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1. 发明专利

JP2009145260A Centrifuge separation method and centrifugal separator 有权
标题翻译：离心分离方法和离心分离器
公开(公告)号：JP2009145260A
公开(公告)日：2009-07-02
申请号：JP2007324538
申请日：2007-12-17
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： MIZUTANI TAKAAKI , NODA NAOHIRO
IPC分类号： G01N1/10 , B04B5/02 , G01N35/00
摘要： PROBLEM TO BE SOLVED: To provide a centrifuge separation method and centrifugal separator where mechanism and operation are simple when the centrifuge separation process is automatically performed by the separator, and to provide an easy centrifuge separation method and centrifugal separator for preventing evaporation of a sample under centrifuge separation without using a lid.
SOLUTION: This centrifuge separation method comprises a dispensing process of dispensing the sample 1 to a centrifugal tube 2, an adding process of adding evaporation preventing liquid 3 to the upper part of the sample 1 and superimposing it on the upper part of the sample 1, and a centrifuge separation process of rotating the centrifugal tube 2 to separate the material in the sample 1 with centrifugal force with the centrifugal separator 21.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供离心分离方法和离心分离机，其中当离心机分离过程由分离器自动进行时机构和操作简单，并提供容易的离心分离方法和用于防止蒸发的离心分离器在不使用盖子的情况下离心分离的样品。解决方案：该离心分离方法包括将样品1分配到离心管2的分配过程，添加防腐液3到样品1的上部并将其叠加在样品1的上部的添加过程样品1和离心分离过程，使离心管2旋转，以离心分离器21离心分离样品1中的材料。（C）2009年，JPO和INPIT

2. 发明专利

JP2006141351A Method for concentration of microorganism 有权
标题翻译：浓缩微生物的方法
公开(公告)号：JP2006141351A
公开(公告)日：2006-06-08
申请号：JP2004339448
申请日：2004-11-24
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： NODA NAOHIRO , HARADA MAIKO , IGARASHI TOSHIO
IPC分类号： C12N1/00 , C12N1/02
摘要： PROBLEM TO BE SOLVED: To provide a method for concentrating microorganism in a high magnification, a high recovery without concentrating water soluble foreign substances such as a starch and a protein, etc., and highly hydrophilic foreign substances.
SOLUTION: The method comprises formation of a homogeneous solution by adding a surfactant and a hydrophilic organic solvent to a solution containing the microorganism and reducing the pH of the solution allowing separation of the solution into two phases of a deposited phase including a mixture of the microorganism, the surfactant, the hydrophilic organic solvent and water in a mixed state and a supernatant liquid phase, and then concentrating the microorganisms in the separated phase.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种以高放大倍数浓缩微生物的方法，高回收率而不浓缩水溶性异物如淀粉和蛋白质等，以及高度亲水的异物。解决方案：该方法包括通过向含有微生物的溶液中加入表面活性剂和亲水性有机溶剂来形成均匀溶液，并降低溶液的pH，使溶液分离成包括混合物的沉积相的两相的微生物，表面活性剂，亲水性有机溶剂和混合状态的水和上清液相，然后将微生物浓缩成分离相。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2006068002A Method for detecting viable cell 有权
标题翻译：检测可见细胞的方法
公开(公告)号：JP2006068002A
公开(公告)日：2006-03-16
申请号：JP2005222782
申请日：2005-08-01
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： MIZUTANI TAKAAKI , NODA NAOHIRO
IPC分类号： C12Q1/06 , G01N21/78 , G01N33/48
CPC分类号： C12Q1/02
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting viable cells by which the viable cells in a sample can rapidly, simply and accurately be detected and measured and further safety, preservation stability, cost properties, or the like, of a reagent are excellent.
SOLUTION: The method for detecting the viable cells comprises the following steps. (1) a step of adding a fluorescent dye to the sample or bringing the sample into contact with the fluorescent dye and carrying out fluorescent staining of the cells, (2) a step of adding a quenching dye having properties of (a) permeating the cell membrane of the viable cells, (b) hardly absorbing the fluorescence of the fluorescent dye at a pH in the viable cells and (c) absorbing the fluorescence of the fluorescent dye at a pH different from the pH in the viable cells to the sample stained with the fluorescent dye under pH conditions different from the pH in the viable cells or bringing the sample into contact with the quenching dye, and (3) a step of irradiating the sample stained with the fluorescent dye and the quenching dye with excitation light for the fluorescent dye under pH conditions different from the pH in the viable cells, collecting and detecting the fluorescence emitted from the the sample. Thereby, the viable cells are detected.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：待解决的问题：提供一种用于检测样品中的活细胞的活细胞的方法，其可以快速，简单且准确地检测和测量，并进一步提高安全性，保存稳定性，成本性质等试剂很好。解决方案：用于检测活细胞的方法包括以下步骤。（1）向样品中添加荧光染料或使样品与荧光染料接触并进行细胞的荧光染色的步骤，（2）添加具有（a）渗透到活细胞的细胞膜，（b）在活细胞的pH下几乎不吸收荧光染料的荧光，和（c）在与活细胞中的pH不同的pH下吸收荧光染料的荧光在不同于活细胞中的pH的pH条件下使荧光染料染色，或使样品与淬灭染料接触，以及（3）用荧光染料和淬灭染料用激发光照射样品的步骤荧光染料在pH条件下与活细胞中的pH不同，收集和检测从样品发出的荧光。从而检测活细胞。版权所有（C）2006，JPO＆NCIPI

