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1. 发明授权

EP2925419B1 CHROMATOGRAPHIC PURIFICATION METHOD 有权
标题翻译：色谱清洁过程
公开(公告)号：EP2925419B1
公开(公告)日：2016-07-20
申请号：EP14715577.4
申请日：2014-04-03
申请人： ChromaCon AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/42 , G01N30/46 , G01N30/86
CPC分类号： B01D15/1871 , B01D15/1807 , B01D15/1864 , B01D15/3804 , B01D15/3809 , G01N30/02 , G01N30/26 , G01N30/34 , G01N30/38 , G01N30/44 , G01N30/46 , G01N30/461 , G01N30/462 , G01N30/463 , G01N30/468
摘要： A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns.

2. 发明授权

EP2772289B1 Chromatographic process for enrichment and isolation 有权
标题翻译：对于富集和分离色谱法
公开(公告)号：EP2772289B1
公开(公告)日：2015-05-06
申请号：EP13157388.3
申请日：2013-03-01
申请人： ChromaCon AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/46
CPC分类号： C07K1/165 , B01D15/1864 , B01D15/40 , C11B3/008 , G01N30/461 , G01N30/466 , G01N30/468

3. 发明公开

EP2686082A1 METHOD FOR IDENTIFICATION AND PURIFICATION OF MULTI-SPECIFIC POLYPEPTIDES 审中-公开
标题翻译：方法鉴别和清洗多特异性多肽的
公开(公告)号：EP2686082A1
公开(公告)日：2014-01-22
申请号：EP12710474.3
申请日：2012-03-15
申请人： ChromaCon AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael
IPC分类号： B01D15/18 , G01N30/46
CPC分类号： C12N15/1082 , B01D15/166 , B01D15/1807 , B01D15/1864 , C07K1/18 , C07K1/22 , C07K1/36 , C07K2317/31 , G01N30/468
摘要： The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterised in that for the separation of the three components and in particular for the isolation of the multi-specific fraction AB multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per millilitre stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method.

4. 发明公开

EP2925419A1 CHROMATOGRAPHIC PURIFICATION METHOD 有权
标题翻译：色谱清洁过程
公开(公告)号：EP2925419A1
公开(公告)日：2015-10-07
申请号：EP14715577.4
申请日：2014-04-03
申请人： ChromaCon AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/42 , G01N30/46 , G01N30/86
CPC分类号： B01D15/1871 , B01D15/1807 , B01D15/1864 , B01D15/3804 , B01D15/3809 , G01N30/02 , G01N30/26 , G01N30/34 , G01N30/38 , G01N30/44 , G01N30/46 , G01N30/461 , G01N30/462 , G01N30/463 , G01N30/468
摘要： A chromatographic purification method for the isolation of a desired product fraction from a mixture using 2 chromatographic columns, comprises, within one cycle to be carried out at least once, the following steps: a first batch step, wherein said columns are disconnected and a first column is loaded with feed and its outlet is directed to waste, and from a second column desired product is recovered and subsequently the second column is regenerated; a first interconnected step, wherein the outlet of the first column is connected to the inlet of the second column, the first column is loaded beyond its dynamic breakthrough capacity with feed, and the outlet of the second column is directed to waste, a second batch step analogous to the first batch step but with exchanged columns; and a second interconnected step, analogous to the first interconnected step but with exchanged columns.

5. 发明公开

EP2772289A1 Chromatographic process for enrichment and isolation 有权
标题翻译： Chromatographieverfahren zur Anreicherung und Isolierung
公开(公告)号：EP2772289A1
公开(公告)日：2014-09-03
申请号：EP13157388.3
申请日：2013-03-01
申请人： ChromaCon AG
发明人： MÜLLER-SPÄTH, Thomas , AUMANN, Lars , STRÖHLEIN, Guido , BAVAND, Michael , ULMER, Nicole
IPC分类号： B01D15/18 , G01N30/46
CPC分类号： C07K1/165 , B01D15/1864 , B01D15/40 , C11B3/008 , G01N30/461 , G01N30/466 , G01N30/468
摘要： A chromatographic process for the enrichment of at least one compound of interest (X) from a mixture (F) comprising said at least one compound of interest (X) as well as at least one further compound (W, C, S), which is not of interest, is proposed, using at least two chromatographic columns, wherein said process at least involves a sequence of the following steps:
(i) a cyclic accumulation phase, in which the at least two chromatographic columns are alternatingly operated in an interconnected phase (IC), followed by a disconnected phase (B), wherein after these two phases the first and second column or group of columns exchange places; wherein said two phases are carried out sequentially M times with M > 1;
(ii) a cyclic separation phase, in which the at least two chromatographic columns are alternatingly operated in an interconnected phase (IC), followed by a disconnected phase (B), wherein after these two phases the first and second column or group of columns exchange places to undergo the next interconnected (IC) and disconnected phases (B); wherein said two phases are carried out sequentially N times with N ≥ 0;
(iii) an elution phase, in which from the column or group of columns, which at the end of phase (i) or, in case of N>0 at the end of phase (ii) contains the at least one compound of interest (X), said at least one compound of interest (X) is extracted via the outlet.
摘要翻译：用于从包含所述至少一种目的化合物（X）的混合物（F）以及至少一种其它化合物（W，C，S）富集至少一种目的化合物（X）的色谱方法，其中不建议使用至少两个色谱柱，其中所述方法至少涉及以下步骤的顺序：（i）循环累积阶段，其中所述至少两个色谱柱交替地在相互连接的操作中相位（IC），之后是断开相位（B），其中在这两个阶段之后，第一和第二列或一组列交换位置; 其中所述两相按M> 1顺序执行M次; （ii）循环分离阶段，其中至少两个色谱柱在互连相（IC）中交替操作，随后是断开相（B），其中在这两相之后，第一和第二列或一组柱交换场所进行下一个互连（IC）和断开阶段（B）; 其中所述两相按N‰¥0顺序进行N次; （iii）洗脱阶段，其中在阶段（i）结束时或在阶段（ii）结束时的N> 0的情况下从列或组列中含有至少一种目标化合物（X），所述至少一种感兴趣的化合物（X）经由所述出口提取。

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