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1. 发明公开

EP0854934A4 DETECTION OF ORGANIC COMPOUNDS THROUGH REGULATION OF ANTIBODY-CATALYZED REACTIONS 失效
标题翻译：检测有机化合物调控抗体催化反应作者：
公开(公告)号：EP0854934A4
公开(公告)日：2000-09-06
申请号：EP96930519
申请日：1996-08-16
申请人： LANDRY DONALD W
发明人： LANDRY DONALD W
IPC分类号： G01N33/53 , G01N33/577 , G01N33/68 , C12Q1/25 , C12Q1/44
CPC分类号： G01N33/5308 , G01N33/577 , G01N33/68 , G01N2333/901
摘要： The present invention provides a method for determining the presence of a target organic molecule in a sample which comprises either (A): first, adding a substrate molecule to the sample, the substrate molecule being a conjugate of the target organic molecule and a reporter molecule; second, adding a catalytic monoclonal antibody to the sample which (1) recognizes and binds the target organic molecule when the target organic molecule is present in the sample and (2) recognizes and binds the substrate molecule when the target organic molecule is not present in the sample; and third detecting a change in the sample signifying the absence of the target organic molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of an organic molecule in the sample; or (B): first adding a substrate molecule to the sample, the substrate molecule being a conjugate of a molecule complementary to the target molecule and a reporter molecule; second adding a catalytic monoclonal antibody to the sample which (1) recognizes and binds the target organic molecule when the target molecule is present in the sample and (2) recognizes and binds the substrate molecule only if the target molecule is present in the sample; and third detecting a change in the sample signifying the presence of the target molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of the target organic molecule in the sample.

2. 发明公开

EP0854934A1 DETECTION OF ORGANIC COMPOUNDS THROUGH REGULATION OF ANTIBODY-CATALYZED REACTIONS 失效
标题翻译：检测有机化合物调控抗体催化反应作者：
公开(公告)号：EP0854934A1
公开(公告)日：1998-07-29
申请号：EP96930519.0
申请日：1996-08-16
申请人： Landry, Donald W.
发明人： Landry, Donald W.
IPC分类号： G01N33
CPC分类号： G01N33/5308 , G01N33/577 , G01N33/68 , G01N2333/901
摘要： The present invention provides a method for determining the presence of a target organic molecule in a sample which comprises either: (A) first, adding a substrate molecule to the sample, the substrate molecule being a conjugate of the target organic molecule and a reporter molecule; second, adding a catalytic monoclonal antidoby to the sample which: (1) recognizes and binds the target organic molecule when the target organic molecule is present in the sample and (2) recognizes and binds the substrate molecule when the target organic molecule is not present in the sample; and third detecting a change in the sample signifying the absence of the target organic molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of an organic molecule in the sample; or (B) first adding a substrate molecule to the sample, the substrate molecule being a conjugate of a molecule complementary to the target molecule and a reporter molecule; second adding a catalytic monoclonal antibody to the sample which: (1) recognizes and binds the target organic molecule when the target molecule is present in the sample and (2) recognizes and binds the substrate molecule only if the target molecule is present in the sample; and third detecting a change in the sample signifying the presence of the target molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of the target organic molecule in the sample.

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