4. 发明专利

JP2008256701A Antigen separator, and antigen measuring method and device using antgen separator 有权
标题翻译：抗原分离器和抗原测量方法和使用安培分离器的装置
公开(公告)号：JP2008256701A
公开(公告)日：2008-10-23
申请号：JP2008114794
申请日：2008-04-25
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： NODA NAOHIRO , YOKOYAMA KATSUJI , TANAKA YOSHIHARU , SHIMIZU HIDEO
IPC分类号： G01N33/569 , B01D57/02 , B03C5/00 , G01N21/78 , G01N33/48 , G01N33/53 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To shorten antigen measuring time, and to enable highly accurate measurement, by measuring an image of an antigen separated by using this separator, by providing the antigen separator capable of securing a fluid body processing quantity corresponding to the necessity, by solving the problem of dust clogging when separating the antigen.
SOLUTION: This antigen separator has an introducing port 1 of a sample fluid including the antigen, a reaction part 3 separating the antigen by antigen/antibody reaction by arranging a plurality of antibody beads on a bottom surface in a microchannel having a rectangular cross section for flowing the sample fluid, a sample fluid introducing passage 4 communicating the introducing port with the reaction part, and a discharge passage 5 of the sample fluid. The introducing passage has an air introducing port 11, and comprises the antigen enriching function by layer separation of an air layer and a sample fluid layer in the vertical direction to the bottom surface by simultaneously introducing the sample fluid and air in the same horizontal plane. An image measuring device is provided above the reaction part.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：为了缩短抗原测定时间，通过测定通过使用该隔膜分离的抗原的图像，能够进行高精度的测定，通过提供能够确保对应于必要时，通过解决分离抗原时灰尘堵塞的问题。解决方案：该抗原分离器具有包含抗原的样品流体的引入口1，反应部分3通过在具有矩形的微通道的底表面上布置多个抗体珠，通过抗原/抗体反应分离抗原用于流动样品流体的横截面，将引入口与反应部分连通的样品流体引入通道4和样品流体的排出通道5。引入通道具有空气引入口11，并且通过同时将样品流体和空气引入同一水平面，通过层压分离空气层和样品流体层而在垂直方向上包含抗原富集功能。在反应部分上方设置有图像测量装置。版权所有（C）2009，JPO＆INPIT

5. 发明专利

JP2007006880A Method for measuring enzyme and apparatus for measuring the same 有权
标题翻译：用于测量酶的装置的方法和装置
公开(公告)号：JP2007006880A
公开(公告)日：2007-01-18
申请号：JP2005261745
申请日：2005-09-09
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： IGARASHI TOSHIO , KATO JUN , NODA NAOHIRO
IPC分类号： C12Q1/25 , C12M1/34 , G01N21/80
摘要： PROBLEM TO BE SOLVED: To provide a method for measuring an enzyme, capable of giving a result on the spot, without requiring a waiting time due to an induction period, capable of continuously collecting and measuring a sample, and capable of following a change of a concentration of the enzyme in the sample with the lapse of time, and to provide an apparatus for measuring the same.
SOLUTION: This method for measuring the enzyme comprises utilizing autocatalytic reaction in which the enzyme acts as a catalyst and calculating an amount of the enzyme contained in a sample solution based on a time by which the reaction has rapidly proceeded, wherein the sample solution is mixed with a reagent participating in the autocatalytic reaction, so as to form a mixed solution, the mixed solution is poured into a channel at a prescribed flow rate, the autocatalytic reaction is made to arise in the channel, and the amount of the enzyme contained in the sample solution is calculated based on a state of a change of a color developed in the channel in accordance with the autocatalytic reaction.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种能够在现场得到结果的酶的测定方法，而不需要由于诱导期的等待时间，能够连续收集和测量样品，并能够追踪随着时间的推移使样品中的酶浓度发生变化，并提供用于测量该装置的装置。解决方案：用于测量酶的方法包括利用自动催化反应，其中酶用作催化剂并基于反应快速进行的时间计算样品溶液中所含的酶的量，其中样品溶液与参与自催化反应的试剂混合，以形成混合溶液，将混合溶液以规定的流速倒入通道中，使通道中出现自催化反应，基于根据自动催化反应在通道中显影的颜色的变化的状态计算样品溶液中所含的酶。版权所有（C）2007，JPO＆INPIT

