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1. 发明公开

EP3270152A1 KIT FOR PREPARING SAMPLE FOR DETECTING MONOCLONAL ANTIBODY 审中-公开
标题翻译：制备样品用于检测单克隆抗体的试剂盒
公开(公告)号：EP3270152A1
公开(公告)日：2018-01-17
申请号：EP15884711.1
申请日：2015-12-18
申请人： Shimadzu Corporation
发明人： SHIMADA Takashi , IWAMOTO Noriko
IPC分类号： G01N30/72 , G01N1/10 , G01N27/62 , G01N30/00 , G01N30/06 , C12N11/02 , C12Q1/00 , C12Q1/37
CPC分类号： G01N33/6848 , C12N11/02 , C12Q1/00 , C12Q1/37 , G01N1/10 , G01N30/06 , G01N30/7233 , G01N33/577 , G01N2030/067 , G01N2333/901
摘要： A sample preparation kit related to the present invention provides a significantly versatile analytical technique that is not affected by the diversity of antibodies, difference in species, matrix and the like. For preparing a sample to be used for detection of a monoclonal antibody through high-performance liquid chromatography-mass spectrometry (LC-MS), the kit includes a porous body for immobilizing a monoclonal antibody to be detected; nanoparticles with an immobilized protease; a reaction vessel for selectively digesting the monoclonal antibody by bringing the porous body and nanoparticles into contact; a buffer to be introduced into the reaction vessel along with the nanoparticles and porous body so that a protease reaction is carried out; and a filtration membrane to remove the porous body and nanoparticles after the proteolysis so as to extract the reaction product and the buffer.
摘要翻译：与本发明有关的样品制备试剂盒提供了不受抗体多样性，物种差异，基质等影响的显着通用的分析技术。为了制备用于通过高效液相色谱 - 质谱（LC-MS）检测单克隆抗体的样品，该试剂盒包括用于固定待检测单克隆抗体的多孔体; 具有固定化蛋白酶的纳米颗粒; 通过使多孔体和纳米颗粒接触来选择性消化单克隆抗体的反应容器; 与纳米颗粒和多孔体一起引入反应容器中的缓冲液，从而进行蛋白酶反应; 和过滤膜以在蛋白水解之后去除多孔体和纳米颗粒以提取反应产物和缓冲液。

2. 发明公开

EP0854934A1 DETECTION OF ORGANIC COMPOUNDS THROUGH REGULATION OF ANTIBODY-CATALYZED REACTIONS 失效
标题翻译：检测有机化合物调控抗体催化反应作者：
公开(公告)号：EP0854934A1
公开(公告)日：1998-07-29
申请号：EP96930519.0
申请日：1996-08-16
申请人： Landry, Donald W.
发明人： Landry, Donald W.
IPC分类号： G01N33
CPC分类号： G01N33/5308 , G01N33/577 , G01N33/68 , G01N2333/901
摘要： The present invention provides a method for determining the presence of a target organic molecule in a sample which comprises either: (A) first, adding a substrate molecule to the sample, the substrate molecule being a conjugate of the target organic molecule and a reporter molecule; second, adding a catalytic monoclonal antidoby to the sample which: (1) recognizes and binds the target organic molecule when the target organic molecule is present in the sample and (2) recognizes and binds the substrate molecule when the target organic molecule is not present in the sample; and third detecting a change in the sample signifying the absence of the target organic molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of an organic molecule in the sample; or (B) first adding a substrate molecule to the sample, the substrate molecule being a conjugate of a molecule complementary to the target molecule and a reporter molecule; second adding a catalytic monoclonal antibody to the sample which: (1) recognizes and binds the target organic molecule when the target molecule is present in the sample and (2) recognizes and binds the substrate molecule only if the target molecule is present in the sample; and third detecting a change in the sample signifying the presence of the target molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of the target organic molecule in the sample.

