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    • 1. 发明公开
    • BIOLOGICAL SUBSTANCE QUANTITATION METHOD, PATHOLOGICAL DIAGNOSIS SUPPORT SYSTEM, AND PROGRAM
    • 生物物质定量方法,病理诊断支持系统和程序
    • EP3249401A1
    • 2017-11-29
    • EP16740073.8
    • 2016-01-15
    • Konica Minolta, Inc.
    • ISODA, TakeshiFURUSAWA, NaokoMOTOKUI, YasuyukiGONDA, KohsukeOHUCHI, NoriakiWATANABE, Mika
    • G01N33/53G01N33/48G01N33/536G01N33/58G01N33/68
    • Provided are: a biological substance quantitation method with which the expression level of a nucleoprotein in a specimen can be accurately quantitated; a pathological diagnosis support system; and a program. This biological substance quantitation method, in which a biological substance is quantitated, on the basis of the fluorescence of a fluorescent substance, from a specimen that has been stained using a staining reagent that includes fluorescent particles containing said fluorescent substance, is characterized by including an input step for inputting a fluorescent image that represents the expression of the biological substance in the specimen using a fluorescent bright spot, and a quantitation step for quantitating the expression level of the biological substance on the basis of the fluorescence of the fluorescent bright spot, wherein the biological substance is a nucleoprotein that is expressed in a cell nucleus, and the fluorescent particles bind to the biological substance via a primary antibody for which the biological substance serves as an antigen.
    • 本发明提供一种生物物质定量方法,能够准确地定量样本中的核蛋白的表达量, 病理诊断支持系统; 和一个程序。 该基于荧光物质的荧光定量生物物质的生物物质定量方法,其特征在于,使用包含含有所述荧光物质的荧光粒子的染色试剂对已被染色的样本进行定量,其特征在于, 输入步骤,用于使用荧光亮点输入表示生物物质在样本中的表达的荧光图像;以及定量步骤,用于基于荧光亮点的荧光来定量生物物质的表达水平,其中 生物物质是在细胞核中表达的核蛋白,荧光颗粒经由生物物质作为抗原的一次抗体与生物物质结合。