6. 发明专利

JP2006158266A Measurement method of microorganism 有权
标题翻译： MICROORGANISM的测量方法
公开(公告)号：JP2006158266A
公开(公告)日：2006-06-22
申请号：JP2004352825
申请日：2004-12-06
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： NODA NAOHIRO , HARADA MAIKO , IGARASHI TOSHIO
IPC分类号： C12Q1/06 , C12N15/09 , C12Q1/28 , C12Q1/68 , C12Q1/70
摘要： PROBLEM TO BE SOLVED: To provide a method of microorganism measurement, using a new signal amplification means to realize a highly sensitive detection. SOLUTION: The method comprises introducing or bonding an enzyme to a microorganism to be measured, or synthesizing the enzyme in the microorganism, measuring the enzyme amount by autocatalytic reaction and calculating the enzyme amount from the amount of the measured enzyme. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：为了提供微生物测量的方法，使用新的信号放大装置来实现高灵敏度检测。解决方案：该方法包括向待测微生物引入或结合酶，或在微生物中合成酶，通过自动催化反应测定酶量，并从测定的酶的量计算酶量。版权所有（C）2006，JPO＆NCIPI

7. 发明专利

JP2005241435A Method and apparatus for counting live cells 审中-公开
标题翻译：用于计数活细胞的方法和装置
公开(公告)号：JP2005241435A
公开(公告)日：2005-09-08
申请号：JP2004051613
申请日：2004-02-26
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： SANO YASUKAZU , NODA NAOHIRO , SHIMIZU HIDEO
IPC分类号： G01N21/64 , C12M1/34 , C12Q1/06 , G01N33/48 , G01N33/58
摘要： PROBLEM TO BE SOLVED: To provide a live cell measuring method capable of simply measuring the number of live cells in a short time, and to provide a live cell measuring instrument made simple and small-sized.
SOLUTION: In the method for labelling bacteria 21 as live cells in a specimen with a fluorescent regent 21a to count the number of live cells, the bacteria 21 labelled with the fluorescent regent 21a in a reaction layer are exceted by the blue light of a light emitting diode 25 and the quantity of fluorescence (green light) emitted from the bacteria is measured by a photodiode 33 and, on the basis of the correlation of the number of preliminarily calculated bacteria per a unit area and the measured value of the quantity of fluorescence, the number of bacteria is operationally calculated. In the apparatus for counting the number of live cells, a dichroic mirror 31 is provided to the upper part of the reaction layer 24 in a bacterial flow channel 20 to make the inclined surfaces of two prisms back to back and the back-to-back surfaces of the prisms are formed so as to reflect blue light but to transmit green light to be integrated with the bacteria flow channel, an optical filter, the light emitting diode, the photodiode or the like.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供能够简单地在短时间内测量活细胞数量的活细胞测量方法，并提供简单小型的活细胞测量仪器。解决方案：在具有荧光光度计21a的样品中的活细胞标记细菌21的方法中，计数活细胞数，在反应层中用荧光试剂21a标记的细菌21被蓝光并且通过光电二极管33测量从细菌发射的荧光量（绿光），并且基于每单位面积的预先计算的细菌数与该单位面积的测定值的相关性荧光数量，细菌数量可操作计算。在用于计数活细胞数的装置中，在细菌流通道20中向反应层24的上部提供分色镜31，以使两个棱镜的倾斜表面背对背和背对背棱镜的表面形成为反射蓝光，而是透过与细菌流路一体化的绿光，滤光器，发光二极管，光电二极管等。版权所有（C）2005，JPO＆NCIPI

