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1. 发明公开

EP1021717A1 METHOD OF SORTING BIRDS IN OVO 失效
标题翻译：方法在OVO鸟类性别鉴定
公开(公告)号：EP1021717A1
公开(公告)日：2000-07-26
申请号：EP97912688.5
申请日：1997-10-03
申请人： EMBREX INC.
发明人： PHELPS, Patricia, V.
IPC分类号： G01N33/50 , G01N33/74 , A01K67/00
CPC分类号： G01N33/5088 , A01K43/00 , G01N33/50 , G01N33/743 , G01N2333/465 , Y10S530/827 , Y10S530/852 , Y10S530/853 , Y10T436/10 , Y10T436/143333
摘要： A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

2. 发明授权

EP0759065B1 Process and device for evaluating fertility of spermatozoa samples 失效
标题翻译：用于确定Spermatozoenproben的Fruchtarbarkeit方法和装置
公开(公告)号：EP0759065B1
公开(公告)日：2002-07-03
申请号：EP95917680.1
申请日：1995-04-26
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： HAMMERSTEDT, Roy H. , BARBATO, Guy F. , CRAMER, Palmer G.
IPC分类号： C12M3/04 , C12M3/10 , C12Q1/02 , C12Q1/24 , G01N33/68 , G01N33/566
CPC分类号： G01N33/689 , G01N33/5005 , G01N33/5091 , G01N2333/465 , G01N2800/367 , Y10S435/806
摘要： A method for testing the potential fertility of spermatozoa in a sample by contacting an aliquot of the sperm sample onto a protein extracted from native vitelline membranes removed from chicken or turkey eggs to enable quantification of the number of sperm which bind to the protein. Typically, the vitelline membrane protein is coated onto a solid substrate and the protein extract is first prepared by dissecting vitelline membranes (lamina perivitellina plus lamina extravitellina) from either a group of chicken eggs or a group of turkey eggs, rinsing the membranes free of albumin and yolk, and subdividing the membranes into small particles. The resulting protein is heat solubilized, purified with centrifugation and concentrated to yield the protein extract in solution. The extracted protein then is used as a protein substrate for spermatozoa binding, with sequential contacting of the protein extract with the spermatozoa and measuring the amount of sperm-egg binding that takes place. Intact egg membranes may alternatively be used to bind sperm. Correlation between sperm-egg binding and fertility of the spermatozoa in the sample is direct and linear.

3. 发明公开

EP0966478A4 NOVEL HEDGEHOG-DERIVED POLYPEPTIDES 失效
标题翻译： NEW来自IGEL的多肽
公开(公告)号：EP0966478A4
公开(公告)日：2002-08-21
申请号：EP97910705
申请日：1997-10-07
申请人： UNIV JOHNS HOPKINS MED
发明人： BEACHY PHILIP A , PORTER JEFFREY A
IPC分类号： G01N33/53 , A61K38/00 , C07K14/435 , C07K14/46 , C07K14/47 , C12N15/09 , G01N33/50 , G01N33/68 , C07K1/00
CPC分类号： G01N33/5058 , A61K38/00 , C07K14/43581 , C07K14/46 , C07K14/47 , C07K2319/21 , C07K2319/23 , C07K2319/50 , G01N33/5008 , G01N33/5011 , G01N33/502 , G01N33/5091 , G01N33/68 , G01N2333/43573 , G01N2333/4603 , G01N2333/4606 , G01N2333/465 , G01N2333/47 , G01N2500/10
摘要： The present invention provides two novel polypeptides, referred to as the "N" and "C" fragments of hedgehog, or N-terminal and C-terminal fragments, respectively, which are derived after specific cleavage at a G &darr& CF site recognized by the autoproteolytic domain in the native protein. Also included are sterol-modified hedgehog polypeptides and functional fragments thereof. Methods of identifying compositions which affect hedgehog activity based on inhibition of cholesterol modification of hedgehog protein are described.

