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1. 发明公开

EP3628739A1 GENERATION OF SITE-SPECIFIC-INTEGRATION SITES FOR COMPLEX TRAIT LOCI IN CORN AND SOYBEAN, AND METHODS OF USE 审中-实审转让
公开(公告)号：EP3628739A1
公开(公告)日：2020-04-01
申请号：EP19206649.6
申请日：2015-08-31
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , GAO, Huirong , LIU, Zhan-Bin , MUTTI, Jasdeep , PODLICH, Dean , SCELONGE, Christopher
IPC分类号： C12N15/82 , A01H5/10
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.

4. 发明公开

EP3036332A1 PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 有权转让
标题翻译： PFLANZENGENOMMODIFIZIERUNG MIT RNS / CAS-ENDONUKLEASEFÜHRUNGSSYSTEMENUND VERFAHREN ZUR VERWENDUNG
公开(公告)号：EP3036332A1
公开(公告)日：2016-06-29
申请号：EP14761477.0
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew , FALCO, Saverio Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, L. Aleksander , SHI, Jinrui , SVITASHEV, Sergei , YOUNG, Joshua K.
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell. Breeding methods and methods for selecting plants utilizing a two component RNA polynucleotide and Cas endonuclease system are also disclosed.
摘要翻译：提供组合物和方法用于植物或植物细胞基因组中靶序列的基因组修饰。方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于细胞或生物体基因组中的核苷酸序列进行基因组修饰，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法的细胞或生物体。一旦确定了基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

5. 发明公开

EP3191595A1 GENERATION OF SITE-SPECIFIC-INTEGRATION SITES FOR COMPLEX TRAIT LOCI IN CORN AND SOYBEAN, AND METHODS OF USE 有权转让
标题翻译：用于玉米和大豆复杂性状基因位点特异性整合位点的产生和使用方法
公开(公告)号：EP3191595A1
公开(公告)日：2017-07-19
申请号：EP15763713.3
申请日：2015-08-31
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , GAO, Huirong , LIU, Zhan-Bin , MUTTI, Jasdeep , PODLICH, Dean , SCELONGE, Christopher
IPC分类号： C12N15/82 , A01H5/10
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
摘要翻译：提供组合物和方法，用于将位点特异性整合（SSI）和/或感兴趣的多核苷酸的转基因靶位点导入植物基因组的基因组窗口中的双链断裂诱导剂的至少一个双链断裂靶位点。还提供了用于在植物的基因组窗口中产生复合性状基因座的方法和组合物，其包含整合于至少双链断裂靶位点中的至少一个用于位点特异性整合的转基因靶位点。双链断裂靶位点可以是但不限于锌指内切核酸酶，工程内切核酸酶，大范围核酸酶，TALEN和/或Cas核酸内切酶的靶位点。所述植物的基因组窗口可包含至少一个感兴趣的基因组基因座，例如性状盒，转基因，突变基因，天然基因，编辑基因或位点特异性整合（SSI）靶位点。

6. 发明公开

EP2739739A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 审中-公开
标题翻译：方法和组合物有针对性地引入到植物
公开(公告)号：EP2739739A1
公开(公告)日：2014-06-11
申请号：EP12741436.5
申请日：2012-07-18
申请人： E. I. Du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.

7. 发明公开

EP3611268A1 PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 审中-公开
公开(公告)号：EP3611268A1
公开(公告)日：2020-02-19
申请号：EP19199945.7
申请日：2014-08-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
发明人： CIGAN, Andrew , FALCO, Saverio Carl , GAO, Huirong , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, L. Aleksander , SHI, Jinrui , SVITASHEV, Sergei , YOUNG, Joshua K.
IPC分类号： C12N15/82 , C12N15/113
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

8. 发明公开

EP2723874A2 METHODS AND COMPOSITIONS FOR PRODUCING MALE STERILE PLANTS 审中-公开
标题翻译： VERFAHREN UND ZUSAMMENSETZUNGEN ZUR HERSTELLUNG STERILERMÄNNLICHERPFLANZEN
公开(公告)号：EP2723874A2
公开(公告)日：2014-04-30
申请号：EP12823277.4
申请日：2012-06-19
申请人： Pioneer Hi-Bred International, Inc. , E. I. Du Pont de Nemours and Company
发明人： BIDNEY, Dennis L. , CIGAN, Mark , FALCO, Carl , GAO, Huirong , JANTZ, Derek , LASSNER, Mike , LOWE, Keith , LYZNIK, Leszek, Aleksander , SMITH, James, Jefferson
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a eel! comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand- break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译：公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。包含其中具有至少一种靶向修饰的雄性生殖力基因的核酸分子，编码作为工程改造的双链断裂诱导剂和表达盒的核酸内切酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物进一步公开。

9. 发明公开

EP4087600A1 TWO-STEP GENE SWAP 审中-公开
公开(公告)号：EP4087600A1
公开(公告)日：2022-11-16
申请号：EP21738856.0
申请日：2021-01-05
申请人： Pioneer Hi-Bred International, Inc.
发明人： GAO, Huirong , SVITASHEV, Sergei
IPC分类号： A61K38/46 , C12N9/22 , C12N15/11 , C12N15/113 , C12N15/29 , C12N15/90

10. 发明公开

EP4242330A2 GENERATING NORTHERN LEAF BLIGHT RESISTANT MAIZE 审中-公开
公开(公告)号：EP4242330A2
公开(公告)日：2023-09-13
申请号：EP23158586.0
申请日：2017-10-10
申请人： Pioneer Hi-Bred International, Inc.
发明人： GAO, Huirong , LI, Bailin , MEELEY, Robert B. , PERUGINI, Leandro Daniel , TABOR, Girma M.
IPC分类号： C12Q1/6895
摘要： Compositions and methods for obtaining plant cells with modified Ht1 nucleotide sequences, modified NLB18 sequences, or both, are provided herein. The methods involve introducing double-strand breaks into the maize genome in an endogenous Ht1 encoding sequence, an endogenous NLB18 encoding sequence, or both, to modify the genomic sequence in order to enhance northern leaf blight resistance of a plant produced from the plant cell. Further provided are methods that introduce resistance alleles of Ht1 and/or NLB18 into specific sites in the genome. Plants produced by the plant cells, and seeds produced from the plants are also included. Guide polynucleotides are also provided for the use of the CRISPR-Cas system in inducing double strand breaks.

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