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1. 发明专利

JP2014207884A キシロースイソメラーゼ活性を有するタンパク質及びその利用有权
标题翻译：具有XYLOSE异构体活性的蛋白质及其用途
公开(公告)号：JP2014207884A
公开(公告)日：2014-11-06
申请号：JP2014024878
申请日：2014-02-12
申请人：株式会社豊田中央研究所 , Toyota Central R&D Labs Inc , トヨタ自動車株式会社 , Toyota Motor Corp
发明人： KATAHIRA SATOSHI , NAGURA RISA , TOKUHIRO TATEO , ISHIDA NOBUHIRO , IMAMURA CHIE , HOYA NORIKO , TADA NOBUKI , ONISHI TORU
IPC分类号： C12N15/09 , C07K14/435 , C12N1/19 , C12N9/90 , C12P5/02 , C12P7/02 , C12P7/06 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/54 , C12P7/56 , C12P21/02
CPC分类号： C12N9/92 , C12N15/81 , C12P7/06 , C12Y503/01005 , Y02E50/17
摘要：【課題】キシロース消費能力及び発酵能力の高いキシロース資化能を有する酵母の提供。【解決手段】ヤマトシロアリの腸内原生生物由来のキシロースイソメラーゼ(XI)の特定の配列で表されるアミノ酸配列とアラインメントしたとき、それぞれ表される第1〜第6のモチーフをN末端側に近い側からのこの順序で有し、かつ前記第6のモチーフのアミノ酸配列におけるアスパラギン(N)に変えて他のアミノ酸を有するアミノ酸配列を有し、かつキシロースイソメラーゼ活性を有するタンパク質。【選択図】なし
摘要翻译：要解决的问题：提供具有木糖消耗能力和发酵能力的高木糖同化能力的酵母。解决方案：提供了在与由木糖异构酶的特定序列表示的氨基酸序列比对时的蛋白质（XI）衍生自具有第一至第六个基序的第一至第六个基序，其序列分别从N末端一侧依次表示，并具有氨基酸序列，其氨基酸序列具有用于天冬酰胺（N）的另一氨基酸氨基酸序列，并具有木糖异构酶活性。

2. 发明专利

JP2013169154A Polypeptide enhancing cellulosic biomass degradation activity 审中-公开
标题翻译：多糖增强细胞生物质降解活性
公开(公告)号：JP2013169154A
公开(公告)日：2013-09-02
申请号：JP2012033307
申请日：2012-02-17
申请人： Toyota Motor Corp , トヨタ自動車株式会社 , Toyota Central R&D Labs Inc , 株式会社豊田中央研究所 , Institute Of Physical & Chemical Research , 独立行政法人理化学研究所
发明人： SHISA TOMOKO , ISHIDA NOBUHIRO , IMAMURA CHIE , MORIYA SHIGEHARU , KIKUCHI ATSUSHI
IPC分类号： C12N15/09 , C07K14/37 , C12N1/19 , C12P7/06 , C12P19/14 , C12R1/865
CPC分类号： C12P7/14 , C07K14/37 , C12N9/2437 , C12P7/10 , C12P19/02 , C12P19/14 , Y02E50/16 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a polypeptide enhancing saccharification efficiency with a cellulase in a reaction temperature region of normal fermentation microorganisms except thermotolerant yeasts.SOLUTION: There are provided a polypeptide which is an isolated polypeptide derived from Neurospora crassa and containing a specific amino acid sequence, having a crystalline cellulose-binding ability and enhancing saccharification activity of a cellulase, a method for saccharification treatment in which cellulosic biomass is saccharified with a cellulase in the presence of the polypeptide and a method for producing alcohol by carrying out fermentation using a sugar component obtained by the treatment as a raw material.
摘要翻译：要解决的问题：提供在除了耐热酵母以外的正常发酵微生物的反应温度区域中纤维素酶增强糖化效率的多肽。解决方案：提供多肽，其是源于粗糙脉孢菌的分离多肽，并含有特定的氨基酸具有结晶纤维素结合能力和提高纤维素酶的糖化活性的方法，在多肽存在下纤维素生物质与纤维素酶糖化的糖化处理方法和通过使用糖进行发酵来制备醇的方法通过处理获得的成分作为原料。

