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31. 发明专利

JP2005254205A Method and apparatus for treating female hormone in sewage 审中-公开
标题翻译：用于处理女性HORMONE的方法和装置
公开(公告)号：JP2005254205A
公开(公告)日：2005-09-22
申请号：JP2004073156
申请日：2004-03-15
申请人： Ebara Corp , Japan Sewage Works Agency , 日本下水道事業団 , 株式会社荏原製作所
发明人： ONDA KENSUKE , TADA KEITARO , MIYA AKIKO , HASHIMOTO TOSHIICHI , MISHINA FUMIO
IPC分类号： C12N1/20 , C02F3/12 , C02F3/34
CPC分类号： Y02W10/15
摘要： PROBLEM TO BE SOLVED: To provide a method which can remove a female hormone in sewage to a low-concentration level in an efficient and stable manner. SOLUTION: In this method, the concentration of female hormone degradation microorganisms in activated sludge or the like is measured. The operation method for a treatment apparatus is controlled according to the measured concentration of female hormone degradation microorganisms to maintain the concentration of the female hormone degradation microorganisms in the activated sludge or the like at a given level or higher. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种能够以有效和稳定的方式将污水中的雌激素去除至低浓度水平的方法。解决方案：在该方法中，测定活性污泥等中的雌激素降解微生物的浓度。根据测定的雌性激素降解微生物的浓度来控制处理装置的操作方法，以将给定水平以上的活性污泥等中的雌激素降解微生物的浓度维持在一起。版权所有（C）2005，JPO＆NCIPI

32. 发明专利

JP2005246119A Anaerobic treating method for polluted material containing oil and fat, and device therefor 有权
标题翻译：包含油和脂肪的污染物质的厌氧处理方法及其装置
公开(公告)号：JP2005246119A
公开(公告)日：2005-09-15
申请号：JP2004055996
申请日：2004-03-01
申请人： Ebara Corp , 株式会社荏原製作所
发明人： HAO RINYUN , KATAOKA NAOAKI , MIYA AKIKO
IPC分类号： C12M1/00 , C02F1/00 , C02F3/28 , C02F11/04 , C12M1/107 , C12M1/34 , C12M1/36 , C12M1/38 , C12N1/00
CPC分类号： Y02E50/343
摘要： PROBLEM TO BE SOLVED: To provide a cleaning method capable of steadily and efficiently performing the anaerobic treatment of oils and fats containing wastewater and oils and fats containing waste by optimally controlling a methane fermentation reaction which is a total result of co-work by many kinds of microorganisms, and to provide a cleaning process and a cleaning device used for the cleaning method. SOLUTION: In one embodiment of this invention, the anaerobic treating process of the oils and fats containing polluted materials is characterized in that the concentration of propionic acid in sludge in a reactor is monitored and that fumaric acid is added according to the monitored concentration of propionic acid in the anaerobic treating process of the oils and fats containing polluted materials. COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：要解决的问题：提供一种清洁方法，其能够通过最佳地控制作为共同作用的总体结果的甲烷发酵反应来稳定和有效地进行含有废水和含有废物的油脂的油和脂肪的厌氧处理通过多种微生物，并提供清洁方法和用于清洁方法的清洁装置。解决方案：在本发明的一个实施方案中，含有污染物质的油和脂肪的厌氧处理过程的特征在于监测反应器中污泥中丙酸的浓度，并根据所监测的添加富马酸在含有污染物质的油和脂肪的厌氧处理过程中丙酸的浓度。版权所有（C）2005，JPO＆NCIPI

