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1. 发明授权

EP1592812B1 BIOMOLECULE ANALYSIS BY ROLLING CIRCLE AMPLIFICATION AND SERS DETECTION 有权
标题翻译： BIOMOLEKÜLANALYSE通过滚环扩增和SERS检测
公开(公告)号：EP1592812B1
公开(公告)日：2009-04-22
申请号：EP04775738.0
申请日：2004-01-28
申请人： Intel Corporation
发明人： SUN, Lei , SU, Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6816 , C12Q1/682 , G01N21/658 , C12Q2565/632 , C12Q2565/518 , C12Q2531/125 , C12Q2563/179

2. 发明公开

EP1592812A2 BIOMOLECULE ANALYSIS BY ROLLING CIRCLE AMPLIFICATION AND SERS DETECTION 有权
标题翻译： BIOMOLEKÜLANALYSE通过滚环扩增和SERS检测
公开(公告)号：EP1592812A2
公开(公告)日：2005-11-09
申请号：EP04775738.0
申请日：2004-01-28
申请人： INTEL CORPORATION
发明人： SUN, Lei , SU, Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , C12Q1/6816 , C12Q1/682 , G01N21/658 , C12Q2565/632 , C12Q2565/518 , C12Q2531/125 , C12Q2563/179
摘要： The present methods, compositions and systems are concerned with biomolecule 130 detection, identification and/or quantification by rolling circle amplification (RCA) and Raman detection. In particular embodiments of the invention, the RCA is exponential RCA or linear RCA. In some embodiments of the invention, the Raman detection is SERS or SERRS. The circular DNA template 150, 210, 310 to be amplified may comprise one or more polythymidine 320 residues, resulting in amplification products 170, 230, 250, 330, 410 containing multiple polyadenylate 340, 420 residues. The polyadenylates 340, 420 may be directly detected by Raman detection. Alternatively, one or more Raman labels may be incorporated into the amplification products 170, 230, 250, 330, 410 to facilitate Raman detection. Because of the amplification produced by LRCA or ERCA and the enhanced Raman signal produced by multiple polyadenylates 340, 420 and/or Raman labels, detection of single copy biomolecules 130 is feasible using the disclosed methods, compositions and/or systems.

3. 发明公开

EP1699938A2 METHODS OF PRODUCING CARBON NANOTUBES USING PEPTIDE OR NUCLEIC ACID MICROPATTERNING 审中-公开
标题翻译：工艺制造碳纳米管的使用肽或核酸MICRO图案的生产
公开(公告)号：EP1699938A2
公开(公告)日：2006-09-13
申请号：EP04818073.1
申请日：2004-12-24
申请人： INTEL CORPORATION
发明人： SU, Xing , SUNDARARAJAN, Narayan , YAMAKAWA, Mineo , BERLIN, Andrew , SUN, Lei , ZHANG, Yuegang
IPC分类号： C12Q1/68
CPC分类号： B82Y40/00 , B82Y10/00 , B82Y30/00 , C01B32/162 , C01B2202/08 , C01B2202/36 , D01F9/127 , H01L51/0048 , H01L51/0052
摘要： The methods, apparatus and systems disclosed herein concern ordered arrays of carbon nanotubes. In particular embodiments of the invention, the nanotube arrays are formed by a method comprising attaching catalyst nanoparticles (140, 230) to polymer (120, 210) molecules, attaching the polymer (120, 210) molecules to a substrate, removing the polymer (120, 210) molecules and producing carbon nanotubes on the catalyst nanoparticles (140, 230). The polymer (120, 210) molecules alignment techniques. The nanotube arrays can be attached to selected areas (110, 310) of the substrate. Within the selected areas (110, 310), the nanotubes are distributed non-randomly. Other embodiments disclosed herein concern apparatus that include ordered arrays of nanotubes attached to a substrate and systems that include ordered arrays of carbon nanotubes attached to a substrate, produced by the claimed methods. In certain embodiments, provided herein are methods for aligning a molecular wire, by ligating the molecular wire to a double stranded DNA molecule.

4. 发明公开

EP1711829A2 METHODS FOR USING RAMAN SPECTROSCOPY TO OBTAIN A PROTEIN PROFILE OF A BIOLOGICAL SAMPLE 审中-公开
标题翻译：用拉曼光谱对蛋白质的保护方法PROFILE生物样品
公开(公告)号：EP1711829A2
公开(公告)日：2006-10-18
申请号：EP04815772.1
申请日：2004-12-29
申请人： Intel Corporation
发明人： BERLIN, Andrew , SU, Xing , CHAN, Selena , KOO, Tae-Woong , SUN, Lei , SUNDARARAJAN, Narayan , YAMAKAWA, Mineo
IPC分类号： G01N33/58
CPC分类号： G01N21/658 , G01N33/6803 , G01N2021/653 , G01N2021/655 , G01N2021/656
摘要： The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

5. 发明公开

EP1668164A2 PROGRAMMABLE MOLECULAR BARCODES 审中-公开
标题翻译：可编程分子条形码
公开(公告)号：EP1668164A2
公开(公告)日：2006-06-14
申请号：EP04816244.0
申请日：2004-09-23
申请人： INTEL CORPORATION
发明人： SU, Xing , KOO, Tae-Woong , BERLIN, Andrew , SUN, Lei , SUNDARARAJAN, Narayanan , YAMAKAWA, Mineo
IPC分类号： C12Q1/68
CPC分类号： B82Y10/00 , B82Y5/00 , C12Q1/6816 , G06K19/06028 , C12Q2525/161 , C12Q2537/143 , C12Q2565/1025
摘要： The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind to a target, such as proteins, nucleic acids and other biomolecules or aggregates. Different barcodes may be distinguished by the type and location of the tags. In other embodiments, barcodes may be produced by hybridization of one or more tagged oligonucleotides to a template, comprising a container section and a probe section. The tagged oligonucleotides may be designed as modular code sections, to form different barcodes specific for different targets. In alternative embodiments, barcodes may be prepared by polymerization of monomeric units. Bound barcodes may be detected by various imaging modalities, such as, surface plasmon resonance, fluorescent or Raman spectroscopy.

