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1. 发明专利

JP2006006274A Gene testing method 有权
标题翻译：基因测试方法
公开(公告)号：JP2006006274A
公开(公告)日：2006-01-12
申请号：JP2004191781
申请日：2004-06-29
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： NAGAI KEIICHI , OKANO KAZUNOBU , NODA HIDEYUKI , MATSUNAGA HIROKO , TANIGUCHI KIYOMI , YAZAWA YOSHIAKI , KAJIYAMA TOMOHARU
IPC分类号： C12Q1/68 , C12N15/09 , G01N21/76 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/6827 , C12Q1/6837 , C12Q2565/537 , C12Q2565/301 , C12Q2525/155
摘要： PROBLEM TO BE SOLVED: To provide a gene testing method capable of testing single nucleotide polymorphisms (SNPs) in a plurality of variation regions at a low cost by a simple operation and capable of realizing gene diagnosis on a clinical site. SOLUTION: This gene testing method comprises a process for hybridizing a sample nucleic acid having an anchor sequence at the 5' end with a support having a surface to which a probe containing a sequence complementary to a sequence (SNP region) to be detected is immobilized, a process for conducting elongation reaction of a complementary chain of the probe by using the sample nucleic acid as a template, a process for dissociating and removing the sample nucleic acid from the elongated chain of the probe synthesized in the elongation reaction, a process for conducting elongation reaction of a complementary chain with a primer having a sequence identical to the anchor sequence by using the elongated chain of the probe dissociated in the above as a template, and a process for detecting pyrophosphoric acid formed by the elongation reaction of the primer with biochemical luminescence, so that an SNP type of the sample nucleic acid is determined. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供能够通过简单的操作以低成本测试多个变异区域中的单核苷酸多态性（SNP）的基因测试方法，并且能够在临床现场实现基因诊断。解决方案：该基因测试方法包括将具有5'端锚定序列的样品核酸与具有表面的载体杂交的方法，所述载体具有包含与序列互补序列（SNP区）的探针的表面检测到的固定化物，通过使用样品核酸作为模板进行探针的互补链的伸长反应的方法，从延伸反应合成的探针的细长链中解离和除去样品核酸的方法，通过使用在上述中解离的探针的细长链作为模板，使互补链与具有与锚定序列相同的序列的引物进行延伸反应的方法，以及通过延伸反应形成的焦磷酸的检测方法该引物具有生化发光，从而确定样品核酸的SNP型。版权所有（C）2006，JPO＆NCIPI

2. 发明专利

JP2005241520A Excreta filtration device and excreta filtration method 审中-公开
标题翻译： EXCRETA过滤装置和EXCRETA过滤方法
公开(公告)号：JP2005241520A
公开(公告)日：2005-09-08
申请号：JP2004053615
申请日：2004-02-27
申请人： Hitachi Ltd , National Cancer Center-Japan , 国立がんセンター総長 , 株式会社日立製作所
发明人： MATSUMURA YASUHIRO , MATSUSHITA NAOYUKI , NOGUCHI KIYOTERU , OKANO KAZUNOBU , HARADA KUNIO , TSUNODA HIROYUKI , NAGAI KEIICHI , MITSUYAMA SATOSHI
IPC分类号： G01N33/48 , B04B3/00 , B04B7/00 , B04B7/08 , G01N1/10 , G01N1/28 , G01N33/574
摘要： PROBLEM TO BE SOLVED: To provide an excreta filtration device and an excreta filtration method used for retrieving cells from excreta in order to examine colon cancer from the ordinary excreta of multispecimen.
SOLUTION: This excreta filtration device has a circular cone-shaped or cylindrical filter 1 for filtering a mixture 7 of the ordinary excreta and buffer liquid, a support mechanism 2 having a porous or mesh structure where the filter 1 is mounted, a mechanism 5, 6 for rotating the filter 1 and the support mechanism 2, and a container 3 for sampling filtrate filtered by a centrifugal force provided on the peripheral part of the support mechanism 2.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供排泄物过滤装置和用于从排泄物中回收细胞的排泄物过滤方法，以便从多通道的普通排泄物检查结肠癌。解决方案：该排泄物过滤装置具有用于过滤普通排泄物和缓冲液的混合物7的圆锥形或圆柱形过滤器1，具有安装过滤器1的多孔或网状结构的支撑机构2，用于旋转过滤器1和支撑机构2的机构5,6以及用于对通过设置在支撑机构2的周边部分上的离心力过滤的滤液进行取样的容器3。版权所有（C）2005，JPO＆NCIPI

