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    • 3. 发明公开
    • HUMAN TRYPSINOGEN WITH REDUCED AUTOACTIVATION AND ITS USE IN AN IMMUNOASSAY
    • 美容美体麻醉师麻醉师减肥药AUTOSKTIVIERUNG UND DESSEN VERWENDUNG IN EINEM IMMUNSTST
    • EP3052623A1
    • 2016-08-10
    • EP14781104.6
    • 2014-09-25
    • Universität Leipzig
    • BÜTTNER, KarinPRASSE, AgnetaZÜCHNER, Thole
    • C12N9/76C12Q1/37G01N33/542G01N33/58
    • C12N9/6427C12Q1/37C12Y304/21004G01N33/542
    • The present invention relates to a polypeptide consisting of or comprising a variant of human trypsinogen-1, wherein the variant is characterized in that an amino acid residue selected from A16, A17, K64, K138, N139, L142 and L147 is replaced by an amino acid residue with a negatively charged side chain and/or H18 is replaced by a glutamine or asparagine residue and/or L107 is replaced by an amino acid residue with a positively charged side chain and/or N139 is replaced by an amino acid residue with an aromatic side chain, wherein the variant is cleavable into a polypeptide having a native-like enzymatic activity when compared to human trypsin-1. The present invention further relates to means and method for manufacturing the polypeptide as well as the use of the polypeptide, particularly in an immunoassay.
    • 本发明涉及由胰蛋白酶原-1变体组成或包含人胰蛋白酶原-1变体的多肽,其特征在于,选自A16,A17,K64,K138,N139,L142和L147的氨基酸残基被氨基酸 具有带负电荷侧链和/或H18的酸残基被谷氨酰胺或天冬酰胺残基替代,和/或L107被具有带正电荷侧链的氨基酸残基替代,和/或N139被氨基酸残基替代, 芳族侧链,其中当与人胰蛋白酶-1相比时,该变体可切割成具有天然样酶活性的多肽。 本发明还涉及用于制备多肽的方法和方法以及多肽的使用,特别是在免疫测定中。