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1. 发明公开

EP4001406A2 RECOMBINANT MICROORGANISM HAVING INCREASED ABILITY TO PRODUCE HYDROPHOBIC MATERIAL AND CELL-MEMBRANE ENGINEERING METHOD FOR PREPARATION THEREOF 无效
公开(公告)号：EP4001406A2
公开(公告)日：2022-05-25
申请号：EP21205995.0
申请日：2021-11-02
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YANG, Dongsoo , PARK, Seon Young
IPC分类号： C12N9/10 , C12P23/00 , C12P17/16 , C07K14/705 , C12N9/04 , C12N9/90 , C12N1/20 , C12N15/52
摘要： Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

2. 发明公开

EP3884951A1 COMPOSITION FOR TREATING HAIR LOSS OR PROMOTING HAIR GROWTH 审中-公开
公开(公告)号：EP3884951A1
公开(公告)日：2021-09-29
申请号：EP19886811.9
申请日：2019-11-21
申请人： Core Innovations Limited , Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YOO, Won Min , KIM, Sooncheol , CHUNG, Hannah , NA, Deok Hyun
IPC分类号： A61K35/16 , A61K38/17 , A61P17/14 , A61K8/98 , A61K8/64 , A61Q7/00
摘要： The present invention relates to a composition for treating hair loss or promoting hair growth which contains a silk protein, and plasma or serum components. According to the present invention, due to the synergistic effect of a silk protein and plasma or serum components, the effect of promoting hair growth and the effect of treating hair loss can be expected to be more efficient.

3. 发明公开

EP3741860A1 VARIANT MICROORGANISM HAVING ABILITY TO PRODUCE 1,3-PROPANEDIOL, AND METHOD FOR PREPARING 1,3-PDO BY USING SAME 审中-公开
公开(公告)号：EP3741860A1
公开(公告)日：2020-11-25
申请号：EP19740842.0
申请日：2019-01-15
申请人： Korea Advanced Institute Of Science And Technology , Hanwha Solutions Corporation
发明人： LEE, Sang Yup , CHO, Jae Sung , KIM, Je Woong , KO, Yoo Sung , PRABOWO, Cindy Pricilia Surya , HAN, Taehee , KIM, Euiduk , CHOI, Jae Won , CHO, Changhee , LEE, Jun Kyu
IPC分类号： C12N15/77 , C12P7/18 , C07K14/245 , C12N9/04 , C12N9/12 , C12N9/88
摘要： The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.

4. 发明公开

EP3660157A9 SUCCINIC ACID-PRODUCING MUTANT MICROORGANISM INTO WHICH HIGH ACTIVITY MALATE DEHYDROGENASE IS INTRODUCED, AND METHOD FOR PREPARING SUCCINIC ACID BY USING SAME 审中-公开
公开(公告)号：EP3660157A9
公开(公告)日：2020-07-29
申请号：EP19762287.1
申请日：2019-05-02
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , KIM, Kyung-Jin , AHN, Jung Ho , SEO, Hogyun , LEE, Jong An
IPC分类号： C12N15/74 , C12N9/04 , C12P7/46
摘要： Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.

5. 发明授权

EP2281880B1 MUTANT MICROORGANISM WITH HIGH ABILITY OF PRODUCING PUTRESCINE AND PREPARATION OF PUTRESCINE USING SAME 有权
标题翻译：具有高腐胺生产能力和生产腐胺的突变微生物
公开(公告)号：EP2281880B1
公开(公告)日：2016-07-06
申请号：EP09729276.7
申请日：2009-03-05
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , QIAN, Zhi Gang , XIA, Xiaoxia , JEON, Yong Jae
IPC分类号： C12N15/00 , C12P13/00
CPC分类号： C12P13/001

