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1. 发明专利

JPH09281077A CAPILLARY ELECTROPHORETIC APPARATUS 失效
公开(公告)号：JPH09281077A
公开(公告)日：1997-10-31
申请号：JP9382796
申请日：1996-04-16
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , OKUYAMA NORIO , ITO YOSHITOSHI , TAKADA YASUAKI , OKUMURA AKIHIKO
IPC分类号： G01N27/447 , B01D57/02
摘要： PROBLEM TO BE SOLVED: To achieve the enhancement of capacity and miniaturization while expanding the applied field by adding either one of an electroosmotic flow pump and a flow rate regulating means controlling the flow of the liquid within a separation quartz capillary. SOLUTION: A liquid sending pump consists of a capillary element 10, a liquid tank 6 and the electrodes 11, 12 provided before and behind the capillary element 10 and the liquid sending amt. and flow velocity of the pump are optimized by selecting the kind, shape, size and applied voltage of the capillary element 10 and the kind, concn., pH and additive of a soln. The application of voltage to the pump becomes reverse to separation electrophoresis, that is, becomes a cathode 11 and an anode 12 but a separation cathode 9 and the pump cathode 11 are used in common to make it possible to form even a three- pole structure. The preparatory data from the auxiliary detector 20 provided to a separation capillary migration part other than detector 4 is fed back to an electrophretic condition controller 21 to be used in the control of separation or pump applied voltage.

2. 发明专利

JPH06213803A METHOD AND DEVICE FOR HIGH-SENSITIVITY DETECTION 失效
公开(公告)号：JPH06213803A
公开(公告)日：1994-08-05
申请号：JP814893
申请日：1993-01-21
申请人： HITACHI LTD
发明人： TAKI MAMORU , WATANABE YOSHIO , YOSHIDA MOTOKO , NAKAYAMA KANAE
IPC分类号： G01N21/01 , G01N21/27 , G01N21/59
摘要： PURPOSE:To perform replacement and positioning alignment of a capillary readily by aligning the position of a circular reflecting mirror once. CONSTITUTION:A cylindrical flow cell 5 is provided at the center of a cylindrical or spherical reflecting mirror 3 having an incident window 1 and an emitting window 2. The quartz flow cell 5, wherein a film layer around a part of a capillary 10 is removed, is provided in a through hole 11 provided at the center of the mirror with a screw 12 having the ferrule in the outer surface reflecting mirror 3 of the quartz sphere, wherein the incident window 1 and the emitting window 2 that are made to face approximately are provided. A photodetector 9 is arranged on the side of the emitting window 2. The focal point of incident light 6, which enters through the incident window 1, is focused in the vicinity of the center of the reflecting mirror 3. The light is transmitted through the flow cell. The reflection and the tansmittance are repeated many times in the facing reflecting mirror 3. Then, the transmitted light 7 is emitted from the emitting window 2 and cast into the photodetector 9.

3. 发明专利

JPH06118058A CAPILLARY GEL ELECTROPHORETIC DEVICE 失效
公开(公告)号：JPH06118058A
公开(公告)日：1994-04-28
申请号：JP26458692
申请日：1992-10-02
申请人： HITACHI LTD
发明人： NAKAYAMA KANAE , YOSHIDA MOTOKO , TAKI MAMORU , WATANABE YOSHIO
IPC分类号： B01D57/02 , G01N27/447
摘要： PURPOSE:To physically protect a gel on a capillary end easy to break with a porous film. CONSTITUTION:A gel charged capillary is formed of a fused quartz coated with a polyimide 1, a gel 3 for electrophoresis such as polyacrylamide gel is charged in the inner part, and a porous film 4 is mounted on the end part of the capillary. The porous film 4 is a film of an insulating material, which is formed of, for example, a material of polyester, polyamide, or polyethylene.

