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1. 发明公开

EP0876500A1 COLD-ACTIVE PROTEASE CP70 失效
标题翻译： KÄLTEAKTIVEPROTEASE CP70
公开(公告)号：EP0876500A1
公开(公告)日：1998-11-11
申请号：EP97903957.0
申请日：1997-01-24
申请人： THE PROCTER & GAMBLE COMPANY , Japan Advanced Institute of Science and Technology
发明人： HASAN, A.K.M. , TAMIYA, Eiichi
IPC分类号： A23J3 , C07K14 , C11D3 , C12N9 , C12P21 , C12R1
CPC分类号： A23J3/341 , C12N9/52
摘要： A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable at a pH in the range of 6.5 to 10.0 at 30 °C for 1 hour; (d) optimal temperature: about 40 °C; (e) temperature stability: at pH 7 for 1 hour, the protease is scarcely inactivated at a temperature up to 30 °C, but it is inactivated at 40 °C as much as about 40 % and completely inactivated at 50 °C in about 10 minutes; (f) enzyme activity: the protease has about 50 % or more of its maximum activity at 20 °C; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.

2. 发明公开

EP1007722A1 COLD-ACTIVE PROTEASE CP-58 AND PSYCHROTROPHIC BACTERIA 失效
标题翻译：冷活性蛋白酶CP-58和PSYCHROTROPHISCHE细菌
公开(公告)号：EP1007722A1
公开(公告)日：2000-06-14
申请号：EP97905991.2
申请日：1997-02-14
申请人： THE PROCTER & GAMBLE COMPANY , Japan Advanced Institute of Science and Technology
发明人： HASAN, A. K. M. Quamrul , TAMIYA, Eiichi
IPC分类号： C12P21/04 , C12N9/52 , A61K38/00 , A23J1/00
CPC分类号： C12Y304/00 , C11D3/386 , C12N9/52
摘要： A cold-active protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity: the protease acts on casein, gelatin, albumin and hemoglobin to specifically decompose them in the order of casein, gelatin, albumin and hemoglobin; (b) optimal pH: 7.5 to 8.0; (c) pH stability: the protease is stable at a pH in the range of 5.5 to 10.5 at 20 °C for 1 hour; (d) optimal temperature: 20 °C at pH 10.5 and 40 °C at pH 8.0; (e) temperature stability: at pH 10.5 for 1 hour, the protease is scarcely inactivated at a temperature of 10 °C to 30 °C, but it is inactivated at 40 °C as much as about 30 % and completely inactivated at 50 °C; (f) enzyme activity: the protease has about 60 % or more of its maximum activity at 20 °C; (g) the active center of the enzyme is a metallic ion; and (h) the molecular weight of the protease is about 58 kDa as measured by SDS-PAGE.

3. 发明公开

EP1734360A1 PLASMA GENERATING EQUIPMENT 有权
标题翻译：等离子切割机Verfahren undGerät
公开(公告)号：EP1734360A1
公开(公告)日：2006-12-20
申请号：EP05721448.8
申请日：2005-03-25
申请人： Japan Advanced Institute of Science and Technology
发明人： TAKAMURA, Yuzuru , IIDUKA, Akiko , TAMIYA, Eiichi
IPC分类号： G01N21/71
CPC分类号： G01N21/68 , G01N21/67 , G01N21/69
摘要： A method for generating plasma and a method for elemental analysis, each comprising the steps of providing a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; filling the flow channel and the narrow portion with a conductive liquid, and thereafter applying an electric field to the narrow portion, to conduct the electric field through the narrow portion, thereby generating plasma at the narrow portion. An apparatus for generating plasma, the apparatus for generating plasma comprising a narrow portion in a flow channel made of an insulation material, the narrow portion having a cross-sectional area markedly smaller than a cross-sectional area of the flow channel; and a means of applying an electric field to the narrow portion to conduct the electric field through the narrow portion; and an apparatus for emission spectroscopic analysis comprising the apparatus for generating plasma as defined above.
摘要翻译：一种用于产生等离子体的方法和元素分析方法，每个方法包括以下步骤：在由绝缘材料制成的流动通道中提供窄部分，所述窄部分的横截面积明显小于所述绝缘材料的横截面面积流通道用导电液体填充流动通道和狭窄部分，然后向窄部分施加电场，以使电场通过狭窄部分，从而在狭窄部分产生等离子体。一种用于产生等离子体的装置，用于产生等离子体的装置包括由绝缘材料制成的流动通道中的窄部分，所述窄部分具有明显小于所述流动通道的横截面积的横截面面积; 以及向狭窄部分施加电场以通过所述狭窄部分传导电场的装置; 以及包括如上所述产生等离子体的装置的发射光谱分析装置。