6. 发明公开

EP2803727A2 NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME 有权
标题翻译：合成新法规斯尔纳和方法及其生产
公开(公告)号：EP2803727A2
公开(公告)日：2014-11-19
申请号：EP13735942.8
申请日：2013-01-11
申请人： Korea Advanced Institute Of Science And Technology
发明人： LEE, Sang Yup , NA, Dokyun , YOO, Seung Min
IPC分类号： C12N15/113 , C12N15/63 , C12N1/21
CPC分类号： C12N15/1138 , C12N15/111 , C12N15/63 , C12N15/67 , C12N15/70 , C12N2310/11 , C12N2310/13 , C12N2310/18 , C12N2310/3519 , C12N2310/531 , C12P7/22 , C12P13/001 , C12P13/225
摘要： The present invention relates to a novel customized sRNA that reduces gene expression in prokaryotic cells, a preparation method thereof, and the use thereof, and more particularly to a synthetic sRNA comprising an Hfq binding site, derived from the sRNA of any one of MicC, SgrS and MicF, and a region that base-pairs with the target gene mRNA, and to a preparation method thereof and the use thereof. The synthetic sRNA according to the invention has an advantage in that the degree of inhibition of the target gene can be controlled by regulating the ability of the synthetic sRNA to bind to the mRNA of the target gene. The use of the synthetic sRNA that regulates the expression of the target gene makes it possible to effectively construct a recombinant microorganism without using a conventional gene deletion method and to reduce the expression of the target gene, and thus the synthetic sRNA is useful for the production of recombinant microorganisms. Also, the synthetic sRNA can be quickly applied to various strains, and thus is very suitable for the measurement of metabolic capabilities of strains and the selection of the most suitable strain. In addition, recombinant microorganisms, which are obtained by metabolic flux manipulation using the synthetic sRNA and produce tyrosine or cadaverine with high efficiency, are useful in the drug and industrial fields. In other words, the use of the sRNA according to the present invention can make it easy to select target genes whose expression is to be inhibited for the highly efficient production of metabolites. Accordingly, the synthetic sRNA can be used to construct recombinant strains for efficient production of various metabolites and to establish efficient methods for production of various metabolites, and thus is highly useful.

7. 发明公开

EP2330186A2 METHOD FOR SYNTHESIZING PROTEIN CONTAINING HIGH CONTENT OF SPECIFIC AMINO ACID THROUGH SIMULTANEOUS EXPRESSION WITH TRNA OF THE SPECIFIC AMINO ACID 有权
标题翻译：合成与特定的氨基比例较高的蛋白质BY同时表达与特定的tRNA氨基方法
公开(公告)号：EP2330186A2
公开(公告)日：2011-06-08
申请号：EP09814823.2
申请日：2009-09-22
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , XIA, Xiaoxia , QIAN, Zhi Gang , BAEK, Jong Hwan
IPC分类号： C12N1/21
CPC分类号： C12N15/67 , C07K14/43518 , C07K14/43586
摘要： The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.
摘要翻译：本发明涉及由编码具有特定氨基酸的用编码特定氨基酸的tRNA的核苷酸序列的含量高的靶蛋白的基因的共表达增加靶蛋白的表达的方法。。根据本发明，具有特定氨基酸的含量高的蛋白的表达可显着通过与特定的氨基酸的tRNA的联合表达增加。因此，本发明是一种用于提高具有特定氨基酸的含量高，检查作为重复蛋白的蛋白质的生产率是有用的。

8. 发明公开

EP2314638A2 METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF 审中-公开
标题翻译：用于生产聚乳酸及其共聚物通过改进聚乳酸与能力COPOLYMERERZEUGENDER突变的微生物手段
公开(公告)号：EP2314638A2
公开(公告)日：2011-04-27
申请号：EP09770374.8
申请日：2009-06-24
申请人： LG Chem, Ltd. , Korea Advanced Institute of Science and Technology
发明人： YANG, Taek Ho , PARK, Si Jae , KIM, Tae Wan , LEE, Sang Yup , JUNG, Yu Kyung
IPC分类号： C08G63/91
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

9. 发明公开

EP2038403A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：支链氨基酸提高生产能力的突变微生物及用其制备支链氨基酸的方法
公开(公告)号：EP2038403A1
公开(公告)日：2009-03-25
申请号：EP07715659.4
申请日：2007-03-14
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产率的突变微生物以及使用该突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码参与L-亮氨酸的酶的基因和编码L-异亮氨酸生物合成酶的基因而产生的L-缬氨酸的生产率提高的突变微生物参与D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，从而增加其表达，以及使用该突变体微生物生产L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变微生物可高效生产支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

10. 发明公开

EP4209587A3 RECOMBINANT MICROORGANISM HAVING INCREASED ABILITY TO PRODUCE HYDROPHOBIC MATERIAL AND CELL-MEMBRANE ENGINEERING METHOD FOR PREPARATION THEREOF 审中-实审
公开(公告)号：EP4209587A3
公开(公告)日：2023-10-11
申请号：EP23154792.8
申请日：2021-11-02
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YANG, Dongsoo , PARK, Seon Young
IPC分类号： C12N9/10 , C12P23/00 , C12P17/16 , C07K14/705 , C12N9/04 , C12N9/90 , C12N1/20 , C12N15/52
摘要： Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

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