3. 发明公开

EP0512905A2 Heterobifunctional antibodies possessing both catalytic and specific antigen binding properties and methods using them 失效
标题翻译：异型双抗体具有催化作用和抗原特异性结合的属性和它们的使用方法。
公开(公告)号：EP0512905A2
公开(公告)日：1992-11-11
申请号：EP92401242.0
申请日：1992-04-30
申请人： PASTEUR SANOFI DIAGNOSTICS
发明人： Newman, Karel , Schmidt, Jane
IPC分类号： C12P21/08 , C07K15/28 , C12P21/00 , G01N33/577 , C12Q1/30 , A61K39/395 , G01N33/53
CPC分类号： C12N9/0002 , A61K38/00 , C07K16/4283 , C07K16/44 , C07K16/468 , C12Q2334/10 , G01N2333/901
摘要： A novel and useful class of antibody molecules is described which possess the characteristics of an antigen-specific antibody and a catalytic antibody. Such a hybrid molecule may be assembled in vitro using biochemical means or using in vivo methods including cell hybridization, or recombinant DNA technologies. The product of the invention possesses both antigen target specificity as well as an intrinsic catalytic activity. Further said product may be used in any appropriate application including diagnostic test as a substitute for an enzyme-labelled antibody conjugate or as a therapeutic reagent whereby a part of the antibody molecule recognizes a unique cellular marker and the catalytic portion of antibody molecule has therepeutic activity.
摘要翻译：一种新颖的和有用的一类抗体分子，描述了具有抗原特异性抗体和催化抗体的特性。这样的杂合分子可在体外使用生物化学方法或使用体内方法，包括细胞杂交，或重组DNA技术进行组装。本发明的产物具有两个抗原的靶特异性以及对固有的催化活性。此外，所述产品可以以任何适当的应用程序被使用，包括诊断测试作为酶标记的抗体缀合物或作为一种治疗试剂由此抗体分子的一部分识别独特的细胞标记物和抗体分子的催化部分的替代品具有therepeutic活性，

4. 发明授权

EP0870014B9 ALDOLASE CATALYTIC ANTIBODY 失效
标题翻译：醛脱羧酶催化抗体
公开(公告)号：EP0870014B9
公开(公告)日：2007-10-10
申请号：EP96943772.2
申请日：1996-12-16
申请人： THE SCRIPPS RESEARCH INSTITUTE
发明人： BARBAS, Carlos, F., III , LERNER, Richard, A. , WAGNER, Juergen
IPC分类号： C12N9/00 , C12N5/12 , C12P7/26 , C12N9/88
CPC分类号： C12P7/26 , C12N9/0002 , C12N9/88 , G01N2333/901
摘要： Antibodies that catalyse the aldol reaction are generated by immunization with a reactive compound that covalently traps a Lysine (Lys) residue in the binding pocket of the antibody by formation of a stable vinylogous amide, i.e., a covalent antibody/hapten complex. The resultant catalytic antibodies employ a catalytic mechanism which mimics the catalytic mechanism employed by natural class I aldolase enzymes.
摘要翻译：通过用反应性化合物免疫产生催化醛醇反应的抗体，所述反应性化合物通过形成稳定的乙烯基酰胺即共价抗体/半抗原复合物，共价捕获抗体结合口袋中的赖氨酸（Lys）残基。所得到的催化抗体使用模拟天然I类醛缩酶使用的催化机制的催化机制。

5. 发明授权

EP0870014B1 ALDOLASE CATALYTIC ANTIBODY 失效
标题翻译：催化抗体醛缩酶
公开(公告)号：EP0870014B1
公开(公告)日：2007-02-21
申请号：EP96943772.2
申请日：1996-12-16
申请人： THE SCRIPPS RESEARCH INSTITUTE
发明人： BARBAS, Carlos, F., III , LERNER, Richard, A. , WAGNER, Juergen
IPC分类号： C12N9/00 , C12N5/12 , C12P7/26 , C12N9/88
CPC分类号： C12P7/26 , C12N9/0002 , C12N9/88 , G01N2333/901
摘要： Antibodies that catalyse the aldol reaction are generated by immunization with a reactive compound that covalently traps a Lysine (Lys) residue in the binding pocket of the antibody by formation of a stable vinylogous amide, i.e., a covalent antibody/hapten complex. The resultant catalytic antibodies employ a catalytic mechanism which mimics the catalytic mechanism employed by natural class I aldolase enzymes.

6. 发明公开

EP1390478A1 COMPOSES CAPABLES DE MODULER L'ACTIVITE ET DE STIMULER LA PRODUCTION D'UN ANTICORPS CATALYTIQUE 审中-公开
标题翻译：催化抗体MODULATE其生产的活性化合物刺激
公开(公告)号：EP1390478A1
公开(公告)日：2004-02-25
申请号：EP02732880.6
申请日：2002-05-22
申请人： Gradient
发明人： FRIBOULET, Alain , AVALLE-BIHAN, Bérangère , DEBAT, Hélène , THOMAS, Daniel
IPC分类号： C12N9/00 , A61K38/19 , A61K38/16
CPC分类号： C12N9/0002 , A61K38/06 , A61K38/191 , G01N33/6854 , G01N33/6878 , G01N2333/901 , G01N2500/00
摘要： The invention concerns novel compounds capable of modulating the activity and stimulating the production of catalytic antibodies. More particularly, the invention concerns a compound capable of increasing the activity of an antibody characterised in that it has specific affinity for the catalytic site of said antibody, and it is not immunogenic. Such compounds are useful in particular for therapeutic treatment or prevention of a pathology related to an enzymatic deficiency, to stimulate in vivo hydrolysis of xenobiotics, drugs, medicines or any other molecule potentially toxic for the organism or for preventing or inhibiting allergic reactions.