3. 发明专利

JP2004283083A On-line chemical reaction unit and analysis system therefor 审中-公开
公开(公告)号：JP2004283083A
公开(公告)日：2004-10-14
申请号：JP2003079346
申请日：2003-03-24
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： HIRABAYASHI TSUDOI , OBARA MASANOBU , OKANO KAZUNOBU
IPC分类号： G01N27/62 , B01F11/00 , B01F13/00 , B01J19/00 , B01J19/24 , C12M1/34 , C12P1/00 , C12P21/06 , C12Q1/37 , G01N30/06 , G01N30/46 , G01N30/72 , G01N30/86 , G01N30/88 , G01N31/20 , G01N35/10
CPC分类号： G01N35/1097 , B01F11/0071 , B01F13/0059 , B01J19/0093 , B01J2219/00788 , B01J2219/00831 , B01J2219/00835 , B01J2219/0086 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/0095 , B01J2219/00984
摘要： PROBLEM TO BE SOLVED: To provide a chemical reaction method and a chemical reaction unit capable of making a chemical reaction treatment of a minute amount of a biological sample with low loss and also capable of increasing the collision frequency between molecules undergoing chemical reaction for seeking sample loss reduction and treating the sample in a short time, in order to solve the problems that in a batch-based enzyme reaction, sample loss is inevitable, and long reaction time has been inevitable in the conventional techniques of seeking sample loss reduction. SOLUTION: The chemical reaction unit works as follows: A tubular reactor 1 is immobilized with a chemical substance and packed with a buffer solution. A predetermined volume of air is sucked via an air inlet port 3 and a sample liquid is then sucked, and furthermore a predetermined volume of air is sucked. Air, the sample liquid and the air are transported to the reactor 1 successively. Thus, the reactor 1 is filled with the sample liquid, both ends of the sample liquid are sandwiched in between the air for preventing the sample liquid from mixing with the buffer solution. The sample liquid undergoes reciprocating motion at a predetermined flow, thus promoting a highly efficient chemical reaction. COPYRIGHT: (C)2005,JPO&NCIPI

4. 发明专利

JP2003315336A Particle array manufacturing method and its device 有权
标题翻译：颗粒阵列制造方法及其装置
公开(公告)号：JP2003315336A
公开(公告)日：2003-11-06
申请号：JP2002117487
申请日：2002-04-19
申请人： Hitachi Ltd , 株式会社日立製作所
发明人： NODA HIDEYUKI , OBARA MASANOBU , OKANO KAZUNOBU
IPC分类号： G01N33/53 , B01J19/00 , B01L3/00 , B01L3/02 , C12N15/09 , G01N15/00 , G01N35/00 , G01N35/02 , G01N37/00
CPC分类号： B01L3/021 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00353 , B01J2219/00414 , B01J2219/00463 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00585 , B01J2219/00599 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00689 , B01J2219/00704 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/502761 , B01L2200/0657 , B01L2200/0668 , B01L2300/0838 , B01L2400/0487 , B01L2400/049 , G01N35/028 , G01N2035/00574 , Y10T436/119163 , Y10T436/2575
摘要： PROBLEM TO BE SOLVED: To provide a new method and a technology for arraying particles as desired. SOLUTION: (1) A particle capturing capillary having a smaller inner diameter than outer diameters of probe immobilization particles to be used is prepared. (2) Only one particle is taken out by adsorption on an opening part of the tip from inside a storage part where a plurality of particles are held by suction from inside the particle capturing capillary. (3) The particle adsorbed on the opening part of the tip of the particle capturing capillary is positioned on an opening part of a particle arraying capillary for arraying the particles or on the passage end of a chip where a passage having a gateway having the little larger width than the outer diameter of the particle so that only one particle can pass is provided. (4) The particle adsorbed on the opening part of the tip of the particle capturing capillary is injected into the particle arraying capillary or the passage end of the chip from the opening part of the particle arraying capillary. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供根据需要排列颗粒的新方法和技术。解决方案：（1）制备具有比要使用的探针固定颗粒的外径小的内径的颗粒捕获毛细管。（2）通过从捕集毛细管内部抽吸多个粒子的收纳部的内部，从尖端的开口部吸附而仅吸附一个粒子。（3）吸附在捕集毛细管尖端的开口部分的颗粒位于颗粒排列毛细管的开口部分上，用于排列颗粒或芯片的通道端，其中通道具有少量的通道比颗粒的外径大的宽度，从而仅提供一个颗粒可以通过。（4）吸附在捕集毛细管尖端的开口部的粒子从粒子排列毛细管的开口部注入粒子排列毛细管或芯片的通路端。版权所有（C）2004，JPO

