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1. 发明专利

JPH04278073A PREPARATION OF RAW MATERIAL RICE FOR BREWING AND BREWING THEREOF 失效
公开(公告)号：JPH04278073A
公开(公告)日：1992-10-02
申请号：JP3626991
申请日：1991-03-01
申请人： GEKKEIKAN KK , HITACHI PLANT ENG & CONSTR CO
发明人： KAWATO SHOJI , ABE YASUHISA , ONO YOICHIRO , ITO MASAMITSU , YAMADERA TOSHIO
IPC分类号： B01D11/00 , C12G3/02
摘要： PURPOSE:To effectively remove lipid without eliminating a large amount of raw material rice for brewing by mechanical rice cleaning by subjecting raw material rice for brewing as it is or after any process of rice polishing, cleaning, immersing or steaming to specific extracting operation. CONSTITUTION:Raw material rice for brewing as it is or after any process of rice polishing, cleaning, immersing or steaming is brought into contact with carbon dioxide in a liquid or supercritical state and extracted so that saccharide attached to the surface of the raw material rice in rice polishing are removed and extraction efficiency of lipid is improved with carbon dioxide. The cleaning and immersing operations are preferably carried out by using water processed with an alkali or an acid and the extracting operation is done by bringing carbon dioxide in an upward countercurrent into contact with the raw material rice for brewing packed into a container.

2. 发明专利

JP2003164278A GENE OF D-AMINO ACID OXIDASE (DAOB) 审中-公开
公开(公告)号：JP2003164278A
公开(公告)日：2003-06-10
申请号：JP2001365305
申请日：2001-11-29
申请人： GEKKEIKAN KK
发明人： OBATA HIROSHI , HATA YOJI , SUGINAMI KOJI , ABE YASUHISA
IPC分类号： C12N15/09 , C12N1/19 , C12N9/06 , C12P41/00 , C12Q1/26
摘要： PROBLEM TO BE SOLVED: To mass-produce a new D-amino acid oxidase (DAOB) having activities for oxidizing a D-amino acid, and a new amino acid sequence (364 amino acid residues) by culturing a transformer. SOLUTION: A gene encoding the new DAOB is cloned, and the base sequence (2152bp) is determined. The cloned gene of the DAOB is inserted into a vector, and a host (Aspergillus oryzae) is transformed by using the obtained expression plasmid. COPYRIGHT: (C)2003,JPO

3. 发明专利

JP2002360262A ORNITHINE N5-OXYGENASE GENE WHICH PARTICIPATE IN BIOSYNTHESIS OF FERRICHROME OF ASPERGILLUS ORYZAE OR DEFERRI SUBSTANCE THEREOF 失效
公开(公告)号：JP2002360262A
公开(公告)日：2002-12-17
申请号：JP2001176264
申请日：2001-06-11
申请人： GEKKEIKAN KK
发明人： ISHIDA HIROKI , HATA YOJI , KAWATO SHOJI , SUGINAMI KOJI , ABE YASUHISA , IMAYASU SATOSHI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/06 , C12P21/02 , C12R1/69
摘要： PROBLEM TO BE SOLVED: To enable high production or non-production of ferrichrome of Aspergillus oryzae or deferi substance thereof under limitation of iron, enable modification of production of ferrichromes according to applications in molecular level and enable application to various industrial fields by using ornithine N5- oxygenase gene. SOLUTION: Cloning of a gene encoding ornithine N5-oxygenase protein which participates in biosynthesis of ferrichrome of Aspergillus oryzae or deferri substance thereof is carried out and a base sequence thereof is determined. It is confirmed that ornithine N5-oxygenase catalyzing a first stage of biosynthesis of ferrichrome or deferi substance thereof is encoded in gene fragment asb1 cloned, by the present invention.

