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1. 发明公开

EP1142998A1 METHOD OF BREEDING YEAST 有权
标题翻译： VERFAHREN ZURZÜCHTUNGVON HEFE
公开(公告)号：EP1142998A1
公开(公告)日：2001-10-10
申请号：EP00970088.1
申请日：2000-10-26
申请人： SUNTORY LIMITED
发明人： ASHIKARI, Toshihiko , OCHIAI, Misa
IPC分类号： C12N15/11 , C12N1/19 , C12C11/02
CPC分类号： C12C12/004 , C12C12/006 , C12N15/81 , C12R1/85
摘要： A DNA construct comprising:

(1) a selective marker gene,
(2) a galactose-inducible growth inhibition sequence,
(3) a pair of FRT sequences in the same orientation flanking (1) and (2), and
(4) a DNA fragment capable of recombining with a yeast chromosomal DNA located at each end of (3), wherein said FRT sequences contain the following sequence: or a sequence substantially identical to said sequence, provided that in each member of said pair of FRT sequences, the inverted repeat distal from the flanked selective marker gene and growth inhibition sequence has at least one but no more than six nucleotides deleted on the side distal from the spacer sequence; a method for transforming a yeast of the genus Saccharomyces with said DNA construct; a yeast of the genus Saccharomyces transformed by said method; and a method for producing a beer comprising using said yeast of the genus Saccharomyces.
摘要翻译：一种DNA构建体，其包含：（1）选择性标记基因，（2）半乳糖诱导型生长抑制序列，（3）在侧翼（1）和（2）中相同取向的一对FRT序列，和（4）a 能够与位于（3）的每个末端的酵母染色体DNA重组的DNA片段，其中所述FRT序列包含以下序列：图像或与所述序列基本相同的序列，条件是在所述一对FRT的每个成员中序列，来自侧翼选择性标记基因的远端的反向重复和生长抑制序列在远离间隔物序列的一侧具有至少一个但不超过六个核苷酸; 用所述DNA构建体转化酵母属酵母的方法; 通过所述方法转化的酵母属酵母; 以及包含使用酵母属酵母的酿造啤酒的方法。

2. 发明公开

EP1784507A2 METHOD FOR ANALYZING GENES OF INDUSTRIAL YEASTS 审中-公开
标题翻译：程序的基因从培养酵母分析
公开(公告)号：EP1784507A2
公开(公告)日：2007-05-16
申请号：EP05783945.8
申请日：2005-08-30
申请人： SUNTORY LIMITED
发明人： ASHIKARI, Toshihiko , NAKAO, Yoshihiro , NAKAMURA, Norihisa , KODAMA, Yukiko , FUJIMURA, Tomoko
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , C12Q2600/156 , C12Q2600/158
摘要： An object of the present invention is to provide a method for analyzing gene of an industrial yeast. The method of the present invention comprises (a) analyzing the genome sequence of the industrial yeast; and (c-1) selecting a gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of Saccharomyces cerevisiae, or (c-2) selecting a gene of the industrial yeast consisting of a nucleotide sequence having 60 to 94% identity to the nucleotide sequence of the gene of Saccharomyces cerevisiae.

3. 发明公开

EP1599605A1 SCREENING METHOD FOR GENES OF BREWING YEAST 有权
标题翻译：巴斯德酵母SSU1基因，多肽，以及它们用于醇的制备中使用
公开(公告)号：EP1599605A1
公开(公告)日：2005-11-30
申请号：EP04716716.8
申请日：2004-03-03
申请人： SUNTORY LIMITED
发明人： NAKAO, Yoshihiro , NAKAMURA, Norihisa , KODAMA, Yukiko , FUJIMURA, Tomoko , ASHIKARI, Toshihiko
IPC分类号： C12Q1/68 , C12C12/00 , C12N15/54 , B01L3/00
CPC分类号： C12N15/1034 , C07K14/395 , C12C12/004 , C12C12/006 , C12N1/18 , C12P7/06 , C12Q1/6895 , C12Q2600/156 , C12Q2600/158 , Y02E50/17
摘要： An object of the present invention is to provide a method for the selection of gene participating in the desired brewing character in such a manner that a database compiling the data of the whole genome sequence of industrial yeast, particularly of a brewing yeast used for alcoholic beverages such as beer is prepared; gene participating in a brewing character that the brewing yeast, specifically possesses is selected from the database; and functional analysis of the gene is carried out by disruption or overexpression, and to provide a DNA array (in which oligonucleotide(s) selected) based on the database compiling the data of the whole genome sequences of an industrial yeast or, particularly, of a brewing yeast (is/are adhered on a solid plate). Another object is to provide a method for breeding of yeast achieving the brewing character which the gene participates in, and also a method for the production of an alcohol or an alcoholic beverage in which productivity and quality are improved using the yeast. Still another object is to provide a gene which is specific to the brewing yeast and a peptide encoded by the gene.Means for achieving the above objects is a screening method for genes participating in increase in productivity and/or improvement in flavor in the production of an alcohol or an alcoholic beverage, characterized in that, (A) the whole genome sequence of industrial yeast is analyzed, (B) the genome sequence is compared with the whole genome sequence of S. cerevisiae, (C) gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of S. cerevisiae is selected and (D) functional analysis of the gene is carried out, whereby the character which is given to the yeast by the gene is identified.

4. 发明公开

EP0810287A1 GENES CODING FOR PROTEINS HAVING ACYL TRANSFER ACTIVITY 失效
标题翻译： GENE，DIEFÜR蛋白质麻省理工学院ACYLTRANSFERASEAKTIVITÄTKODIEREN
公开(公告)号：EP0810287A1
公开(公告)日：1997-12-03
申请号：EP96902462.9
申请日：1996-02-16
申请人： SUNTORY LIMITED
发明人： ASHIKARI, Toshihiko , TANAKA, Yoshikazu , FUJIWARA, Hiroyuki , NAKAO. Masahiro , FUKUI, Yuko , SAKAKIBARA, Keiko , MIZUTANI, Masako , KUSUMI, Takaaki
IPC分类号： C12N15/54 , C12N9/10 , C12N1/21 , C12N5/00 , A01H5/00
CPC分类号： C07H15/203 , C12N9/1029 , C12N15/825 , C12P19/445
摘要： Proteins having an aromatic-acyltransferase activity; a gene system coding for the same; a process for producing the proteins using the gene system; and uses of the genes and the proteins. The genes and the proteins acylate plant pigments such as anthocyanin to thereby cause color tone changes, thus providing plants, particularly flowers, having colors that have not been inherent therein.
摘要翻译：具有芳香 - 酰基转移酶活性的蛋白质; 编码该基因的基因系统; 使用该基因系统产生蛋白质的方法; 以及基因和蛋白质的使用。基因和蛋白质使植物颜料如花青素酰化，从而引起色调变化，从而提供植物，特别是具有尚未固有的颜色的植物。

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