3. 发明专利

JP2010041971A Variant formate dehydrogenase, gene encoding the same and method for producing nadh 审中-公开
标题翻译：变体脱氢酶，编码其的基因及其生产方法
公开(公告)号：JP2010041971A
公开(公告)日：2010-02-25
申请号：JP2008208975
申请日：2008-08-14
申请人： Toyota Central R&D Labs Inc , Toyota Motor Corp , トヨタ自動車株式会社 , 株式会社豊田中央研究所
发明人： KUSUMEGI YUKIHIRO , IMAMURA CHIE , TAKAHASHI HARUO
IPC分类号： C12N15/09 , C12N9/02 , C12P19/36
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide useful applications of a variant formate dehydrogenase by using a formate dehydrogenase having such high specific activity that can not be predicted from a conventional knowledge. SOLUTION: The formate dehydrogenase derived from Gibberella zeae is elucidated to show an outstandingly high specific activity among conventionally well-known formate dehydrogenases. A protein composed of an amino acid sequence shown, for example, by a sequence number 2 may be cited as the formate dehydrogenase derived from Gibberella zeae. NADH is produced by using the formate dehydrogenase derived from Gibberella zeae. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：通过使用具有这样高的比活性的甲酸脱氢酶来提供变体甲酸脱氢酶的有用应用，这是不能从常规知识预测的。解决方案：来源于玉米赤霉菌的甲酸脱氢酶被阐明，以显示在常规熟知的甲酸脱氢酶中具有非常高的比活性。由例如序列号2所示的氨基酸序列构成的蛋白质可以列举来自玉米赤霉菌（Gibberella zeae）的甲酸脱氢酶。通过使用源自玉米赤霉菌的甲酸脱氢酶生产NADH。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2014003970A Highly active mutant of protein having laccase activity, nucleic acid molecule encoding the same and use thereof 有权
标题翻译：具有乳酸酶活性的蛋白质的高活性突变体，编码其的核酸分子及其用途
公开(公告)号：JP2014003970A
公开(公告)日：2014-01-16
申请号：JP2013039643
申请日：2013-02-28
申请人： Aisin Seiki Co Ltd , アイシン精機株式会社 , Kazusa Dna Research Institute , 公益財団法人かずさDna研究所 , Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： SHIGEMORI YASUSHI , NAKAJIMA YASUO , YATABE MIYUKI , WATANABE TSUNEAKI , OISHI MICHIO , OBARA OSAMU , IMAMURA CHIE , KAJINO TSUTOMU , KAMIMURA KEIKO , UETAKA YUJI
IPC分类号： C12N15/09 , C12M1/34 , C12N9/02 , C12Q1/26 , G01N27/327 , H01M4/90 , H01M8/16
CPC分类号： Y02E60/527 , Y02P10/212
摘要： PROBLEM TO BE SOLVED: To provide a mutant having a substrate-oxidizing activity superior to that of the known proteins having laccase activity.SOLUTION: The protein is selected from the group consisting of: (a) proteins including an amino acid sequence in which methionine at position 308 of a specific amino acid sequence is replaced by another amino acid; and (b) proteins including an amino acid sequence in which one or more amino acids in a specific amino acid sequence are deleted or replaced or added, and the amino acid at position 308 of the specific amino acid sequence is not methionine, where the protein has an improved laccase activity in comparison to a protein having the specific amino acid sequence.
摘要翻译：要解决的问题：提供具有优于具有漆酶活性的已知蛋白质的底物氧化活性的突变体。溶液：所述蛋白质选自：（a）包括氨基酸序列的蛋白质，其中甲硫氨酸在特定氨基酸序列的308位被另一个氨基酸取代; 和（b）包含特定氨基酸序列中的一个或多个氨基酸被缺失或替换或添加的氨基酸序列的蛋白质，特定氨基酸序列的308位氨基酸不是甲硫氨酸，其中蛋白质与具有特定氨基酸序列的蛋白质相比，具有改善的漆酶活性。

