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1. 发明专利

JP2008259453A Method for detecting nucleic acid 审中-公开
标题翻译：检测核酸的方法
公开(公告)号：JP2008259453A
公开(公告)日：2008-10-30
申请号：JP2007104938
申请日：2007-04-12
申请人： Institute Of Physical & Chemical Research , Toyobo Co Ltd , 東洋紡績株式会社 , 独立行政法人理化学研究所
发明人： ABE HIROSHI , ITO YOSHIHIRO , FURUKAWA KAZUHIRO , SHIUCHI KEIICHI , SOYA YOSHIHIRO
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78 , G01N33/53 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a nucleic acid quickly and accurately, and a kit for the same.
SOLUTION: This method for detecting the nucleic acid is provided by mixing a nucleic acid probe set containing a first nucleic acid probe and second nucleic acid probe each having a sequence complementary to that of the objective nucleic acid, with a sample containing the objective nucleic acid to develop a non-enzymatic bonding reaction, and the kit for the same is also provided.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供快速准确地检测核酸的方法及其用途。解决方案：用于检测核酸的方法通过将含有第一核酸探针的核酸探针组和各自具有与目的核酸的序列互补的序列的第二核酸探针与含有还提供了用于开发非酶结合反应的客观核酸，以及用于其的试剂盒。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2011155855A Internal control composition 有权
标题翻译：内部控制组合
公开(公告)号：JP2011155855A
公开(公告)日：2011-08-18
申请号：JP2010018120
申请日：2010-01-29
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide an internal control composition that does not reduce amplification performance of target gene and is widely useful regardless of kinds of target genes.
SOLUTION: The internal control composition is used for judging whether or not an amplification reaction is correctly performed in the amplification reaction of a nucleic acid of a target gene. The composition contains an internal control nucleic acid as a template and a primer pair for amplifying the internal control nucleic acid and the base sequences of the internal control nucleic acid and the primer pair are base sequences of a gene of an organism belonging to a region different from a region to which an organism from which the target gene is derived belongs.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供不降低靶基因的扩增性能的内部控制组合物，并且无论靶基因种类多广泛使用。解决方案：内部对照组合物用于判断在靶基因的核酸的扩增反应中是否正确进行扩增反应。该组合物含有作为模板的内部对照核酸和扩增内部对照核酸的引物对，并且内部对照核酸和引物对的碱基序列是属于不同区域的生物的基因的碱基序列来自靶基因所来自的生物体所属的区域。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2011110025A Method for dissolving acid fast bacteria 有权
标题翻译：解决酸性快速细菌的方法
公开(公告)号：JP2011110025A
公开(公告)日：2011-06-09
申请号：JP2009272061
申请日：2009-11-30
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method capable of efficiently dissolving acid fast bacteria such as tubercle bacillus safely at a low cost with simple operation.
SOLUTION: There is provided a method for dissolving acid fast bacteria, which dissolves the acid fast bacteria with a chaotropic salt solution of 40-90°C. Preferably, the concentration of the chaotropic salt in the solution is 2-6 M (mol/L), the pH of the chaotropic salt solution is 4.5-6.5, and the chaotropic salt solution further contains an acetate salt having a concentration of 0.1-2.0 M.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种能够以简单的操作以低成本安全地有效地溶解结核菌等酸性快速细菌的方法。解决方案：提供了一种溶解酸性快速细菌的方法，其用40-90℃的离液盐溶液溶解酸性快速细菌。溶液中离液盐的浓度优选为2-6M（mol / L），离液盐溶液的pH为4.5-6.5，离液盐溶液还含有浓度为0.1- 2.0 M.版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2010233505A Reagent kit for detecting nucleic acid amplification, having excellent preservation stability 有权
标题翻译：用于检测核酸放大的试剂盒，具有优异的保存稳定性
公开(公告)号：JP2010233505A
公开(公告)日：2010-10-21
申请号：JP2009085318
申请日：2009-03-31
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78
摘要： PROBLEM TO BE SOLVED: To provide a reagent for maintaining the performance of a reagent for detecting the nucleic acid amplification, usable for genetic screening in clinical diagnosis.
SOLUTION: The preservation stability is improved by constituting the reagent for detecting the nucleic acid amplification, usable for the reaction for detecting the nucleic acid amplification by PCR of two or more liquid compositions, and formulating (a) a reagent composition containing at least a fluorescent labeled probe, a magnesium ion and a buffer, and having a pH of ≥6.5 but
摘要翻译：待解决的问题：提供用于维持用于检测核酸扩增的试剂的性能的试剂，其可用于临床诊断中的遗传筛选。解决方案：通过构成用于检测核酸扩增的试剂，可用于通过两种或更多种液体组合物的PCR检测核酸扩增的反应，并配制（a）含有至少一种荧光标记的探针，一种镁离子和一种缓冲液，其pH值≥6.5但<8.5，和（b）在不同的液体组合物中含有至少一种DNA聚合酶和缓冲液的试剂组合物。版权所有（C）2011，JPO＆INPIT