4. 发明授权

EP1734360B1 Plasma generating method and equipment 有权
标题翻译：等离子体产生的方法和设备
公开(公告)号：EP1734360B1
公开(公告)日：2014-05-07
申请号：EP05721448.8
申请日：2005-03-25
申请人： Japan Advanced Institute of Science and Technology
发明人： TAKAMURA, Yuzuru , IIDUKA, Akiko , TAMIYA, Eiichi
IPC分类号： G01N21/71 , G01N21/73 , G01N21/67 , G01N21/68
CPC分类号： G01N21/68 , G01N21/67 , G01N21/69

5. 发明公开

EP1962093A1 ANALYZER AND ANALYSIS METHOD 有权
标题翻译：分析仪与分析方法
公开(公告)号：EP1962093A1
公开(公告)日：2008-08-27
申请号：EP06833421.8
申请日：2006-11-27
申请人： Japan Science and Technology Agency , Japan Advanced Institute of Science and Technology , Bio Device Technologies Co., Ltd.
发明人： NAGATANI, Naoki , YUI, Teruko , TAMIYA, Eiichi , ENDOU, Tatsurou , TANAKA, Ryou
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , Y10S435/97
摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR
SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.
摘要翻译： [问题]为了减少例如捕获抗体的使用量而不降低检测灵敏度。同时，即使在仅积聚少量标记抗体的情况下，也能够在判定区域中实现强烈的显色或发光。降低夹心法中的检测限。在比赛方法中扩大动态范围。解决问题的手段本发明提供一种使用受试物质通过免疫学分析方法分析受试物质的方法，具有测定区域的载体，其上选自能够与测试物质特异性结合的捕获抗体和已经固定了捕获能够与被检物质特异性结合的抗原，以及能够与被检物质特异性结合的标记抗体，其中具有增敏作用的标记已经被固定在支持物的测定区域上。

6. 发明授权

EP1962093B1 ANALYSIS METHOD 有权
标题翻译：分析程序
公开(公告)号：EP1962093B1
公开(公告)日：2013-06-12
申请号：EP06833421.8
申请日：2006-11-27
申请人： Japan Science and Technology Agency , Japan Advanced Institute of Science and Technology , Bio Device Technologies Co., Ltd.
发明人： NAGATANI, Naoki , YUI, Teruko , TAMIYA, Eiichi , ENDOU, Tatsurou c/o Tokyo Institute of Technology , TANAKA, Ryou
IPC分类号： G01N33/543
CPC分类号： G01N33/54393 , Y10S435/97

7. 发明公开

EP0871719A1 PSYCHROPHILIC PROTEASE AND PSYCHROPHILIC BACTERIA 失效
标题翻译：嗜冷蛋白酶和细菌
公开(公告)号：EP0871719A1
公开(公告)日：1998-10-21
申请号：EP96906484.0
申请日：1996-02-16
申请人： THE PROCTER & GAMBLE COMPANY
发明人： TAMIYA, Eiichi
IPC分类号： C12N9 , C12P21
CPC分类号： C12R1/20 , C12N9/52
摘要： A novel psychrophilic protease and a microorganism having the psychrophilic protease producing ability are disclosed. The protease acts on and decomposes casein and dimethylcasein but not on ribonuclease, has an optimal temperature of about 40 °C. Under the condition of storage at pH 7 for 1 hour, it is inactivated scarcely at a temperature up to 30 °C, but at 40 °C it loses about 40 % of the activity. At 50 °C, the protease is rapidly inactivated so that the activity is completely abolished in about 15 minutes. Flavobacterium balustinum having the protease producing ability is also disclosed.

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