5. 发明专利

JP2000088804A FRACTIONATING DEVICE 失效
公开(公告)号：JP2000088804A
公开(公告)日：2000-03-31
申请号：JP25484698
申请日：1998-09-09
申请人： HITACHI LTD
发明人： MATSUNAGA HIROKO , UEMATSU SENSHU , OKANO KAZUNOBU
IPC分类号： G01N27/447
摘要： PROBLEM TO BE SOLVED: To cut an objective portion out from a gel to fractionate it. SOLUTION: An agarose gel containing a sample developed by electrophoresis is set on an X-Y stage 2 using gel stoppers 3, 4. The sample labeled with a phosphor is detected by a light source 6 and a two-dimensional camera 7 to display an electrophoresis pattern on a monitor 8 as an image data. A cutting- out portion is selected in reference to the image data, and the selected portion is automatically cut from the gel using cutting-out parts 9, 10. An oblective portion is accurately cut out thereby to be fractionated.

6. 发明专利

JP2000037193A PREPARATION OF NUCLEIC ACID SAMPLE FOR MICROGENE ANALYSIS, PREPARED NUCLEIC ACID SPECIMEN, ANALYSIS OF NUCLEIC ACID SPECIMEN USING THE SAME, REAGENT KIT THEREFORE AND ANALYSIS SERVICE 失效
公开(公告)号：JP2000037193A
公开(公告)日：2000-02-08
申请号：JP13805199
申请日：1999-05-19
申请人： HITACHI LTD
发明人： MURAMATSU TAKAMICHI , FUJITA TAKESHI , KIYAMA MASAHARU , IRIE TAKASHI , OKANO KAZUNOBU
IPC分类号： C12N15/09 , C12Q1/68 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To obtain a nucleic acid specimen for analysis of the expression of microgene by receiving an unreacted product after a nucleic acid specimen containing plural kinds of sequences for analysis is mixed and combined with a probe carrier specifically combining with a part of the sequences. SOLUTION: This is the method for obtaining a nucleic acid specimen for microgene analysis capable of carrying out the analysis of the expression of microgene in a high resolution. The specimen obtained by previously preparing a nucleic acid sample having plural kinds of sequences for analysis and one or plural kinds of probe carriers having the known sequence specifically combining with a part of sequence in the above nucleic acid sample, mixing the above nucleic acid specimen with the above probe carrier to combine, then treating the sequence combined with the above probe carrier by the use of a degradation enzyme or the probe carrier itself having enzymatic activity and recovering the above nucleic acid specimen not combined with the above probe carrier or the nucleic acid specimen synthesized on the basis of the above nucleic acid specimen.

7. 发明专利

JPH11304666A SAMPLE HANDLING TOOL AND METHOD FOR USING SAME 失效
公开(公告)号：JPH11304666A
公开(公告)日：1999-11-05
申请号：JP11461498
申请日：1998-04-24
申请人： HITACHI LTD
发明人： MURAKAWA KATSUJI , OKANO KAZUNOBU
IPC分类号： G01N33/48 , C12M1/00 , G01N1/00 , G01N1/10 , G01N27/26
摘要： PROBLEM TO BE SOLVED: To enable the simultaneous and simple operations of determination, isolation, transferring, holding, and mixing of a large number of trace amounts of liquid samples or microorganism samples by arranging micro hydrophilic regions on a plane and a hydrophobic region around it and using the hydrophilicity of samples to the hydrophilic regions. SOLUTION: A matrix of hydrophilic regions 2 is provided on a plane, and a hydrophobic region 3 is provided around them. A liquid 4 on a handling tool 1 for liquid samples is repelled on the hydrophobia region 3 and is stably held only on the hydrophilic regions 2. The size of the hydrophilic region 2 is normally 1 mm or less in diameter. Its shape may be circular, rectangular or in other shapes. The number of the hydrophilic regions 2 per handling tool for liquid samples is not especially limited. However, it is preferably 96 pieces or more for multi-sample processing, and it is preferably a multiple of 4 in the case of DNA samples. The base material of the tool 1 is not especially limited. However, a heat resisting material such as heat resisting plastic, glass, silicon, metal is suitable for the purpose of performing a reaction using heat resisting enzymes at temperatures close to 100 deg.C.

