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    • 1. 发明专利
    • Biological electron microscope and method of sample observation
    • 生物电子显微镜和样品观察方法
    • JP2004212355A
    • 2004-07-29
    • JP2003002724
    • 2003-01-09
    • Hitachi High-Technologies CorpHitachi Ltd株式会社日立ハイテクノロジーズ株式会社日立製作所
    • KAKIBAYASHI HIROSHIHOSOKI SHIGEYUKITAKAGI YUJIMIYAKE AKIRANAKAMURA KUNIYASUSATO MITSUGIKOBAYASHI HIROYUKI
    • G01N23/04G01N1/28G01N23/225G01N33/483G01N37/00H01J37/20H01J37/22H01J37/26H01J37/28
    • H01J37/22H01J37/26H01J2237/05H01J2237/2809H01J2237/2813
    • PROBLEM TO BE SOLVED: To implement a biological electron microscope and an observation method capable of observing a biosample with high contrast, without significant damages, and of analyzing images with high precision, and performing high-throughput sample pretreatments. SOLUTION: (1) Observation is performed by an accelerating voltage 1.2 to 4.2 times the sample transmission critical accelerating voltage of electron beams which is determined under prescribed conditions. (2) An E×B type energy filter of a compact and simple structure is provided between the sample and an electron beam detector, and an image is formed by electron beams existing within a specific energy region among electron beams transmitted through the sample. (3) Quantitative analysis is carried out by image processings on similarities among observed images of viruses, proteins, etc. in the sample and reference images of known viruses, proteins, etc. (4) A biosample pretreatment protocol is formed into a chip through the use of the MEMS technology, and the chip is mounted on a sample stage part of the electron microscope and will carry out introduction, pretreatment, and until the transfer of the sample to a sample holder. COPYRIGHT: (C)2004,JPO&NCIPI
    • 要解决的问题:实现能够以高对比度观察生物样品,没有显着损害和高精度分析图像并进行高通量样品预处理的生物电子显微镜和观察方法。 解决方案:(1)通过在规定条件下确定的电子束的样品传输临界加速电压的1.2〜4.2倍的加速电压进行观察。 (2)在样品和电子束检测器之间提供了紧凑且简单的结构的E×B型能量过滤器,并且通过在穿过样品的电子束中存在于特定能量区域内的电子束形成图像。 (3)通过图像处理对样品中的病毒,蛋白质等的观察图像和已知病毒,蛋白质等的参考图像之间的相似性进行定量分析。(4)生物样品预处理方案形成为芯片通过 使用MEMS技术,将芯片安装在电子显微镜的样品台部分,并进行引入,预处理,直到样品转移到样品架上。 版权所有(C)2004,JPO&NCIPI
    • 5. 发明专利
    • ELECTRON MICROSCOPE DEVICE
    • JPH08106873A
    • 1996-04-23
    • JP23978494
    • 1994-10-04
    • HITACHI LTD
    • KAKIBAYASHI HIROSHINAKAMURA KUNIYASUTOKIDA RURIKO
    • H01J37/20H01J37/22
    • PURPOSE: To precisely and automatically correct a slippage by providing a sample rotating mechanism, a sample moving mechanism, and their control means, and receiving a generated position correcting signal to return an area to be observed to the space position before the inclination of a sample. CONSTITUTION: The inclination of an actual sample 14 is kept in 0 deg., a sample moving mechanism 5 is driven by a CPU 11 while observing its electron microscopic image on a display to move a specified area to be observed to the center of the visual field. The sample, 14 is then inclined by a required angle by a sample rotating mechanism 6, the slippage of the required angle is determined on the basis of the preliminarily stored slippage information by the CPU, and a position correcting signal of the value of the reversed sign is generated and transmitted to a sample moving mechanism control system 8. The control system 8 moves the position of the actual sample 14 so that the area to be observed after inclination is returned to the space position before inclination. Thus, the crystal structure of the sample can be regularly observed with a high magnification and the same visual field normal focus.
    • 8. 发明专利
    • SCANNING ELECTRON MICROSCOPE
    • JP2000188077A
    • 2000-07-04
    • JP36405898
    • 1998-12-22
    • HITACHI LTD
    • NAKAMURA KUNIYASUKAKIBAYASHI HIROSHIICHIHASHI MIKIOISAGOZAWA SHIGETOSATO YUJIHASHIMOTO TAKAHITO
    • H01J37/09H01J37/28H01J37/295
    • PROBLEM TO BE SOLVED: To observe defects other than a sample matrix on a scanning transmission image or impurities at high contrast by two-dimensionally scanning a fine probe formed with a convergent lens and an objective lens by accelerating an electron beam with an electrostatic lens, and masking specified electron beams with a moving diaphragm installed on a detector for detecting the intensity of scattered electron beams. SOLUTION: Electron beams are reduced through first to third step lenses 2a-2c for accelerating to a predetermined voltage, first and second convergent lenses 3a, 3b, and an objective front magnetic field lens 4, and a probe on a sample 5 changes an aperture angle with a convergent diaphragm 6. An electron beam diffraction pattern formed after transmitting the sample 5 changes magnification with first and second step projection lenses 8a, 8b, projects to an electron beam detector 9, and is modulated in brightness. First and second step detector diaphragms 12a, 12b in the lower part of the second step projection lens 8b move in about four steps, have many kinds of mask patterns such as an electron beam diffraction pattern or round holes, mask a substrate crystal relating part, and enhance contrast in other part.
    • 9. 发明专利
    • Observation apparatus and method using electron beam
    • 观察装置和使用电子束的方法
    • JP2006153894A
    • 2006-06-15
    • JP2006058855
    • 2006-03-06
    • Hitachi Ltd株式会社日立製作所
    • TAKAGUCHI MASANARINAKAMURA KUNIYASUUMEMURA KAORUTANIGUCHI YOSHIFUMIICHIHASHI MIKIO
    • G01N23/20H01J37/28
    • PROBLEM TO BE SOLVED: To provide an apparatus, a technique, and a method for two-dimensional distributions and measuring distortions and stress, having nanometer resolution of each type of crystal sample through the use of diffraction images observed by an electronic microscope.
      SOLUTION: Electron beams are irradiated in minute amount and in parallel to a sample. The distances, between the spots of a diffraction image reflecting a crystal structure acquired by the irradiation, are measured by an image detector or a position detector. A two-dimensional stress distribution is superimposed on the measured positional information and displayed on an enlarged image of an electronic microscope. Distortions and stresses in minute crystals can be displayed at a high resolution and at a high speed in matching structural information of the sample.
      COPYRIGHT: (C)2006,JPO&NCIPI
    • 要解决的问题:提供一种用于二维分布和测量失真和应力的装置,技术和方法,通过使用电子显微镜观察到的衍射图像,具有每种类型的晶体样品的纳米分辨率 。 解决方案:电子束以微量和平行于样品照射。 通过图像检测器或位置检测器测量反射通过照射获得的晶体结构的衍射图像的光点之间的距离。 将二维应力分布叠加在所测量的位置信息上并显示在电子显微镜的放大图像上。 微分晶体中的变形和应力可以以高分辨率和高速显示,以匹配样品的结构信息。 版权所有(C)2006,JPO&NCIPI