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1. 发明公开

EP3056901A1 Verfahren zum Nachweis von Proteinen mit Hilfe von Aptameren 审中-公开
公开(公告)号：EP3056901A1
公开(公告)日：2016-08-17
申请号：EP15075007.3
申请日：2015-02-13
申请人： Technische Universität Berlin
发明人： Kurreck, Jens , Wagner, Anke , Röhrs, Viola
IPC分类号： G01N33/53 , G01N33/68
CPC分类号： G01N33/573 , G01N33/5306 , G01N33/68 , G01N33/6803 , G01N2333/36 , G01N2333/974
摘要： Die vorliegende Erfindung beschreibt ein Verfahren zum in vitro Nachweis eines ersten Proteins in einer Probe umfassend die Schritte a) Auftrennen der Probe mittels eines nativen Trennverfahrens, b) Transfer der aufgetrennten Probe auf eine Membran, c) in Kontakt bringen der Membran mit einem Aptamer, das spezifisch an das erste Protein bindet, und d) Nachweis des ersten Proteins mittels Detektion des Aptamers, das an das erste Protein gebunden vorliegt.
摘要翻译：本发明描述了一种体外检测样品中第一种蛋白质的方法，包括以下步骤：a）通过天然分离方法分离样品; b）将分离的样品转移到膜上; c）使膜与与第一蛋白特异性结合的适体接触; 和d）通过检测与第一种蛋白质结合的适体来检测第一种蛋白质。

2. 发明公开

EP2960243A1 BIOTIN ALTERANT, STREPTAVIDIN MUTANT, AND USES THEREOF 有权
标题翻译：生物素修饰剂，Streptovidin突变体及其用途
公开(公告)号：EP2960243A1
公开(公告)日：2015-12-30
申请号：EP14753647.8
申请日：2014-02-18
申请人： Savid Therapeutics Inc.
发明人： SUGIYAMA, Akira , DOI, Hirofumi , KODAMA, Tatsuhiko , INOUE, Tsuyoshi , MIZOHATA, Eiichi , KAWATO, Tatsuya , MESHIZUKA, Tomohiro , KANAI, Motomu , SHIMIZU, Yohei , YAMAMOTO, Tomohiro
IPC分类号： C07D495/04 , C07D497/04 , C07D498/04 , C07K14/36 , C12N15/09 , G01N33/53
CPC分类号： C07K14/36 , C07D495/04 , C07D497/04 , C07D498/04 , G01N33/54306 , G01N2333/36
摘要： It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.
摘要翻译：本发明的一个目的是提供对天然生物素具有降低的亲和力的突变抗生蛋白链菌素，并且还提供对天然生物素具有降低的亲和力的对突变抗生蛋白链菌素具有高亲和力的修饰生物素。根据本发明，提供了用于治疗或诊断的试剂盒，其包括：（a）对天然生物素或生物胞素具有降低亲和力的突变链霉抗生物素蛋白; 和对上述突变链霉抗生物素蛋白具有高亲和力的修饰生物素。

3. 发明公开

EP2184356A4 METHOD OF BINDING PROTEIN TO SUPPORT USING TAMAVIDIN 审中-公开
标题翻译：方法用于结合蛋白进行车辆提供的tamavidin
公开(公告)号：EP2184356A4
公开(公告)日：2012-02-22
申请号：EP08828874
申请日：2008-08-28
申请人： JAPAN TOBACCO INC
发明人： TAKAKURA YOSHIMITSU , ICHIKAWA MASAKO , USAMI SATORU , YAMAMOTO TAKESHI , TSUKAMOTO HIROSHI , KAJIWARA HITOMI , OKA NAOMI
IPC分类号： C12N15/09 , C07K14/375 , C07K17/00 , C07K17/06 , C07K19/00
CPC分类号： C07K17/06 , C07K14/375 , C07K2319/20 , G01N2333/36