4. 发明专利

JP2002306187A CYTOCHROME P450 nor GENE 审中-公开
公开(公告)号：JP2002306187A
公开(公告)日：2002-10-22
申请号：JP2001386472
申请日：2001-12-19
申请人： NAT RES INST OF BREWING , GEKKEIKAN KK
发明人： KAYA MASAHIKO , HATA YOJI , KAWATO SHOJI , ABE YASUHISA , AKITA OSAMU
IPC分类号： G01N33/50 , C12N1/14 , C12N1/15 , C12N1/20 , C12N9/02 , C12N15/09 , C12Q1/26 , C12R1/19 , C12R1/69 , G01N33/84
摘要： PROBLEM TO BE SOLVED: To enable to mass-produce cytochrome P450 nor-protein by culturing the obtained transformed product even in the case of using either gene in which the promoter is a long type or a shortened type and use the mass-produced enzyme in various industrial fields such as NO assay, NO scavenger in living organisms and the like. SOLUTION: A cloning of two kinds of genes coding for cytochrome P450 nor-protein is carried out and the total base sequence including their promoter parts, coding regions and terminators is determined. A transformed product is obtained by inserting the aforesaid novel genes treated by the cloning in a vector derived from Aspergillus oryzae and introducing the obtained manifested plasmid in host microbes.

5. 发明专利

JP2002272477A GENE ENCODING TRANSCRIPTION FACTOR CONTROLLING EXPRESSION OF FERRICHROME BIOSYNTHESIS GENE OF ASPERGILLUS 失效
公开(公告)号：JP2002272477A
公开(公告)日：2002-09-24
申请号：JP2001083640
申请日：2001-03-22
申请人： GEKKEIKAN KK
发明人： ISHIDA HIROKI , HATA YOJI , KAWATO SHOJI , ABE YASUHISA , IMAYASU SATOSHI
IPC分类号： C12N15/09 , C07K7/64 , C07K16/14 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P21/04 , C12R1/19 , C12R1/69
摘要： PROBLEM TO BE SOLVED: To provide confirmation that a transcription control factor negatively controlling ferrichrome biosynthesis is encoded in a gene fragment sreO cloned in the invention, possibility of enhanced production or non-production of the ferrichrome biosynthesis of Aspergillus under the limitation of iron using the gene, possibility of modification in molecular level of ferrichrome production in response to usage, and applicability of the gene in various industrial fields. SOLUTION: A gene encoding transcription factor controlling expression of ferrichrysin biosynthesis gene of Aspergillus is cloned and the base sequence is determined.

6. 发明专利

JP2002191355A BREEDING OF STRAIN HIGHLY PRODUCING ISOAMYL ACETATE, AND APPLICATION THEREOF 失效
公开(公告)号：JP2002191355A
公开(公告)日：2002-07-09
申请号：JP2000397797
申请日：2000-12-27
申请人： GEKKEIKAN KK
发明人： TSUTSUMI HIROKO , NAMISE MASAHIRO , OSHIMA TAKEHIKO , MINAZU TETSUYOSHI , KAWATO SHOJI , ABE YASUHISA
IPC分类号： C12G3/02 , C12C11/02 , C12N1/16 , C12N9/10 , C12R1/85
摘要： PROBLEM TO BE SOLVED: To provide a method for breeding a strain by which a high-quality alcohol drink containing a large amount of isoamyl acetate, and having excellent fragrance can be produced. SOLUTION: A mutated yeast formed by mutation, having high alcohol acetyl transferase activities, and high ratio of isoamyl acetate/isoamyl alcohol, capable of producing a large amount of the isoamyl acetate is bred and isolated. The novel mutated yeast can be obtained from the strain grown in a selective medium containing a steroid having a pregnane skeleton having a hydroxylated 3-position such as pregnenolone, 17-hydroxypregnenolone and pregnanediol, or a sterol such as cholesterol.

7. 发明专利

JP2002136290A AMINE OXIDASE GENE 失效
公开(公告)号：JP2002136290A
公开(公告)日：2002-05-14
申请号：JP2000336664
申请日：2000-11-02
申请人： NAT TAX ADMINISTRATION AGENCY , GEKKEIKAN KK
发明人： HISADA HIROMOTO , HATA YOJI , ABE YASUHISA , GOTO KUNIYASU
IPC分类号： C12N15/09 , C12N1/15 , C12N9/06 , C12R1/69
摘要： PROBLEM TO BE SOLVED: To enable the mass production of an amine oxidase by culturing a transformant containing an amine oxidase gene. SOLUTION: The amine oxidase gene is cloned, and the base sequence of the gene is determined. The new cloned gene is inserted into a vector to transform a host (aspergillus).

