专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2703501A1 Reagents and Methods for PCR 审中-公开
标题翻译： Reagenzien und VerfahrenfürPCR
公开(公告)号：EP2703501A1
公开(公告)日：2014-03-05
申请号：EP13195156.8
申请日：2010-03-11
申请人： BRANDEIS UNIVERSITY
发明人： Wangh, Lawrence J. , Rice, John , Rice, Nicholas , Jia, Yanwei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q2545/114 , C12Q2531/107 , C12Q2527/107
摘要： Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least 32°C, and that include 2-4 modifying groups, each covalently attached to a different terminal region, preferably to a terminal nucleotide, said modifying groups being polycyclic substituents that do not have bulky portions that are non-planar, said modified olgonucleotide being capable of binding to the 5' exonuclease domains of DNA polymerases and, when included in a PCR or other primer-dependent DNA amplification reaction at a concentration, generally not more than 2000 nM, that is effective for at least one of the functions of suppressing mispriming, increasing polymerase selectivity against 3' terminal mismatches, increasing polymerase selectivity against AT-rich 3' ends, reducing scatter among replicates, suppressing polymerase 5' exonuclease activity, and inhibiting polymerase activity; as well as amplification reaction mixtures containing such modified double-stranded oligonucleotides, and amplification reactions, amplification assays and kits that include such modified double-stranded oligonucleotides.
摘要翻译：修饰的双链寡核苷酸在其每条链上具有长度为6-50个核苷酸的长度为6-50个核苷酸的末端区域，其熔解温度Tm至少为32℃，并且包括2-4个修饰基团共价连接到不同的末端区域，优选连接到末端核苷酸，所述修饰基团是不具有非平面的庞大部分的多环取代基，所述修饰的寡核苷酸能够结合DNA聚合酶的5'核酸外切酶结构域，当包含在浓度通常不超过2000nM的PCR或其他引物依赖性DNA扩增反应中时，对于抑制错配的功能中的至少一个功能是有效的，增加聚合酶对3'末端错配的选择性，增加聚合酶选择性抗AT丰富的3'末端，减少重复之间的分散，抑制聚合酶5'核酸外切酶活性，抑制聚合酶活性; 以及含有这种修饰的双链寡核苷酸的扩增反应混合物，以及包括这种修饰的双链寡核苷酸的扩增反应，扩增测定和试剂盒。

IPRDB

热门服务

关于我们

友情链接

联系方式