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    • 2. 发明专利
    • Protein having cellulase activity and utilization thereof
    • 具有纤维素酶活性的蛋白质和其利用
    • JP2012039968A
    • 2012-03-01
    • JP2010185616
    • 2010-08-20
    • Toyota Central R&D Labs Inc株式会社豊田中央研究所
    • IKEUCHI AKINORIIMAMURA CHIEITO YOICHIROTAKAHASHI HARUO
    • C12N15/09C12N1/15C12N1/19C12N9/42C12P19/14
    • Y02P20/52
    • PROBLEM TO BE SOLVED: To provide a cellulase having improved activity.SOLUTION: The protein having the cellulase activity comprises the following (a) to (d): (a) an amino acid sequence having a specific sequence; (b) an amino acid sequence in which the amino acid sequence having the specific sequence contains one or several amino acid mutations of substitution, deletion, insertion and/or addition, and which has the cellulose-binding activity; (c) an amino acid sequence which has ≥85% identity with the amino acid sequence having the specific sequence, and which has the cellulose-binding activity; and (d) an amino acid sequence which is encoded by a DNA hybridizing with the base sequence encoding the amino acid sequence comprising the specific sequence or a DNA complementary to a part thereof under a stringent condition and has the cellulose-binding activity.
    • 要解决的问题:提供具有改善的活性的纤维素酶。 解决方案:具有纤维素酶活性的蛋白质包括以下(a)至(d):(a)具有特定序列的氨基酸序列; (b)具有特定序列的氨基酸序列含有一个或几个氨基酸的取代,缺失,插入和/或添加突变,并具有纤维素结合活性的氨基酸序列; (c)与具有特定序列的氨基酸序列具有≥85%同一性且具有纤维素结合活性的氨基酸序列; 和(d)由在严格条件下与编码包含特定序列的氨基酸序列的碱基序列的碱基序列或与其部分互补的DNA杂交并具有纤维素结合活性的DNA编码的氨基酸序列。 版权所有(C)2012,JPO&INPIT