8. 发明专利

JP2005052059A Method and system for measuring microorganism or cell 有权
标题翻译：用于测量微生物或细胞的方法和系统
公开(公告)号：JP2005052059A
公开(公告)日：2005-03-03
申请号：JP2003285613
申请日：2003-08-04
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： NODA NAOHIRO , ONODERA TAKUYA , ASANO TAKAMASA
IPC分类号： G01N33/48 , C12M1/34 , C12Q1/06 , G01N33/483
摘要： PROBLEM TO BE SOLVED: To provide a method and a system for measuring the kind( or both kind and number ) of microorganisms or cells in a shorter time depending on no use of any selective medium.
SOLUTION: The method comprises the step (1) of inoculating an agar medium with microorganisms or cells as a measurement sample( hereafter referred to as an initial sample ), the step (2) of forming the colony or culture-grown product of the initial sample( hereafter referred to as colony en bloc ) and detecting the shape of a grown product at a stage prior to the colony's growing to a visible size( hereafter referred to as a microcolony ) and the step (3) of identifying the kind of the initial sample based on the detection result of the microcolony's shape. Optionally, the initial shape of the microcolony, the time to arrival for enabling detection as the microcolony, or the like, are also served as identification requirement(s).
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供用于根据不使用任何选择性介质在较短时间内测量微生物或细胞的种类（或类型和数量）的种类（或种类和数量）的方法和系统。解决方案：该方法包括将作为测量样品的微生物或细胞的琼脂培养基（以下称为初始样品）接种的步骤（1），形成菌落或培养生长产物的步骤（2）的初始样品（以下简称为集落），并且在菌落生长至可见尺寸之前的阶段检测生长产物的形状（以下称为微菌落）和步骤（3）基于小菌落形状的检测结果，初始样品的种类。可选地，微菌落的初始形状，用于使检测作为微菌落的到达时间等也被用作识别要求。版权所有（C）2005，JPO＆NCIPI

9. 发明专利

JP2004233114A Antigen separator, and method and instrument for measuring antigen by using the same 有权
公开(公告)号：JP2004233114A
公开(公告)日：2004-08-19
申请号：JP2003019752
申请日：2003-01-29
申请人： Fuji Electric Holdings Co Ltd , 富士電機ホールディングス株式会社
发明人： NODA NAOHIRO , YOKOYAMA KATSUJI , TANAKA YOSHIHARU , SHIMIZU HIDEO
IPC分类号： G01N33/557 , G01N21/77 , G01N21/78 , G01N33/543 , G01N35/08 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide an antigen separator for eliminating a problem of foreign matter clogging in antigen separation and securing the amount of treated fluid meeting the need, and to perform the shortening of antigen measurement time and high-accuracy measurement by performing image measurement on antigens separated by using the separator.
SOLUTION: This antigen separator is equipped with a specimen inlet 1 for a specimen fluid including antigens, a reaction part 3 used for separating the antigens by antigen/antibody reaction and made by disposing a plurality of antibody beads 2 on a bottom surface of a micro-channel having a rectangular cross-section through which the specimen fluid flows, a specimen-liquid inlet path 4 for causing the inlet to communicate with the reaction part, and an exhaust path 5 for the specimen fluid. The inlet path 4 is substantially equipped with a difference 4a in flow path level from the reaction part so that it is equipped with an antigen condensing function in the vicinity of the bottom surface, and/or, the reaction part 3 is equipped with an electric field generating means 30 for attracting the antigens by an electric attracting force to the bottom surface side where the antibody beads are disposed. An image measuring instrument is provided above the reaction part.
COPYRIGHT: (C)2004,JPO&NCIPI

10. 发明专利

JP2008035788A Pretreatment method of sample for measuring number of microorganism, pretreatment kit and pretreatment apparatus 审中-公开
标题翻译：用于测量微生物数量，预处理工具包和预处理设备的样品预处理方法
公开(公告)号：JP2008035788A
公开(公告)日：2008-02-21
申请号：JP2006214889
申请日：2006-08-07
申请人： Fuji Electric Holdings Co Ltd , Nisshin Seifun Group Inc , 富士電機ホールディングス株式会社 , 株式会社日清製粉グループ本社
发明人： IMAI SHINJIRO , TANAKA KEIKO , NODA NAOHIRO
IPC分类号： C12Q1/06 , G01N21/78
摘要： PROBLEM TO BE SOLVED: To provide a pretreatment method of a sample for measuring the number of microorganisms, with which a sample-derived determination inhibiting component, especially an insoluble component is effectively removed.
SOLUTION: A protein existing in a sample is dissolved in an alkali buffer solution, the dissolved protein is removed from the sample and the sample is used for the rapid measurement of the number of microorganisms.
COPYRIGHT: (C)2008,JPO&INPIT

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