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    • 2. 发明公开
    • Device for use in the detection of binding affinities
    • Vorrichtung zur Verwendung bei derBindeaffinitätserkennung
    • EP2741074A1
    • 2014-06-11
    • EP12195532.2
    • 2012-12-04
    • F. Hoffmann-La Roche AG
    • The designation of the inventor has not yet been filed
    • G01N21/77
    • G01N21/75G01N21/7703G01N21/774G01N2021/752G01N2021/7776G01N2021/7793G01N2201/0438
    • A device for use in the detection of binding affinities, the device comprising a planar waveguide (2) arranged on a substrate (3), and further comprising an optical coupler (41) having a predetermined length for coupling coherent light (1) of a predetermined wavelength into the planar waveguide (2) such that a parallel beam of coherent light propagates through the planar waveguide (2) with an evanescent field (11) of the coherent light propagating along an outer surface (21) of the planar waveguide (2). The outer surface (21) of the planar waveguide (2) comprises binding sites thereon capable of binding target samples to the binding sites such that light of the evanescent field (11) is diffracted by target samples bound to the binding sites. The binding sites are arranged along a plurality of predetermined straight lines (7) running parallel to one another with a constant distance between adjacent straight lines. The predetermined straight lines (7) are arranged at an angle relative to the direction of propagation of the evanescent field (11) such that the coherent light (12) diffracted by the target samples bound to the binding sites impinges under a diffraction angle relative to the straight lines onto a further optical coupler (8) arranged in a portion (10) of the planar waveguide (2) outside the beam of coherent light propagating through the planar waveguide. The further optical coupler (8) couples the diffracted coherent light (13) out of the planar waveguide (2) such as to interfere at a predetermined detection location (9) with a difference in optical path length which is an integer multiple of the predetermined wavelength.
    • 一种用于检测结合亲和力的装置,该装置包括布置在基板(3)上的平面波导(2),并且还包括具有预定长度的光耦合器(41),用于耦合相干光(1) 预定波长进入平面波导(2),使得相干光的平行光通过平面波导(2)的外表面(21)的相干光的消逝场(11)传播通过平面波导(2) )。 平面波导(2)的外表面(21)包括其上能够将目标样品结合到结合位点的结合位点,使得消逝场(11)的光被与结合位点结合的靶标样物衍射。 结合位置沿着彼此平行延伸的多条预定直线(7)布置,在相邻直线之间具有恒定的距离。 预定的直线(7)相对于ev逝场(11)的传播方向成一定角度排列,使得被绑定位置绑定的目标样本衍射的相干光(12)相对于 布置在平面波导(2)的部分(10)中的另外的光耦合器(8)的直线延伸穿过平面波导传播的相干光束外部。 另外的光耦合器(8)将衍射的相干光(13)耦合到平面波导(2)之外,以便以预定的检测位置(9)干涉光路长度的差异,该光程长度是预定的 波长。
    • 8. 发明公开
    • DEVICE FOR USE IN THE DETECTION OF BINDING AFFINITIES
    • 设备中使用IN的结合亲和力识别
    • EP2929326A1
    • 2015-10-14
    • EP13799537.9
    • 2013-12-03
    • F.Hoffmann-La Roche AG
    • FATTINGER, Christof
    • G01N21/77
    • G01N21/75G01N21/7703G01N21/774G01N2021/752G01N2021/7776G01N2021/7793G01N2201/0438
    • A device for use in the detection of binding affinities, the device comprising a planar waveguide (2) arranged on a substrate (3), and further comprising an optical coupler (41) having a predetermined length for coupling coherent light (1) of a predetermined wavelength into the planar waveguide (2) such that a parallel beam of coherent light propagates through the planar waveguide (2) with an evanescent field (11) of the coherent light propagating along an outer surface (21) of the planar waveguide (2). The outer surface (21) of the planar waveguide (2) comprises binding sites thereon capable of binding target samples to the binding sites such that light of the evanescent field (11) is diffracted by target samples bound to the binding sites. The binding sites are arranged along a plurality of predetermined straight lines (7) running parallel to one another with a constant distance between adjacent straight lines. The predetermined straight lines (7) are arranged at an angle relative to the direction of propagation of the evanescent field (11) such that the coherent light (12) diffracted by the target samples bound to the binding sites impinges under a diffraction angle relative to the straight lines onto a further optical coupler (8) arranged in a portion (10) of the planar waveguide (2) outside the beam of coherent light propagating through the planar waveguide. The further optical coupler (8) couples the diffracted coherent light (13) out of the planar waveguide (2) such as to interfere at a predetermined detection location (9) with a difference in optical path length which is an integer multiple of the predetermined wavelength.