33. 发明专利

JP2004342412A Power generation method and device using organic substance 审中-公开
公开(公告)号：JP2004342412A
公开(公告)日：2004-12-02
申请号：JP2003136164
申请日：2003-05-14
申请人： Ebara Corp , 株式会社荏原製作所
发明人： SHIMOMURA TATSUO , YOKOTA HIROSHI , YAKUWA HIROSHI , MIYA AKIKO
IPC分类号： B09B3/00 , C02F3/28 , C02F3/34 , C02F11/04 , H01M8/16 , H01M14/00
CPC分类号： Y02E60/527
摘要： PROBLEM TO BE SOLVED: To provide a method of efficiently obtaining electric energy from an aqueous organic substance with a simple device. SOLUTION: In the power generating method, one of the pair of electrodes as an anode is brought into contact with solution or slurry containing viable anaerobic microbes and an organic substance, and the other as a cathode is formed at least partly by a conductive porous material having pores in a structure, mesh or fibrous material. Then, the cathode is brought into contact with air with a contact interface of the air and water existent in the pores of the cathode, the cathode is separated from the solution or the slurry through an electrolyte film, and the cathode and the anode are electrically connected to form a closed circuit, whereby, a reduction reaction is made to proceed in the cathode with oxygen as an electron acceptor, and an oxidation reaction by the microbes is made to proceed in the anode with the organic substance as an electron donor. COPYRIGHT: (C)2005,JPO&NCIPI

34. 发明专利

JP2004267099A Method for detecting escherichia coli and phage for detecting escherichia coli 有权
标题翻译：用于检测ESCHERICHIA COLI和PHAGE的检测方法
公开(公告)号：JP2004267099A
公开(公告)日：2004-09-30
申请号：JP2003062440
申请日：2003-03-07
申请人： Ebara Corp , Rikogaku Shinkokai , 株式会社荏原製作所 , 財団法人理工学振興会
发明人： TANJI YASUNORI , UNNO HAJIME , FURUKAWA CHIAKI , ONDA KENSUKE , MIYA AKIKO , KENMOCHI YUKIO
IPC分类号： C12N15/09 , C07K14/01 , C12N7/00 , C12N7/01 , C12Q1/02 , C12Q1/06 , C12Q1/10 , C12Q1/68 , C12Q1/70
CPC分类号： C12N7/00 , C07K14/005 , C07K2319/60 , C12N2795/10122 , C12Q1/10 , C12Q1/689
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting Escherichia coli with extremely improved sensitivity by utilizing a phage by which the decrease of sensitivity caused by bacteriolysis is reduced, and the live bacteria can be specifically detected. SOLUTION: The method for detecting the Escherichia coli involves detecting the Escherichia coli present in a specimen by bringing the phage infecting specified Escherichia coli into contact with the specimen. The method comprises using the phage the coat of which has a fluorescent protein expressed therein as a protein fused with the coat protein, and which has defects in a lytic enzyme. Particulary, the method comprises detecting the fluorescence at an intensity level emitted from the fluorescent protein of the phage adsorbed on the Escherichia coli, and/or detecting the fluorescence at the intensity level emitted from the fluorescent protein expressed in the Escherichia coli caused by the adsorption of the phage. COPYRIGHT: (C)2004,JPO&NCIPI
摘要翻译：要解决的问题：提供一种通过利用由细菌溶解引起的敏感性降低降低的噬菌体来检测具有极高的灵敏度的大肠杆菌的方法，并且可以特异性地检测活细菌。解决方案：用于检测大肠杆菌的方法包括通过使感染指定的大肠杆菌的噬菌体与样品接触来检测样品中存在的大肠杆菌。该方法包括使用其外含有其中表达的荧光蛋白的噬菌体作为与外壳蛋白融合的蛋白质，并且在裂解酶中具有缺陷。所述方法包括从吸附在大肠杆菌上的噬菌体的荧光蛋白发射的强度水平检测荧光，和/或从由吸附引起的大肠杆菌中表达的荧光蛋白发射的强度水平检测荧光的噬菌体。版权所有（C）2004，JPO＆NCIPI

35. 发明专利

JP2004261124A Primer for detecting methanogen, detecting method using the same, etc. 有权
公开(公告)号：JP2004261124A
公开(公告)日：2004-09-24
申请号：JP2003056081
申请日：2003-03-03
申请人： Ebara Corp , Univ Nihon , 学校法人日本大学 , 株式会社荏原製作所
发明人： BEPPU TERUHIKO , HOSONO KUNIAKI , UEDA KENJI , MIYA AKIKO , KATAOKA NAOAKI
IPC分类号： G01N33/483 , C02F1/00 , C12N15/09 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/569
摘要： PROBLEM TO BE SOLVED: To provide a method for exhaustively detecting methanogens in an environmental specimen such as methane fermentation sludge and provide a primer for the detection method. SOLUTION: The method for the detection of methanogens in a nucleic acid specimen derived from methanogens in an environmental specimen comprises a PCR method using either one of the following primer sets consisting of (a) an oligonucleotide having the 1st specific base sequence or a base sequence essentially homologous to the 1st sequence and an oligonucleotide having the 2nd specific base sequence or a base sequence essentially homologous to the 2nd sequence and (b) an oligonucleotide having the 3rd specific base sequence or a base sequence essentially homologous to the 3rd sequence and an oligonucleotide having the 4th specific base sequence or a base sequence essentially homologous to the 4th sequence, and the detection of the amplification product obtained by the PCR. COPYRIGHT: (C)2004,JPO&NCIPI