4. 发明专利

JPH0424658B2 失效
公开(公告)号：JPH0424658B2
公开(公告)日：1992-04-27
申请号：JP15208581
申请日：1981-09-28
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , ITO MICHIO
IPC分类号： G01N27/26 , G01N27/447

5. 发明专利

JPH0392755A DETECTION OF DIFFERENCE BETWEEN TWO-DIMENSIONAL ELECTROPHORETIC IMAGES 失效
公开(公告)号：JPH0392755A
公开(公告)日：1991-04-17
申请号：JP22915289
申请日：1989-09-06
申请人： HITACHI LTD
发明人： ITO MICHIO , YOSHIDA MOTOKO
IPC分类号： G01N27/447
摘要： PURPOSE:To detect the minute difference between protein mixtures sensitively by obtaining the product of the three values, i.e. the sum of absorbances, the changing rate of the area and the deviation of the central position of the chromatin spot of the protein component wherein two two-dimensional electrophoresis images obtained under the same migrating conditions are segregated. CONSTITUTION:Rhodamine is injected into a rat, and a rhodamine sarcoma is developed. The sarcoma is transplanted into the back part of another DONRYU rat. After the transplant, the serum protein is analyzed by an electrphoresis method with the elapse of time. An electrophoresis image wherein the spot of the segregated protein clearly appears is formed. The image data are inputted into a computer through a TV camera, and the image is analyzed. Namely, the sum of the absorbances, the change in area and the deviation of the central position of the chromatin spot are obtained for every segregated spot, and the product of the value is computed. Thus, the change in serum protein due to the sarcoma is detected with good sensitivity.

6. 发明专利

JPS61104247A Two-dimensional electrophoretic apparatus 失效
标题翻译：二维电泳装置
公开(公告)号：JPS61104247A
公开(公告)日：1986-05-22
申请号：JP22574084
申请日：1984-10-29
申请人： Hitachi Ltd
发明人： YOSHIDA MOTOKO , OKANO KAZUNOBU , ITO MICHIO , KANETOMO MASABUMI
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44704
摘要： PURPOSE:To effectively control the temp. of agarose, by directly contacting a sheet like heater with a container for receiving agarose. CONSTITUTION:A definite amount of agarose conditioned and melted is preliminarily sucked in a syringe container 3 and the whole of the container containing a syringe needle 4 is enveloped by a sheet like heater 6 and controlled to proper temp. through an insulating transformer 9 and a slide resistor 10. In this case, operation for moving a shaft 11 by a syringe driving motor 15 in such a state that a syringe is attached to a moving stand 7 and a structure for moving said shaft 11 by a syringe linearily moving drive motor 8 are employed.
摘要翻译：目的：有效控制温度。的琼脂糖，通过将片状加热器直接接触用于接收琼脂糖的容器。构成：将预定量的琼脂糖调节和熔化预先吸入注射器容器3中，并且包含注射器针4的整个容器被片状加热器6包围并被控制到适当的温度。通过绝缘变压器9和滑动电阻器10.在这种情况下，通过注射器驱动电动机15在注射器附接到移动支架7的状态下移动轴11的操作和用于使所述轴11移动的结构通过采用线性移动驱动马达8的注射器。

7. 发明专利

JPS60106995A ELECTROPHORESIS DEVICE 失效
公开(公告)号：JPS60106995A
公开(公告)日：1985-06-12
申请号：JP21396783
申请日：1983-11-16
申请人： HITACHI LTD
发明人： KANETOMO MASABUMI , MATSUMURA YASUHIDE , YOSHIDA MOTOKO
IPC分类号： C25D13/00
摘要： PURPOSE:To achieve automation of a substrate treating stage and a dyeing stage by preparing a treating tank, treating liquid tank and waste liquid tanks and connecting these tanks via respective solenoid valves by means of pipings and changing over the solenoid valves to conduct the treating liquid to the treating tank. CONSTITUTION:Substrates 2 are disposed in a treating tank 1 and four solenoid valves 3 and the same number of treating liquid tanks 4 are provided above the same and are connected respectively by a piping 5. Four solenoid valves 6 and waste liquid tanks 7 are provided below the tank 1 and are connected to each other similarly by a piping 10. These valves 3, 6 are controlled by a controller 8. The treating liquid 9 contained in the tanks 4 is fed to the tank 1 by opening and closing of the valves 3 and the substrates 2 are treated. The valves 6 are opened upon ending of the treatment to conduct the liquid 9 to the prescribed tanks 7. The similar operation is repeatedly performed with the other treating liquid as well.