5. 发明专利

JP2009039106A Oligonucleotide for species identification of acid-fast bacterium and use thereof 有权
标题翻译：用于鉴定酸性快速细菌的物种的寡核苷酸及其用途
公开(公告)号：JP2009039106A
公开(公告)日：2009-02-26
申请号：JP2008180128
申请日：2008-07-10
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： KUSUMOTO MASAHIRO , SHIUCHI KEIICHI
IPC分类号： C12N15/09 , C12Q1/04 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a rapid, reliable and simple method for the detection or the species identification of an acid-fast bacterium which is important on clinical diagnosis. SOLUTION: This method comprises carrying out a melting curve analysis with a probe whose target nucleic acid is a region having high bacteria species specificity in dnaJ1 gene in an acid-fast bacterium. The method comprises hybridizing a primer or an elongation product of the primer with an oligonucleotide probe used for detection and containing a nucleic acid sequence comprising at least ten continuous bases among specific nucleic acid sequences to form a complex, and then discriminating the formed complex. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：为临床诊断重要的酸快速细菌的检测或物种鉴定提供快速，可靠和简单的方法。解决方案：该方法包括使用目标核酸是在耐酸细菌中的dnaJ1基因中具有高细菌物种特异性的区域的探针进行解链曲线分析。该方法包括将引物或引物的延伸产物与用于检测的寡核苷酸探针杂交并含有在特定核酸序列中包含至少10个连续碱基的核酸序列以形成复合物，然后区分形成的复合物。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2008306935A Method for quickly detecting nucleic acid 有权
标题翻译：快速检测核酸的方法
公开(公告)号：JP2008306935A
公开(公告)日：2008-12-25
申请号：JP2007154873
申请日：2007-06-12
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI , KUSUMOTO MASAHIRO , KAMIKURA YOSHIKO
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/78 , G01N33/50 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting nucleic acid, by which the problem wherein the nucleic acid has not been detected quickly or with high sensitivity, due to inhibition of amplification in a nucleic acid detection method, using an intercalator and a labeling probe can be overcame, and by which the target nucleic acid can be detected quickly, and with high sensitivity. SOLUTION: The method for detecting the nucleic acid is characterized, by continuously or intermittently giving light having a wavelength for exciting an intercalator and measuring fluorescent light from a labeling probe in the presence of a sample for confirming the presence or quantity of the target nucleic acid and at least the intercalator and the labeling probe, and simultaneously applying a high speed PCR, to examine the presence or the quantity of the target nucleic acid. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种用于检测核酸的方法，其中由于核酸检测方法中的扩增的抑制，其中核酸没有快速或高灵敏度地检测到的问题，使用嵌入剂并且可以克服标记探针，并且可以快速地检测靶核酸，并且具有高灵敏度。解决方案：用于检测核酸的方法的特征在于，通过连续或间歇地给出具有用于激发嵌入剂的波长的光并在存在样品的情况下测量来自标记探针的荧光，以确认核酸的存在或数量目标核酸和至少嵌入剂和标记探针，并同时施加高速PCR，以检查靶核酸的存在或数量。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2011050401A Quick detection method of nucleic acid 审中-公开
标题翻译：核酸快速检测方法
公开(公告)号：JP2011050401A
公开(公告)日：2011-03-17
申请号：JP2010281262
申请日：2010-12-17
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI , KUSUMOTO MASAHIRO
IPC分类号： C12Q1/68 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To enable quick detection of a target nucleic acid while keeping specificity and high sensitivity by solving the problem of a conventional method using a nucleic acid probe or a nucleic acid primer whose fluorescent light quenches in hybridization with the target nucleic acid, and the problem that the nucleic acid probe interferes with a nucleic acid amplification process, the elongation process in particular, causing the sensitivity to drop when shortening the time and preventing the specific detection of the target nucleic acid by non-specific amplification of the nucleic acid primer.
SOLUTION: The relationship between the Tm value of a first primer and a second primer and Tm value of a marker probe and further the relationship between the concentration of the primer and the concentration of the marker probe are controlled and optimized to quickly detect the target nucleic acid while keeping specificity and high sensitivity.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了能够快速检测靶核酸，同时通过解决使用荧光灯与靶标杂交的核酸探针或核酸引物的常规方法的问题，同时保持特异性和高灵敏度以及核酸探针干扰核酸扩增过程，延伸过程特别是在缩短时间时引起灵敏度降低并通过非特异性扩增来防止靶核酸的特异性检测的问题核酸引物。解决方案：控制和优化第一引物和第二引物的Tm值与标记探针的Tm值之间的关系，并进一步确定引物浓度与标记探针的浓度之间的关系，以快速检测目标核酸同时保持特异性和高灵敏度。版权所有（C）2011，JPO＆INPIT