7. 发明公开

EP2494064A1 A PROXIMITY LIGATION ASSAY INVOLVING GENERATION OF CATALYTIC ACTIVITY 审中-公开
标题翻译：随着生产的催化活性邻位连接检定
公开(公告)号：EP2494064A1
公开(公告)日：2012-09-05
申请号：EP10787529.6
申请日：2010-10-26
申请人： Axis-Shield Diagnostics Limited
发明人： SMITH, Adrian , DEHAL, Prabhjyot , ØRNING, Lars Gøte Ingemar
IPC分类号： C12Q1/00 , G01N33/542 , C12Q1/68
CPC分类号： G01N33/542 , C12Q1/00 , G01N2333/901 , G01N2333/9015 , G01N2458/10
摘要： A homogenous assay comprising the steps: 1) contacting at least two molecules each comprising a recognition component and a partial message with a sample wherein the contact of the recognition components with the sample results in the partial messages forming a continuous message; 2) amplifying the continuous message to form a catalytic entity which either directly or indirectly generates catalytic activity; 3) providing a substrate which allows detection of a recognition event between the at least two molecules and the sample via the catalytic activity.

8. 发明公开

EP0688431A4 METHOD FOR SCREENING CATALYTIC NON-ENZYME POLYPEPTIDES AND PROTEINS 失效
标题翻译：筛选方法，催化非酶多肽和蛋白质
公开(公告)号：EP0688431A4
公开(公告)日：1998-03-18
申请号：EP94909451
申请日：1994-01-12
申请人： YEDA RES & DEV , YISSUM RES DEV CO
发明人： ESHHAR ZELIG , GREEN BERNARD S , TAWFIK DAN S
IPC分类号： G01N33/53 , C12N15/02 , C12N15/10 , C12P21/08 , C12R1/91 , G01N33/543 , G01N33/573 , G01N33/68
CPC分类号： C12N15/1034 , G01N33/573 , G01N33/6854 , G01N2333/901
摘要： A method for screening or detection of a non-enzyme catalytic polypeptide or protein for the conversion of a substrate S to a product P is provided, in which a preparation containing the potential catalyst is contacted with the substrate S immobilized to a support and the immobilized product P obtained is detected, preferably by immunoassay. The method is preferably used for the screening of hybridoma supernatants for catalytic monoclonal antibodies that catalyze acyl transfer reactions, e.g., hydrolysis or aminolysis, condensation reactions and resolution of enantiomers.

9. 发明授权

EP0512905B1 Heterobifunctional antibodies possessing both catalytic and specific antigen binding properties and methods using them 失效
标题翻译：与两个催化性质以及特定的抗原结合特性和它们的使用方法的异双抗体
公开(公告)号：EP0512905B1
公开(公告)日：1997-10-29
申请号：EP92401242.0
申请日：1992-04-30
申请人： PASTEUR SANOFI DIAGNOSTICS
发明人： Newman, Karel , Schmidt, Jane
IPC分类号： C12P21/08 , C07K16/00 , C12P21/00 , G01N33/577 , C12Q1/30 , A61K39/395 , G01N33/53
CPC分类号： C12N9/0002 , A61K38/00 , C07K16/4283 , C07K16/44 , C07K16/468 , C12Q2334/10 , G01N2333/901

10. 发明公开

EP0870014A4 ALDOLASE CATALYTIC ANTIBODY 失效
标题翻译：催化抗体醛缩酶
公开(公告)号：EP0870014A4
公开(公告)日：2002-03-13
申请号：EP96943772
申请日：1996-12-16
申请人： SCRIPPS RESEARCH INST
发明人： BARBAS CARLOS F III , LERNER RICHARD A , WAGNER JUERGEN
IPC分类号： C07C45/45 , C07C231/12 , C07C233/33 , C07K16/44 , C12N5/10 , C12N5/18 , C12N9/00 , C12N9/88 , C12N15/06 , C12P7/26 , C12P13/00 , C12P21/08 , C12N5/12
CPC分类号： C12P7/26 , C12N9/0002 , C12N9/88 , G01N2333/901

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