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1. 发明公开

EP2961763A1 CHROMATOGRAPHIC PURIFICATION OF ANTIBODIES FROM CHROMATIN-DEFICIENT CELL CULTURE HARVESTS 审中-公开
标题翻译：抗体从收入CHROMATINMANGEL细胞培养色谱清洗
公开(公告)号：EP2961763A1
公开(公告)日：2016-01-06
申请号：EP14756552.7
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley , LEE, Tze Yang
IPC分类号： C07K1/30 , C07K1/36
CPC分类号： C07K1/36 , C07K1/30 , C07K1/32 , C07K16/32
摘要： Methods for the improved purification of antibodies and other proteins from protein preparations including the steps of conditioning the protein preparation by contacting it with multivalent organic ions, then applying the conditioned preparation to an adsorptive chromatography medium.

2. 发明公开

EP2961759A1 PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES 审中-公开
标题翻译：蛋白质清洁在非离子有机聚合物和电正完成PRESENCE
公开(公告)号：EP2961759A1
公开(公告)日：2016-01-06
申请号：EP14756237.5
申请日：2014-02-26
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14 , B01D15/36 , C07K1/30
CPC分类号： C07K1/165 , B01D15/305 , B01D15/363 , B01D15/3847 , C07K1/30 , C07K1/303 , B01D15/327 , B01D15/34
摘要： A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

3. 发明公开

EP2953964A1 METHODS FOR REDUCING AGGREGATE LEVELS IN PROTEIN PREPARATIONS BY TREATMENT WITH THIO-HETEROCYCLIC CATIONS 审中-公开
标题翻译：程序中的蛋白质制品总量水平对通过用硫杂环阳离子还原
公开(公告)号：EP2953964A1
公开(公告)日：2015-12-16
申请号：EP14749160.9
申请日：2014-02-05
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/22 , B01D15/16 , C07K16/00 , C07D279/18
CPC分类号： C07K1/14 , B01D15/327 , B01D15/34 , B01D15/363 , B01D15/3828 , C07K1/165 , C07K1/20 , C07K16/00 , C07K16/3015
摘要： A method of reducing the aggregate content in a protein preparation having a target protein includes contacting the protein preparation with a thio-heterocyclic cation to form a mixture, contacting the mixture with at least one functionalized solid to remove excess thio-heterocyclic cations; and optionally contacting the mixture, simultaneously or sequentially, with at least one further functionalized solid to further reduce aggregate content of the protein preparation.

4. 发明授权

EP2961761B1 PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY 有权
公开(公告)号：EP2961761B1
公开(公告)日：2018-09-12
申请号：EP14757178.0
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14 , C07K16/06 , C07K1/16 , C07K1/30 , C07K16/36
CPC分类号： C07K1/16 , C07K1/30 , C07K16/065 , C07K16/36
摘要： A method of purifying a desired protein from a preparation includes providing the preparation in a form having less than about 5% of the chromatin residing in the original production medium, contacting the preparation with a nonionic organic polymer in an amount sufficient to cause the desired protein to precipitate or adsorb on a nonionic hydrophilic surface, and adjusting a salt concentration before or during the contacting step, the adjusting step providing a sufficient salt concentration to produce a conductivity greater than physiological conductivity.

5. 发明公开

EP3149023A1 Protein Extraction Methods 审中-公开
标题翻译： PROTEINEXTRAKTIONSVERFAHREN
公开(公告)号：EP3149023A1
公开(公告)日：2017-04-05
申请号：EP14893573.7
申请日：2014-05-29
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley , GAN, Hui Theng
IPC分类号： C07K1/18 , C07K1/36
CPC分类号： C07K14/4702 , B01D15/12 , B01D15/34 , B01D15/363 , C07K1/36 , C07K14/4703
摘要： Methods are provided for extracting DNA-compaction proteins from biological samples.
摘要翻译：提供了从生物样品中提取DNA-压实蛋白的方法。

6. 发明公开

EP3149022A1 VIRUS REDUCTION METHOD 审中-公开
标题翻译： VIRUSREDUKTIONSVERFAHREN
公开(公告)号：EP3149022A1
公开(公告)日：2017-04-05
申请号：EP14893096.9
申请日：2014-05-28
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14
CPC分类号： C07K1/18 , B01J41/20
摘要： Methods are provided for enhancing reduction of virus and viral DNA levels in protein preparations.
摘要翻译：提供了用于增强蛋白质制剂中病毒和病毒DNA水平的降低的方法。

7. 发明公开

EP3110830A1 METHODS FOR REDUCING CHROMATIN CONTENT IN PROTEIN PREPARATIONS BY TREATMENT WITH ALKYL CATIONS 审中-公开
标题翻译：维他命ZUR REDUZIERUNG DES蛋白质在蛋白质去甲肾上腺素麻醉药物中的应用
公开(公告)号：EP3110830A1
公开(公告)日：2017-01-04
申请号：EP14883801.4
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/16 , B01D15/26
CPC分类号： C07K1/32 , B01D15/265 , B01D15/327 , B01D15/361 , B01D15/3828 , C07K1/145 , C07K1/36 , C07K16/00
摘要： A method of reducing aggregate content in a preparation having a target protein includes contacting the preparation with an alkyl cation to form a mixture, and contacting the mixture with at least one functionalized solid to remove excess alkyl cation.
摘要翻译：减少具有靶蛋白的制剂中骨料含量的方法包括使制剂与烷基阳离子接触以形成混合物，并使该混合物与至少一种官能化固体接触以除去过量的烷基阳离子。