6. 发明公开

EP1606610A2 CHEMICAL ENHANCEMENT IN SURFACE ENHANCED RAMAN SCATTERING USING LITHIUM CHLORIDE 审中-公开
标题翻译： FOR增强拉曼散射表面化学BOOST使用氯化锂
公开(公告)号：EP1606610A2
公开(公告)日：2005-12-21
申请号：EP04749311.9
申请日：2004-02-04
申请人： INTEL CORPORATION
发明人： SU, Xing , SUN, Lei , KOO, Tae-Woong , CHAN, Selana
IPC分类号： G01N21/65
CPC分类号： G01J3/44 , B82Y15/00 , C12Q1/6825 , G01N21/658
摘要： The intensity of the signals from surface enhanced Raman spectroscopy is increased by using lithium chloride (122) as an enhancer to activate a metallic structure (212) used for surface enhanced Raman spectroscopy (116). The increased signal intensity allows surface enhanced Raman spectroscopy to be utilized to detect individual analytes (120) such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

7. 发明公开

EP1718764A1 A METHOD AND DEVICE FOR USING RAMAN-ACTIVE PROBE CONSTRUCTS TO ASSAY BIOLOGICAL SAMPLES 审中-公开
标题翻译： FOR使用拉曼活性探针的方法和设备构建了测试生物样品
公开(公告)号：EP1718764A1
公开(公告)日：2006-11-08
申请号：EP04816064.2
申请日：2004-12-28
申请人： Intel Corporation
发明人： BERLIN, Andrew , SU, Xing , CHAN, Selena , KOO, Tae-Woong , SUN, Lei
IPC分类号： C12Q1/68 , G01N21/55 , G01N21/65 , G01N33/553 , G01J3/44
CPC分类号： G01N21/658 , B82Y5/00 , B82Y10/00 , B82Y20/00 , C12Q1/6816 , C12Q1/6834 , G01N33/543 , G01N33/68 , G01N33/6818 , G01N2021/653 , G01N2021/655 , G01N2021/656 , C12Q2565/125 , C12Q2565/632
摘要： Various methods of using Raman-active or SERS-active probe constructs to detect analytes in biological samples, such as the protein-containing analytes in a body fluid are provided. The probe moieties in the Raman-active constructs are selected to bind to and identify specific known analytes in the biological sample or the probe moieties are designed to chemically interact with functional groups commonly found in certain amino acids so that the invention methods provide information about the amino acid composition of protein-containing analytes or fragments in the samples. In some cases, the Ramanactive or SERS-active probe constructs, when used in the invention methods, can identify particular protein-containing analytes or types of such analytes so that a protein profile of a patient sample can be made. When compared to a data base of Raman or SERS spectra of normal samples, a disease state of a patient can be identified using the methods disclosed.

8. 发明公开

EP1711626A2 NUCLEIC ACID SEQUENCING BY RAMAN MONITORING OF UPTAKE OF NUCLEOTIDES DURING MOLECULAR REPLICATION 审中-公开
标题翻译：拉曼监测分子复制过程中核苷酸摄取的核酸序列分析
公开(公告)号：EP1711626A2
公开(公告)日：2006-10-18
申请号：EP04821053.8
申请日：2004-12-28
申请人： INTEL CORPORATION
发明人： BERLIN, Andrew , SU, Xing , CHAN, Selena , KOO, Tae-Woong , SUN, Lei , SUNDARARAJAN, Narayan
IPC分类号： C12Q1/68
CPC分类号： G01N21/658 , C12Q1/6869 , C12Q2565/632 , C12Q2533/101
摘要： The methods and apparatus disclosed herein are useful for detecting nucleotides, nucleosides, and bases and for nucleic acid sequence determination. The methods involve detection of a nucleotide, nucleoside, or base using surface enhanced Raman spectroscopy (SERS) or surface enhanced coherent anti-Stokes Raman spectroscopy (SECARS). The detection can be part of a nucleic acid sequencing reaction to detect uptake of a deoxynucleotide triphosphate during a nucleic acid polymerization reaction, such as a nucleic acid sequencing reaction. The nucleic acid sequence of a synthesized nascent strand, and the complementary sequence of the template strand, can be determined by tracking the order of incorporation of nucleotides during the polymerization reaction. Methods for enhancing the SERS signal of a nucleotide or nucleoside by cleaving the base from a sugar moiety are provided. Furthermore, methods for detecting single base repeats are provided.
摘要翻译：本文公开的方法和装置可用于检测核苷酸，核苷和碱基并用于核酸序列测定。该方法涉及使用表面增强拉曼光谱（SERS）或表面增强相干反斯托克斯拉曼光谱（SECARS）检测核苷酸，核苷或碱基。检测可以是核酸测序反应的一部分，以在核酸聚合反应（例如核酸测序反应）期间检测脱氧核苷酸三磷酸的摄取。合成的新生链的核酸序列和模板链的互补序列可以通过追踪聚合反应期间核苷酸掺入的顺序来确定。提供了通过从糖部分切割碱基来增强核苷酸或核苷的SERS信号的方法。此外，提供了检测单碱基重复的方法。

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