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1. 发明公开

EP3036334A1 A SOYBEAN U6 POLYMERASE III PROMOTER AND METHODS OF USE 审中-公开
标题翻译：索非亚N6-POLYMERASE-III-PROMOTOR UND VERFAHREN ZUR VERWENDUNG
公开(公告)号：EP3036334A1
公开(公告)日：2016-06-29
申请号：EP14761479.6
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , LI, Zhongsen , LIU, Zhan-Bin , YOUNG, Joshua K.
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
摘要翻译：提供组合物和方法用于植物或植物细胞基因组中靶序列的基因组修饰。方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法的细胞或生物体。一旦确定了基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

2. 发明公开

EP3611268A1 PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 审中-公开
公开(公告)号：EP3611268A1
公开(公告)日：2020-02-19
申请号：EP19199945.7
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew , FALCO, Saverio Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, L. Aleksander , SHI, Jinrui , SVITASHEV, Sergei , YOUNG, Joshua K.
IPC分类号： C12N15/82 , C12N15/113
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

3. 发明授权

EP2643464B1 BRASSICA GAT EVENT DP-073496-4 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 有权转让
公开(公告)号：EP2643464B1
公开(公告)日：2018-12-26
申请号：EP10784933.3
申请日：2010-11-24
申请人： Pioneer Hi-Bred International, Inc. , E. I. du Pont de Nemours and Company
发明人： CHARNE, David, George , CHEN, Wenpin , KOSCIELNY, Chadwick, Bruce , PATEL, Jayantilal, Devabhai , THOONEN, Ferdinand, Gerard , TULSIERAM, Lomas , ZHANG, Yongping , LI, Zhongsen
IPC分类号： C12N15/82
CPC分类号： C12N15/8275 , C12N15/8274
摘要： Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

4. 发明公开

EP2373798A1 SITE-SPECIFIC INTEGRATION AND STACKING OF TRANSGENES IN SOYBEAN VIA DNA RECOMBINASE MEDIATED CASSETTE EXCHANGE 审中-公开
标题翻译：定点整合和转基因堆叠在大豆上的DNA重组酶介导的盒式交换
公开(公告)号：EP2373798A1
公开(公告)日：2011-10-12
申请号：EP09775058.2
申请日：2009-12-16
申请人： E. I. du Pont de Nemours and Company
发明人： FALCO, Saverio, Carl , LI, Zhongsen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8213
摘要： A targeting method is described that allows precise cassette replacement at a previously characterized genetic locus. A target DNA construct containing a pair of incompatible
FRT sites flanking a target cassette was introduced into soybean by regular biolistic transformation. Transgenic events containing a single complete copy of the target site were then selected and retransformed with a donor DNA construct containing the identical pair of incompatible
FRT sites flanking a donor cassette. Precise DNA cassette exchange happened between the target cassette and the donor cassette via recombinase mediated cassette exchange (RMCE) so that the donor cassette was introduced at the exact genomic site previously occupied by the target cassette. Through repeated RMCE using additional incompatible
FRT sites, multiple groups of transgenes can be stacked at the same genomic locus.

6. 发明公开

EP3274460A1 SOYBEAN U6 SMALL NUCLEAR RNA GENE PROMOTERS AND THEIR USE IN CONSTITUTIVE EXPRESSION OF SMALL RNA GENES IN PLANTS 审中-公开
标题翻译：大豆U6小核RNA基因启动子及其在植物中小RNA基因组成本表达中的应用
公开(公告)号：EP3274460A1
公开(公告)日：2018-01-31
申请号：EP16715935.9
申请日：2016-03-21
申请人： E. I. du Pont de Nemours and Company
发明人： LI, Zhongsen
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8213
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to promoters of U6 small nuclear RNA genes, and fragments thereof, and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

7. 发明公开

EP3036332A1 PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 有权转让
标题翻译： PFLANZENGENOMMODIFIZIERUNG MIT RNS / CAS-ENDONUKLEASEFÜHRUNGSSYSTEMENUND VERFAHREN ZUR VERWENDUNG
公开(公告)号：EP3036332A1
公开(公告)日：2016-06-29
申请号：EP14761477.0
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew , FALCO, Saverio Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, L. Aleksander , SHI, Jinrui , SVITASHEV, Sergei , YOUNG, Joshua K.
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.
摘要翻译：提供组合物和方法用于植物或植物细胞基因组中靶序列的基因组修饰。方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法的细胞或生物体。一旦确定了基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

8. 发明公开

EP2807257A1 METHODS AND COMPOSITIONS FOR GENERATING COMPLEX TRAIT LOCI 审中-公开
标题翻译： VERFAHREN UND ZUSAMMENSETZUNGEN ZUR ERZEUGUNG KOMPLEXER TRAIT LOCI
公开(公告)号：EP2807257A1
公开(公告)日：2014-12-03
申请号：EP13702566.4
申请日：2013-01-24
申请人： Pioneer-Hi-Bred International, Inc. , E. I. Du Pont de Nemours and Company
发明人： FALCO, Saverio Carl , LASSNER, Michael , LI, Zhongsen , SCELONGE, Christopher J.
IPC分类号： C12N15/82 , C12N9/22
CPC分类号： A01H5/10 , A01H1/02 , C12N9/22 , C12N15/8213
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供了将多个独立的转基因基因座堆叠到植物基因组中的组合物和方法。组合物包括植物，种子或植物细胞，其包含至少一个转基因靶位点和在基因组窗口内的不同基因组位点处整合的至少一个感兴趣的基因组。可以使用植物育种技术，使得转基因靶位点和感兴趣的基因组座位可以一起繁殖。以这种方式，可以在基因组窗口内产生多个独立的转基因整合以产生复杂的性状基因座。复杂特征位点被设计成使得目的基因座和/或基因座位点彼此独立地分离，从而提供通过繁殖和遗传特异性元件改变复杂性状基因座的益处。还可以使用各种方法来修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

9. 发明公开

EP2739739A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 审中-公开
标题翻译：方法和组合物有针对性地引入到植物
公开(公告)号：EP2739739A1
公开(公告)日：2014-06-11
申请号：EP12741436.5
申请日：2012-07-18
申请人： E. I. Du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

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