5. 发明专利

JP2011182675A Cellulase composition and use of the same 审中-公开
标题翻译：纤维素组合物及其用途
公开(公告)号：JP2011182675A
公开(公告)日：2011-09-22
申请号：JP2010049427
申请日：2010-03-05
申请人： Gekkeikan Sake Co Ltd , Toyota Central R&D Labs Inc , 月桂冠株式会社 , 株式会社豊田中央研究所
发明人： IMAMURA CHIE , ISHIDA NOBUHIRO , IKEUCHI AKINORI , TAKAHASHI HARUO , MATSUYAMA TAKASHI , HISADA HIROMOTO , ISHIDA HIROKI , HATA YOJI
IPC分类号： C12N9/42 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10
摘要： PROBLEM TO BE SOLVED: To provide a cellulase composition including the combination of cellobiohydrolase (CBH) and endoglucanase (EG) which is suitable for the decomposition of cellulose.
SOLUTION: One or more first cellobiohydrolases (CBHs) belonging to GHF6, one or more second CBHs belonging to GHF7, one or more first endoglucanases (EGs) belonging to GHF5 and further one or more enzymes selected from the group consisting of (a) one or more second EGs belonging to GHF9, (b) one or more pectate lyases, (c) one or more xylanases, (d) one or more arabinofuranosidases, and (e) one or more glucuronoxylan xylanohydrolases are contained.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供包含纤维二糖水解酶（CBH）和内切葡聚糖酶（EG）的组合的纤维素酶组合物，其适用于纤维素的分解。解决方案：属于GHF6的一种或多种第一纤维二糖水解酶（CBH），属于GHF7的一种或多种第二种CBH，属于GHF5的一种或多种第一内切葡聚糖酶（EG）和另外一种或多种选自（ a）属于GHF9的一种或多种第二EG，（b）一种或多种果胶酸裂解酶，（c）一种或多种木聚糖酶，（d）一种或多种阿拉伯呋喃糖苷酶，和（e）一种或多种葡糖醛酸木聚糖水解酶。版权所有（C）2011，JPO＆INPIT

6. 发明专利

JP2009247324A Acid-resistant endoglucanase and use thereof 有权
标题翻译：耐酸内酰胺酶及其用途
公开(公告)号：JP2009247324A
公开(公告)日：2009-10-29
申请号：JP2008102744
申请日：2008-04-10
申请人： Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： MURAMOTO NOBUHIKO , IMAMURA CHIE , TOKUHIRO TATEO , TAKAHASHI HARUO
IPC分类号： C12N15/09 , C12N1/19 , C12N1/22 , C12N9/42 , C12P7/10 , C12P7/40 , C12Q1/40
CPC分类号： C12N9/2437 , C12P7/10 , C12P7/40 , C12P7/42 , C12Y302/01004 , Y02E50/16 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide an acid-resistant endoglucanase, i.e. a protein exhibiting excellent endoglucanase activity under acidic conditions and to provide effective use of the protein. SOLUTION: Provided are a protein composed of a specific amino acid sequence, an amino acid sequence having one or multiple amino acid mutations to the specific amino acid sequence or an amino acid sequence having homology of ≥75% to the specific amino acid sequence and having endoglucanase activity, and a protein having endoglucanase activity higher than that of endoglucanase derived from Trichoderma reesei at a pH of ≥2 and ≤4. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供耐酸内切葡聚糖酶，即在酸性条件下表现出优异的内切葡聚糖酶活性的蛋白质并提供该蛋白质的有效使用。解决方案：提供由特定氨基酸序列组成的蛋白质，对特定氨基酸序列具有一个或多个氨基酸突变的氨基酸序列或与特定氨基酸的同源性≥75％的氨基酸序列序列并具有内切葡聚糖酶活性，并且具有比在≥2和≤4的pH值高于里氏木霉的内切葡聚糖酶的内切葡聚糖酶活性的蛋白质。版权所有（C）2010，JPO＆INPIT

7. 发明专利

JP2009072102A Method for screening protein for degrading biomass 审中-公开
标题翻译：筛选蛋白质降解生物量的方法
公开(公告)号：JP2009072102A
公开(公告)日：2009-04-09
申请号：JP2007243626
申请日：2007-09-20
申请人： Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： IMAMURA CHIE , MURAMOTO NOBUHIKO , TAKAHASHI HARUO
IPC分类号： C12Q1/34 , C12M1/34
摘要： PROBLEM TO BE SOLVED: To provide an evaluation system for efficiently obtaining enzymes or a combination thereof which is effective for decomposing biomass.
SOLUTION: The method for screening the proteins for decomposing the biomass includes preparing a plurality of evaluation regions comprising solid bodies containing the biomass, of which decomposition is promoted by the combination of activities of a plurality of the proteins; supplying two or more kinds of subject proteins, having the possibility of constituting the combination in the plurality of the evaluation regions; and detecting the decomposition activities of the biomass by the two or more kinds of the subject proteins in a plurality of evaluation regions under a prescribed condition. As a result, the combination of proteins of enzymes. or the like. for decomposing the biomass by the synergistic action can be acquired efficiently.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种有效获得有效分解生物质的酶或其组合的评价系统。解决方案：用于筛选用于分解生物质的蛋白质的方法包括制备多个评估区域，其包含含有生物质的固体，其通过多种蛋白质的活性的组合促进分解; 提供两种或多种受试蛋白，具有在多个评价区域中构成组合的可能性; 以及在规定条件下，在多个评价区域中检测出两种以上的受试蛋白质的生物量的分解活性。因此，酶的蛋白质的组合。或类似物。用于通过协同作用分解生物质可以有效地获得。版权所有（C）2009，JPO＆INPIT