8. 发明专利

JPH04197171A DEVICE FOR PREPARING KOJI 失效
公开(公告)号：JPH04197171A
公开(公告)日：1992-07-16
申请号：JP32651290
申请日：1990-11-28
申请人： GEKKEIKAN KK
发明人： ABE YASUHISA , NISHIYAMA FUMIO , KASAI ISAMU , HATTORI SUSUMU
IPC分类号： C12M1/16
摘要： PURPOSE:To provide the subject device capable of controlling the temperature of a koji (malted rice) raw material and the evaporation of water by opening a closable hole-having membrane when air is ventilated or when a maintenance operation is performed, by spreading the closable hole-having membrane above a koji raw material layer formed on a koji supporter in a koji-preparing chamber. CONSTITUTION:A koji raw material layer is formed on a koji supporter 3 disposed in the koji-preparing chamber 6 of a koji-preparing tank 1, and while air in the koji-preparing chamber 6 is circulated by a dehumidifying means comprising a dehumidifying device 4, a dehumidifying fan and a circulation duct 7, the Aspergillus is cultured. When the koji raw material is maintained, etc., the closable hole-having membrane 9 disposed above the koji raw material layer 8 in the koji-preparing chamber 6 is opened, thereby permitting to freely control the evaporation of water from the koji raw material and the temperature of the koji raw material also due to the operation of the dehumidifying means.

9. 发明专利

JPH03175975A VARIANT YEAST AND ITS UTILIZATION 失效
公开(公告)号：JPH03175975A
公开(公告)日：1991-07-31
申请号：JP31540789
申请日：1989-12-06
申请人： GEKKEIKAN KK
发明人： AIKAWA MOTOYASU , ICHIKAWA EIJI , KAWATO SHOJI , ABE YASUHISA , IMAYASU SATOSHI
IPC分类号： C12P7/46 , A21D8/04 , C12C11/02 , C12G1/02 , C12G3/02 , C12N1/16 , C12R1/645
摘要： PURPOSE:To enable production of foods and drinks such as refined SAKE (Japanese rice wine) containing much malic acid by using a variant yeast obtained by efficient screening according to a method for selecting and obtaining thereof with dimethyl succinate. CONSTITUTION:A yeast such as refined SAKE yeast is subjected to variation treatment with ethyl methanesulfonate and then scattered on a YM plate to carry out culturing. Regenerated colonies are then replicated on a dimethyl succinate (DMS) plate. A strain without production is separated as a DMS- sensitive strain. Such strains are subsequently screened to provide a strain GHA-69 (FERM P-11080) capable of highly producing malic acid. The resultant variant yeast is capable of sufficiently producing organic acids in any of respective yeasts such as refined SAKE, 'SHOCHU' (popular distilled spirit), beer, wine and bread. If the variant strain is used to produce refined SAKE, etc., products containing much organic acids can be produced. Thereby, comfortable acid taste can be imparted to various foods and drinks in which the yeast participates.

10. 发明专利

JPH0292267A TREATMENT OF BREWAGE 失效
公开(公告)号：JPH0292267A
公开(公告)日：1990-04-03
申请号：JP24389288
申请日：1988-09-30
申请人： GEKKEIKAN KK
发明人： HATA YOJI , KAWATO SHOJI , ABE YASUHISA , ONO KAZUHISA
IPC分类号： C12G3/02 , C12H1/14
摘要： PURPOSE:To prevent deterioration of SAKE (rice wine) caused by occurrence of sweet dregs in brewage during preservation bringing glucoamylase in brewage into contact with a glucoamylase inhibitor to remove glucoamylase. CONSTITUTION:Refine a SAKE of brewage, for example, is passed through a column packed with an immobilized glucoamylase inhibitor to adsorb and remove glucoamylase in refine a SAKE. The glucoamylase inhibitor is an oligosaccharide shown by the formula containing alpha-1,4-imino bond in a molecule of acarbose produced by culture of actinomycetes and a relative substance thereof. A highly concentrated oligosaccharide of filtrate of a liquefied solution of polished rice (starch) is passed through a column packed with the above- mentioned inhibitor so that glucoamylase can be eluted from the inhibitor and recovered. On the other hand, refined SAKE of brewage from which glucoamylase is removed will not cause reduction in qualities even by preservation at room temperature.

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