36. 发明专利

JP2000271593A ANAEROBIC TREATMENT METHOD OF GREASE-CONTAINING WASTE WATER 失效
公开(公告)号：JP2000271593A
公开(公告)日：2000-10-03
申请号：JP8000699
申请日：1999-03-24
申请人： EBARA CORP
发明人： WATANABE AKIRA , MIYA AKIKO , YONEYAMA YUTAKA , SUZUKI TAKAYUKI
IPC分类号： C02F1/00 , C02F1/58 , C02F3/28
摘要： PROBLEM TO BE SOLVED: To provide an anaerobic treatment method enhancing the decomposi tion rate of grease in waste water, capable of suppressing the formation of scum, increasing the formation amt. of biomass and dispensing with the disposal of grease. SOLUTION: Grease-containing waste water 1 is separated into a grease component 3 and separated water 4 and the separated grease component 3 is saponified and the formed grease saponified matter 7 (pref. an alkali metal salt of fatty acid, especially, a sodium salt thereof) is subjected to acid fermentation treatment along with the separated water 4 under a pH condition of 11 or less. An aic fermentation process pref. consists of a plurality of tanks 8, 9 different in pH and the pH of the first stage acid fermentation tank 8 is held to 9-11. Pref., methane fermentation is performed in a methane fermentation tank 10 after the acid fermentation process.

37. 发明专利

JPH08116992A EXAMINATION OF ANTIMICROBIAL PROPERTY 失效
公开(公告)号：JPH08116992A
公开(公告)日：1996-05-14
申请号：JP27865494
申请日：1994-10-19
申请人： EBARA CORP , MERCIAN CORP , MERUSHIYAN CLEAN TEC KK
发明人： MIYA AKIKO , SASAKI KENICHI , KAWAKAMI HISASHI , OKAMURA KAZUHIKO , SAKAMOTO MICHIKO
IPC分类号： C12Q1/18 , C12Q1/30 , C12Q1/37
摘要： PURPOSE: To provide an antimicrobial efhect-examining method capable of quantitatively judging ability to catch a microbial cell with a fibrous raw material and/or a product and ability to suppress proliferation of caught microbial cell. CONSTITUTION: This method for examining antimicrobial properties comprises combinedly using both the measurement of catching activity of microbial cell comprising dispersing a microbial cell attaching to a raw material and/or a product into a liquid culture medium or a buffer and recovering, cleaning and culturing the dispersed microbial cell and examining viable microbe numbers and the measurement of antimicrobial activity comprising measuring biochemical activity of a microorganism attached to the raw material and/or the product and examining survival property of the attached microorganism, in measuring an antimicrobial activity of the fibrous raw material and/or the product to judge antimicrobial activity of the raw material and/or the product. One or more biochemical activities selected from a catalase activity, an indole producing activity and a gelatin liquefying activity are preferably used as the biochemical activities.

38. 发明专利

JPH0775550A CELL CULTURE APPARATUS 失效
公开(公告)号：JPH0775550A
公开(公告)日：1995-03-20
申请号：JP24852193
申请日：1993-09-10
申请人： EBARA CORP
发明人： ADACHI TADASHI , TANIGUCHI SHIN , MIYA AKIKO
IPC分类号： C12M1/02 , C12M3/00
摘要： PURPOSE:To provide a cell culture tank which prevents the cells from being broken by a strong stress, can culture the cells growing in a fibrous form without pellet formation or lumping, and can prevent the cells immobilized to the support from being peeled off. CONSTITUTION:In a culture tank for mammalian or plant cells and/or cells of microorganisms, the culture tank is provided with a stirring mechanism which can arbitrarily vary the liquid flow rate and turn the flow direction. The mechanism is a cage type of rotor 2 in which the screw blades 10 fitted to the rotation shaft 3 for the rotor and the peripheral blades 9 have the structures to cause the flows reverse to each other. It is preferred that the cell culture installation is provided with a means for observation of the cell conditions and a system for controlling the flow rate and/or the flow direction of the stirring mechanism on the basis of the observation.