8. 发明专利

JPH11164700A DETECTION OF DNA 失效
公开(公告)号：JPH11164700A
公开(公告)日：1999-06-22
申请号：JP33468297
申请日：1997-12-04
申请人： HITACHI LTD
发明人： KANBARA HIDEKI , OKANO KAZUNOBU
IPC分类号： G01N33/483 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/52 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting DNA by simultaneously irradiating an intercalator, which enters a double-stranded DNA and emits fluorescence by light irradiation, and a fluorescent substance bound to the DNA, and assaying the quantity and length of the DNA through measurement of fluorescence intensity of both the fluorescent probes. SOLUTION: In this method, a fluorescence-labeled DNA probe in added to a solution containing a specimen, and by hybridizing the probe with a target DNA to produce a double-stranded DNA, adding an intercalator (e.g. ethidium bromide), which enters a double-stranded DNA and emits fluorescence by light irradiation to the double-stranded DNA, simultaneously irradiating the intercalator and the fluorescent substance of the fluorescence-labeled DNA probe bound to the DNA contained in the solution, measuring the intensity of fluorescence emitted by the above intercalator and the intensity of fluorescence emitted by the above fluorescent substance, and assaying a signal dependent on the number of the DNA molecules and information on the chain length of the DNA, based on the emission intensity for the labeling fluorescent substance and the emission intensity ratio for both the fluorescent probes, the DNA for DNA diagnosis, analysis and the like, is detected.

9. 发明专利

JPH08332100A PRIMER FOR DNA POLYMERASE REACTION AND DETERMINATION OF POLYNUCLEOTIDE SEQUENCE USING THE SAME 失效
公开(公告)号：JPH08332100A
公开(公告)日：1996-12-17
申请号：JP13905195
申请日：1995-06-06
申请人： HITACHI LTD
发明人： OKANO KAZUNOBU , KANBARA HIDEKI
IPC分类号： C12N15/09 , C07H21/04 , C12Q1/68
摘要： PURPOSE: To obtain an primer by application of a new DNA polymerase, having a structure containing a polynucleotide comprising an optional simple base and a complementary sequence in a cleaved part with a restriction enzyme at the 3'-terminal and capable of determining the base sequence of a DNA according to simple operations. CONSTITUTION: This new primer 6 for polymerase reaction comprises a structure containing a polynucleotide 7 comprising an optional simple base and a sequence 8 complementary to a cleaved part with a restriction enzyme at the 3'-terminal of the polynucleotide 7. A sample DNA is cleaved with the specific restriction enzyme and a simple base is polymerized and introduced into the 3'-terminal of the sample DNA by using a terminal deoxynucleotidyltransferase. The resultant DNA is then hybridized with the primer and the DNA polymerase reaction is carried out to successively form a base sequence part 9 following a recognition part 8 with the restriction enzyme of the primer 6. Thereby, the polynucleotide sequence of the sample DNA is determined-according to simple operations.

10. 发明专利

JPH06174693A DIVIDING AND FRACTIONATING DEVICE 失效
公开(公告)号：JPH06174693A
公开(公告)日：1994-06-24
申请号：JP32199192
申请日：1992-12-01
申请人： HITACHI LTD
发明人： KANBARA HIDEKI , OKANO KAZUNOBU , TAKAHASHI SATOSHI
IPC分类号： G01N27/447 , B01D59/42 , G01N30/80
摘要： PURPOSE:To prevent a specimen from loosing due to the adsorption and from lowering the efficiency of division by locating a specimen dividing cell array in the way of an electrophoretic migration passage or a specimen passage right after the migration passage. CONSTITUTION:A DNA piece specimen is injected in an upper portion 11 of a capillary gel. A fluorescent indication is applied to the specimen and a voltage is applied to electrodes 9, 10 to cause the electrophoretic migration thereof. The specimen is divide in accordance with the size and the appearance can be monitored by means of a fluorescent detector 14. The specimen passes through a small hole 13 to enter dividing cells 3. The specimen stops at the region for a long time. When the passing of the specimen is detected by the fluorescent monitoring, a dividing cell cartridge 2 is moved by means of a motor 15 from the detecting section to the dividing section by delaying a time wherein an electrophoretic migration time is considered. Each of the cells 3 are supported between upper and lower support plates 4, 5 so that the specimen is held therein. Each of the cells 3 has small hole 12 opened on the lower respective section and the support plate 5 has small holes corresponding to the cells to form a respective electrophoretic passage. Each small hole on the bottom of each of the cells 3 is so sized as to neglect a leakage of DNA therethrough.

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