8. 发明专利

JPS58144737A HIGH-SPEED DYEING METHOD FOR SEPARATE PROTEIN 失效
公开(公告)号：JPS58144737A
公开(公告)日：1983-08-29
申请号：JP2743082
申请日：1982-02-24
申请人： HITACHI LTD
发明人： ITOU MICHIO , YOSHIDA MOTOKO , SAKAIRI MINORU
IPC分类号： G01N27/26 , C12Q1/28 , G01N27/447 , G01N33/68
摘要： PURPOSE:To perform a high-sensitive and a high-speed dyeing of protein, by a method wherein, when protein migrates and separates, a hemin, simultaneously, is moved in a support by an electrophoresis to adsorb it to protein. CONSTITUTION:Firstly, a blood protein is separated in polyacrylamide gel by an electrophoresis method. Then, when the protein migrates and separates, a hemin, simultaneously, is brought to migrate in the gel to adsorb it to the protein. The protein is then converted into a material having a peloxydase action, and the protein is dyed by the action for detection. This enables to perform a high-sensitive and high-speed dyeing of a protein in a polyacrylamide gel and an efficient diagnosing operation of sickness based on a blood protein analysis.

9. 发明专利

JPS58105053A METHOD OF TWO-DIMENTIONAL ELECTROPHORESIS 失效
公开(公告)号：JPS58105053A
公开(公告)日：1983-06-22
申请号：JP20373581
申请日：1981-12-18
申请人： HITACHI LTD
发明人： ITOU MICHIO , YOSHIDA MOTOKO
IPC分类号： G01N27/26 , G01N27/447
摘要： PURPOSE:To improve a separating power sharply by using a plane-shaped gel having the concentration gradient of polyacrylamide and containing a buffer liquid having alkaline hydrogen ion concentration from an isoelectric point of a component to be inspected. CONSTITUTION:One surface of a glass plate 1 is processed by methacryl oxypropyltrimethoxysilane, and thereby a plane gel 2 having the concentration gradient of 4-20% and containing a buffer liquid (PH-8.6, ion strength-0.06, A liquid) composed of 5,5-diethylbarbituric acid sodium and 5,5-diethylbarbituric acid is prepared. A serum is given into a cut 3 made in the low-concentration part of the gel. Filter paper sheets 4 and 5 for liquid junction, which are impregnated with the A liquid, are connected to a negative electrode tank 6 and a positive electrode tank 7 with the A liquid put therein, and serum protein is separated by electrophoresis. The liuid junction being removed, filter paper sheets 8 and 9 for liquid junction, which are impregnated with the A liquid, are connected to a negative electrode tank 10 and a positive electrode tank 11 with the A liquid put therein, and the serum protein is separated according to the difference in molecular weight.

10. 发明专利

JPS5539209A TWOODIMENTIONAL CATAPHORESIS METHOD 失效
公开(公告)号：JPS5539209A
公开(公告)日：1980-03-19
申请号：JP11077178
申请日：1978-09-11
申请人： HITACHI LTD
发明人： YOSHIDA MOTOKO , ITOU MICHIO
IPC分类号： B01D57/02 , G01N27/26 , G01N27/447
摘要： PURPOSE:To carry out two-dimentional cataphoresis using the same plate gel and to improve analytical method of blood serum protein etc., by giving the concentration gradient of polymer gel in one direction of the plate and using the plate gel added amphoteric electrolyte to the lower concentration part. CONSTITUTION:Polymer gel, such as acrylamide etc., having molecular sieve effect, is formed on the plate put in the vessel at about 2.5-40% of concentration gradient. About 0.5-2wt% of amphoteric electrolyte composed of reaction product etc. of amine, such as triethyleneteramine etc., having secondary amino radical and alpha, beta unsaturated acid, such as acrylic acid etc., is added to diluted side of the concentration gradient. Electrodes are provided at both ends of the plate and the sample to be tested is covered on the diluted part of polymer gel concentration and then, the one-dimentional cataphoresis is carried out in a constant direction of the polymer gel. Next, two-dimentional cataphoresis is carried out in the concentration gradient direction of the polymer gel. By the above method, automation of the apparatus is able to carry out.

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