4. 发明公开

EP1865059A1 A novel protein, a gene encoding therefor and a method of using the same 有权
标题翻译： Neues Protein，dafürkodierendes Gen und Verwendungsverfahrendafür
公开(公告)号：EP1865059A1
公开(公告)日：2007-12-12
申请号：EP07101631.5
申请日：2002-03-12
申请人： Japan Tobacco, Inc.
发明人： Takakura, Yoshimitsu
IPC分类号： C12N15/09 , A01N65/00 , C07K14/375 , C12N5/10 , C12P21/02 , C12N15/82
CPC分类号： A01N63/04 , C07K14/375 , C12N15/8282 , G01N2333/36
摘要： An object of the present invention is to search and identify novel biotin-binding and antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.
摘要翻译：本发明的目的是搜索和鉴定能够抑制植物病原微生物生长的新型生物素结合和抗真菌蛋白质，其包括稻瘟病菌和丝核丝杆菌，其以相对低的浓度引起两种主要的水稻病害，并进一步克隆用于说蛋白质。本发明提供了一种抗真菌蛋白质，其可以使用来自侧耳属聚宝球蛋白的水提取物从硫酸铵沉淀中沉淀的级分获得，其中所述蛋白质具有至少抗稻瘟病的抗真菌活性，并且表现出存在具有通过SDS-PAGE法测定分子量约15kDa; 编码所述蛋白质的基因及其用途。

5. 发明公开

EP1402264A1 ASSAY FOR BETA CELL TOXIC MACROLIDES 审中-公开
标题翻译：测试β细胞毒性大环内酯类
公开(公告)号：EP1402264A1
公开(公告)日：2004-03-31
申请号：EP02773998.6
申请日：2002-05-22
申请人： MONASH UNIVERSITY , International Diabetes Institute
发明人： MYERS, Mark A.,Dept. of Biochem. & Molecular Bio. , MACKAY, Ian R.,Dept. of Biochem. & Molecular Bio. , ZIMMET, Paul Z.,International Diabetes Institute
IPC分类号： G01N33/564 , C12Q1/68 , A61P3/00 , A61K39/395 , A61K49/10 , A61K49/12
CPC分类号： C12Q1/6895 , A61K49/0004 , G01N33/56911 , G01N2333/36
摘要： The present invention relates to a method of assessing the propensity of a plant, if consumed, to contribute to the onset and/or progression of diabetes in a mammal and, more particularly, to a method of assessing the propensity of a tuberous vegetable, if consumed, to contribute to the onset and/or progression of diabetes in a mammal. The method of the present invention is useful, inter alia, for identifying mammals at risk of developing diabetes based on dietary intake. The present invention is further directed to methods of assessing the risk status of an individual for development of diabetes. The present invention still further provides methods for the prophylactic and/or therapeutic treatment of diabetes.

6. 发明公开

EP1325336A2 REAGENTS, METHOD AND KIT FOR DETECTING PHOSPHINOTHRICIN-N-ACETYLTRANSFERASE PROTEIN 审中-公开
标题翻译：试剂，方法和试剂盒TO膦N-乙酰转移酶蛋白来检测
公开(公告)号：EP1325336A2
公开(公告)日：2003-07-09
申请号：EP01979449.4
申请日：2001-09-28
申请人： STRATEGIC DIAGNOSTICS INDUSTRIES, INC.
发明人： ONISK, Dale, V. , STAVE, James, W. , MCQUILLIN, Alan, B.
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C07K14/36 , C07K16/40 , C12N9/1029 , C12N15/8274 , G01N2333/36 , G01N2333/91051
摘要： A method, kit and reagents for the detection of (phosphinothricin-N-acetyltransferase) PAT proteins in a sample, particularly a genetically modified agricultural crop sample. The proteins to be detected are one or more PAT enzyme proteins from various species of Streptomyces, including S. hygroscopicus and S. viridochromogenes. In particular, the PAT proteins are detected in genetically modified plants containing a gene, such as the pat or bar gene, that renders the plant resistant to the herbicide glufosinate. The reagents are antigenic peptides having common epitope of the PAT proteins from two or more Streptomyces species and antibodies immunoreactive with these proteins, particularly those expressed by the bar and pat genes. The peptides are isolated or synthesized and administered to animals to produce anti-PAT monoclonal and polyclonal antibodies having high sensitivity for PAT proteins. The antibodies are useful in immunoassay methods for the detection of genetically modified organisms engineered to include a PAT gene.