39. 发明专利

JP2010276499A Microchip electrophoretic apparatus 审中-公开
标题翻译： MICROCHIP电子装置
公开(公告)号：JP2010276499A
公开(公告)日：2010-12-09
申请号：JP2009129927
申请日：2009-05-29
申请人： Ebara Corp , 株式会社荏原製作所
发明人： ICHIKI KATSUNORI , MORITA TOMOYUKI , MIYA AKIKO
IPC分类号： G01N27/447 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide an apparatus capable of performing analysis having high reproducibility by a simple operation, concerning a microchip electrophoretic apparatus for separating double-stranded DNA according to a difference of a base sequence. SOLUTION: This apparatus uses a microchip 9 having a concentration gradient domain channel 27 on which a concentration gradient of a nucleic acid modifier is formed, a plurality of reservoirs 10 and a channel 26 for supplying two buffer solutions including a modifier on the upstream side in each different concentration, and a sample introduction part 28 for introducing a double-stranded nucleic acid sample on the downstream side. In the microchip 9, a high-voltage introduction terminal for applying a voltage to the buffer solution in the reservoir 10 is arranged. A packing for holding a solution on the position of the reservoir 10 is stored by an upper plate and a lower plate having a hole penetrating vertically. The apparatus has a structure wherein the high-voltage introduction terminal and a temperature control mechanism are integrated, and the temperature control mechanism has a contact with a part whose temperature is to be adjusted by using a plate spring, and the high-voltage introduction terminal is arranged in the packing. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：解决的问题：提供一种能够通过简单的操作进行具有高再现性的分析的装置，涉及根据碱基序列的差异分离双链DNA的微芯片电泳装置。解决方案：该装置使用具有浓度梯度域通道27的微芯片9，其上形成有核酸改性剂的浓度梯度，多个储存器10和通道26，用于将包含改性剂的两种缓冲溶液上游侧，以及用于在下游侧引入双链核酸样品的样品导入部28。在微芯片9中，设置有用于向储存器10中的缓冲溶液施加电压的高压引入端子。用于将溶液保持在储存器10的位置的填料通过具有垂直穿透的孔的上板和下板储存。该装置具有将高压引入端子和温度控制机构一体化的结构，并且温度控制机构通过使用板簧与要调节温度的部分接触，并且高压引入端子排列在包装中。版权所有（C）2011，JPO＆INPIT

40. 发明专利

JP2008099685A Method and apparatus for amplification and synthesis of nucleic acid with modifying agent 审中-公开
标题翻译：用改性剂对核酸进行放大和合成的方法和装置
公开(公告)号：JP2008099685A
公开(公告)日：2008-05-01
申请号：JP2007254209
申请日：2007-09-28
申请人： Ebara Corp , 株式会社荏原製作所
发明人： MORITA TOMOYUKI , MATSUMURA TAKASHI , MIYA AKIKO , NOMI MOTOHIKO , SHIMIZU SHUNSUKE
IPC分类号： C12N15/09 , C12M1/00
CPC分类号： C12P19/34
摘要： PROBLEM TO BE SOLVED: To provide a method for amplifying a nucleic acid, an apparatus for amplifying a nucleic acid, and a method and an apparatus for synthesizing a nucleic acid to be used in the amplification. SOLUTION: The nucleic acid amplifying method contains a step to modify a target nucleic acid in a region containing a modifying agent in an amount sufficient for modifying the nucleic acid, a step to hybridize a primer to the modified target nucleic acid, a step to modify a primer extension product by a manner same as the former step, a step to hybridize a primer to the modified product, and a step to extend the primer. COPYRIGHT: (C)2008,JPO&INPIT

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