4. 发明公开

EP0759065A1 PROCESS FOR EVALUATING FERTILITY OF SPERMATOZOA SAMPLES 失效
标题翻译：用于确定精子样本的生育力
公开(公告)号：EP0759065A1
公开(公告)日：1997-02-26
申请号：EP95917680.0
申请日：1995-04-26
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： HAMMERSTEDT, Roy H. , BARBATO, Guy F. , CRAMER, Palmer G.
IPC分类号： A61D19 , C12M1 , C12M3 , G01N33
CPC分类号： G01N33/689 , G01N33/5005 , G01N33/5091 , G01N2333/465 , G01N2800/367 , Y10S435/806
摘要： A method for testing the potential fertility of spermatozoa in a sample by contacting an aliquot of the sperm sample onto a protein extracted from native vitelline membranes removed from chicken or turkey eggs to enable quantification of the number of sperm which bind to the protein. Typically, the vitelline membrane protein is coated onto a solid substrate and the protein extract is first prepared by dissecting vitelline membranes (lamina perivitellina plus lamina extravitellina) from either a group of chicken eggs or a group of turkey eggs, rinsing the membranes free of albumin and yolk, and subdividing the membranes into small particles. The resulting protein is heat solubilized, purified with centrifugation and concentrated to yield the protein extract in solution. The extracted protein then is used as a protein substrate for spermatozoa binding, with sequential contacting of the protein extract with the spermatozoa and measuring the amount of sperm-egg binding that takes place. Intact egg membranes may alternatively be used to bind sperm. Correlation between sperm-egg binding and fertility of the spermatozoa in the sample is direct and linear.

5. 发明公开

EP2124056A1 IMMUNOLOGICAL DETECTION METHOD USING AVIAN ANTIBODY 审中-公开
标题翻译：免疫学杂志VERVENSION VERWENDUNG VONVOGELANTIKÖRPER
公开(公告)号：EP2124056A1
公开(公告)日：2009-11-25
申请号：EP07860356.0
申请日：2007-12-27
申请人： Japan Science and Technology Agency , Hiroshima University , WAKUNAGA PHARMACEUTICAL CO., LTD.
发明人： MATSUDA, Haruo , KANEZAWA, Fujiko , KUBO, Hiroyuki , NISHIMICHI, Norihisa , AOSASA, Masayoshi
IPC分类号： G01N33/531
CPC分类号： G01N33/6857 , G01N2333/465
摘要： An object of the present invention is to provide an immunological detection method using an avian antibody, comprising suppressing a non-specific reaction caused by using an avian antibody. According to the present invention, there is provided an immunological detection method comprising detecting a target substance in a sample from a mammal using an avian antibody, characterized in that a non-specific reaction induced by the use of the avian antibody is suppressed.
摘要翻译：本发明的目的是提供一种使用禽类抗体的免疫学检测方法，包括抑制由使用禽抗体引起的非特异性反应。根据本发明，提供了一种免疫学检测方法，其包括使用禽类抗体检测来自哺乳动物的样品中的目标物质，其特征在于抑制由使用禽抗体诱导的非特异性反应。

6. 发明公开

EP0856055A1 STREPTAVIDIN MUTANTS 失效
标题翻译： STREPTAVIDIN突变体
公开(公告)号：EP0856055A1
公开(公告)日：1998-08-05
申请号：EP96916428.0
申请日：1996-04-10
申请人： TRUSTEES OF BOSTON UNIVERSITY
发明人： SANO, Takeshi , CANTOR, Charles, R. , VAJDA, Sandor , REZNIK, Gabriel, O. , SMITH, Cassandra, L. , PANDORI, Mark, W.
IPC分类号： C07K14 , C12N1 , C12N15 , G01N33 , A61K38
CPC分类号： G01N33/538 , A61K38/00 , C07K14/36 , G01N2333/465 , Y10S530/808 , Y10S530/81
摘要： The present invention relates to streptavidin proteins and peptides having altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.
摘要翻译：本发明涉及链霉抗生物素蛋白和肽，其具有改变的物理性质，例如稳定性提高或对结合生物素的亲和力增加或降低。本发明还涉及使用链霉抗生物素蛋白或肽检测，鉴定，分离和分离靶标的方法。具有增加的或降低的亲和力的链霉抗生物素蛋白允许使用链霉抗生物素蛋白 - 生物素偶联系统用于检测和分离系统，其中有必要除去一个或另一个结合伴侣。这样的系统可用于功能性蛋白质和活细胞的纯化。本发明还涉及编码这些链霉抗生物素蛋白和肽的核酸，以及包含这些核酸的重组细胞，例如细菌，酵母和哺乳动物细胞。

