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    • 83. 发明专利
    • Gene encoding proteinase (a) and use thereof
    • 基因编码蛋白(A)及其使用
    • JP2009017783A
    • 2009-01-29
    • JP2005266075
    • 2005-09-13
    • Suntory Ltdサントリー株式会社
    • NAKAO YOSHIHIROKODAMA YUKIKOFUJIMURA TOMOKO
    • C12N15/09C12C11/02C12N1/19C12N9/60C12Q1/02C12Q1/37C12Q1/68C12R1/865
    • C12N9/60C12C12/004C12C12/006C12Q1/02
    • PROBLEM TO BE SOLVED: To provide a method for producing beer excellent in foam-retention property. SOLUTION: Provided is a polynucleotide described in one of the following (a) to (d). (a) A polynucleotide containing a polynucleotide comprising a specific base sequence originated from a beer yeast, (b) a polynucleotide containing a polynucleotide encoding a protein comprising a specific amino acid sequence originated from a beer yeast, (c) a polynucleotide containing a polynucleotide encoding a protein having an endo type peptidase activity and comprising an amino acid sequence in which one or more amino acids are deleted, replaced, inserted and/or added from/to a specific amino acid sequence which is originated from the beer yeast, and (d) a polynucleotide containing a polynucleotide which encodes a protein having an endo type peptidase activity and is hybridized, under a stringent condition, with a polynucleotide comprising a base sequence complementary to a specific base sequence originated from a beer yeast. COPYRIGHT: (C)2009,JPO&INPIT
    • 待解决的问题:提供一种生产泡沫保持性优异的啤酒的方法。 解决方案:提供以下(a)至(d)之一描述的多核苷酸。 (a)含有包含源自啤酒酵母的特异性碱基序列的多核苷酸的多核苷酸,(b)含有编码包含源自啤酒酵母的特定氨基酸序列的蛋白质的多核苷酸的多核苷酸,(c)含有多核苷酸的多核苷酸 编码具有内切型肽酶活性的蛋白质,并且包含从源自啤酒酵母的特定氨基酸序列缺失,替换,插入和/或添加一个或多个氨基酸的氨基酸序列,和 d)含有编码具有内切型肽酶活性的蛋白质的多核苷酸的多核苷酸,并且在严格条件下与包含与源自啤酒酵母的特异性碱基序列互补的碱基序列的多核苷酸杂交。 版权所有(C)2009,JPO&INPIT
    • 88. 发明专利
    • Method for detecting viable cell
    • 检测可见细胞的方法
    • JP2006068002A
    • 2006-03-16
    • JP2005222782
    • 2005-08-01
    • Fuji Electric Holdings Co Ltd富士電機ホールディングス株式会社
    • MIZUTANI TAKAAKINODA NAOHIRO
    • C12Q1/06G01N21/78G01N33/48
    • C12Q1/02
    • PROBLEM TO BE SOLVED: To provide a method for detecting viable cells by which the viable cells in a sample can rapidly, simply and accurately be detected and measured and further safety, preservation stability, cost properties, or the like, of a reagent are excellent.
      SOLUTION: The method for detecting the viable cells comprises the following steps. (1) a step of adding a fluorescent dye to the sample or bringing the sample into contact with the fluorescent dye and carrying out fluorescent staining of the cells, (2) a step of adding a quenching dye having properties of (a) permeating the cell membrane of the viable cells, (b) hardly absorbing the fluorescence of the fluorescent dye at a pH in the viable cells and (c) absorbing the fluorescence of the fluorescent dye at a pH different from the pH in the viable cells to the sample stained with the fluorescent dye under pH conditions different from the pH in the viable cells or bringing the sample into contact with the quenching dye, and (3) a step of irradiating the sample stained with the fluorescent dye and the quenching dye with excitation light for the fluorescent dye under pH conditions different from the pH in the viable cells, collecting and detecting the fluorescence emitted from the the sample. Thereby, the viable cells are detected.
      COPYRIGHT: (C)2006,JPO&NCIPI
    • 待解决的问题:提供一种用于检测样品中的活细胞的活细胞的方法,其可以快速,简单且准确地检测和测量,并进一步提高安全性,保存稳定性,成本性质等 试剂很好。 解决方案:用于检测活细胞的方法包括以下步骤。 (1)向样品中添加荧光染料或使样品与荧光染料接触并进行细胞的荧光染色的步骤,(2)添加具有(a)渗透到 活细胞的细胞膜,(b)在活细胞的pH下几乎不吸收荧光染料的荧光,和(c)在与活细胞中的pH不同的pH下吸收荧光染料的荧光 在不同于活细胞中的pH的pH条件下使荧光染料染色,或使样品与淬灭染料接触,以及(3)用荧光染料和淬灭染料用激发光照射样品的步骤 荧光染料在pH条件下与活细胞中的pH不同,收集和检测从样品发出的荧光。 从而检测活细胞。 版权所有(C)2006,JPO&NCIPI