8. 发明专利

JP2010233503A Method for amplifying mycobacterium gene 有权
标题翻译：用于扩增MYCOBACTERIUM基因的方法
公开(公告)号：JP2010233503A
公开(公告)日：2010-10-21
申请号：JP2009085316
申请日：2009-03-31
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To solve such problems that operation of pretreatment is troublesome or much time is required for testing mycobacterium gene, and simplicity and rapidity are not satisfied.
SOLUTION: A method for amplifying the mycobacterium gene includes: a step of treating an untreated specimen containing the mycobacterium by an NALC-NaOH (N-acetyl-L-cysteine-sodium hydroxide) method; a step of suspending the sample treated in the step in water or a buffer, and providing a sample solution; and a step of mixing the sample solution obtained in the step with a nucleic acid amplifying reagent, and initiating the amplification.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：为了解决预处理操作麻烦或测试分枝杆菌基因需要大量时间的问题，并且不能满足简单性和快速性。解决方案：扩增分枝杆菌基因的方法包括：通过NALC-NaOH（N-乙酰基-L-半胱氨酸 - 氢氧化钠）方法处理含有分枝杆菌的未处理样品的步骤; 将在步骤中处理的样品悬浮在水或缓冲液中并提供样品溶液的步骤; 以及将在该步骤中获得的样品溶液与核酸扩增试剂混合并开始扩增的步骤。版权所有（C）2011，JPO＆INPIT

9. 发明专利

JP2010200660A Improved amplification reagent 有权
标题翻译：改进放大试剂
公开(公告)号：JP2010200660A
公开(公告)日：2010-09-16
申请号：JP2009048858
申请日：2009-03-03
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SOYA YOSHIHIRO , SHIUCHI KEIICHI , TAKARADA YUTAKA
IPC分类号： C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a new composition useful on the production of DNA from a template nucleic acid and an additional amplification of the DNA, and to provide a method therefor.
SOLUTION: The method for amplifying a nucleic acid for closing the upper portion of a reaction vessel comprising a polymer material with an air-permeable filter and contacting the nucleic acid in a sample to amplify the nucleic acid by a polymerase chain reaction is characterized in that an amplification reagent contains albumin and/or DMSO.
COPYRIGHT: (C)2010,JPO&INPIT

10. 发明专利

JP2007244375A Method for separation and purification of ribonucleic acid 审中-公开
标题翻译： RIBONUCLEIC ACID分离纯化方法
公开(公告)号：JP2007244375A
公开(公告)日：2007-09-27
申请号：JP2007023718
申请日：2007-02-02
申请人： Toyobo Co Ltd , 東洋紡績株式会社
发明人： SHIUCHI KEIICHI
IPC分类号： C12N15/09 , C12Q1/68 , G01N30/26
摘要： PROBLEM TO BE SOLVED: To provide a method for easily and safely separating and purifying a ribonucleic acid having high purity in high efficiency and short time and provide a reagent for the method.
SOLUTION: The invention provides a method for the separation and purification of a ribonucleic acid by contacting a solution containing the ribonucleic acid with an integrally formed porous material to adsorb the ribonucleic acid to the porous material, and a method for the separation and purification of a ribonucleic acid containing a step to separate the ribonucleic acid from the conjugated material obtained by the former method and composed of the ribonucleic acid and the porous material.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：解决的问题：提供一种在高效率和短时间内容易且安全地分离和纯化具有高纯度的核糖核酸并提供该方法试剂的方法。解决方案：本发明提供了通过将含有核糖核酸的溶液与一体形成的多孔材料接触以将核糖核酸吸附到多孔材料上来分离和纯化核糖核酸的方法，以及用于分离和纯化核糖核酸的方法，纯化含有通过前述方法获得的由核糖核酸和多孔材料组成的共轭材料的核糖核酸的步骤的核糖核酸。版权所有（C）2007，JPO＆INPIT

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