供从用于生理活性物质测量系统的试剂盒吸取试剂的方法。解决方案：在试剂分开注射或排放之前，预先用注射器等将密封件穿孔，以便空气流入容器中而不是吸入的试剂，空气从容器流出而不是放出的试剂。然后，通过将注射器插入与用于抽吸或排出的孔不同的位置来吸取或排出试剂，进行稳定的抽吸或排出，从而获得稳定的测量值。版权所有（C）2011，JPO＆INPIT

8. 发明专利

JP2010200661A Simple and rapid method for extracting nucleic acid 审中-公开
标题翻译：用于提取核酸的简单和快速的方法
公开(公告)号：JP2010200661A
公开(公告)日：2010-09-16
申请号：JP2009048859
申请日：2009-03-03
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI , SOYA YOSHIHIRO , KYO MOTOKI
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for extracting a nucleic acid more simply and rapidly than a conventional method by targeting a nucleic acid-containing solution, especially a nucleic acid contained in an organism sample.
SOLUTION: Both liquid passage for sucking and discharging a nucleic acid-containing solution under atmospheric pressure and nucleic acid recovery efficiency are satisfied by using a monolith structure having a restricted liquid passage hole, diameter and thickness as a nucleic acid adsorption carrier of a nucleic acid recovery container.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种通过靶向含有核酸的溶液，特别是生物体样品中所含的核酸而比常规方法更简单和快速地提取核酸的方法。解决方案：通过使用具有限制的液体通过孔，直径和厚度的整料结构作为核酸吸附载体，可以满足在大气压下吸附和排出含核酸溶液的液体通道和核酸回收效率核酸回收容器。版权所有（C）2010，JPO＆INPIT

9. 发明专利

JP2008200006A Device for amplifying nucleic acid, container for amplifying the same and method for amplifying the same 有权
标题翻译：用于放大核酸的装置，用于放大核酸的容器和用于使其放大的方法
公开(公告)号：JP2008200006A
公开(公告)日：2008-09-04
申请号：JP2007042608
申请日：2007-02-22
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： KUSUMOTO MASAHIRO , TAKARADA YUTAKA , SOYA YOSHIHIRO
IPC分类号： C12M1/00 , C12N15/09 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a device for amplifying nucleic acid and a container for amplifying the same for performing a nucleic acid-amplifying reaction such as a PCR, etc., simply and in a short time.
SOLUTION: This device for amplifying the nucleic acid is equipped with a loading means for loading the container 10 for amplifying the nucleic acid and a first heating means 2 for forming a multiple number of heating zones adjusted at each of different temperatures on the loading means. Also, the container for amplifying the nucleic acid is provided by installing an opening part 11, a flow passage 12 and a vessel part 13 at a substrate plate 14, joining the opening part 11 with the vessel part 13 by the flow passage and having the opening part 11 as a cylindrical structure protruded from the substrate plate 14.
COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：简单且在短时间内提供用于扩增核酸的装置和用于扩增核酸的装置和用于进行核酸扩增反应如PCR等的容器。解决方案：用于扩增核酸的装置装备有用于装载用于扩增核酸的容器10的装载装置和用于形成多个加热区域的第一加热装置2，用于形成在每个不同温度下调节的多个加热区域装载手段。此外，用于扩增核酸的容器通过在基板14上安装开口部11，流路12和容器部13而设置，通过流路将开口部11与容器部13接合，并且具有开口部分11作为从基板14突出的圆柱形结构。版权所有（C）2008，JPO＆INPIT

10. 发明专利

JP2006333869A Method for simply detecting genetic polymorph and reagent for detecting the same 审中-公开
标题翻译：用于简单检测遗传多聚体和检测其的试剂的方法
公开(公告)号：JP2006333869A
公开(公告)日：2006-12-14
申请号：JP2006241628
申请日：2006-09-06
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , TAKARADA YUTAKA
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for clearly and reproducibly detecting genetic polymorph in several sites, especially the genetic polymorph in several sites of cytochrome 2D6, 2C19 and the like, and to provide a reagent therefor.
SOLUTION: The method for detecting the genetic polymorph comprises conducting amplification by using at least one of a wild type-detecting oligonucleotide and a variant-type detecting oligonucleotide and a nucleic acid-specific label, wherein a sequence having at least two variation sites different from each other is detected under an identical amplification condition.
COPYRIGHT: (C)2007,JPO&INPIT

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