8. 发明专利

JP2008079564A Protein having xylose isomerase activity and use thereof 审中-公开
标题翻译：具有XYLOSE异构体活性的蛋白质及其用途
公开(公告)号：JP2008079564A
公开(公告)日：2008-04-10
申请号：JP2006265829
申请日：2006-09-28
申请人： Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： IMAMURA CHIE , TAKAHASHI HARUO
IPC分类号： C12N15/09 , C12N1/19 , C12N9/90 , C12P7/06 , C12P7/40 , C12P19/24
CPC分类号： Y02E50/17
摘要： PROBLEM TO BE SOLVED: To obtain a protein having an excellent activity of isomerizing xylose to xylulose (xylose-xylulose isomerization activity). SOLUTION: The wild type protein having xylose isomerase activity has an amino acid sequence having variations at positions corresponding to both or either of the 54 position and the 370 position of a sequence number 2. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：获得具有优异的将木糖异构化为木酮糖的功能的蛋白质（木糖 - 木酮糖异构化活性）。解决方案：具有木糖异构酶活性的野生型蛋白质具有在与序列号2的54位和370位相对应的位置具有变化的氨基酸序列。版权所有（C）2008 ，JPO＆INPIT

9. 发明专利

JP2012125154A Method for producing useful substance from cellulose-containing material and use thereof 审中-公开
标题翻译：从含有纤维素的材料生产有用物质的方法及其用途
公开(公告)号：JP2012125154A
公开(公告)日：2012-07-05
申请号：JP2010276690
申请日：2010-12-13
申请人： Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： KATAHIRA SATOSHI , NAKAMURA RISA , MORI TETSUYA , ISHIDA NOBUHIRO , IMAMURA CHIE , KODA KATSUNORI , TAKAHASHI HARUO
IPC分类号： C12P7/10 , C12N9/42 , C12N15/09
CPC分类号： Y02E50/16
摘要： PROBLEM TO BE SOLVED: To provide a treatment method of a cellulose-containing material more practical for decomposing a cellulose using an ionic liquid.SOLUTION: The method for producing a useful substance includes: a step of bringing the cellulose-containing material into contact with the ionic liquid to impregnate the cellulose-containing material with the ionic liquid; a step of bringing a solid phase that contains the cellulose-containing material into contact with an enzyme group including cellulase and xylanase to decompose the cellulose in the solid phase; and a step of producing the useful substance by the fermentation of microorganisms, using a carbon source that contains the cellulose decomposition product obtained by the cellulose decomposition process.
摘要翻译：要解决的问题：提供一种使用离子液体分解纤维素更实用的含纤维素材料的处理方法。解决方案：制备有用物质的方法包括：使含纤维素材料与离子液体接触以用离子液体浸渍含纤维素材料的步骤; 使包含含纤维素材料的固相与包含纤维素酶和木聚糖酶的酶组接触以使固相中的纤维素分解的步骤; 以及使用含有通过纤维素分解方法获得的纤维素分解产物的碳源，通过微生物的发酵来生产有用物质的步骤。版权所有（C）2012，JPO＆INPIT

10. 发明专利

JP2012039967A Heat-resistant cellobiohydrolase and use thereof 有权
标题翻译：耐热的碳水化合物及其用途
公开(公告)号：JP2012039967A
公开(公告)日：2012-03-01
申请号：JP2010185615
申请日：2010-08-20
申请人： Toyota Central R&D Labs Inc , 株式会社豊田中央研究所
发明人： ITO YOICHIRO , IMAMURA CHIE , IKEUCHI AKINORI
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N9/42 , C12P19/14
CPC分类号： Y02P20/52
摘要： PROBLEM TO BE SOLVED: To provide cellobiohydrolase II excellent in the heat resistance, and the use thereof.SOLUTION: A protein with cellobiohydrolase activity includes an amino acid sequence with one or two amino acid substitutions selected from kinds of mutation at 16 positions in the amino acid sequence composed of a specific sequence.

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