7. 发明授权

EP0419567B1 PROCESS FOR INDUCING CYTOCHROME P-450 ENZYMES IN STREPTOMYCES-BACTERIA AND DETERMINING THE MUTAGENICITY OF CHEMICALS 失效
标题翻译：在细菌细菌中诱导细胞色素P-450酶的过程以及测定化学品的致突变性
公开(公告)号：EP0419567B1
公开(公告)日：1994-03-23
申请号：EP89907571.7
申请日：1989-06-15
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： BUCHHOLZ, Steven Edward , OMER, Charles, Anthony , VIITANEN, Paul, Veikko
IPC分类号： C12N15/76 , C12Q1/02 , C12Q1/18 , C12R1/465
CPC分类号： C12N9/0077 , C12N15/76 , C12Q1/025 , C12Q1/10 , C12Q1/26 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y10S435/886 , Y10S435/897
摘要： A process for inducing cytochrome P-450 enzyme production in bacteria of the genus Streptomyces using inducers such as soybean flour, genistein or genistin is described. Uses for the cytochrome P-450 enzymes produced are also discussed as is a process for using genetically engineered Streptomyces to determine the mutagenicity of chemicals.
摘要翻译：描述了使用诱导剂如大豆粉，染料木黄酮或染料木黄酮在链霉菌属细菌中诱导细胞色素P-450酶生产的方法。还讨论了产生的细胞色素P-450酶的用途，以及使用基因工程链霉菌来确定化学品的致突变性的过程。

8. 发明授权

EP0273771B1 Mutagenic evaluation method 失效
标题翻译： MUTAGENIC评估方法
公开(公告)号：EP0273771B1
公开(公告)日：1993-09-22
申请号：EP87311520.8
申请日：1987-12-30
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： Sariaslani, Fateme Sima
IPC分类号： C12Q1/02 , C12Q1/18
CPC分类号： C12Q1/26 , C12Q1/025 , C12Q1/10 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y02A50/451
摘要： A method for evaluating the potential mutagenic activity of a substance which comprises: (a) culturing cytochrome P-450 induced bacteria from the genus Streptomyces in a culture medium comprising the substance to be evaluated; (b) incubating an amino acid requiring mutant strain of Salmonella typhimurium in the presence of a sample of the bacterial culture from (a); and (c) determining the number of resulting amino acid sufficient revertant colonies. a

9. 发明授权

EP0151783B1 Method for exposing bacterial antigen and improved diagnostic assay using same 失效
标题翻译：用于暴露细菌抗原的方法和使用其改进的诊断测定法
公开(公告)号：EP0151783B1
公开(公告)日：1991-06-05
申请号：EP84116001.3
申请日：1984-12-20
申请人： ABBOTT LABORATORIES
发明人： Krause-Hooyman, Laurel
IPC分类号： G01N33/569 , C12Q1/37
CPC分类号： C12Q1/37 , G01N33/56944 , G01N2333/315 , G01N2333/36 , Y10S435/961

10. 发明公开

EP0419567A1 PROCESS FOR INDUCING CYTOCHROME P-450 ENZYMES IN $i(STREPTOMYCES) BACTERIA AND DETERMINING THE MUTAGENICITY OF CHEMICALS 失效
标题翻译：诱导细胞色素P-450酶（链霉菌）细菌和确定化学品致突变性的方法
公开(公告)号：EP0419567A1
公开(公告)日：1991-04-03
申请号：EP89907571.0
申请日：1989-06-15
申请人： E.I. DU PONT DE NEMOURS AND COMPANY
发明人： BUCHHOLZ, Steven, Edward , OMER, Charles, Anthony , VIITANEN, Paul, Veikko
IPC分类号： C12N15 , C12N1 , C12N9 , C12Q1 , C12R1
CPC分类号： C12N9/0077 , C12N15/76 , C12Q1/025 , C12Q1/10 , C12Q1/26 , G01N2333/255 , G01N2333/36 , G01N2333/795 , G01N2333/80 , G01N2333/902 , G01N2333/90245 , Y10S435/886 , Y10S435/897
摘要： Le procédé décrit, qui permet de provoquer la production d'enzymes de cytochrome P-450 dans des bactéries du gène Streptomyces, utilise des agents incitateurs tels que de la farine de soya, de la génistéine or de la génistine. Les emplois des enzymes de cytochrome P-450 ainsi produites et un procédé d'utilisation de Streptomyces traités par génie génétique pour déterminer la mutagénicité de produits chimiques sont également décrits.
摘要翻译：所描述的方法使得诱导链霉菌基因细菌中细胞色素P-450酶的产生成为可能，使用诱导剂如大豆粉，染料木黄酮或染料木苷。还公开了使用如此产生的细胞色素P-450酶和使用基因工程链霉菌来测定化学品的致突变性的方法。

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