7. 发明公开

EP0759065A4 PROCESS FOR EVALUATING FERTILITY OF SPERMATOZOA SAMPLES 失效
标题翻译：用于确定精子样本的生育力
公开(公告)号：EP0759065A4
公开(公告)日：1997-05-14
申请号：EP95917680
申请日：1995-04-26
申请人： PENN STATE RES FOUND
发明人： HAMMERSTEDT ROY H , BARBATO GUY F , CRAMER PALMER G
IPC分类号： A61D19/00 , C12M1/20 , C12M3/00 , C12M3/04 , G01N33/50 , G01N33/53 , G01N33/68 , C12M3/10 , C12Q1/02 , C12Q1/24
CPC分类号： G01N33/689 , G01N33/5005 , G01N33/5091 , G01N2333/465 , G01N2800/367 , Y10S435/806
摘要： A method for testing the potential fertility of spermatozoa in a sample by contacting an aliquot of the sperm sample onto a protein extracted from native vitelline membranes removed from chicken or turkey eggs to enable quantification of the number of sperm which bind to the protein. Typically, the vitelline membrane protein is coated onto a solid substrate and the protein extract is first prepared by dissecting vitelline membranes (lamina perivitellina plus lamina extravitellina) from either a group of chicken eggs or a group of turkey eggs, rinsing the membranes free of albumin and yolk, and subdividing the membranes into small particles. The resulting protein is heat solubilized, purified with centrifugation and concentrated to yield the protein extract in solution. The extracted protein then is used as a protein substrate for spermatozoa binding, with sequential contacting of the protein extract with the spermatozoa and measuring the amount of sperm-egg binding that takes place. Intact egg membranes may alternatively be used to bind sperm. Correlation between sperm-egg binding and fertility of the spermatozoa in the sample is direct and linear.

8. 发明公开

EP3305809A1 QUAIL EGG ALLERGY ANTIGEN 审中-公开
标题翻译：鹌鹑蛋过敏抗原
公开(公告)号：EP3305809A1
公开(公告)日：2018-04-11
申请号：EP16800080.0
申请日：2016-05-25
申请人： Hoyu Co., Ltd.
发明人： MATSUNAGA, Kayoko , YAGAMI, Akiko , KONDO, Yasuto , HARA, Kazuhiro , NAKAMURA, Masashi , AOKI, Yuji
IPC分类号： C07K14/465 , A23L15/00 , A61K39/35 , A61P37/08 , G01N33/53
CPC分类号： A61K39/35 , A23L15/00 , A23V2002/00 , C07K14/465 , C07K16/18 , G01N33/53 , G01N2333/465
摘要： The present invention provides novel antigens of an allergy to a quail egg, methods and kits for diagnosing an allergy to a quail egg, pharmaceutical compositions comprising such an antigen, and quail eggs or processed products of quail egg which are devoid of such an antigen.
摘要翻译：本发明提供了对鹌鹑卵过敏的新型抗原，诊断对鹌鹑卵过敏的方法和试剂盒，包含这种抗原的药物组合物，以及不含这种抗原的鹌鹑蛋或鹌鹑蛋加工产品。

9. 发明公开

EP2124056A4 IMMUNOLOGICAL DETECTION METHOD USING AVIAN ANTIBODY 审中-公开
标题翻译：免疫学检测方法，使用VOGEL ANTIBODY
公开(公告)号：EP2124056A4
公开(公告)日：2010-06-02
申请号：EP07860356
申请日：2007-12-27
申请人： JAPAN SCIENCE & TECH AGENCY , UNIV HIROSHIMA , WAKUNAGA PHARMA CO LTD
发明人： MATSUDA HARUO , KANEZAWA FUJIKO , KUBO HIROYUKI , NISHIMICHI NORIHISA , AOSASA MASAYOSHI
IPC分类号： G01N33/531 , G01N33/543
CPC分类号： G01N33/6857 , G01N2333/465

10. 发明授权

EP1021717B1 METHOD OF SORTING BIRDS IN OVO 失效
标题翻译：方法在OVO鸟类性别鉴定
公开(公告)号：EP1021717B1
公开(公告)日：2002-04-10
申请号：EP97912688.5
申请日：1997-10-03
申请人： EMBREX INC.
发明人： PHELPS, Patricia, V.
IPC分类号： G01N33/50 , G01N33/74 , A01K67/00
CPC分类号： G01N33/5088 , A01K43/00 , G01N33/50 , G01N33/743 , G01N2333/465 , Y10S530/827 , Y10S530/852 , Y10S530/853 , Y10T436/10 , Y10T436/143333
摘要： A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

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