8. 发明公开

EP3110829A1 ANTIBODY PURIFICATION PROCESS 审中-公开
标题翻译： ANTIKÖRPERREINIGUNGSVERFAHREN
公开(公告)号：EP3110829A1
公开(公告)日：2017-01-04
申请号：EP14883697.6
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/14
CPC分类号： C07K1/30 , C07K1/32 , C07K16/00
摘要： A method of purifying a target antibody includes contacting a cell culture harvest or a protein preparation including at least one target antibody with at least one fatty acid having 7 to 10 carbon atoms to form a mixture, contacting this mixture with allantoin, and then separating solid materials to provide a solution comprising the target antibody. Solid materials can be removed by filtration, sedimentation or centrifugation, and the fatty acids can be enanthic, caprylic, pelargonic, nonenoic or capric acid. The invention is also directed to kits used to facilitate this method of antibody purification.
摘要翻译：纯化靶抗体的方法包括将细胞培养物收获物或包含至少一种靶抗体的蛋白质制剂与至少一种具有7至10个碳原子的脂肪酸接触以形成混合物，使该混合物与尿囊素接触，然后分离固体提供包含靶抗体的溶液的材料。固体材料可以通过过滤，沉淀或离心去除，脂肪酸可以是甘油，辛酸，壬酸，壬烯或癸酸。本发明还涉及用于促进抗体纯化方法的试剂盒。

9. 发明公开

EP3348565A1 FRACTIONATION METHOD 审中-公开
公开(公告)号：EP3348565A1
公开(公告)日：2018-07-18
申请号：EP18158709.8
申请日：2015-02-17
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/34 , C07K16/32 , C07K1/30 , C07K1/18 , C07K1/22 , C07K1/36 , C07K16/06 , C07K16/00
CPC分类号： C07K1/36 , C07K1/18 , C07K1/22 , C07K1/303 , C07K1/34 , C07K16/00 , C07K16/065 , C07K16/32
摘要： A method of purifying an IgG antibody from a preparation which includes providing the preparation in a form having substantially reduced chromatin levels from that of the sample from which the preparation was obtained and contacting the preparation with an electropositive membrane having a porosity that retains at least 50% of non-adsorbed solutes with a hydrodynamic diameter greater than a selected size but permits passage of non-adsorbed solutes with a hydrodynamic diameter less than the selected size, wherein the selected size is between about 10 nm and about 15 nm, where during at least a portion of the contacting step the preparation includes a salt such that (1) when present at a concentration less than 50 mM, a pH value is in a range from about 3 to within 0.5 pH units of the isoelectric point of the most alkaline glycoform of the IgG antibody in the preparation; or (2) when present at concentration greater than 50 mM, a pH value of the preparation is in a range from 3 to 9,, and adjusting operating conditions to the highest pH and lowest salt concentration that does not result in more than 5% of the antibody being bound to the membrane.

10. 发明公开

EP3004135A1 PROTEIN PURIFICATION PROCESS 审中-公开
标题翻译：蛋白质纯化过程
公开(公告)号：EP3004135A1
公开(公告)日：2016-04-13
申请号：EP14807348.9
申请日：2014-02-27
申请人： Agency for Science, Technology and Research
发明人： GAGNON, Peter Stanley
IPC分类号： C07K1/30 , C07K1/36
CPC分类号： C07K1/36 , C07K1/30 , C07K1/34 , C07K16/00 , C07K16/32
摘要： A method of purifying a target protein includes contacting a cell culture harvest or a protein preparation including at least one target protein with at least one fatty acid having 8 to 10 carbon atoms to form a mixture, contacting the mixture with one or more solids to form a mixture, the one or more solids comprise a cationic functional group, a metal binding functional group, or both, the metal binding functional group including a nitrogen-containing moiety selected from (1) a polyamine, (2) an imine, (3) an
N -heterocycle, (4) an amino acid, (5) an
N -hydroxyamide, (6), an arylamine, and combinations thereof, and separating solid materials after contacting the mixture with the one or more solids to provide a solution comprising the target protein.
摘要翻译：纯化靶蛋白的方法包括使细胞培养收获物或包含至少一种靶蛋白的蛋白制剂与至少一种具有8至10个碳原子的脂肪酸接触以形成混合物，使该混合物与一种或多种固体接触以形成所述一种或多种固体包含阳离子官能团，金属结合官能团或两者，所述金属结合官能团包括选自以下的含氮部分：（1）多胺，（2）亚胺，（3））N-杂环，（4）氨基酸，（5）N-羟基酰胺，（6），芳基胺及其组合，并且在将混合物与一种或多种固体接触后分离固体材料以提供溶液包含靶蛋白。

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